Atomistry » Copper » PDB 8yk7-9fdl » 8zd5
Atomistry »
  Copper »
    PDB 8yk7-9fdl »
      8zd5 »

Copper in PDB 8zd5: Crystal Structure of E40K Variant of Cu/Zn-Superoxide Dismutase From Dog (Canis Familiaris) in the Holo Form Complexed with 22E1 Fv-Clasp

Enzymatic activity of Crystal Structure of E40K Variant of Cu/Zn-Superoxide Dismutase From Dog (Canis Familiaris) in the Holo Form Complexed with 22E1 Fv-Clasp

All present enzymatic activity of Crystal Structure of E40K Variant of Cu/Zn-Superoxide Dismutase From Dog (Canis Familiaris) in the Holo Form Complexed with 22E1 Fv-Clasp:
1.15.1.1;

Protein crystallography data

The structure of Crystal Structure of E40K Variant of Cu/Zn-Superoxide Dismutase From Dog (Canis Familiaris) in the Holo Form Complexed with 22E1 Fv-Clasp, PDB code: 8zd5 was solved by Y.Shino, Y.Furukawa, N.Muraki, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 48.15 / 2.89
Space group I 1 2 1
Cell size a, b, c (Å), α, β, γ (°) 167.915, 82.213, 187.612, 90, 108.46, 90
R / Rfree (%) 20.1 / 24.9

Other elements in 8zd5:

The structure of Crystal Structure of E40K Variant of Cu/Zn-Superoxide Dismutase From Dog (Canis Familiaris) in the Holo Form Complexed with 22E1 Fv-Clasp also contains other interesting chemical elements:

Zinc (Zn) 4 atoms

Copper Binding Sites:

The binding sites of Copper atom in the Crystal Structure of E40K Variant of Cu/Zn-Superoxide Dismutase From Dog (Canis Familiaris) in the Holo Form Complexed with 22E1 Fv-Clasp (pdb code 8zd5). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 4 binding sites of Copper where determined in the Crystal Structure of E40K Variant of Cu/Zn-Superoxide Dismutase From Dog (Canis Familiaris) in the Holo Form Complexed with 22E1 Fv-Clasp, PDB code: 8zd5:
Jump to Copper binding site number: 1; 2; 3; 4;

Copper binding site 1 out of 4 in 8zd5

Go back to Copper Binding Sites List in 8zd5
Copper binding site 1 out of 4 in the Crystal Structure of E40K Variant of Cu/Zn-Superoxide Dismutase From Dog (Canis Familiaris) in the Holo Form Complexed with 22E1 Fv-Clasp


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of Crystal Structure of E40K Variant of Cu/Zn-Superoxide Dismutase From Dog (Canis Familiaris) in the Holo Form Complexed with 22E1 Fv-Clasp within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cu202

b:89.3
occ:1.00
NE2 A:HIS120 2.0 85.0 1.0
ND1 A:HIS46 2.0 75.1 1.0
NE2 A:HIS48 2.1 76.7 1.0
CD2 A:HIS120 2.9 77.5 1.0
CG A:HIS46 2.9 71.3 1.0
CE1 A:HIS48 3.0 75.5 1.0
CD2 A:HIS48 3.1 74.5 1.0
CE1 A:HIS120 3.1 82.0 1.0
CB A:HIS46 3.1 72.6 1.0
CE1 A:HIS46 3.1 82.5 1.0
NE2 A:HIS63 3.3 94.3 1.0
CD2 A:HIS63 3.8 94.8 1.0
CG A:HIS120 4.0 71.5 1.0
CE1 A:HIS63 4.1 95.9 1.0
CD2 A:HIS46 4.1 74.7 1.0
ND1 A:HIS120 4.1 80.1 1.0
ND1 A:HIS48 4.2 69.5 1.0
NE2 A:HIS46 4.2 73.3 1.0
CB A:VAL118 4.2 67.4 1.0
CG A:HIS48 4.2 72.0 1.0
CA A:HIS46 4.2 76.3 1.0
CG1 A:VAL118 4.2 71.2 1.0
N A:HIS46 4.3 80.1 1.0
O A:VAL118 4.4 67.8 1.0
O A:HIS46 4.5 70.2 1.0
CG A:HIS63 4.7 88.8 1.0
C A:HIS46 4.7 73.2 1.0
ND1 A:HIS63 4.8 85.5 1.0

Copper binding site 2 out of 4 in 8zd5

Go back to Copper Binding Sites List in 8zd5
Copper binding site 2 out of 4 in the Crystal Structure of E40K Variant of Cu/Zn-Superoxide Dismutase From Dog (Canis Familiaris) in the Holo Form Complexed with 22E1 Fv-Clasp


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of Crystal Structure of E40K Variant of Cu/Zn-Superoxide Dismutase From Dog (Canis Familiaris) in the Holo Form Complexed with 22E1 Fv-Clasp within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Cu202

b:78.1
occ:1.00
NE2 D:HIS48 2.0 64.0 1.0
NE2 D:HIS120 2.1 73.4 1.0
ND1 D:HIS46 2.1 77.8 1.0
CG D:HIS46 2.9 62.2 1.0
CD2 D:HIS120 2.9 62.3 1.0
CB D:HIS46 3.0 59.0 1.0
CD2 D:HIS48 3.0 57.5 1.0
CE1 D:HIS48 3.1 64.7 1.0
CE1 D:HIS120 3.1 74.7 1.0
CE1 D:HIS46 3.2 77.7 1.0
NE2 D:HIS63 3.3 85.8 1.0
CD2 D:HIS63 3.8 90.1 1.0
CG D:HIS120 4.0 65.4 1.0
CD2 D:HIS46 4.0 68.2 1.0
CE1 D:HIS63 4.1 89.0 1.0
CA D:HIS46 4.1 59.1 1.0
ND1 D:HIS120 4.1 63.2 1.0
CB D:VAL118 4.2 52.1 1.0
CG D:HIS48 4.2 58.2 1.0
ND1 D:HIS48 4.2 65.0 1.0
N D:HIS46 4.2 63.3 1.0
NE2 D:HIS46 4.2 69.1 1.0
CG1 D:VAL118 4.2 50.5 1.0
O D:HIS46 4.4 78.7 1.0
O D:VAL118 4.4 67.1 1.0
C D:HIS46 4.6 59.5 1.0
CG D:HIS63 4.7 71.3 1.0
ND1 D:HIS63 4.8 75.6 1.0
CG2 D:VAL118 5.0 59.0 1.0

Copper binding site 3 out of 4 in 8zd5

Go back to Copper Binding Sites List in 8zd5
Copper binding site 3 out of 4 in the Crystal Structure of E40K Variant of Cu/Zn-Superoxide Dismutase From Dog (Canis Familiaris) in the Holo Form Complexed with 22E1 Fv-Clasp


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 3 of Crystal Structure of E40K Variant of Cu/Zn-Superoxide Dismutase From Dog (Canis Familiaris) in the Holo Form Complexed with 22E1 Fv-Clasp within 5.0Å range:
probe atom residue distance (Å) B Occ
G:Cu202

b:114.2
occ:1.00
NE2 G:HIS120 2.0 115.0 1.0
NE2 G:HIS48 2.0 91.7 1.0
ND1 G:HIS46 2.1 122.8 1.0
CE1 G:HIS48 2.9 93.2 1.0
CD2 G:HIS120 2.9 107.3 1.0
CG G:HIS46 3.0 108.8 1.0
CE1 G:HIS120 3.0 108.0 1.0
CD2 G:HIS48 3.1 96.2 1.0
CE1 G:HIS46 3.1 125.8 1.0
NE2 G:HIS63 3.1 118.0 1.0
CB G:HIS46 3.2 111.3 1.0
CD2 G:HIS63 3.6 117.7 1.0
CE1 G:HIS63 3.9 131.1 1.0
CG G:HIS120 4.0 100.3 1.0
ND1 G:HIS48 4.1 93.8 1.0
ND1 G:HIS120 4.1 108.8 1.0
CD2 G:HIS46 4.1 114.1 1.0
CG G:HIS48 4.2 102.6 1.0
NE2 G:HIS46 4.2 120.0 1.0
CG1 G:VAL118 4.4 122.8 1.0
CB G:VAL118 4.4 116.0 1.0
CA G:HIS46 4.4 110.0 1.0
N G:HIS46 4.5 104.8 1.0
CG G:HIS63 4.5 109.9 1.0
O G:VAL118 4.6 104.2 1.0
ND1 G:HIS63 4.7 127.0 1.0
O G:HIS46 4.7 122.1 1.0
C G:HIS46 4.9 106.2 1.0

Copper binding site 4 out of 4 in 8zd5

Go back to Copper Binding Sites List in 8zd5
Copper binding site 4 out of 4 in the Crystal Structure of E40K Variant of Cu/Zn-Superoxide Dismutase From Dog (Canis Familiaris) in the Holo Form Complexed with 22E1 Fv-Clasp


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 4 of Crystal Structure of E40K Variant of Cu/Zn-Superoxide Dismutase From Dog (Canis Familiaris) in the Holo Form Complexed with 22E1 Fv-Clasp within 5.0Å range:
probe atom residue distance (Å) B Occ
J:Cu202

b:104.8
occ:1.00
NE2 J:HIS120 2.0 113.5 1.0
ND1 J:HIS46 2.1 96.6 1.0
NE2 J:HIS48 2.1 93.6 1.0
CD2 J:HIS120 2.9 113.6 1.0
CE1 J:HIS48 3.0 84.8 1.0
CG J:HIS46 3.0 86.0 1.0
CE1 J:HIS120 3.0 108.2 1.0
NE2 J:HIS63 3.1 109.4 1.0
CE1 J:HIS46 3.1 95.7 1.0
CD2 J:HIS48 3.2 77.8 1.0
CB J:HIS46 3.2 85.1 1.0
CD2 J:HIS63 3.5 104.1 1.0
CE1 J:HIS63 3.9 118.2 1.0
CG J:HIS120 4.0 111.7 1.0
ND1 J:HIS120 4.1 108.5 1.0
ND1 J:HIS48 4.1 89.5 1.0
CD2 J:HIS46 4.1 84.5 1.0
NE2 J:HIS46 4.2 94.2 1.0
CG J:HIS48 4.2 83.7 1.0
CA J:HIS46 4.4 84.9 1.0
CG1 J:VAL118 4.4 77.5 1.0
CB J:VAL118 4.4 77.9 1.0
CG J:HIS63 4.5 100.0 1.0
N J:HIS46 4.5 98.8 1.0
ND1 J:HIS63 4.6 105.8 1.0
O J:VAL118 4.7 93.6 1.0
O J:HIS46 4.7 99.1 1.0
C J:HIS46 4.9 93.7 1.0

Reference:

Y.Shino, N.Muraki, Y.Furukawa. Disulfide-Mediated Oligomerization of Mutant Cu/Zn-Superoxide Dismutase Associated with Canine Degenerative Myelopathy To Be Published.
Page generated: Tue Dec 10 19:38:13 2024

Last articles

Zn in 9MJ5
Zn in 9HNW
Zn in 9G0L
Zn in 9FNE
Zn in 9DZN
Zn in 9E0I
Zn in 9D32
Zn in 9DAK
Zn in 8ZXC
Zn in 8ZUF
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy