Copper in PDB 9ix9: Mutant H286T Crystal Structure of Two-Domain Bacterial Laccase From the Actinobacterium Streptomyces Carpinensis Vkm Ac-1300

The structure of Mutant H286T Crystal Structure of Two-Domain Bacterial Laccase From the Actinobacterium Streptomyces Carpinensis Vkm Ac-1300, PDB code: 9ix9 was solved by A.G.Gabdulkhakov, T.V.Tishchenko, L.Trubitsina, I.Trubitsin, A.Leontievsky, A.Lisov, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	25.81 / 2.05
Space group	I 2 3
Cell size a, b, c (Å), α, β, γ (°)	196.546, 196.546, 196.546, 90, 90, 90
R / Rfree (%)	20.9 / 25.8

Pages:

>>> Page 1 <<< Page 2, Binding sites: 11 - 12;

Binding sites:

The binding sites of Copper atom in the Mutant H286T Crystal Structure of Two-Domain Bacterial Laccase From the Actinobacterium Streptomyces Carpinensis Vkm Ac-1300 (pdb code 9ix9). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 12 binding sites of Copper where determined in the Mutant H286T Crystal Structure of Two-Domain Bacterial Laccase From the Actinobacterium Streptomyces Carpinensis Vkm Ac-1300, PDB code: 9ix9:
Jump to Copper binding site number: 1; 2; 3; 4; 5; 6; 7; 8; 9; 10;

Copper binding site 1 out of 12 in the Mutant H286T Crystal Structure of Two-Domain Bacterial Laccase From the Actinobacterium Streptomyces Carpinensis Vkm Ac-1300


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of Mutant H286T Crystal Structure of Two-Domain Bacterial Laccase From the Actinobacterium Streptomyces Carpinensis Vkm Ac-1300 within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu401 b:22.8 occ:1.00 ND1 A:HIS296 2.0 21.1 1.0 ND1 A:HIS234 2.1 13.9 1.0 SG A:CYS291 2.2 17.2 1.0 CG A:HIS296 3.0 22.4 1.0 CG A:HIS234 3.0 14.6 1.0 CE1 A:HIS296 3.0 17.3 1.0 CE1 A:HIS234 3.1 17.0 1.0 CB A:CYS291 3.2 16.6 1.0 CB A:HIS296 3.3 17.3 1.0 CB A:HIS234 3.3 15.8 1.0 SD A:MET301 3.4 18.7 1.0 CA A:HIS234 3.6 20.5 1.0 O A:PHE233 3.9 14.1 1.0 CG2 A:VAL293 4.0 16.0 1.0 CB A:VAL293 4.0 15.7 1.0 CE A:MET301 4.0 13.1 1.0 CD2 A:HIS296 4.1 20.4 1.0 NE2 A:HIS296 4.1 18.8 1.0 CD2 A:HIS234 4.2 17.2 1.0 NE2 A:HIS234 4.2 17.2 1.0 N A:HIS234 4.5 20.1 1.0 C A:PHE233 4.6 15.6 1.0 CA A:CYS291 4.7 15.5 1.0 N A:THR235 4.7 16.7 1.0 C A:HIS234 4.8 20.9 1.0 CA A:HIS296 4.8 18.6 1.0 CG1 A:VAL293 4.9 19.9 1.0 CE A:MET201 4.9 28.3 1.0 CG A:MET301 5.0 21.9 1.0 N A:VAL293 5.0 16.8 1.0

Copper binding site 2 out of 12 in the Mutant H286T Crystal Structure of Two-Domain Bacterial Laccase From the Actinobacterium Streptomyces Carpinensis Vkm Ac-1300


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of Mutant H286T Crystal Structure of Two-Domain Bacterial Laccase From the Actinobacterium Streptomyces Carpinensis Vkm Ac-1300 within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu402 b:27.4 occ:1.00 NE2 A:HIS239 2.1 17.6 1.0 NE2 C:HIS161 2.2 19.2 1.0 O2 A:OXY403 2.2 23.2 1.0 NE2 A:HIS290 2.4 23.4 1.0 O1 A:OXY403 2.6 20.8 1.0 CE1 A:HIS239 2.8 20.1 1.0 CD2 C:HIS161 3.2 16.9 1.0 CE1 C:HIS161 3.2 17.7 1.0 CD2 A:HIS290 3.2 26.4 1.0 CD2 A:HIS239 3.3 21.8 1.0 CE1 A:HIS290 3.3 21.5 1.0 O A:HOH564 3.7 27.1 1.0 ND1 A:HIS239 4.0 21.5 1.0 CG A:HIS239 4.3 21.6 1.0 ND1 C:HIS161 4.3 16.2 1.0 CD2 A:HIS237 4.3 16.6 1.0 CG C:HIS161 4.3 19.9 1.0 CG A:HIS290 4.3 22.7 1.0 ND1 A:HIS290 4.3 19.1 1.0 NE2 C:HIS105 4.6 24.7 1.0 ND1 C:HIS167 4.7 26.4 1.0 NE2 A:HIS237 4.7 18.4 1.0 CE1 C:HIS105 4.8 25.5 1.0 CB A:MET288 4.9 25.6 1.0 CD2 C:HIS105 5.0 18.9 1.0

Copper binding site 3 out of 12 in the Mutant H286T Crystal Structure of Two-Domain Bacterial Laccase From the Actinobacterium Streptomyces Carpinensis Vkm Ac-1300


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 3 of Mutant H286T Crystal Structure of Two-Domain Bacterial Laccase From the Actinobacterium Streptomyces Carpinensis Vkm Ac-1300 within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu405 b:29.1 occ:1.00 NE2 A:HIS159 2.0 18.9 1.0 NE2 B:HIS292 2.1 16.2 1.0 NE2 A:HIS107 2.2 19.7 1.0 CE1 A:HIS159 2.9 19.3 1.0 O2 B:OXY403 2.9 25.1 1.0 O1 B:OXY403 3.0 19.1 1.0 CD2 B:HIS292 3.0 13.8 1.0 CD2 A:HIS159 3.1 15.4 1.0 CE1 B:HIS292 3.1 14.5 1.0 CD2 A:HIS107 3.1 16.9 1.0 CE1 A:HIS107 3.2 17.7 1.0 CE1 A:HIS105 3.3 20.9 1.0 ND1 A:HIS105 3.3 21.5 1.0 ND1 A:HIS159 4.1 19.6 1.0 CG A:HIS159 4.2 17.1 1.0 CG B:HIS292 4.2 17.6 1.0 CB A:ALA269 4.2 13.1 1.0 ND1 B:HIS292 4.2 19.0 1.0 CE1 A:HIS157 4.3 14.7 1.0 ND1 A:HIS107 4.3 16.9 1.0 CG A:HIS107 4.3 17.7 1.0 NE2 B:HIS237 4.5 23.2 1.0 NE2 A:HIS105 4.6 30.3 1.0 CD2 B:HIS237 4.6 18.9 1.0 O B:HOH545 4.7 23.5 1.0 CG A:HIS105 4.7 19.4 1.0 NE2 A:HIS157 4.9 17.0 1.0 CE1 A:HIS229 5.0 18.8 1.0

Copper binding site 4 out of 12 in the Mutant H286T Crystal Structure of Two-Domain Bacterial Laccase From the Actinobacterium Streptomyces Carpinensis Vkm Ac-1300


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 4 of Mutant H286T Crystal Structure of Two-Domain Bacterial Laccase From the Actinobacterium Streptomyces Carpinensis Vkm Ac-1300 within 5.0Å range: probe atom residue distance (Å) B Occ B:Cu401 b:23.4 occ:1.00 ND1 B:HIS296 2.0 18.6 1.0 ND1 B:HIS234 2.1 18.8 1.0 SG B:CYS291 2.2 17.0 1.0 CE1 B:HIS296 3.0 22.9 1.0 CG B:HIS234 3.0 18.0 1.0 CG B:HIS296 3.0 21.3 1.0 CE1 B:HIS234 3.1 21.9 1.0 CB B:CYS291 3.2 16.4 1.0 CB B:HIS234 3.3 15.9 1.0 CB B:HIS296 3.3 19.1 1.0 SD B:MET301 3.5 22.9 1.0 CA B:HIS234 3.6 21.4 1.0 CE B:MET301 3.7 16.1 1.0 CG2 B:VAL293 3.8 15.9 1.0 CB B:VAL293 4.0 15.6 1.0 O B:PHE233 4.0 18.8 1.0 NE2 B:HIS296 4.1 22.2 1.0 CD2 B:HIS296 4.1 21.0 1.0 CD2 B:HIS234 4.2 16.6 1.0 NE2 B:HIS234 4.2 17.2 1.0 N B:HIS234 4.5 18.5 1.0 C B:PHE233 4.6 17.8 1.0 N B:THR235 4.6 16.5 1.0 CA B:CYS291 4.6 21.1 1.0 C B:HIS234 4.7 20.0 1.0 CG1 B:VAL293 4.8 14.8 1.0 CA B:HIS296 4.9 18.6 1.0 N B:VAL293 4.9 12.9 1.0

Copper binding site 5 out of 12 in the Mutant H286T Crystal Structure of Two-Domain Bacterial Laccase From the Actinobacterium Streptomyces Carpinensis Vkm Ac-1300


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 5 of Mutant H286T Crystal Structure of Two-Domain Bacterial Laccase From the Actinobacterium Streptomyces Carpinensis Vkm Ac-1300 within 5.0Å range: probe atom residue distance (Å) B Occ B:Cu402 b:27.4 occ:1.00 NE2 B:HIS239 2.1 23.0 1.0 O1 B:OXY403 2.2 19.1 1.0 NE2 A:HIS161 2.2 24.3 1.0 NE2 B:HIS290 2.3 29.3 1.0 O2 B:OXY403 2.3 25.1 1.0 CE1 B:HIS239 2.8 27.6 1.0 CD2 A:HIS161 3.2 21.7 1.0 CE1 B:HIS290 3.2 27.0 1.0 CE1 A:HIS161 3.2 19.8 1.0 CD2 B:HIS290 3.2 28.2 1.0 CD2 B:HIS239 3.3 21.3 1.0 O B:HOH545 3.9 23.5 1.0 ND1 B:HIS239 4.0 18.3 1.0 CG B:HIS239 4.3 21.8 1.0 ND1 B:HIS290 4.3 24.9 1.0 CD2 B:HIS237 4.3 18.9 1.0 CG A:HIS161 4.3 23.5 1.0 ND1 A:HIS161 4.3 18.1 1.0 CG B:HIS290 4.4 25.4 1.0 CE1 A:HIS105 4.5 20.9 1.0 NE2 A:HIS105 4.6 30.3 1.0 NE2 B:HIS237 4.8 23.2 1.0 ND1 A:HIS167 4.8 26.2 1.0 CB B:MET288 4.9 29.1 1.0 ND1 A:HIS105 4.9 21.5 1.0

Copper binding site 6 out of 12 in the Mutant H286T Crystal Structure of Two-Domain Bacterial Laccase From the Actinobacterium Streptomyces Carpinensis Vkm Ac-1300


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 6 of Mutant H286T Crystal Structure of Two-Domain Bacterial Laccase From the Actinobacterium Streptomyces Carpinensis Vkm Ac-1300 within 5.0Å range: probe atom residue distance (Å) B Occ B:Cu406 b:27.4 occ:1.00 NE2 C:HIS292 2.1 20.4 1.0 CE1 B:HIS107 2.1 20.0 1.0 NE2 B:HIS159 2.2 17.5 1.0 O2 C:OXY404 2.6 18.0 1.0 NE2 B:HIS107 3.0 21.5 1.0 CD2 C:HIS292 3.0 16.2 1.0 CE1 C:HIS292 3.0 20.1 1.0 O1 C:OXY404 3.1 26.3 1.0 CE1 B:HIS159 3.1 19.8 1.0 CD2 B:HIS159 3.2 18.9 1.0 ND1 B:HIS107 3.2 24.4 1.0 CD2 B:HIS105 3.3 20.3 1.0 NE2 B:HIS105 3.3 23.4 1.0 ND1 C:HIS292 4.1 15.4 1.0 CG C:HIS292 4.2 17.8 1.0 CB B:ALA269 4.2 16.5 1.0 CD2 B:HIS107 4.2 15.6 1.0 CE1 B:HIS157 4.2 19.3 1.0 ND1 B:HIS159 4.2 19.6 1.0 CG B:HIS159 4.3 19.4 1.0 CG B:HIS107 4.3 17.8 1.0 NE2 C:HIS237 4.4 18.4 1.0 CD2 C:HIS237 4.6 19.3 1.0 CG B:HIS105 4.6 22.6 1.0 CE1 B:HIS105 4.6 24.7 1.0 NE2 B:HIS157 5.0 16.3 1.0

Copper binding site 7 out of 12 in the Mutant H286T Crystal Structure of Two-Domain Bacterial Laccase From the Actinobacterium Streptomyces Carpinensis Vkm Ac-1300


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 7 of Mutant H286T Crystal Structure of Two-Domain Bacterial Laccase From the Actinobacterium Streptomyces Carpinensis Vkm Ac-1300 within 5.0Å range: probe atom residue distance (Å) B Occ C:Cu401 b:28.2 occ:1.00 NE2 C:HIS159 2.0 17.8 1.0 NE2 A:HIS292 2.1 18.5 1.0 CE1 C:HIS107 2.1 13.1 1.0 O1 A:OXY403 2.7 20.8 1.0 CE1 C:HIS159 2.9 18.1 1.0 NE2 C:HIS107 3.0 15.2 1.0 O2 A:OXY403 3.0 23.2 1.0 CD2 A:HIS292 3.1 17.2 1.0 CD2 C:HIS159 3.1 17.8 1.0 CE1 A:HIS292 3.1 20.2 1.0 ND1 C:HIS107 3.2 17.6 1.0 CD2 C:HIS105 3.4 18.9 1.0 NE2 C:HIS105 3.6 24.7 1.0 ND1 C:HIS159 4.1 16.7 1.0 CG C:HIS159 4.2 19.3 1.0 ND1 A:HIS292 4.2 18.7 1.0 CG A:HIS292 4.2 20.2 1.0 CD2 C:HIS107 4.2 14.7 1.0 CB C:ALA269 4.3 16.6 1.0 CG C:HIS107 4.3 13.1 1.0 CE1 C:HIS157 4.3 15.5 1.0 NE2 A:HIS237 4.5 18.4 1.0 CD2 A:HIS237 4.6 16.6 1.0 CG C:HIS105 4.7 19.2 1.0 O A:HOH564 4.7 27.1 1.0 CE1 C:HIS105 4.9 25.5 1.0

Copper binding site 8 out of 12 in the Mutant H286T Crystal Structure of Two-Domain Bacterial Laccase From the Actinobacterium Streptomyces Carpinensis Vkm Ac-1300


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 8 of Mutant H286T Crystal Structure of Two-Domain Bacterial Laccase From the Actinobacterium Streptomyces Carpinensis Vkm Ac-1300 within 5.0Å range: probe atom residue distance (Å) B Occ C:Cu402 b:22.0 occ:1.00 ND1 C:HIS296 2.0 17.7 1.0 ND1 C:HIS234 2.1 14.5 1.0 SG C:CYS291 2.2 15.7 1.0 CG C:HIS296 2.9 17.7 1.0 CE1 C:HIS296 3.0 22.2 1.0 CG C:HIS234 3.1 17.7 1.0 CE1 C:HIS234 3.1 20.2 1.0 CB C:CYS291 3.2 15.3 1.0 CB C:HIS296 3.3 19.0 1.0 CB C:HIS234 3.3 15.0 1.0 SD C:MET301 3.4 21.7 1.0 CA C:HIS234 3.7 18.2 1.0 CG2 C:VAL293 3.9 14.3 1.0 CB C:VAL293 3.9 14.3 1.0 O C:PHE233 4.0 18.7 1.0 CE C:MET301 4.0 19.3 1.0 NE2 C:HIS296 4.1 19.0 1.0 CD2 C:HIS296 4.1 19.6 1.0 CD2 C:HIS234 4.2 16.3 1.0 NE2 C:HIS234 4.2 18.7 1.0 N C:HIS234 4.6 17.6 1.0 CA C:CYS291 4.6 19.1 1.0 N C:THR235 4.7 15.8 1.0 C C:PHE233 4.7 17.8 1.0 C C:HIS234 4.8 21.6 1.0 CG1 C:VAL293 4.8 16.5 1.0 CA C:HIS296 4.8 18.6 1.0 N C:VAL293 4.9 17.2 1.0 CG C:MET301 5.0 21.6 1.0 CA C:VAL293 5.0 16.2 1.0

Copper binding site 9 out of 12 in the Mutant H286T Crystal Structure of Two-Domain Bacterial Laccase From the Actinobacterium Streptomyces Carpinensis Vkm Ac-1300


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 9 of Mutant H286T Crystal Structure of Two-Domain Bacterial Laccase From the Actinobacterium Streptomyces Carpinensis Vkm Ac-1300 within 5.0Å range: probe atom residue distance (Å) B Occ C:Cu403 b:29.4 occ:1.00 NE2 C:HIS239 2.1 25.8 1.0 O1 C:OXY404 2.2 26.3 1.0 NE2 B:HIS161 2.3 18.9 1.0 NE2 C:HIS290 2.3 29.3 1.0 O2 C:OXY404 2.6 18.0 1.0 CE1 C:HIS239 2.8 24.6 1.0 CD2 B:HIS161 3.2 23.3 1.0 CE1 C:HIS290 3.2 26.4 1.0 CD2 C:HIS290 3.2 28.6 1.0 CD2 C:HIS239 3.3 24.1 1.0 CE1 B:HIS161 3.3 21.0 1.0 ND1 C:HIS239 4.1 19.8 1.0 ND1 C:HIS290 4.3 24.3 1.0 CG C:HIS239 4.3 22.4 1.0 CG C:HIS290 4.3 25.4 1.0 CD2 C:HIS237 4.3 19.3 1.0 CG B:HIS161 4.4 19.3 1.0 ND1 B:HIS161 4.4 18.7 1.0 NE2 B:HIS105 4.5 23.4 1.0 CE1 B:HIS105 4.7 24.7 1.0 CD2 B:HIS167 4.7 27.6 1.0 NE2 C:HIS237 4.8 18.4 1.0 NE2 C:HIS292 5.0 20.4 1.0

Copper binding site 10 out of 12 in the Mutant H286T Crystal Structure of Two-Domain Bacterial Laccase From the Actinobacterium Streptomyces Carpinensis Vkm Ac-1300


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 10 of Mutant H286T Crystal Structure of Two-Domain Bacterial Laccase From the Actinobacterium Streptomyces Carpinensis Vkm Ac-1300 within 5.0Å range: probe atom residue distance (Å) B Occ D:Cu401 b:19.6 occ:1.00 ND1 D:HIS296 1.9 13.5 1.0 ND1 D:HIS234 2.2 12.6 1.0 SG D:CYS291 2.2 14.3 1.0 CE1 D:HIS296 2.9 14.4 1.0 CG D:HIS296 2.9 15.6 1.0 CG D:HIS234 3.1 15.0 1.0 CE1 D:HIS234 3.2 12.1 1.0 CB D:CYS291 3.2 12.1 1.0 CB D:HIS296 3.3 12.2 1.0 SD D:MET301 3.3 18.5 1.0 CB D:HIS234 3.3 11.3 1.0 CA D:HIS234 3.7 15.1 1.0 CE D:MET301 3.8 15.3 1.0 CG2 D:VAL293 4.0 13.2 1.0 O D:PHE233 4.0 12.1 1.0 NE2 D:HIS296 4.0 15.6 1.0 CB D:VAL293 4.0 12.1 1.0 CD2 D:HIS296 4.0 16.0 1.0 CD2 D:HIS234 4.3 15.8 1.0 NE2 D:HIS234 4.3 13.1 1.0 CA D:CYS291 4.6 14.2 1.0 N D:HIS234 4.6 13.0 1.0 C D:PHE233 4.7 13.8 1.0 N D:THR235 4.7 10.6 1.0 CA D:HIS296 4.8 15.9 1.0 C D:HIS234 4.8 14.8 1.0 CG1 D:VAL293 4.9 13.1 1.0 CG D:MET301 4.9 21.0 1.0 N D:VAL293 5.0 11.8 1.0

A.G.Gabdulkhakov, T.V.Tishchenko, L.Trubitsina, I.Trubitsin, A.Leontievsky, A.Lisov. Mutant H286T Crystal Structure of Two-Domain Bacterial Laccase From the Actinobacterium Streptomyces Carpinensis Vkm Ac-1300 To Be Published.