Copper in PDB 9e6z: Streptavidin-E101Q-S112F-K121A Bound to Cu(II)-Biotin-Ethyl- Dipicolylamine Cofactor

The structure of Streptavidin-E101Q-S112F-K121A Bound to Cu(II)-Biotin-Ethyl- Dipicolylamine Cofactor, PDB code: 9e6z was solved by K.S.Uyeda, A.H.Follmer, A.S.Borovik, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	41.11 / 1.70
Space group	I 41 2 2
Cell size a, b, c (Å), α, β, γ (°)	57.536, 57.536, 176.282, 90, 90, 90
R / Rfree (%)	20.1 / 21.9

The binding sites of Copper atom in the Streptavidin-E101Q-S112F-K121A Bound to Cu(II)-Biotin-Ethyl- Dipicolylamine Cofactor (pdb code 9e6z). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total only one binding site of Copper was determined in the Streptavidin-E101Q-S112F-K121A Bound to Cu(II)-Biotin-Ethyl- Dipicolylamine Cofactor, PDB code: 9e6z:

Copper binding site 1 out of 1 in the Streptavidin-E101Q-S112F-K121A Bound to Cu(II)-Biotin-Ethyl- Dipicolylamine Cofactor


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of Streptavidin-E101Q-S112F-K121A Bound to Cu(II)-Biotin-Ethyl- Dipicolylamine Cofactor within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu203 b:24.3 occ:0.78 N4 A:QG7201 1.8 26.1 1.0 N5 A:QG7201 1.9 28.0 1.0 N3 A:QG7201 2.1 20.4 1.0 O A:ACY202 2.3 30.8 0.8 OD1 A:ASN49 2.5 31.4 0.6 C12 A:QG7201 2.6 22.2 1.0 C16 A:QG7201 2.7 28.7 1.0 O A:HOH301 2.8 41.9 1.0 C11 A:QG7201 2.8 25.4 1.0 C18 A:QG7201 2.8 28.8 1.0 H23 A:QG7201 2.8 25.9 1.0 C22 A:QG7201 2.8 32.6 1.0 C A:ACY202 2.9 44.4 0.8 H28 A:QG7201 2.9 34.4 1.0 H30 A:QG7201 3.0 39.2 1.0 C17 A:QG7201 3.0 20.7 1.0 OXT A:ACY202 3.0 51.8 0.8 H5 A:QG7201 3.1 30.5 1.0 C23 A:QG7201 3.1 23.3 1.0 H3 A:QG7201 3.3 24.9 1.0 H26 A:QG7201 3.3 28.0 1.0 C24 A:QG7201 3.4 21.6 1.0 CG A:ASN49 3.7 27.8 0.6 H6 A:QG7201 3.8 30.5 1.0 H4 A:QG7201 3.9 24.9 1.0 H22 A:QG7201 4.0 25.9 1.0 C13 A:QG7201 4.0 32.8 1.0 C15 A:QG7201 4.0 38.4 1.0 H25 A:QG7201 4.1 28.0 1.0 C21 A:QG7201 4.1 31.9 1.0 C19 A:QG7201 4.1 27.1 1.0 ND2 A:ASN49 4.2 28.0 0.6 CH3 A:ACY202 4.3 45.5 0.8 C14 A:QG7201 4.5 30.9 1.0 H2 A:ACY202 4.6 54.6 0.8 H1 A:ACY202 4.6 54.6 0.8 N6 A:QG7201 4.7 15.3 1.0 C20 A:QG7201 4.7 31.5 1.0 CB A:ASN49 4.7 20.1 0.4 H29 A:QG7201 4.8 39.4 1.0 CB A:ASN49 4.8 20.0 0.6 H2 A:QG7201 4.9 46.0 1.0 ND2 A:ASN49 4.9 24.6 0.4 H33 A:QG7201 5.0 32.5 1.0 H31 A:QG7201 5.0 38.3 1.0 O1 A:QG7201 5.0 15.3 1.0 H3 A:ACY202 5.0 54.6 0.8

K.S.Uyeda, A.H.Follmer, A.S.Borovik. Selective Oxidation of Active Site Aromatic Residues in Engineered Cu Proteins Chem Sci 2024.
ISSN: ESSN 2041-6539
DOI: 10.1039/D4SC06667G