Copper in PDB 8dhy: N-Terminal Fragment of Msba Fused to Gfp in Complex with Copper(II)

All present enzymatic activity of N-Terminal Fragment of Msba Fused to Gfp in Complex with Copper(II):
7.5.2.6;

The structure of N-Terminal Fragment of Msba Fused to Gfp in Complex with Copper(II), PDB code: 8dhy was solved by S.R.Schrecke, T.Zhang, J.Lyu, A.Laganowsky, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	63.08 / 2.15
Space group	I 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	70.61, 108.54, 140.4, 90, 90, 90
R / Rfree (%)	20.2 / 22.8

The binding sites of Copper atom in the N-Terminal Fragment of Msba Fused to Gfp in Complex with Copper(II) (pdb code 8dhy). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total only one binding site of Copper was determined in the N-Terminal Fragment of Msba Fused to Gfp in Complex with Copper(II), PDB code: 8dhy:

Copper binding site 1 out of 1 in the N-Terminal Fragment of Msba Fused to Gfp in Complex with Copper(II)


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of N-Terminal Fragment of Msba Fused to Gfp in Complex with Copper(II) within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu301 b:78.9 occ:1.00 ND1 A:HIS2 2.1 59.5 1.0 N A:HIS2 2.1 67.2 1.0 N A:MET1 2.3 71.3 1.0 O A:HOH401 2.7 64.4 1.0 C A:MET1 2.9 68.2 1.0 O A:HOH437 3.0 69.2 1.0 CG A:HIS2 3.0 57.6 1.0 CA A:MET1 3.0 72.4 1.0 CE1 A:HIS2 3.1 54.9 1.0 CA A:HIS2 3.1 60.1 1.0 CB A:HIS2 3.3 58.5 1.0 N A:ASN3 3.6 61.8 1.0 C A:HIS2 3.9 60.6 1.0 ND2 A:ASN3 4.0 60.8 1.0 O A:MET1 4.0 68.9 1.0 CD2 A:HIS2 4.1 54.9 1.0 NE2 A:HIS2 4.2 57.3 1.0 CB A:MET1 4.4 74.7 1.0 CG A:PRO194 4.4 54.7 1.0 CG A:ASN3 4.9 64.5 1.0 CA A:ASN3 5.0 63.1 1.0

J.Lyu, C.Liu, T.Zhang, S.Schrecke, N.P.Elam, C.Packianathan, G.K.A.Hochberg, D.Russell, M.Zhao, A.Laganowsky. Structural Basis For Lipid and Copper Regulation of the Abc Transporter Msba. Nat Commun V. 13 7291 2022.
ISSN: ESSN 2041-1723
PubMed: 36435815
DOI: 10.1038/S41467-022-34905-2