Copper in PDB 6juc: Aspergillus Oryzae Pro-Tyrosinase Oxygen-Bound C92A/H103F Mutant

All present enzymatic activity of Aspergillus Oryzae Pro-Tyrosinase Oxygen-Bound C92A/H103F Mutant:
1.14.18.1;

The structure of Aspergillus Oryzae Pro-Tyrosinase Oxygen-Bound C92A/H103F Mutant, PDB code: 6juc was solved by N.Fujieda, K.Umakoshi, Y.Nishikawa, G.Kurisu, S.Itoh, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.00 / 1.44
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	54.118, 118.027, 79.071, 90.00, 91.21, 90.00
R / Rfree (%)	17 / 22.5

The binding sites of Copper atom in the Aspergillus Oryzae Pro-Tyrosinase Oxygen-Bound C92A/H103F Mutant (pdb code 6juc). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 4 binding sites of Copper where determined in the Aspergillus Oryzae Pro-Tyrosinase Oxygen-Bound C92A/H103F Mutant, PDB code: 6juc:
Jump to Copper binding site number: 1; 2; 3; 4;

Copper binding site 1 out of 4 in the Aspergillus Oryzae Pro-Tyrosinase Oxygen-Bound C92A/H103F Mutant


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of Aspergillus Oryzae Pro-Tyrosinase Oxygen-Bound C92A/H103F Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu701 b:18.8 occ:0.51 O A:HOH1034 1.5 27.6 1.0 NE2 A:HIS94 2.0 20.7 1.0 NE2 A:HIS67 2.0 15.9 1.0 CE1 A:HIS67 2.9 15.3 1.0 CD2 A:HIS94 3.0 20.2 1.0 CE1 A:HIS94 3.0 20.2 1.0 CD2 A:HIS67 3.1 14.5 1.0 CZ A:PHE103 3.3 13.1 1.0 CE2 A:PHE513 3.6 17.0 1.0 CU A:CU702 3.8 18.7 0.8 ND1 A:HIS67 4.0 15.6 1.0 CE1 A:PHE103 4.1 14.9 1.0 ND1 A:HIS94 4.1 19.4 1.0 CE2 A:PHE103 4.1 12.4 1.0 CG A:HIS94 4.1 18.0 1.0 CG A:HIS67 4.2 13.7 1.0 CZ A:PHE513 4.2 16.1 1.0 CE2 A:PHE368 4.5 14.4 1.0 NE2 A:HIS328 4.5 15.4 1.0 NE2 A:HIS372 4.6 14.5 1.0 CD2 A:PHE513 4.6 16.9 1.0 CB A:ALA92 4.6 17.5 1.0 CZ A:PHE368 4.7 14.2 1.0 CE1 A:HIS328 4.7 15.1 1.0 NE2 A:HIS332 4.8 14.9 1.0 CG1 A:VAL359 4.9 23.2 1.0

Copper binding site 2 out of 4 in the Aspergillus Oryzae Pro-Tyrosinase Oxygen-Bound C92A/H103F Mutant


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of Aspergillus Oryzae Pro-Tyrosinase Oxygen-Bound C92A/H103F Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu702 b:18.7 occ:0.83 NE2 A:HIS332 2.0 14.9 1.0 NE2 A:HIS372 2.0 14.5 1.0 NE2 A:HIS328 2.2 15.4 1.0 O A:HOH1034 2.3 27.6 1.0 CE1 A:HIS372 2.9 14.4 1.0 CD2 A:HIS372 2.9 13.5 1.0 CE1 A:HIS332 3.0 14.8 1.0 CD2 A:HIS328 3.0 16.1 1.0 CD2 A:HIS332 3.0 13.8 1.0 CE1 A:HIS328 3.2 15.1 1.0 CE2 A:PHE368 3.7 14.4 1.0 CU A:CU701 3.8 18.8 0.5 ND1 A:HIS372 4.0 13.5 1.0 CG A:HIS372 4.1 13.6 1.0 ND1 A:HIS332 4.1 12.3 1.0 CG A:HIS332 4.1 13.7 1.0 CG A:HIS328 4.2 14.6 1.0 CE2 A:PHE513 4.2 17.0 1.0 ND1 A:HIS328 4.2 15.1 1.0 CD2 A:PHE368 4.3 12.8 1.0 CD2 A:HIS371 4.3 13.1 1.0 CZ A:PHE368 4.4 14.2 1.0 CZ A:PHE513 4.5 16.1 1.0 NE2 A:HIS371 4.7 11.9 1.0 CE1 A:PHE103 4.7 14.9 1.0 CZ A:PHE103 4.7 13.1 1.0 NE2 A:HIS67 4.9 15.9 1.0

Copper binding site 3 out of 4 in the Aspergillus Oryzae Pro-Tyrosinase Oxygen-Bound C92A/H103F Mutant


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 3 of Aspergillus Oryzae Pro-Tyrosinase Oxygen-Bound C92A/H103F Mutant within 5.0Å range: probe atom residue distance (Å) B Occ B:Cu701 b:16.1 occ:0.66 O1 B:PER703 1.6 20.5 0.8 O2 B:PER703 1.9 17.8 0.8 NE2 B:HIS94 1.9 18.0 1.0 NE2 B:HIS67 2.0 13.9 1.0 CE1 B:HIS67 2.8 14.9 1.0 CE1 B:HIS94 2.9 18.0 1.0 CD2 B:HIS94 3.0 18.6 1.0 CD2 B:HIS67 3.2 14.1 1.0 CZ B:PHE103 3.2 15.4 1.0 CU B:CU702 3.5 19.1 0.9 CE2 B:PHE513 3.6 15.8 1.0 CE2 B:PHE103 4.0 15.1 1.0 CE1 B:PHE103 4.0 15.2 1.0 ND1 B:HIS94 4.0 18.1 1.0 ND1 B:HIS67 4.0 14.4 1.0 CG B:HIS94 4.1 19.0 1.0 CG B:HIS67 4.2 13.8 1.0 CZ B:PHE513 4.2 15.3 1.0 CB B:ALA92 4.5 17.6 1.0 CE2 B:PHE368 4.5 15.1 1.0 NE2 B:HIS328 4.6 15.1 1.0 NE2 B:HIS372 4.6 15.5 1.0 CD2 B:PHE513 4.7 15.4 1.0 CE1 B:HIS328 4.7 16.5 1.0 CZ B:PHE368 4.7 17.4 1.0 NE2 B:HIS332 4.8 15.2 1.0

Copper binding site 4 out of 4 in the Aspergillus Oryzae Pro-Tyrosinase Oxygen-Bound C92A/H103F Mutant


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 4 of Aspergillus Oryzae Pro-Tyrosinase Oxygen-Bound C92A/H103F Mutant within 5.0Å range: probe atom residue distance (Å) B Occ B:Cu702 b:19.1 occ:0.87 O2 B:PER703 1.8 17.8 0.8 NE2 B:HIS328 2.0 15.1 1.0 NE2 B:HIS332 2.1 15.2 1.0 NE2 B:HIS372 2.1 15.5 1.0 O1 B:PER703 2.3 20.5 0.8 CE1 B:HIS328 2.9 16.5 1.0 CD2 B:HIS328 2.9 14.7 1.0 CE1 B:HIS372 3.0 14.1 1.0 CD2 B:HIS332 3.0 13.4 1.0 CE1 B:HIS332 3.0 14.4 1.0 CD2 B:HIS372 3.2 17.2 1.0 CU B:CU701 3.5 16.1 0.7 CE2 B:PHE368 4.0 15.1 1.0 CE2 B:PHE513 4.0 15.8 1.0 ND1 B:HIS328 4.0 15.8 1.0 CG B:HIS328 4.1 15.7 1.0 ND1 B:HIS372 4.1 14.2 1.0 ND1 B:HIS332 4.1 13.6 1.0 CG B:HIS332 4.2 11.6 1.0 CG B:HIS372 4.2 14.0 1.0 CZ B:PHE103 4.4 15.4 1.0 CZ B:PHE513 4.4 15.3 1.0 CZ B:PHE368 4.5 17.4 1.0 CE1 B:PHE103 4.5 15.2 1.0 CD2 B:PHE368 4.6 12.8 1.0 CD2 B:HIS371 4.6 14.4 1.0 NE2 B:HIS67 4.8 13.9 1.0 CE1 B:PHE99 4.8 13.3 1.0 CD2 B:PHE513 4.9 15.4 1.0 NE2 B:HIS371 4.9 13.5 1.0 CE2 B:PHE103 5.0 15.1 1.0

N.Fujieda, K.Umakoshi, Y.Ochi, Y.Nishikawa, S.Yanagisawa, M.Kubo, G.Kurisu, S.Itoh. Copper-Oxygen Dynamics in Tyrosinase Mechanism. Angew.Chem.Int.Ed.Engl. 2020.
ISSN: ESSN 1521-3773
PubMed: 32356371
DOI: 10.1002/ANIE.202004733