Copper in PDB 1etj: Azurin Mutant with Met 121 Replaced By Glu

The structure of Azurin Mutant with Met 121 Replaced By Glu, PDB code: 1etj was solved by B.G.Karlsson, L.-C.Tsai, H.Nar, J.Sanders-Loehr, N.Bonander, V.Langer, L.Sjolin, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	N/A / 2.30
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	50.580, 60.940, 81.600, 90.00, 90.10, 90.00
R / Rfree (%)	18.4 / n/a

The binding sites of Copper atom in the Azurin Mutant with Met 121 Replaced By Glu (pdb code 1etj). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 4 binding sites of Copper where determined in the Azurin Mutant with Met 121 Replaced By Glu, PDB code: 1etj:
Jump to Copper binding site number: 1; 2; 3; 4;

Copper binding site 1 out of 4 in the Azurin Mutant with Met 121 Replaced By Glu


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of Azurin Mutant with Met 121 Replaced By Glu within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu129 b:18.3 occ:1.00 ND1 A:HIS117 2.0 14.8 1.0 SG A:CYS112 2.0 11.6 1.0 OE1 A:GLU121 2.2 20.9 1.0 ND1 A:HIS46 2.2 14.7 1.0 CG A:HIS117 3.0 12.7 1.0 CE1 A:HIS117 3.1 14.5 1.0 CE1 A:HIS46 3.1 17.3 1.0 CG A:HIS46 3.2 11.7 1.0 CB A:HIS117 3.2 16.9 1.0 CB A:CYS112 3.2 10.1 1.0 O A:GLY45 3.3 14.7 1.0 CD A:GLU121 3.4 20.0 1.0 CA A:HIS46 3.6 5.8 1.0 CB A:HIS46 3.6 9.6 1.0 OE2 A:GLU121 4.1 19.3 1.0 NE2 A:HIS117 4.2 10.3 1.0 C A:GLY45 4.2 9.7 1.0 CD2 A:HIS117 4.2 13.7 1.0 CB A:PHE114 4.2 2.0 1.0 NE2 A:HIS46 4.3 13.7 1.0 N A:HIS46 4.3 9.4 1.0 CD2 A:HIS46 4.4 11.9 1.0 CG A:GLU121 4.4 12.2 1.0 CA A:CYS112 4.5 5.7 1.0 CA A:HIS117 4.6 14.8 1.0 N A:ASN47 4.6 5.7 1.0 C A:HIS46 4.7 4.4 1.0 CB A:GLU121 4.7 13.3 1.0 C A:CYS112 4.9 6.0 1.0 CG A:PHE114 4.9 3.7 1.0

Copper binding site 2 out of 4 in the Azurin Mutant with Met 121 Replaced By Glu


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of Azurin Mutant with Met 121 Replaced By Glu within 5.0Å range: probe atom residue distance (Å) B Occ B:Cu129 b:19.6 occ:1.00 OE1 B:GLU121 1.9 19.3 1.0 ND1 B:HIS117 1.9 16.4 1.0 SG B:CYS112 2.1 12.2 1.0 ND1 B:HIS46 2.2 14.6 1.0 O B:GLY45 2.9 14.7 1.0 CG B:HIS117 2.9 16.1 1.0 CE1 B:HIS117 2.9 15.4 1.0 CE1 B:HIS46 3.1 13.0 1.0 CD B:GLU121 3.1 22.1 1.0 CG B:HIS46 3.2 13.2 1.0 CB B:HIS117 3.3 11.4 1.0 CB B:CYS112 3.3 9.3 1.0 CA B:HIS46 3.5 6.8 1.0 CB B:HIS46 3.6 8.8 1.0 OE2 B:GLU121 3.7 18.5 1.0 C B:GLY45 3.9 13.8 1.0 NE2 B:HIS117 4.1 15.9 1.0 CD2 B:HIS117 4.1 16.6 1.0 N B:HIS46 4.2 12.7 1.0 NE2 B:HIS46 4.2 10.6 1.0 CG B:GLU121 4.2 18.0 1.0 CB B:PHE114 4.3 3.5 1.0 CD2 B:HIS46 4.4 5.1 1.0 N B:ASN47 4.6 5.7 1.0 CA B:CYS112 4.6 6.8 1.0 CA B:HIS117 4.7 14.2 1.0 C B:HIS46 4.7 4.5 1.0 CB B:GLU121 4.8 18.1 1.0

Copper binding site 3 out of 4 in the Azurin Mutant with Met 121 Replaced By Glu


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 3 of Azurin Mutant with Met 121 Replaced By Glu within 5.0Å range: probe atom residue distance (Å) B Occ C:Cu129 b:18.6 occ:1.00 OE1 C:GLU121 1.9 19.6 1.0 ND1 C:HIS117 1.9 18.5 1.0 SG C:CYS112 1.9 15.7 1.0 ND1 C:HIS46 2.2 16.9 1.0 CE1 C:HIS117 2.9 17.9 1.0 CG C:HIS117 2.9 18.0 1.0 CD C:GLU121 3.1 21.3 1.0 CE1 C:HIS46 3.1 14.2 1.0 O C:GLY45 3.1 14.3 1.0 CB C:CYS112 3.1 13.7 1.0 CG C:HIS46 3.2 14.9 1.0 CB C:HIS117 3.2 13.3 1.0 CA C:HIS46 3.6 10.0 1.0 CB C:HIS46 3.6 14.0 1.0 OE2 C:GLU121 3.8 24.1 1.0 NE2 C:HIS117 4.0 15.5 1.0 CD2 C:HIS117 4.1 17.8 1.0 C C:GLY45 4.1 13.3 1.0 CB C:PHE114 4.1 13.3 1.0 CG C:GLU121 4.2 21.8 1.0 NE2 C:HIS46 4.2 15.7 1.0 N C:HIS46 4.3 12.7 1.0 CD2 C:HIS46 4.4 14.9 1.0 CA C:CYS112 4.5 12.5 1.0 N C:ASN47 4.6 9.5 1.0 CA C:HIS117 4.7 17.6 1.0 C C:HIS46 4.7 9.8 1.0 CB C:GLU121 4.7 23.8 1.0 CG C:PHE114 4.9 15.6 1.0 C C:CYS112 5.0 10.0 1.0

Copper binding site 4 out of 4 in the Azurin Mutant with Met 121 Replaced By Glu


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 4 of Azurin Mutant with Met 121 Replaced By Glu within 5.0Å range: probe atom residue distance (Å) B Occ D:Cu129 b:22.4 occ:1.00 ND1 D:HIS46 1.9 21.4 1.0 OE1 D:GLU121 2.0 22.1 1.0 ND1 D:HIS117 2.2 22.6 1.0 SG D:CYS112 2.2 12.1 1.0 CE1 D:HIS46 2.9 24.6 1.0 CG D:HIS46 2.9 19.3 1.0 CB D:CYS112 3.0 12.2 1.0 CD D:GLU121 3.2 23.2 1.0 CG D:HIS117 3.2 20.2 1.0 CE1 D:HIS117 3.2 25.0 1.0 CB D:HIS46 3.4 16.4 1.0 O D:GLY45 3.4 17.4 1.0 CB D:HIS117 3.5 18.6 1.0 CA D:HIS46 3.6 13.3 1.0 OE2 D:GLU121 3.8 25.1 1.0 NE2 D:HIS46 4.0 24.4 1.0 CB D:PHE114 4.0 16.4 1.0 CD2 D:HIS46 4.1 20.4 1.0 C D:GLY45 4.2 16.2 1.0 CG D:GLU121 4.3 18.4 1.0 NE2 D:HIS117 4.3 22.7 1.0 N D:HIS46 4.3 14.7 1.0 CD2 D:HIS117 4.4 24.5 1.0 CA D:CYS112 4.5 10.7 1.0 N D:ASN47 4.6 14.9 1.0 C D:HIS46 4.7 13.9 1.0 CB D:GLU121 4.7 21.2 1.0 CG D:PHE114 4.8 19.6 1.0 CA D:HIS117 4.9 15.6 1.0

B.G.Karlsson, L.C.Tsai, H.Nar, J.Sanders-Loehr, N.Bonander, V.Langer, L.Sjolin. X-Ray Structure Determination and Characterization of the Pseudomonas Aeruginosa Azurin Mutant MET121GLU. Biochemistry V. 36 4089 1997.
ISSN: ISSN 0006-2960
PubMed: 9100002
DOI: 10.1021/BI962416O