Atomistry » Copper » PDB 8yk7-9fdl » 9cpm
Atomistry »
  Copper »
    PDB 8yk7-9fdl »
      9cpm »

Copper in PDB 9cpm: Thermus Thermophilus HB27 Laccase (Tth-Lac) Mutant with Partial Deletion of Beta-Hairpin Sequence

Enzymatic activity of Thermus Thermophilus HB27 Laccase (Tth-Lac) Mutant with Partial Deletion of Beta-Hairpin Sequence

All present enzymatic activity of Thermus Thermophilus HB27 Laccase (Tth-Lac) Mutant with Partial Deletion of Beta-Hairpin Sequence:
1.10.3.2;

Protein crystallography data

The structure of Thermus Thermophilus HB27 Laccase (Tth-Lac) Mutant with Partial Deletion of Beta-Hairpin Sequence, PDB code: 9cpm was solved by J.Lee, B.Miranda-Zaragoza, C.Rodriguez-Almazan, N.C.J.Strynadka, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 51.17 / 1.60
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 51.927, 66.032, 74.057, 90, 100.14, 90
R / Rfree (%) 15.3 / 18.2

Copper Binding Sites:

The binding sites of Copper atom in the Thermus Thermophilus HB27 Laccase (Tth-Lac) Mutant with Partial Deletion of Beta-Hairpin Sequence (pdb code 9cpm). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 5 binding sites of Copper where determined in the Thermus Thermophilus HB27 Laccase (Tth-Lac) Mutant with Partial Deletion of Beta-Hairpin Sequence, PDB code: 9cpm:
Jump to Copper binding site number: 1; 2; 3; 4; 5;

Copper binding site 1 out of 5 in 9cpm

Go back to Copper Binding Sites List in 9cpm
Copper binding site 1 out of 5 in the Thermus Thermophilus HB27 Laccase (Tth-Lac) Mutant with Partial Deletion of Beta-Hairpin Sequence


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of Thermus Thermophilus HB27 Laccase (Tth-Lac) Mutant with Partial Deletion of Beta-Hairpin Sequence within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cu501

b:10.5
occ:1.00
 ND1 A:HIS450 2.0 10.0 1.0
ND1 A:HIS393 2.1 9.6 1.0
SG A:CYS445 2.2 12.8 1.0
CE1 A:HIS393 3.0 10.4 1.0
CE1 A:HIS450 3.0 8.8 1.0
CG A:HIS450 3.0 9.8 1.0
CG A:HIS393 3.1 9.9 1.0
CB A:CYS445 3.3 13.9 1.0
CB A:HIS450 3.3 9.6 1.0
CB A:HIS393 3.5 9.6 1.0
SD A:MET455 3.6 19.7 1.0
CA A:HIS393 3.9 8.7 1.0
CD1 A:ILE447 4.0 9.1 1.0
CB A:ILE447 4.0 8.3 1.0
NE2 A:HIS393 4.1 9.8 1.0
CD2 A:HIS450 4.1 9.6 1.0
NE2 A:HIS450 4.1 9.5 1.0
CD2 A:HIS393 4.2 9.5 1.0
CG1 A:ILE447 4.3 9.1 1.0
O A:ASP392 4.4 9.2 1.0
CD A:PRO394 4.4 8.6 1.0
CE A:MET455 4.5 16.8 1.0
CA A:CYS445 4.6 10.6 1.0
O A:ILE447 4.7 8.5 1.0
N A:ILE447 4.7 7.9 1.0
CA A:HIS450 4.8 9.5 1.0
CG2 A:ILE447 4.9 9.6 1.0
C A:HIS393 4.9 9.2 1.0
N A:HIS393 4.9 8.8 1.0
CA A:ILE447 4.9 8.4 1.0
N A:PRO394 5.0 9.2 1.0

Copper binding site 2 out of 5 in 9cpm

Go back to Copper Binding Sites List in 9cpm
Copper binding site 2 out of 5 in the Thermus Thermophilus HB27 Laccase (Tth-Lac) Mutant with Partial Deletion of Beta-Hairpin Sequence


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of Thermus Thermophilus HB27 Laccase (Tth-Lac) Mutant with Partial Deletion of Beta-Hairpin Sequence within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cu502

b:15.4
occ:1.00
 NE2 A:HIS95 2.0 12.3 1.0
NE2 A:HIS396 2.0 10.8 1.0
O A:HOH763 2.8 21.3 1.0
CD2 A:HIS95 2.9 13.2 1.0
CD2 A:HIS396 3.0 10.8 1.0
CE1 A:HIS396 3.0 9.7 1.0
CE1 A:HIS95 3.0 13.3 1.0
CD2 A:HIS398 3.1 15.3 1.0
NE2 A:HIS398 3.3 15.4 1.0
O A:HOH938 3.4 36.4 1.0
ND1 A:HIS97 3.6 9.7 1.0
CU A:CU503 3.7 22.0 1.0
CG A:HIS97 3.7 10.7 1.0
CG A:HIS398 3.8 13.4 1.0
CA A:HIS97 3.8 10.4 1.0
CE1 A:HIS398 3.9 14.1 1.0
CB A:HIS97 4.0 10.6 1.0
CU A:CU504 4.0 13.9 1.0
CG A:HIS95 4.0 12.7 1.0
ND1 A:HIS396 4.1 9.7 1.0
ND1 A:HIS95 4.1 12.3 1.0
CG A:HIS396 4.1 9.3 1.0
CE1 A:HIS97 4.1 11.1 1.0
ND1 A:HIS398 4.2 13.4 1.0
CD2 A:HIS97 4.2 11.8 1.0
N A:GLY98 4.3 12.2 1.0
NE2 A:HIS97 4.4 11.2 1.0
CA A:HIS398 4.5 13.5 1.0
O A:HOH755 4.6 22.5 1.0
CB A:HIS398 4.6 13.2 1.0
C A:HIS97 4.7 10.1 1.0
O A:HOH864 4.7 26.4 1.0
N A:HIS97 4.8 10.8 1.0
NE2 A:HIS444 4.9 11.4 1.0
N A:HIS398 4.9 11.6 1.0

Copper binding site 3 out of 5 in 9cpm

Go back to Copper Binding Sites List in 9cpm
Copper binding site 3 out of 5 in the Thermus Thermophilus HB27 Laccase (Tth-Lac) Mutant with Partial Deletion of Beta-Hairpin Sequence


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 3 of Thermus Thermophilus HB27 Laccase (Tth-Lac) Mutant with Partial Deletion of Beta-Hairpin Sequence within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cu503

b:22.0
occ:1.00
 NE2 A:HIS398 2.0 15.4 1.0
NE2 A:HIS137 2.0 24.4 1.0
NE2 A:HIS444 2.0 11.4 1.0
O A:HOH938 2.2 36.4 1.0
CD2 A:HIS137 2.9 25.8 1.0
CE1 A:HIS398 2.9 14.1 1.0
CD2 A:HIS444 2.9 11.1 1.0
CD2 A:HIS398 3.0 15.3 1.0
CE1 A:HIS444 3.0 12.1 1.0
CE1 A:HIS137 3.1 26.9 1.0
O A:HOH652 3.7 30.3 1.0
CU A:CU502 3.7 15.4 1.0
CD2 A:HIS396 3.9 10.8 1.0
CD2 A:HIS95 4.0 13.2 1.0
ND1 A:HIS398 4.0 13.4 1.0
CG A:HIS398 4.1 13.4 1.0
CG A:HIS137 4.1 20.2 1.0
NE2 A:HIS95 4.1 12.3 1.0
ND1 A:HIS444 4.1 10.7 1.0
CG A:HIS444 4.1 10.0 1.0
ND1 A:HIS137 4.1 26.9 1.0
CG2 A:VAL442 4.3 13.5 1.0
NE2 A:HIS396 4.3 10.8 1.0
OE2 A:GLU451 4.5 26.4 1.0
CG A:HIS95 4.8 12.7 1.0
CE1 A:HIS95 4.8 13.3 1.0
OE1 A:GLU451 4.9 30.5 1.0
CD A:GLU451 4.9 22.8 1.0
CU A:CU504 5.0 13.9 1.0

Copper binding site 4 out of 5 in 9cpm

Go back to Copper Binding Sites List in 9cpm
Copper binding site 4 out of 5 in the Thermus Thermophilus HB27 Laccase (Tth-Lac) Mutant with Partial Deletion of Beta-Hairpin Sequence


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 4 of Thermus Thermophilus HB27 Laccase (Tth-Lac) Mutant with Partial Deletion of Beta-Hairpin Sequence within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cu504

b:13.9
occ:1.00
 ND1 A:HIS97 2.0 9.7 1.0
NE2 A:HIS135 2.0 9.1 1.0
NE2 A:HIS446 2.1 12.0 1.0
O A:HOH938 2.8 36.4 1.0
CE1 A:HIS97 2.9 11.1 1.0
CE1 A:HIS135 2.9 9.2 1.0
CG A:HIS97 3.0 10.7 1.0
CE1 A:HIS446 3.0 12.6 1.0
CD2 A:HIS135 3.0 9.6 1.0
CD2 A:HIS446 3.2 12.3 1.0
CB A:HIS97 3.4 10.6 1.0
CZ2 A:TRP133 3.6 9.6 1.0
CE2 A:TRP133 4.0 9.9 1.0
NE2 A:HIS97 4.0 11.2 1.0
CD2 A:HIS95 4.0 13.2 1.0
CU A:CU502 4.0 15.4 1.0
CD2 A:HIS97 4.1 11.8 1.0
ND1 A:HIS135 4.1 9.2 1.0
NE1 A:TRP133 4.1 11.3 1.0
CG A:HIS135 4.2 8.9 1.0
ND1 A:HIS446 4.2 11.4 1.0
CG A:HIS446 4.3 10.8 1.0
CH2 A:TRP133 4.4 10.1 1.0
NE2 A:HIS396 4.4 10.8 1.0
CD2 A:HIS396 4.6 10.8 1.0
CA A:HIS97 4.6 10.4 1.0
NE2 A:HIS95 4.6 12.3 1.0
O A:HOH652 4.6 30.3 1.0
CD2 A:TRP133 4.9 9.6 1.0
CU A:CU503 5.0 22.0 1.0

Copper binding site 5 out of 5 in 9cpm

Go back to Copper Binding Sites List in 9cpm
Copper binding site 5 out of 5 in the Thermus Thermophilus HB27 Laccase (Tth-Lac) Mutant with Partial Deletion of Beta-Hairpin Sequence


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 5 of Thermus Thermophilus HB27 Laccase (Tth-Lac) Mutant with Partial Deletion of Beta-Hairpin Sequence within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cu505

b:18.8
occ:1.00
 NE2 A:GLN36 1.9 12.0 0.6
NE2 A:GLN36 2.6 13.7 0.5
O A:HOH966 2.7 32.1 1.0
OE1 A:GLN36 2.7 12.4 0.5
CD A:GLN36 3.0 15.7 0.5
CD A:GLN36 3.2 16.6 0.6
CG A:GLN36 4.0 14.7 0.6
OE1 A:GLN36 4.1 14.6 0.6
O A:HOH980 4.1 43.3 1.0
CG A:GLN36 4.5 13.3 0.5

Reference:

B.Miranda-Zaragoza, G.A.Huerta-Miranda, W.I.Garcia-Garcia, E.Hernandez-Alvarez, A.Solano-Peralta, J.Lee, N.Strynadka, M.Miranda-Hernandez, C.Rodriguez-Almazan. Structure-Function Relationship of the Beta-Hairpin of Thermus Thermophilus HB27 Laccase. Int J Mol Sci V. 26 2025.
ISSN: ESSN 1422-0067
PubMed: 39859450
DOI: 10.3390/IJMS26020735
Page generated: Sun Feb 9 06:53:21 2025

Last articles

Zn in 9MJ5
Zn in 9HNW
Zn in 9G0L
Zn in 9FNE
Zn in 9DZN
Zn in 9E0I
Zn in 9D32
Zn in 9DAK
Zn in 8ZXC
Zn in 8ZUF
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy