Copper in PDB 6jua: Aspergillus Oryzae Pro-Tyrosinase Oxygen-Bound C92A Mutant

All present enzymatic activity of Aspergillus Oryzae Pro-Tyrosinase Oxygen-Bound C92A Mutant:
1.14.18.1;

The structure of Aspergillus Oryzae Pro-Tyrosinase Oxygen-Bound C92A Mutant, PDB code: 6jua was solved by N.Fujieda, K.Umakoshi, Y.Nishikawa, G.Kurisu, S.Itoh, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.00 / 1.45
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	54.722, 118.811, 79.778, 90.00, 90.65, 90.00
R / Rfree (%)	18.7 / 24.6

The binding sites of Copper atom in the Aspergillus Oryzae Pro-Tyrosinase Oxygen-Bound C92A Mutant (pdb code 6jua). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 4 binding sites of Copper where determined in the Aspergillus Oryzae Pro-Tyrosinase Oxygen-Bound C92A Mutant, PDB code: 6jua:
Jump to Copper binding site number: 1; 2; 3; 4;

Copper binding site 1 out of 4 in the Aspergillus Oryzae Pro-Tyrosinase Oxygen-Bound C92A Mutant


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of Aspergillus Oryzae Pro-Tyrosinase Oxygen-Bound C92A Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu701 b:21.6 occ:0.78 NE2 A:HIS67 2.0 18.6 1.0 NE2 A:HIS94 2.2 19.1 1.0 O A:HOH1053 2.2 26.2 1.0 NE2 A:HIS103 2.2 17.2 1.0 CD2 A:HIS67 3.0 15.6 1.0 CE1 A:HIS67 3.0 17.8 1.0 CE1 A:HIS103 3.1 18.1 1.0 CE1 A:HIS94 3.1 19.8 1.0 CD2 A:HIS94 3.2 20.7 1.0 CD2 A:HIS103 3.3 16.7 1.0 CU A:CU702 3.7 19.4 0.8 ND1 A:HIS67 4.1 15.7 1.0 CG A:HIS67 4.1 16.1 1.0 CB A:ALA92 4.2 20.1 1.0 ND1 A:HIS103 4.2 17.2 1.0 ND1 A:HIS94 4.2 20.9 1.0 NE2 A:HIS372 4.2 17.6 1.0 CG A:HIS94 4.3 20.2 1.0 CZ A:PHE368 4.4 14.6 1.0 CG A:HIS103 4.4 14.1 1.0 CE1 A:HIS372 4.5 18.0 1.0 CE2 A:PHE368 4.5 15.8 1.0 CE2 A:PHE513 4.7 18.4 1.0 NE2 A:HIS328 4.7 18.2 1.0 CE1 A:PHE99 4.9 15.2 1.0 CZ3 A:TRP102 5.0 15.8 1.0

Copper binding site 2 out of 4 in the Aspergillus Oryzae Pro-Tyrosinase Oxygen-Bound C92A Mutant


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of Aspergillus Oryzae Pro-Tyrosinase Oxygen-Bound C92A Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu702 b:19.4 occ:0.76 O A:HOH1053 1.7 26.2 1.0 NE2 A:HIS328 1.9 18.2 1.0 NE2 A:HIS332 2.1 16.2 1.0 NE2 A:HIS372 2.3 17.6 1.0 CD2 A:HIS328 2.8 16.1 1.0 CD2 A:HIS332 3.0 15.2 1.0 CE1 A:HIS328 3.0 18.3 1.0 CE1 A:HIS372 3.1 18.0 1.0 CE1 A:HIS332 3.1 18.6 1.0 CD2 A:HIS372 3.4 16.8 1.0 CU A:CU701 3.7 21.6 0.8 CE2 A:PHE513 3.9 18.4 1.0 CG A:HIS328 4.0 17.2 1.0 CE2 A:PHE368 4.0 15.8 1.0 ND1 A:HIS328 4.0 18.0 1.0 ND1 A:HIS332 4.2 15.7 1.0 CG A:HIS332 4.2 15.1 1.0 ND1 A:HIS372 4.3 16.8 1.0 CZ A:PHE513 4.3 18.7 1.0 CG A:HIS372 4.4 15.1 1.0 CZ A:PHE368 4.6 14.6 1.0 NE2 A:HIS103 4.6 17.2 1.0 CD2 A:HIS371 4.6 16.6 1.0 CD2 A:PHE368 4.8 13.4 1.0 CE1 A:PHE99 4.8 15.2 1.0 CD2 A:PHE513 4.8 15.6 1.0 NE2 A:HIS67 4.8 18.6 1.0 CD2 A:HIS103 4.8 16.7 1.0 NE2 A:HIS371 4.8 16.8 1.0 NE2 A:HIS94 4.9 19.1 1.0

Copper binding site 3 out of 4 in the Aspergillus Oryzae Pro-Tyrosinase Oxygen-Bound C92A Mutant


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 3 of Aspergillus Oryzae Pro-Tyrosinase Oxygen-Bound C92A Mutant within 5.0Å range: probe atom residue distance (Å) B Occ B:Cu701 b:17.6 occ:0.75 O1 B:PER703 1.9 18.6 0.7 O2 B:PER703 2.0 18.7 0.7 NE2 B:HIS67 2.0 18.2 1.0 NE2 B:HIS94 2.1 17.4 1.0 NE2 B:HIS103 2.3 16.5 1.0 CE1 B:HIS67 2.9 16.6 1.0 CD2 B:HIS67 3.1 19.2 1.0 CE1 B:HIS103 3.1 18.0 1.0 CE1 B:HIS94 3.1 18.3 1.0 CD2 B:HIS94 3.2 18.8 1.0 CD2 B:HIS103 3.4 15.9 1.0 CU B:CU702 3.5 16.9 0.8 ND1 B:HIS67 4.1 14.9 1.0 CG B:HIS67 4.2 13.8 1.0 NE2 B:HIS372 4.2 16.3 1.0 ND1 B:HIS94 4.2 18.5 1.0 ND1 B:HIS103 4.3 17.7 1.0 CG B:HIS94 4.3 17.2 1.0 CZ B:PHE368 4.4 16.4 1.0 CG B:HIS103 4.4 16.4 1.0 CE1 B:HIS372 4.5 15.5 1.0 CE2 B:PHE368 4.5 14.2 1.0 CB B:ALA92 4.5 21.8 1.0 CE2 B:PHE513 4.6 18.7 1.0 NE2 B:HIS328 4.7 17.3 1.0 CE1 B:PHE99 4.8 14.4 1.0 CG1 B:VAL359 4.9 29.2 1.0

Copper binding site 4 out of 4 in the Aspergillus Oryzae Pro-Tyrosinase Oxygen-Bound C92A Mutant


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 4 of Aspergillus Oryzae Pro-Tyrosinase Oxygen-Bound C92A Mutant within 5.0Å range: probe atom residue distance (Å) B Occ B:Cu702 b:16.9 occ:0.78 O2 B:PER703 1.9 18.7 0.7 NE2 B:HIS328 1.9 17.3 1.0 O1 B:PER703 1.9 18.6 0.7 NE2 B:HIS332 2.1 14.8 1.0 NE2 B:HIS372 2.2 16.3 1.0 CD2 B:HIS328 2.9 14.7 1.0 CE1 B:HIS328 2.9 19.7 1.0 CE1 B:HIS332 3.0 15.0 1.0 CE1 B:HIS372 3.0 15.5 1.0 CD2 B:HIS332 3.0 15.3 1.0 CD2 B:HIS372 3.3 17.9 1.0 CU B:CU701 3.5 17.6 0.8 CE2 B:PHE513 3.8 18.7 1.0 ND1 B:HIS328 4.0 17.7 1.0 CG B:HIS328 4.0 17.6 1.0 ND1 B:HIS332 4.1 14.5 1.0 CZ B:PHE513 4.1 15.5 1.0 CE2 B:PHE368 4.1 14.2 1.0 CG B:HIS332 4.2 14.8 1.0 ND1 B:HIS372 4.2 16.0 1.0 CG B:HIS372 4.4 14.3 1.0 NE2 B:HIS103 4.5 16.5 1.0 CZ B:PHE368 4.7 16.4 1.0 CE1 B:PHE99 4.7 14.4 1.0 CD2 B:PHE513 4.7 15.6 1.0 CD2 B:HIS371 4.8 14.9 1.0 CD2 B:HIS103 4.8 15.9 1.0 CD2 B:PHE368 4.8 13.7 1.0 NE2 B:HIS94 4.9 17.4 1.0 NE2 B:HIS67 4.9 18.2 1.0

N.Fujieda, K.Umakoshi, Y.Ochi, Y.Nishikawa, S.Yanagisawa, M.Kubo, G.Kurisu, S.Itoh. Copper-Oxygen Dynamics in Tyrosinase Mechanism. Angew.Chem.Int.Ed.Engl. 2020.
ISSN: ESSN 1521-3773
PubMed: 32356371
DOI: 10.1002/ANIE.202004733