Copper in PDB 7qxk: As Isolated Msox Movie Series Dataset 1 (0.4 Mgy) of the Copper Nitrite Reductase From Bradyrhizobium Sp. Ors 375 (Two-Domain)

All present enzymatic activity of As Isolated Msox Movie Series Dataset 1 (0.4 Mgy) of the Copper Nitrite Reductase From Bradyrhizobium Sp. Ors 375 (Two-Domain):
1.7.2.1;

The structure of As Isolated Msox Movie Series Dataset 1 (0.4 Mgy) of the Copper Nitrite Reductase From Bradyrhizobium Sp. Ors 375 (Two-Domain), PDB code: 7qxk was solved by S.L.Rose, S.Baba, H.Okumura, S.V.Antonyuk, D.Sasaki, T.Tosha, T.Kumasaka, R.R.Eady, M.Yamamoto, S.S.Hasnain, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	47.99 / 1.35
Space group	P 21 3
Cell size a, b, c (Å), α, β, γ (°)	107.215, 107.215, 107.215, 90, 90, 90
R / Rfree (%)	12.4 / 14.5

The binding sites of Copper atom in the As Isolated Msox Movie Series Dataset 1 (0.4 Mgy) of the Copper Nitrite Reductase From Bradyrhizobium Sp. Ors 375 (Two-Domain) (pdb code 7qxk). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 2 binding sites of Copper where determined in the As Isolated Msox Movie Series Dataset 1 (0.4 Mgy) of the Copper Nitrite Reductase From Bradyrhizobium Sp. Ors 375 (Two-Domain), PDB code: 7qxk:
Jump to Copper binding site number: 1; 2;

Copper binding site 1 out of 2 in the As Isolated Msox Movie Series Dataset 1 (0.4 Mgy) of the Copper Nitrite Reductase From Bradyrhizobium Sp. Ors 375 (Two-Domain)


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of As Isolated Msox Movie Series Dataset 1 (0.4 Mgy) of the Copper Nitrite Reductase From Bradyrhizobium Sp. Ors 375 (Two-Domain) within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu406 b:12.2 occ:1.00 ND1 A:HIS140 2.0 12.2 1.0 ND1 A:HIS89 2.0 9.7 1.0 SG A:CYS130 2.2 11.8 1.0 SD A:MET145 2.5 12.4 1.0 CE1 A:HIS89 2.9 10.9 1.0 CE1 A:HIS140 2.9 11.1 1.0 CG A:HIS140 3.1 12.1 1.0 CG A:HIS89 3.1 11.1 1.0 CB A:CYS130 3.2 10.8 1.0 CE A:MET145 3.3 12.8 1.0 CB A:HIS140 3.4 10.5 1.0 CB A:HIS89 3.5 11.4 1.0 CA A:HIS89 3.8 11.4 1.0 CG A:MET145 4.0 11.3 1.0 O A:PRO88 4.1 13.2 1.0 NE2 A:HIS140 4.1 12.8 1.0 CG A:PRO132 4.1 12.2 0.7 NE2 A:HIS89 4.1 12.0 1.0 CD2 A:HIS140 4.2 13.8 1.0 CD2 A:HIS89 4.2 11.4 1.0 CG A:PRO132 4.4 11.2 0.3 CB A:MET145 4.5 11.4 1.0 CD A:PRO132 4.6 11.5 0.7 SD A:MET56 4.6 13.1 0.5 CA A:CYS130 4.6 10.7 1.0 N A:ASN90 4.6 12.1 1.0 CA A:HIS140 4.7 10.5 1.0 CD A:PRO132 4.8 11.2 0.3 C A:HIS89 4.8 12.1 1.0 N A:HIS89 4.8 11.8 1.0 C A:PRO88 4.9 12.0 1.0

Copper binding site 2 out of 2 in the As Isolated Msox Movie Series Dataset 1 (0.4 Mgy) of the Copper Nitrite Reductase From Bradyrhizobium Sp. Ors 375 (Two-Domain)


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of As Isolated Msox Movie Series Dataset 1 (0.4 Mgy) of the Copper Nitrite Reductase From Bradyrhizobium Sp. Ors 375 (Two-Domain) within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu407 b:11.7 occ:0.90 O A:HOH501 1.9 26.6 1.0 NE2 A:HIS94 2.0 10.3 1.0 NE2 A:HIS129 2.1 11.4 1.0 CE1 A:HIS94 3.0 10.2 1.0 OD2 A:ASP92 3.0 19.2 0.3 CD2 A:HIS129 3.1 10.9 1.0 CD2 A:HIS94 3.1 9.3 1.0 CE1 A:HIS129 3.1 11.0 1.0 OD2 A:ASP92 3.8 16.3 0.7 O A:HOH578 4.0 13.5 0.3 O A:HOH578 4.1 20.6 0.7 ND1 A:HIS94 4.1 9.4 1.0 CG A:ASP92 4.2 16.8 0.3 CG A:HIS94 4.2 9.2 1.0 CG A:HIS129 4.2 10.5 1.0 ND1 A:HIS129 4.3 10.7 1.0 CG A:ASP92 4.4 14.9 0.7 OD1 A:ASP92 4.5 14.9 0.7 OD1 A:ASP92 4.8 17.7 0.3 O A:HOH776 4.9 16.0 1.0

S.L.Rose, S.Baba, H.Okumura, S.V.Antonyuk, D.Sasaki, T.M.Hedison, M.Shanmugam, D.J.Heyes, N.S.Scrutton, T.Kumasaka, T.Tosha, R.R.Eady, M.Yamamoto, S.S.Hasnain. Single Crystal Spectroscopy and Multiple Structures From One Crystal (Msox) Define Catalysis in Copper Nitrite Reductases. Proc.Natl.Acad.Sci.Usa V. 119 64119 2022.
ISSN: ESSN 1091-6490
PubMed: 35862453
DOI: 10.1073/PNAS.2205664119