Copper in PDB 5z22: Crystal Structure of Laccase From Cerrena Sp. RSD1

All present enzymatic activity of Crystal Structure of Laccase From Cerrena Sp. RSD1:
1.10.3.2;

The structure of Crystal Structure of Laccase From Cerrena Sp. RSD1, PDB code: 5z22 was solved by C.C.Lee, M.H.Wu, T.H.Ho, A.H.J.Wang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.00 / 1.50
Space group	P 43 21 2
Cell size a, b, c (Å), α, β, γ (°)	87.409, 87.409, 148.791, 90.00, 90.00, 90.00
R / Rfree (%)	12.6 / 15.2

The binding sites of Copper atom in the Crystal Structure of Laccase From Cerrena Sp. RSD1 (pdb code 5z22). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 4 binding sites of Copper where determined in the Crystal Structure of Laccase From Cerrena Sp. RSD1, PDB code: 5z22:
Jump to Copper binding site number: 1; 2; 3; 4;

Copper binding site 1 out of 4 in the Crystal Structure of Laccase From Cerrena Sp. RSD1


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of Crystal Structure of Laccase From Cerrena Sp. RSD1 within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu508 b:15.7 occ:1.00 ND1 A:HIS454 2.1 15.2 1.0 ND1 A:HIS393 2.1 16.1 1.0 SG A:CYS449 2.2 15.5 1.0 CE1 A:HIS393 3.0 17.1 1.0 CE1 A:HIS454 3.0 16.1 1.0 CG A:HIS454 3.1 15.3 1.0 CG A:HIS393 3.1 15.7 1.0 CB A:CYS449 3.3 15.8 1.0 CB A:HIS454 3.4 15.1 1.0 CD1 A:ILE451 3.5 15.7 1.0 CB A:HIS393 3.5 15.0 1.0 CB A:ILE451 3.7 14.1 1.0 CD2 A:PHE459 3.7 16.3 1.0 CE2 A:PHE459 4.0 16.8 1.0 CG1 A:ILE451 4.0 15.4 1.0 CA A:HIS393 4.0 14.4 1.0 NE2 A:HIS393 4.1 17.8 1.0 NE2 A:HIS454 4.2 16.9 1.0 CD2 A:HIS454 4.2 15.8 1.0 CD2 A:HIS393 4.2 17.2 1.0 CG2 A:ILE451 4.6 13.8 1.0 CA A:CYS449 4.6 14.9 1.0 CD A:PRO394 4.7 14.4 1.0 N A:ILE451 4.7 13.9 1.0 O A:GLY390 4.7 23.6 1.0 CA A:ILE451 4.8 14.1 1.0 O A:ILE451 4.8 14.6 1.0 CA A:HIS454 4.9 15.0 1.0 C A:HIS393 5.0 13.9 1.0

Copper binding site 2 out of 4 in the Crystal Structure of Laccase From Cerrena Sp. RSD1


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of Crystal Structure of Laccase From Cerrena Sp. RSD1 within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu509 b:17.3 occ:1.00 NE2 A:HIS396 1.9 13.9 1.0 NE2 A:HIS64 1.9 13.0 1.0 O A:HOH889 2.5 15.4 1.0 CD2 A:HIS396 2.9 14.4 1.0 CE1 A:HIS64 2.9 13.4 1.0 CE1 A:HIS396 2.9 14.7 1.0 CD2 A:HIS64 2.9 14.2 1.0 O2 A:OXY512 3.2 40.0 1.0 NE2 A:HIS398 3.4 14.2 1.0 ND1 A:HIS66 3.5 13.8 1.0 CE1 A:HIS398 3.5 13.8 1.0 CD2 A:HIS398 3.6 13.3 1.0 CG A:HIS66 3.7 13.0 1.0 ND1 A:HIS398 3.7 14.5 1.0 CG A:HIS398 3.8 14.0 1.0 CE1 A:HIS66 3.9 13.9 1.0 CA A:HIS66 3.9 12.6 1.0 O1 A:OXY512 3.9 31.6 1.0 ND1 A:HIS64 4.0 13.8 1.0 ND1 A:HIS396 4.0 14.6 1.0 CG A:HIS396 4.0 13.6 1.0 CG A:HIS64 4.0 13.0 1.0 N A:GLY67 4.1 13.7 1.0 CU A:CU510 4.1 15.1 1.0 CB A:HIS66 4.1 12.7 1.0 CD2 A:HIS66 4.2 13.4 1.0 NE2 A:HIS66 4.3 13.5 1.0 CU A:CU511 4.5 18.3 1.0 C A:HIS66 4.5 13.4 1.0 O A:HOH754 4.6 16.3 1.0 O A:HOH712 4.6 15.1 1.0 CA A:HIS398 4.6 14.2 1.0 CB A:HIS398 4.7 13.9 1.0 N A:HIS66 4.9 11.9 1.0

Copper binding site 3 out of 4 in the Crystal Structure of Laccase From Cerrena Sp. RSD1


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 3 of Crystal Structure of Laccase From Cerrena Sp. RSD1 within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu510 b:15.1 occ:1.00 O2 A:OXY512 2.0 40.0 1.0 ND1 A:HIS66 2.0 13.8 1.0 NE2 A:HIS109 2.1 13.8 1.0 NE2 A:HIS450 2.2 13.9 1.0 CE1 A:HIS66 2.9 13.9 1.0 CE1 A:HIS450 3.0 13.1 1.0 CD2 A:HIS109 3.0 12.9 1.0 O1 A:OXY512 3.0 31.6 1.0 CG A:HIS66 3.1 13.0 1.0 CE1 A:HIS109 3.1 13.0 1.0 CD2 A:HIS450 3.3 14.0 1.0 CB A:HIS66 3.4 12.7 1.0 CZ2 A:TRP107 3.4 12.9 1.0 CE2 A:TRP107 3.8 12.6 1.0 CD2 A:HIS64 3.9 14.2 1.0 NE1 A:TRP107 4.0 12.8 1.0 CU A:CU509 4.1 17.3 1.0 NE2 A:HIS66 4.1 13.5 1.0 ND1 A:HIS109 4.2 13.6 1.0 CD2 A:HIS66 4.2 13.4 1.0 CH2 A:TRP107 4.2 12.7 1.0 CG A:HIS109 4.2 12.6 1.0 ND1 A:HIS450 4.2 13.6 1.0 CG A:HIS450 4.3 13.7 1.0 NE2 A:HIS64 4.4 13.0 1.0 CD2 A:HIS396 4.4 14.4 1.0 NE2 A:HIS396 4.5 13.9 1.0 O A:HOH694 4.5 21.5 1.0 CA A:HIS66 4.6 12.6 1.0 CD2 A:TRP107 4.7 11.9 1.0 CD1 A:TRP107 4.9 12.4 1.0

Copper binding site 4 out of 4 in the Crystal Structure of Laccase From Cerrena Sp. RSD1


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 4 of Crystal Structure of Laccase From Cerrena Sp. RSD1 within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu511 b:18.3 occ:1.00 NE2 A:HIS448 2.0 15.8 1.0 NE2 A:HIS398 2.0 14.2 1.0 NE2 A:HIS111 2.1 13.9 1.0 O1 A:OXY512 2.2 31.6 1.0 CE1 A:HIS398 2.9 13.8 1.0 CD2 A:HIS448 3.0 15.6 1.0 CE1 A:HIS448 3.0 16.3 1.0 CE1 A:HIS111 3.1 15.5 1.0 CD2 A:HIS398 3.1 13.3 1.0 CD2 A:HIS111 3.2 13.4 1.0 O2 A:OXY512 3.2 40.0 1.0 O A:HOH694 3.7 21.5 1.0 CD2 A:HIS396 4.0 14.4 1.0 ND1 A:HIS398 4.1 14.5 1.0 ND1 A:HIS448 4.1 15.1 1.0 CG A:HIS448 4.1 15.2 1.0 CD2 A:PHE446 4.2 16.2 1.0 CG A:HIS398 4.2 14.0 1.0 ND1 A:HIS111 4.2 15.1 1.0 CG A:HIS111 4.3 13.6 1.0 CU A:CU509 4.5 17.3 1.0 CB A:PHE446 4.5 15.2 1.0 CD2 A:HIS64 4.6 14.2 1.0 CG A:PRO79 4.6 14.3 1.0 NE2 A:HIS396 4.7 13.9 1.0 NE2 A:HIS64 4.7 13.0 1.0 CG A:PHE446 4.8 14.9 1.0

M.H.Wu, M.C.Lin, C.C.Lee, A.H.J.Wang, T.H.Ho. Effect of Organic Solvents on Activity and Stability of Laccases To Be Published.