Copper in PDB 4j6v: Crystal Structure of Tyrosinase From Bacillus Megaterium N205D Mutant

All present enzymatic activity of Crystal Structure of Tyrosinase From Bacillus Megaterium N205D Mutant:
1.14.18.1;

The structure of Crystal Structure of Tyrosinase From Bacillus Megaterium N205D Mutant, PDB code: 4j6v was solved by M.Kanteev, M.Goldfeder, N.Adir, A.Fishman, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	39.39 / 1.90
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	54.900, 78.330, 82.160, 90.00, 105.50, 90.00
R / Rfree (%)	19.3 / 21

The binding sites of Copper atom in the Crystal Structure of Tyrosinase From Bacillus Megaterium N205D Mutant (pdb code 4j6v). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 2 binding sites of Copper where determined in the Crystal Structure of Tyrosinase From Bacillus Megaterium N205D Mutant, PDB code: 4j6v:
Jump to Copper binding site number: 1; 2;

Copper binding site 1 out of 2 in the Crystal Structure of Tyrosinase From Bacillus Megaterium N205D Mutant


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of Crystal Structure of Tyrosinase From Bacillus Megaterium N205D Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu401 b:27.2 occ:0.36 NE2 A:HIS42 1.9 21.6 1.0 O A:HOH509 1.9 16.4 1.0 NE2 A:HIS60 2.2 22.4 1.0 NE2 A:HIS69 2.4 11.4 1.0 CE1 A:HIS42 2.7 19.7 1.0 CE1 A:HIS60 3.0 16.0 1.0 CD2 A:HIS42 3.1 17.1 1.0 CE1 A:HIS69 3.2 14.1 1.0 CD2 A:HIS60 3.3 16.6 1.0 CD2 A:HIS69 3.3 10.8 1.0 CZ A:PHE227 3.8 13.7 1.0 ND1 A:HIS42 3.9 20.9 1.0 NE2 A:HIS231 3.9 15.7 1.0 CG A:HIS42 4.1 16.9 1.0 ND1 A:HIS60 4.1 23.1 1.0 CE2 A:PHE227 4.2 14.4 1.0 CG1 A:VAL218 4.2 38.4 1.0 CE1 A:HIS231 4.3 14.8 1.0 CG A:HIS60 4.3 18.5 1.0 ND1 A:HIS69 4.4 9.9 1.0 CG A:HIS69 4.4 9.8 1.0 O A:HOH530 4.7 30.4 1.0 CE1 A:PHE65 4.9 14.4 1.0 CD2 A:HIS231 4.9 13.2 1.0 O A:ALA59 4.9 12.3 1.0

Copper binding site 2 out of 2 in the Crystal Structure of Tyrosinase From Bacillus Megaterium N205D Mutant


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of Crystal Structure of Tyrosinase From Bacillus Megaterium N205D Mutant within 5.0Å range: probe atom residue distance (Å) B Occ B:Cu401 b:33.0 occ:0.41 NE2 B:HIS42 1.9 17.2 1.0 NE2 B:HIS60 2.0 18.2 1.0 O B:HOH635 2.1 14.2 1.0 NE2 B:HIS69 2.6 11.6 1.0 CE1 B:HIS60 2.8 13.8 1.0 CE1 B:HIS42 2.8 16.5 1.0 CD2 B:HIS42 2.9 15.6 1.0 CD2 B:HIS60 3.1 14.4 1.0 CE1 B:HIS69 3.3 12.4 1.0 CD2 B:HIS69 3.7 10.8 1.0 CG1 B:VAL218 3.9 28.6 1.0 ND1 B:HIS60 3.9 17.4 1.0 CZ B:PHE227 3.9 11.1 1.0 ND1 B:HIS42 4.0 14.8 1.0 CG B:HIS42 4.1 13.6 1.0 CG B:HIS60 4.1 16.3 1.0 NE2 B:HIS231 4.1 15.2 1.0 CE2 B:PHE227 4.2 11.4 1.0 ND1 B:HIS69 4.5 10.1 1.0 CE1 B:HIS231 4.5 13.5 1.0 O B:HOH661 4.6 28.8 1.0 CG B:HIS69 4.7 8.9 1.0 O B:ALA59 4.9 11.7 1.0 O B:VAL218 5.0 26.6 1.0

M.Kanteev, M.Goldfeder, M.Chojnacki, N.Adir, A.Fishman. The Mechanism of Copper Uptake By Tyrosinase From Bacillus Megaterium. J.Biol.Inorg.Chem. V. 18 895 2013.
ISSN: ISSN 0949-8257
PubMed: 24061559
DOI: 10.1007/S00775-013-1034-0