Atomistry » Copper » PDB 4tm7-4yst » 4ysc
Atomistry »
  Copper »
    PDB 4tm7-4yst »
      4ysc »

Copper in PDB 4ysc: Completely Oxidized Structure of Copper Nitrite Reductase From Alcaligenes Faecalis

Enzymatic activity of Completely Oxidized Structure of Copper Nitrite Reductase From Alcaligenes Faecalis

All present enzymatic activity of Completely Oxidized Structure of Copper Nitrite Reductase From Alcaligenes Faecalis:
1.7.2.1;

Protein crystallography data

The structure of Completely Oxidized Structure of Copper Nitrite Reductase From Alcaligenes Faecalis, PDB code: 4ysc was solved by Y.Fukuda, K.M.Tse, M.Suzuki, K.Diederichs, K.Hirata, T.Nakane, M.Sugahara, E.Nango, K.Tono, Y.Joti, T.Kameshima, C.Song, T.Hatsui, M.Yabashi, O.Nureki, H.Matsumura, T.Inoue, S.Iwata, E.Mizohata, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 73.90 / 2.03
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 63.100, 103.800, 147.800, 90.00, 90.00, 90.00
R / Rfree (%) 16.4 / 20.3

Other elements in 4ysc:

The structure of Completely Oxidized Structure of Copper Nitrite Reductase From Alcaligenes Faecalis also contains other interesting chemical elements:

Chlorine (Cl) 3 atoms

Copper Binding Sites:

The binding sites of Copper atom in the Completely Oxidized Structure of Copper Nitrite Reductase From Alcaligenes Faecalis (pdb code 4ysc). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 6 binding sites of Copper where determined in the Completely Oxidized Structure of Copper Nitrite Reductase From Alcaligenes Faecalis, PDB code: 4ysc:
Jump to Copper binding site number: 1; 2; 3; 4; 5; 6;

Copper binding site 1 out of 6 in 4ysc

Go back to Copper Binding Sites List in 4ysc
Copper binding site 1 out of 6 in the Completely Oxidized Structure of Copper Nitrite Reductase From Alcaligenes Faecalis


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of Completely Oxidized Structure of Copper Nitrite Reductase From Alcaligenes Faecalis within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cu401

b:35.2
occ:1.00
 ND1 A:HIS145 2.0 30.6 1.0
SG A:CYS136 2.2 31.0 1.0
ND1 A:HIS95 2.2 29.4 1.0
SD A:MET150 2.5 30.6 1.0
CE1 A:HIS145 2.9 35.4 1.0
CG A:HIS145 3.1 31.9 1.0
CG A:HIS95 3.1 27.9 1.0
CB A:CYS136 3.2 31.0 1.0
CE1 A:HIS95 3.2 30.9 1.0
CE A:MET150 3.3 27.1 1.0
CB A:HIS95 3.4 28.5 1.0
CB A:HIS145 3.5 28.8 1.0
CA A:HIS95 3.8 32.1 1.0
CG A:MET150 3.9 28.9 1.0
NE2 A:HIS145 4.1 35.8 1.0
CD2 A:HIS145 4.2 32.1 1.0
O A:MET94 4.3 29.6 1.0
CD2 A:HIS95 4.3 28.6 1.0
NE2 A:HIS95 4.3 33.6 1.0
CG A:PRO138 4.3 32.4 1.0
CB A:MET150 4.4 28.2 1.0
SD A:MET62 4.4 32.5 1.0
CA A:CYS136 4.7 29.3 1.0
CD A:PRO138 4.7 29.6 1.0
CA A:HIS145 4.7 29.4 1.0
N A:ASN96 4.7 32.2 1.0
CB A:MET62 4.8 31.7 1.0
C A:HIS95 4.9 31.1 1.0
N A:HIS95 4.9 30.1 1.0
C A:MET94 5.0 31.9 1.0

Copper binding site 2 out of 6 in 4ysc

Go back to Copper Binding Sites List in 4ysc
Copper binding site 2 out of 6 in the Completely Oxidized Structure of Copper Nitrite Reductase From Alcaligenes Faecalis


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of Completely Oxidized Structure of Copper Nitrite Reductase From Alcaligenes Faecalis within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cu402

b:33.7
occ:1.00
 NE2 C:HIS306 2.0 30.6 1.0
NE2 A:HIS100 2.1 25.3 1.0
NE2 A:HIS135 2.1 30.3 1.0
CL A:CL403 2.2 38.1 1.0
CE1 C:HIS306 3.0 28.3 1.0
CD2 A:HIS135 3.0 27.9 1.0
CE1 A:HIS100 3.0 27.4 1.0
CD2 C:HIS306 3.0 27.2 1.0
CD2 A:HIS100 3.1 25.0 1.0
CE1 A:HIS135 3.2 27.8 1.0
NE2 C:HIS255 3.9 33.8 1.0
OD2 A:ASP98 3.9 37.0 1.0
CD2 C:HIS255 4.0 30.0 1.0
ND1 C:HIS306 4.1 26.9 1.0
CG C:HIS306 4.1 31.9 1.0
ND1 A:HIS100 4.1 26.4 1.0
CG A:HIS135 4.2 28.5 1.0
CG A:HIS100 4.2 27.4 1.0
ND1 A:HIS135 4.3 28.2 1.0
CE1 C:HIS255 4.6 32.1 1.0
CG A:ASP98 4.7 32.5 1.0
CG C:HIS255 4.7 28.2 1.0
ND1 C:HIS255 5.0 32.4 1.0
CD1 C:LEU308 5.0 27.8 1.0
CD2 C:LEU308 5.0 28.9 1.0

Copper binding site 3 out of 6 in 4ysc

Go back to Copper Binding Sites List in 4ysc
Copper binding site 3 out of 6 in the Completely Oxidized Structure of Copper Nitrite Reductase From Alcaligenes Faecalis


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 3 of Completely Oxidized Structure of Copper Nitrite Reductase From Alcaligenes Faecalis within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Cu401

b:37.0
occ:1.00
 ND1 B:HIS145 2.0 33.5 1.0
ND1 B:HIS95 2.1 32.5 1.0
SG B:CYS136 2.1 31.1 1.0
SD B:MET150 2.6 33.3 1.0
CE1 B:HIS145 2.9 35.4 1.0
CG B:HIS95 3.0 31.8 1.0
CG B:HIS145 3.1 32.7 1.0
CE1 B:HIS95 3.2 32.4 1.0
CB B:HIS95 3.2 31.6 1.0
CB B:CYS136 3.3 29.3 1.0
CB B:HIS145 3.5 29.1 1.0
CE B:MET150 3.5 33.6 1.0
CA B:HIS95 3.7 32.6 1.0
CG B:MET150 4.0 32.0 1.0
NE2 B:HIS145 4.1 38.2 1.0
CD2 B:HIS95 4.2 33.2 1.0
CD2 B:HIS145 4.2 32.6 1.0
O B:MET94 4.2 33.2 1.0
NE2 B:HIS95 4.2 33.2 1.0
CG B:PRO138 4.3 40.1 1.0
SD B:MET62 4.4 32.6 1.0
CB B:MET150 4.5 28.8 1.0
N B:ASN96 4.6 36.4 1.0
CA B:CYS136 4.7 33.6 1.0
C B:HIS95 4.7 35.6 1.0
CD B:PRO138 4.7 35.9 1.0
CA B:HIS145 4.7 30.1 1.0
N B:HIS95 4.8 35.2 1.0
CB B:MET62 4.9 26.8 1.0
C B:MET94 4.9 37.6 1.0

Copper binding site 4 out of 6 in 4ysc

Go back to Copper Binding Sites List in 4ysc
Copper binding site 4 out of 6 in the Completely Oxidized Structure of Copper Nitrite Reductase From Alcaligenes Faecalis


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 4 of Completely Oxidized Structure of Copper Nitrite Reductase From Alcaligenes Faecalis within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Cu402

b:34.8
occ:1.00
 NE2 A:HIS306 2.0 29.1 1.0
NE2 B:HIS100 2.1 29.4 1.0
NE2 B:HIS135 2.2 30.5 1.0
CL A:CL404 2.2 40.8 1.0
CE1 A:HIS306 3.0 26.2 1.0
CE1 B:HIS100 3.0 31.4 1.0
CD2 B:HIS135 3.1 28.8 1.0
CD2 A:HIS306 3.1 27.0 1.0
CD2 B:HIS100 3.2 28.9 1.0
CE1 B:HIS135 3.2 28.1 1.0
NE2 A:HIS255 3.8 29.4 1.0
OD2 B:ASP98 3.8 39.5 1.0
CD2 A:HIS255 3.9 27.6 1.0
ND1 A:HIS306 4.1 27.9 1.0
ND1 B:HIS100 4.2 29.9 1.0
CG A:HIS306 4.2 28.6 1.0
CG B:HIS135 4.2 28.1 1.0
CG B:HIS100 4.3 30.2 1.0
ND1 B:HIS135 4.3 28.8 1.0
CE1 A:HIS255 4.5 30.8 1.0
CG B:ASP98 4.5 33.4 1.0
CG A:HIS255 4.7 28.1 1.0
O B:HOH579 4.9 36.2 1.0
OD1 B:ASP98 4.9 36.8 1.0
CD2 A:LEU308 4.9 32.2 1.0
ND1 A:HIS255 4.9 30.5 1.0

Copper binding site 5 out of 6 in 4ysc

Go back to Copper Binding Sites List in 4ysc
Copper binding site 5 out of 6 in the Completely Oxidized Structure of Copper Nitrite Reductase From Alcaligenes Faecalis


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 5 of Completely Oxidized Structure of Copper Nitrite Reductase From Alcaligenes Faecalis within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Cu401

b:29.7
occ:1.00
 ND1 C:HIS145 2.0 24.8 1.0
ND1 C:HIS95 2.0 26.7 1.0
SG C:CYS136 2.2 25.3 1.0
SD C:MET150 2.5 27.7 1.0
CE1 C:HIS145 2.9 26.2 1.0
CE1 C:HIS95 2.9 25.2 1.0
CG C:HIS145 3.0 26.2 1.0
CG C:HIS95 3.1 29.0 1.0
CB C:CYS136 3.2 23.6 1.0
CE C:MET150 3.4 24.0 1.0
CB C:HIS145 3.4 25.7 1.0
CB C:HIS95 3.4 30.4 1.0
CA C:HIS95 3.8 29.0 1.0
CG C:MET150 4.0 27.1 1.0
NE2 C:HIS145 4.1 26.5 1.0
NE2 C:HIS95 4.1 29.0 1.0
CD2 C:HIS145 4.1 27.8 1.0
CD2 C:HIS95 4.2 26.2 1.0
CG C:PRO138 4.3 26.2 1.0
O C:MET94 4.3 29.5 1.0
CB C:MET150 4.4 21.7 1.0
SD C:MET62 4.5 30.8 1.0
N C:ASN96 4.6 29.6 1.0
CA C:CYS136 4.6 24.0 1.0
CA C:HIS145 4.7 27.4 1.0
CD C:PRO138 4.8 27.3 1.0
C C:HIS95 4.8 30.3 1.0
CB C:MET62 4.9 24.6 1.0
N C:HIS95 4.9 28.4 1.0

Copper binding site 6 out of 6 in 4ysc

Go back to Copper Binding Sites List in 4ysc
Copper binding site 6 out of 6 in the Completely Oxidized Structure of Copper Nitrite Reductase From Alcaligenes Faecalis


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 6 of Completely Oxidized Structure of Copper Nitrite Reductase From Alcaligenes Faecalis within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Cu402

b:29.6
occ:1.00
 NE2 C:HIS100 2.0 22.2 1.0
NE2 B:HIS306 2.0 21.3 1.0
NE2 C:HIS135 2.0 25.8 1.0
CL B:CL403 2.2 35.6 1.0
CE1 C:HIS100 2.9 22.2 1.0
CE1 B:HIS306 3.0 20.6 1.0
CE1 C:HIS135 3.0 27.3 1.0
CD2 B:HIS306 3.0 24.6 1.0
CD2 C:HIS135 3.0 28.2 1.0
CD2 C:HIS100 3.1 21.4 1.0
OD2 C:ASP98 4.0 33.6 1.0
ND1 C:HIS100 4.0 23.1 1.0
NE2 B:HIS255 4.0 28.7 1.0
ND1 B:HIS306 4.1 23.5 1.0
ND1 C:HIS135 4.1 27.1 1.0
CG C:HIS100 4.2 22.5 1.0
CG C:HIS135 4.2 26.5 1.0
CG B:HIS306 4.2 23.5 1.0
CD2 B:HIS255 4.2 27.6 1.0
CE1 B:HIS255 4.6 28.8 1.0
CG C:ASP98 4.7 30.4 1.0
CG B:HIS255 4.8 25.4 1.0
O C:HOH520 4.8 28.5 1.0
CD2 B:LEU308 4.9 25.3 1.0
OD1 C:ASP98 4.9 28.8 1.0
ND1 B:HIS255 5.0 29.9 1.0

Reference:

Y.Fukuda, K.M.Tse, T.Nakane, T.Nakatsu, M.Suzuki, M.Sugahara, S.Inoue, T.Masuda, F.Yumoto, N.Matsugaki, E.Nango, K.Tono, Y.Joti, T.Kameshima, C.Song, T.Hatsui, M.Yabashi, O.Nureki, M.E.Murphy, T.Inoue, S.Iwata, E.Mizohata. Redox-Coupled Proton Transfer Mechanism in Nitrite Reductase Revealed By Femtosecond Crystallography Proc.Natl.Acad.Sci.Usa V. 113 2928 2016.
ISSN: ESSN 1091-6490
PubMed: 26929369
DOI: 10.1073/PNAS.1517770113
Page generated: Mon Jul 14 04:10:42 2025

Last articles

Fe in 2YXO
Fe in 2YRS
Fe in 2YXC
Fe in 2YNM
Fe in 2YVJ
Fe in 2YP1
Fe in 2YU2
Fe in 2YU1
Fe in 2YQB
Fe in 2YOO
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy