Copper in PDB 3lzo: Crystal Structure Analysis of the Copper-Reconstituted P19 Protein From Campylobacter Jejuni at 1.65 A at pH 10.0

The structure of Crystal Structure Analysis of the Copper-Reconstituted P19 Protein From Campylobacter Jejuni at 1.65 A at pH 10.0, PDB code: 3lzo was solved by T.I.Doukov, A.C.K.Chan, M.Scofield, A.B.Ramin, S.A.L.Tom-Yew, M.E.P.Murphy, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.34 / 1.65
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	55.831, 72.581, 78.817, 90.00, 90.00, 90.00
R / Rfree (%)	13.9 / 18.7

The binding sites of Copper atom in the Crystal Structure Analysis of the Copper-Reconstituted P19 Protein From Campylobacter Jejuni at 1.65 A at pH 10.0 (pdb code 3lzo). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 2 binding sites of Copper where determined in the Crystal Structure Analysis of the Copper-Reconstituted P19 Protein From Campylobacter Jejuni at 1.65 A at pH 10.0, PDB code: 3lzo:
Jump to Copper binding site number: 1; 2;

Copper binding site 1 out of 2 in the Crystal Structure Analysis of the Copper-Reconstituted P19 Protein From Campylobacter Jejuni at 1.65 A at pH 10.0


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of Crystal Structure Analysis of the Copper-Reconstituted P19 Protein From Campylobacter Jejuni at 1.65 A at pH 10.0 within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu200 b:26.3 occ:1.00 NE2 A:HIS42 2.0 20.8 1.0 NE2 A:HIS95 2.0 18.7 1.0 NE2 B:HIS132 2.1 24.2 1.0 OE1 A:GLU44 2.3 24.5 0.7 SD A:MET88 2.7 22.9 1.0 OE2 A:GLU44 2.8 34.5 0.7 CD A:GLU44 2.9 26.4 0.7 CD2 A:HIS42 2.9 18.4 1.0 CE1 B:HIS132 2.9 22.4 1.0 CE1 A:HIS42 3.0 25.4 1.0 CD2 A:HIS95 3.0 20.2 1.0 CE1 A:HIS95 3.0 27.3 1.0 OE2 A:GLU44 3.1 13.1 0.3 CD2 B:HIS132 3.2 26.5 1.0 CD A:GLU44 3.7 15.5 0.3 CG A:GLU44 3.7 15.8 0.3 CG A:MET88 3.8 22.2 1.0 ND1 A:HIS42 4.1 21.8 1.0 CG A:HIS42 4.1 21.1 1.0 ND1 A:HIS95 4.1 24.2 1.0 CG A:HIS95 4.1 21.7 1.0 ND1 B:HIS132 4.1 22.4 1.0 CE A:MET88 4.2 21.7 1.0 CA A:GLY97 4.3 17.9 1.0 CG B:HIS132 4.3 23.5 1.0 CG A:GLU44 4.4 22.7 0.7 OE1 A:GLU44 4.7 18.5 0.3 N A:GLY97 4.8 18.2 1.0 CB A:GLU44 4.9 18.9 0.3 SD A:MET27 5.0 28.0 1.0

Copper binding site 2 out of 2 in the Crystal Structure Analysis of the Copper-Reconstituted P19 Protein From Campylobacter Jejuni at 1.65 A at pH 10.0


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of Crystal Structure Analysis of the Copper-Reconstituted P19 Protein From Campylobacter Jejuni at 1.65 A at pH 10.0 within 5.0Å range: probe atom residue distance (Å) B Occ B:Cu200 b:25.6 occ:1.00 NE2 B:HIS42 2.0 24.1 1.0 NE2 B:HIS95 2.0 20.2 1.0 NE2 A:HIS132 2.1 19.0 1.0 OE1 B:GLU44 2.2 35.2 1.0 SD B:MET88 2.8 23.5 1.0 CD B:GLU44 2.9 31.6 1.0 OE2 B:GLU44 2.9 25.4 1.0 CD2 B:HIS42 2.9 26.6 1.0 CD2 B:HIS95 3.0 21.7 1.0 CE1 A:HIS132 3.0 24.5 1.0 CE1 B:HIS95 3.0 25.8 1.0 CE1 B:HIS42 3.0 22.9 1.0 CD2 A:HIS132 3.1 20.6 1.0 CG B:MET88 3.8 19.5 1.0 CG B:HIS42 4.1 21.4 1.0 ND1 B:HIS95 4.1 22.4 1.0 ND1 B:HIS42 4.1 24.3 1.0 CE B:MET88 4.1 22.4 1.0 CG B:HIS95 4.1 22.9 1.0 ND1 A:HIS132 4.2 20.5 1.0 CG A:HIS132 4.3 22.5 1.0 CG B:GLU44 4.3 30.5 1.0 CA B:GLY97 4.5 21.2 1.0 O B:HOH1135 4.9 34.9 1.0 N B:GLY97 5.0 19.0 1.0

A.C.Chan, T.I.Doukov, M.Scofield, S.A.Tom-Yew, A.B.Ramin, J.K.Mackichan, E.C.Gaynor, M.E.Murphy. Structure and Function of P19, A High-Affinity Iron Transporter of the Human Pathogen Campylobacter Jejuni. J.Mol.Biol. V. 401 590 2010.
ISSN: ISSN 0022-2836
PubMed: 20600116
DOI: 10.1016/J.JMB.2010.06.038