Copper in PDB 8cc5: Vibrio Cholerae Gbpa (Lpmo Domain)

The structure of Vibrio Cholerae Gbpa (Lpmo Domain), PDB code: 8cc5 was solved by M.Montserrat-Canals, H.V.Sorensen, G.Cordara, U.Krengel, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	47.54 / 1.62
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	75.333, 89.374, 47.497, 90, 90, 90
R / Rfree (%)	17.6 / 21.3

The structure of Vibrio Cholerae Gbpa (Lpmo Domain) also contains other interesting chemical elements:

Zinc	(Zn)	8 atoms
Sodium	(Na)	1 atom

The binding sites of Copper atom in the Vibrio Cholerae Gbpa (Lpmo Domain) (pdb code 8cc5). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 2 binding sites of Copper where determined in the Vibrio Cholerae Gbpa (Lpmo Domain), PDB code: 8cc5:
Jump to Copper binding site number: 1; 2;

Copper binding site 1 out of 2 in the Vibrio Cholerae Gbpa (Lpmo Domain)


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of Vibrio Cholerae Gbpa (Lpmo Domain) within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu303 b:25.8 occ:0.87 ND1 A:HIS24 2.0 24.6 1.0 NE2 A:HIS121 2.0 29.8 1.0 N A:HIS24 2.3 23.2 1.0 CD2 A:HIS121 2.9 28.8 1.0 CG A:HIS24 2.9 22.2 1.0 CE1 A:HIS24 3.0 25.3 1.0 CE1 A:HIS121 3.1 35.2 1.0 CH3 A:ACT302 3.2 44.1 1.0 CA A:HIS24 3.2 22.1 1.0 CB A:HIS24 3.2 24.3 1.0 C A:ACT302 3.5 54.9 1.0 OXT A:ACT302 3.5 37.3 1.0 CZ A:PHE193 3.7 20.1 1.0 CB A:ALA119 3.9 30.6 1.0 CG A:HIS121 4.1 28.7 1.0 CD2 A:HIS24 4.1 26.2 1.0 ND1 A:HIS121 4.1 28.3 1.0 NE2 A:HIS24 4.1 26.6 1.0 CE1 A:PHE193 4.2 22.7 1.0 O A:ALA119 4.3 28.0 1.0 O A:ACT302 4.4 58.6 1.0 CE2 A:PHE193 4.5 19.4 1.0 C A:HIS24 4.5 21.2 1.0 ZN A:ZN304 4.9 24.5 0.7 O A:HIS24 4.9 21.4 1.0

Copper binding site 2 out of 2 in the Vibrio Cholerae Gbpa (Lpmo Domain)


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of Vibrio Cholerae Gbpa (Lpmo Domain) within 5.0Å range: probe atom residue distance (Å) B Occ B:Cu301 b:26.9 occ:0.96 NE2 B:HIS121 1.8 30.1 1.0 ND1 B:HIS24 2.0 24.8 1.0 N B:HIS24 2.2 19.5 1.0 CD2 B:HIS121 2.8 25.9 1.0 CE1 B:HIS121 2.9 38.8 1.0 CG B:HIS24 3.0 23.1 1.0 CE1 B:HIS24 3.0 24.6 1.0 CA B:HIS24 3.1 19.8 1.0 CB B:HIS24 3.3 21.6 1.0 CH3 A:ACT301 3.5 46.1 1.0 CZ B:PHE193 3.6 21.2 1.0 OXT A:ACT301 3.7 35.0 1.0 C A:ACT301 3.7 45.0 1.0 CG B:HIS121 3.9 28.3 1.0 ND1 B:HIS121 4.0 30.3 1.0 CB B:ALA119 4.0 31.7 1.0 CD2 B:HIS24 4.1 25.9 1.0 NE2 B:HIS24 4.1 28.0 1.0 CE1 B:PHE193 4.3 22.1 1.0 O B:ALA119 4.4 25.6 1.0 CE2 B:PHE193 4.4 18.1 1.0 O A:ACT301 4.4 45.2 1.0 C B:HIS24 4.4 22.2 1.0 O B:HIS24 4.8 22.8 1.0 ZN A:ZN305 4.9 25.0 0.7

H.V.Sorensen, M.Montserrat-Canals, J.S.M.Loose, S.Z.Fisher, M.Moulin, M.P.Blakeley, G.Cordara, K.Bjerregaard-Andersen, U.Krengel. Perdeuterated Gbpa Enables Neutron Scattering Experiments of A Lytic Polysaccharide Monooxygenase. Acs Omega V. 8 29101 2023.
ISSN: ESSN 2470-1343
PubMed: 37599915
DOI: 10.1021/ACSOMEGA.3C02168