Copper in PDB 9csu: Streptavidin-E101Q-S112Y-K121A Bound to Cu(II)-Biotin-Ethyl- Dipicolylamine Cofactor

The structure of Streptavidin-E101Q-S112Y-K121A Bound to Cu(II)-Biotin-Ethyl- Dipicolylamine Cofactor, PDB code: 9csu was solved by K.S.Uyeda, A.H.Follmer, A.S.Borovik, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	54.72 / 1.60
Space group	I 41 2 2
Cell size a, b, c (Å), α, β, γ (°)	57.7, 57.7, 178.05, 90, 90, 90
R / Rfree (%)	17.5 / 20.7

The binding sites of Copper atom in the Streptavidin-E101Q-S112Y-K121A Bound to Cu(II)-Biotin-Ethyl- Dipicolylamine Cofactor (pdb code 9csu). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total only one binding site of Copper was determined in the Streptavidin-E101Q-S112Y-K121A Bound to Cu(II)-Biotin-Ethyl- Dipicolylamine Cofactor, PDB code: 9csu:

Copper binding site 1 out of 1 in the Streptavidin-E101Q-S112Y-K121A Bound to Cu(II)-Biotin-Ethyl- Dipicolylamine Cofactor


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of Streptavidin-E101Q-S112Y-K121A Bound to Cu(II)-Biotin-Ethyl- Dipicolylamine Cofactor within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu203 b:20.2 occ:0.65 N4 A:QG7202 1.9 29.5 1.0 N5 A:QG7202 2.0 36.3 1.0 N3 A:QG7202 2.1 27.8 1.0 OXT A:ACY201 2.3 56.5 0.9 OD1 A:ASN49 2.5 30.4 0.7 C12 A:QG7202 2.7 26.7 1.0 C18 A:QG7202 2.8 34.3 1.0 C16 A:QG7202 2.8 34.5 1.0 C11 A:QG7202 2.8 25.7 1.0 C17 A:QG7202 2.9 30.9 1.0 O A:HOH301 2.9 42.2 0.8 H28 A:QG7202 3.0 41.4 1.0 C22 A:QG7202 3.0 38.8 1.0 H23 A:QG7202 3.0 24.7 1.0 C A:ACY201 3.0 60.4 0.9 C23 A:QG7202 3.1 25.6 1.0 H30 A:QG7202 3.1 46.6 1.0 H5 A:QG7202 3.2 30.8 1.0 O A:ACY201 3.2 65.2 0.9 H3 A:QG7202 3.2 37.0 1.0 H26 A:QG7202 3.2 30.8 1.0 CG A:ASN49 3.5 27.7 0.7 C24 A:QG7202 3.5 20.6 1.0 H6 A:QG7202 3.8 30.8 1.0 H4 A:QG7202 3.8 37.0 1.0 ND2 A:ASN49 4.0 30.2 0.7 H25 A:QG7202 4.0 30.8 1.0 C13 A:QG7202 4.1 29.4 1.0 C15 A:QG7202 4.1 35.8 1.0 H22 A:QG7202 4.1 24.7 1.0 C19 A:QG7202 4.2 36.3 1.0 C21 A:QG7202 4.3 41.4 1.0 CH3 A:ACY201 4.5 60.0 0.9 C14 A:QG7202 4.6 31.2 1.0 H2 A:ACY201 4.7 72.0 0.9 N6 A:QG7202 4.7 19.2 1.0 CB A:ASN49 4.7 23.3 0.7 CB A:ASN49 4.8 21.9 0.3 C20 A:QG7202 4.8 40.4 1.0 H29 A:QG7202 4.9 35.2 1.0 H3 A:ACY201 4.9 72.0 0.9 H33 A:QG7202 5.0 43.5 1.0 H2 A:QG7202 5.0 42.9 1.0

K.S.Uyeda, A.H.Follmer, A.S.Borovik. Selective Oxidation of Active Site Aromatic Residues in Engineered Cu Proteins Chem Sci 2024.
ISSN: ESSN 2041-6539
DOI: 10.1039/D4SC06667G