Copper in PDB 5xmo: X-Ray Crystal Structure of Pseudoazurin MET16PHE/THR36LYS Variant

The structure of X-Ray Crystal Structure of Pseudoazurin MET16PHE/THR36LYS Variant, PDB code: 5xmo was solved by T.Yamaguchi, T.Kohzuma, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	46.05 / 1.19
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	93.170, 41.160, 34.830, 90.00, 98.71, 90.00
R / Rfree (%)	12.8 / 16.1

The binding sites of Copper atom in the X-Ray Crystal Structure of Pseudoazurin MET16PHE/THR36LYS Variant (pdb code 5xmo). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 2 binding sites of Copper where determined in the X-Ray Crystal Structure of Pseudoazurin MET16PHE/THR36LYS Variant, PDB code: 5xmo:
Jump to Copper binding site number: 1; 2;

Copper binding site 1 out of 2 in the X-Ray Crystal Structure of Pseudoazurin MET16PHE/THR36LYS Variant


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of X-Ray Crystal Structure of Pseudoazurin MET16PHE/THR36LYS Variant within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu201 b:9.5 occ:0.50 CU A:CU201 0.0 9.5 0.5 CU A:CU201 0.5 6.3 0.5 ND1 A:HIS81 2.0 8.9 1.0 ND1 A:HIS40 2.0 7.8 1.0 SG A:CYS78 2.1 9.1 1.0 CE1 A:HIS81 2.9 9.0 1.0 SD A:MET86 3.0 6.4 1.0 CE1 A:HIS40 3.0 8.1 1.0 CG A:HIS81 3.1 7.9 1.0 CG A:HIS40 3.1 7.8 1.0 CB A:CYS78 3.2 6.8 1.0 CB A:HIS40 3.5 7.5 1.0 CB A:HIS81 3.5 7.5 1.0 CA A:HIS40 3.5 8.1 1.0 O A:GLY39 3.5 11.9 1.0 CE A:MET86 3.7 7.6 1.0 CG A:PRO80 4.0 11.7 1.0 NE2 A:HIS81 4.1 9.7 1.0 C A:GLY39 4.2 9.9 1.0 NE2 A:HIS40 4.2 8.3 1.0 CD2 A:HIS81 4.2 9.8 1.0 N A:HIS81 4.2 7.5 1.0 CD2 A:HIS40 4.2 10.0 1.0 N A:HIS40 4.3 9.2 1.0 CG A:MET86 4.4 5.8 1.0 CA A:HIS81 4.5 7.1 1.0 CA A:CYS78 4.5 5.9 1.0 N A:ASN41 4.6 9.2 1.0 CD A:PRO80 4.7 9.2 1.0 C A:HIS40 4.7 9.6 1.0 CB A:MET86 4.8 5.1 1.0 C A:CYS78 4.9 6.4 1.0 O A:CYS78 5.0 8.3 1.0 CB A:PRO80 5.0 10.6 1.0

Copper binding site 2 out of 2 in the X-Ray Crystal Structure of Pseudoazurin MET16PHE/THR36LYS Variant


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of X-Ray Crystal Structure of Pseudoazurin MET16PHE/THR36LYS Variant within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu201 b:6.3 occ:0.50 CU A:CU201 0.0 6.3 0.5 CU A:CU201 0.5 9.5 0.5 ND1 A:HIS40 2.0 7.8 1.0 ND1 A:HIS81 2.1 8.9 1.0 SG A:CYS78 2.3 9.1 1.0 SD A:MET86 2.5 6.4 1.0 CE1 A:HIS40 2.9 8.1 1.0 CE1 A:HIS81 3.1 9.0 1.0 CG A:HIS81 3.1 7.9 1.0 CG A:HIS40 3.1 7.8 1.0 CB A:CYS78 3.2 6.8 1.0 CE A:MET86 3.3 7.6 1.0 CB A:HIS81 3.4 7.5 1.0 CB A:HIS40 3.5 7.5 1.0 CA A:HIS40 3.8 8.1 1.0 O A:GLY39 3.9 11.9 1.0 CG A:MET86 4.0 5.8 1.0 NE2 A:HIS40 4.1 8.3 1.0 CD2 A:HIS40 4.2 10.0 1.0 CD2 A:HIS81 4.2 9.8 1.0 NE2 A:HIS81 4.2 9.7 1.0 N A:HIS81 4.3 7.5 1.0 CB A:MET86 4.4 5.1 1.0 CG A:PRO80 4.5 11.7 1.0 CA A:HIS81 4.5 7.1 1.0 C A:GLY39 4.5 9.9 1.0 N A:HIS40 4.6 9.2 1.0 CA A:CYS78 4.6 5.9 1.0 CD1 A:PHE16 4.8 9.7 1.0 N A:ASN41 4.9 9.2 1.0 C A:HIS40 4.9 9.6 1.0 C A:CYS78 5.0 6.4 1.0

T.Yamaguchi, N.Takebayashi, K.Akao, C.Sakai, T.Kohzuma. X-Ray Crystallographic Analysis of M16F/T36K Double Mutant of Pseudoazurin Photon Factory Activity V. 34 2017REPORT.
ISSN: ISSN 0912-1803