Copper in PDB 4hd4: Crystal Structure of Tyrosinase From Bacillus Megaterium V218F Mutant

The structure of Crystal Structure of Tyrosinase From Bacillus Megaterium V218F Mutant, PDB code: 4hd4 was solved by M.Goldfeder, M.Kanteev, N.Adir, A.Fishman, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	28.45 / 1.80
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	48.000, 78.580, 85.890, 90.00, 106.13, 90.00
R / Rfree (%)	20.7 / 22.8

The binding sites of Copper atom in the Crystal Structure of Tyrosinase From Bacillus Megaterium V218F Mutant (pdb code 4hd4). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 2 binding sites of Copper where determined in the Crystal Structure of Tyrosinase From Bacillus Megaterium V218F Mutant, PDB code: 4hd4:
Jump to Copper binding site number: 1; 2;

Copper binding site 1 out of 2 in the Crystal Structure of Tyrosinase From Bacillus Megaterium V218F Mutant


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of Crystal Structure of Tyrosinase From Bacillus Megaterium V218F Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu401 b:36.3 occ:0.50 NE2 A:HIS60 2.0 22.1 0.2 O A:HOH619 2.1 30.0 1.0 NE2 A:HIS69 2.2 6.1 1.0 NE2 A:HIS42 2.2 11.4 1.0 CE1 A:HIS60 2.4 19.2 0.2 CE1 A:HIS69 2.9 7.2 1.0 CD2 A:HIS42 3.1 12.4 1.0 CE1 A:HIS42 3.3 12.4 1.0 CD2 A:HIS60 3.3 20.6 0.2 CD2 A:HIS69 3.4 8.7 1.0 CE1 A:PHE218 3.5 20.4 1.0 ND1 A:HIS60 3.6 18.1 0.2 NE2 A:HIS231 3.8 9.8 1.0 CD1 A:PHE218 3.9 23.9 1.0 CZ A:PHE227 3.9 7.0 1.0 CE1 A:HIS231 4.0 9.2 1.0 ND1 A:HIS69 4.1 6.3 1.0 CG A:HIS60 4.1 16.1 0.2 CE2 A:PHE227 4.2 6.2 1.0 CG A:HIS42 4.3 8.8 1.0 CG A:HIS69 4.3 6.1 1.0 ND1 A:HIS42 4.4 10.7 1.0 CZ A:PHE218 4.5 19.2 1.0 CE1 A:PHE65 4.8 7.3 1.0 NE2 A:HIS204 4.8 8.6 1.0 O A:ALA59 4.8 8.3 1.0 CD2 A:HIS231 4.9 9.1 1.0 CD2 A:HIS60 4.9 20.6 0.8

Copper binding site 2 out of 2 in the Crystal Structure of Tyrosinase From Bacillus Megaterium V218F Mutant


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of Crystal Structure of Tyrosinase From Bacillus Megaterium V218F Mutant within 5.0Å range: probe atom residue distance (Å) B Occ B:Cu401 b:34.2 occ:0.40 O B:HOH616 2.0 30.0 1.0 NE2 B:HIS42 2.2 9.3 1.0 NE2 B:HIS69 2.3 8.3 1.0 CE1 B:HIS69 2.8 9.6 1.0 CD2 B:HIS42 3.1 9.3 1.0 CE1 B:HIS42 3.3 12.4 1.0 CE1 B:PHE218 3.4 20.9 1.0 CD2 B:HIS69 3.5 6.5 1.0 CD1 B:PHE218 3.8 22.6 1.0 CZ B:PHE227 4.0 7.0 1.0 ND1 B:HIS69 4.1 6.8 1.0 NE2 B:HIS231 4.3 11.0 1.0 CG B:HIS42 4.3 11.9 1.0 CE2 B:PHE227 4.3 9.5 1.0 ND1 B:HIS42 4.3 11.4 1.0 CE1 B:HIS231 4.3 7.8 1.0 CZ B:PHE218 4.4 17.8 1.0 CG B:HIS69 4.4 5.2 1.0 O B:ALA59 4.7 8.6 1.0 CB B:ALA59 4.8 11.8 1.0 CB B:HIS60 4.9 15.6 1.0 CE1 B:PHE65 5.0 8.5 1.0

M.Goldfeder, M.Kanteev, N.Adir, A.Fishman. Influencing the Monophenolase/Diphenolase Activity Ratio in Tyrosinase. Biochim.Biophys.Acta V.1834 629 2013.
ISSN: ISSN 0006-3002
PubMed: 23305929
DOI: 10.1016/J.BBAPAP.2012.12.021