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Copper in PDB 9fuk: Crystal Structure of Nitric Oxide-Treated Q262N Mutant of Three-Domain Heme-Cu Nitrite Reductase From Ralstonia Pickettii

Enzymatic activity of Crystal Structure of Nitric Oxide-Treated Q262N Mutant of Three-Domain Heme-Cu Nitrite Reductase From Ralstonia Pickettii

All present enzymatic activity of Crystal Structure of Nitric Oxide-Treated Q262N Mutant of Three-Domain Heme-Cu Nitrite Reductase From Ralstonia Pickettii:
1.7.2.1;

Protein crystallography data

The structure of Crystal Structure of Nitric Oxide-Treated Q262N Mutant of Three-Domain Heme-Cu Nitrite Reductase From Ralstonia Pickettii, PDB code: 9fuk was solved by N.Petchyam, S.Antonyuk, S.S.Hasnain, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 37.74 / 1.09
Space group H 3
Cell size a, b, c (Å), α, β, γ (°) 128.069, 128.069, 86.352, 90, 90, 120
R / Rfree (%) 10.7 / 12.2

Other elements in 9fuk:

The structure of Crystal Structure of Nitric Oxide-Treated Q262N Mutant of Three-Domain Heme-Cu Nitrite Reductase From Ralstonia Pickettii also contains other interesting chemical elements:

Iron (Fe) 1 atom

Copper Binding Sites:

The binding sites of Copper atom in the Crystal Structure of Nitric Oxide-Treated Q262N Mutant of Three-Domain Heme-Cu Nitrite Reductase From Ralstonia Pickettii (pdb code 9fuk). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 3 binding sites of Copper where determined in the Crystal Structure of Nitric Oxide-Treated Q262N Mutant of Three-Domain Heme-Cu Nitrite Reductase From Ralstonia Pickettii, PDB code: 9fuk:
Jump to Copper binding site number: 1; 2; 3;

Copper binding site 1 out of 3 in 9fuk

Go back to Copper Binding Sites List in 9fuk
Copper binding site 1 out of 3 in the Crystal Structure of Nitric Oxide-Treated Q262N Mutant of Three-Domain Heme-Cu Nitrite Reductase From Ralstonia Pickettii


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of Crystal Structure of Nitric Oxide-Treated Q262N Mutant of Three-Domain Heme-Cu Nitrite Reductase From Ralstonia Pickettii within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cu501

b:9.8
occ:1.00
ND1 A:HIS94 2.0 9.9 1.0
ND1 A:HIS143 2.1 9.6 1.0
SG A:CYS135 2.2 10.0 1.0
SD A:MET148 2.6 9.7 1.0
HB3 A:HIS143 2.9 8.7 1.0
CE1 A:HIS143 2.9 9.2 1.0
CE1 A:HIS94 3.0 9.6 1.0
HE1 A:HIS143 3.1 9.2 1.0
CG A:HIS143 3.1 9.0 1.0
HE1 A:HIS94 3.1 9.7 1.0
CG A:HIS94 3.1 9.6 1.0
HA A:HIS94 3.1 9.6 1.0
HB3 A:HIS94 3.1 9.7 1.0
HB2 A:CYS135 3.2 9.0 1.0
CB A:CYS135 3.2 9.0 1.0
HE3 A:MET148 3.2 10.8 1.0
HB3 A:CYS135 3.4 9.0 1.0
CE A:MET148 3.4 11.1 1.0
CB A:HIS94 3.5 9.7 1.0
CB A:HIS143 3.5 8.7 1.0
HB A:THR137 3.5 9.8 1.0
HE1 A:MET148 3.6 10.8 1.0
CA A:HIS94 3.8 9.5 1.0
H A:ASN95 3.9 9.5 1.0
HE3 A:TRP61 3.9 9.7 1.0
O A:PRO93 4.0 10.3 1.0
NE2 A:HIS143 4.1 9.2 1.0
CG A:MET148 4.1 9.9 1.0
HB2 A:HIS143 4.1 8.7 1.0
NE2 A:HIS94 4.1 10.1 1.0
CD2 A:HIS143 4.2 9.2 1.0
CD2 A:HIS94 4.2 9.6 1.0
HE2 A:MET148 4.3 10.8 1.0
OG1 A:THR137 4.3 10.7 1.0
CB A:THR137 4.3 9.7 1.0
HG3 A:MET148 4.4 9.7 1.0
HB2 A:HIS94 4.4 9.6 1.0
HB3 A:MET148 4.4 9.1 1.0
HA A:HIS143 4.5 8.6 1.0
HB2 A:MET148 4.5 9.1 1.0
CE3 A:TRP61 4.6 9.8 1.0
H A:THR137 4.6 9.5 1.0
CA A:HIS143 4.6 8.5 1.0
CB A:MET148 4.6 9.2 1.0
CA A:CYS135 4.6 8.5 1.0
N A:ASN95 4.6 9.5 1.0
HZ3 A:TRP61 4.8 10.0 1.0
N A:HIS94 4.8 9.8 1.0
HB3 A:TRP61 4.8 9.5 1.0
HG2 A:MET148 4.8 9.7 1.0
C A:HIS94 4.8 9.8 1.0
HG21 A:THR137 4.8 10.0 1.0
HG13 A:VAL45 4.8 11.1 1.0
HA A:CYS135 4.8 8.7 1.0
C A:PRO93 4.8 10.1 1.0
H A:ALA136 5.0 8.9 1.0

Copper binding site 2 out of 3 in 9fuk

Go back to Copper Binding Sites List in 9fuk
Copper binding site 2 out of 3 in the Crystal Structure of Nitric Oxide-Treated Q262N Mutant of Three-Domain Heme-Cu Nitrite Reductase From Ralstonia Pickettii


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of Crystal Structure of Nitric Oxide-Treated Q262N Mutant of Three-Domain Heme-Cu Nitrite Reductase From Ralstonia Pickettii within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cu502

b:9.9
occ:0.70
CU A:CU502 0.0 9.9 0.7
CU A:CU502 0.5 10.1 0.3
NE2 A:HIS99 1.9 11.5 1.0
NE2 A:HIS134 2.1 10.1 1.0
O A:HOH652 2.2 13.4 1.0
CE1 A:HIS99 2.8 11.7 1.0
CD2 A:HIS134 2.9 9.1 1.0
CD2 A:HIS99 3.0 10.5 1.0
HE1 A:HIS99 3.0 11.5 1.0
HD2 A:HIS134 3.0 9.0 1.0
CE1 A:HIS134 3.2 9.7 1.0
O A:HOH1153 3.2 25.7 0.5
HD2 A:HIS99 3.2 10.7 1.0
HE1 A:HIS134 3.5 9.7 1.0
ND1 A:HIS99 4.0 11.7 1.0
OD1 A:ASP97 4.0 10.2 1.0
CG A:HIS99 4.1 10.7 1.0
CG A:HIS134 4.1 8.3 1.0
ND1 A:HIS134 4.2 9.6 1.0
CG A:ASP97 4.6 9.7 1.0
HG12 A:VAL132 4.7 9.4 1.0
HA3 A:GLY269 4.7 8.9 1.0
OD2 A:ASP97 4.9 10.5 1.0
HB3 A:ASP97 4.9 9.0 1.0

Copper binding site 3 out of 3 in 9fuk

Go back to Copper Binding Sites List in 9fuk
Copper binding site 3 out of 3 in the Crystal Structure of Nitric Oxide-Treated Q262N Mutant of Three-Domain Heme-Cu Nitrite Reductase From Ralstonia Pickettii


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 3 of Crystal Structure of Nitric Oxide-Treated Q262N Mutant of Three-Domain Heme-Cu Nitrite Reductase From Ralstonia Pickettii within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cu502

b:10.1
occ:0.30
CU A:CU502 0.0 10.1 0.3
CU A:CU502 0.5 9.9 0.7
O A:HOH652 1.8 13.4 1.0
NE2 A:HIS134 1.9 10.1 1.0
NE2 A:HIS99 2.3 11.5 1.0
O A:HOH1153 2.8 25.7 0.5
CE1 A:HIS134 2.9 9.7 1.0
CD2 A:HIS134 3.0 9.1 1.0
HE1 A:HIS134 3.0 9.7 1.0
HD2 A:HIS134 3.2 9.0 1.0
CD2 A:HIS99 3.2 10.5 1.0
CE1 A:HIS99 3.3 11.7 1.0
HD2 A:HIS99 3.3 10.7 1.0
HE1 A:HIS99 3.5 11.5 1.0
OD1 A:ASP97 3.6 10.2 1.0
ND1 A:HIS134 4.0 9.6 1.0
CG A:HIS134 4.1 8.3 1.0
CG A:ASP97 4.3 9.7 1.0
CG A:HIS99 4.4 10.7 1.0
ND1 A:HIS99 4.4 11.7 1.0
HB3 A:ASP97 4.6 9.0 1.0
OD2 A:ASP97 4.7 10.5 1.0

Reference:

N.Petchyam, S.Antonyuk, S.S.Hasnain. Structural Studies of Haem Three-Domain Copper Nitrite Reductase Mutants From Ralstonia Pickettii To Be Published.
Page generated: Mon Jul 14 09:55:57 2025

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