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Copper in PDB 7ynh: Catalytic Intermediate of Copper Amine Oxidase Determined By Serial Femtosecond X-Ray Crystallography Using A Single-Flow Liquid Jet System

Enzymatic activity of Catalytic Intermediate of Copper Amine Oxidase Determined By Serial Femtosecond X-Ray Crystallography Using A Single-Flow Liquid Jet System

All present enzymatic activity of Catalytic Intermediate of Copper Amine Oxidase Determined By Serial Femtosecond X-Ray Crystallography Using A Single-Flow Liquid Jet System:
1.4.3.21;

Protein crystallography data

The structure of Catalytic Intermediate of Copper Amine Oxidase Determined By Serial Femtosecond X-Ray Crystallography Using A Single-Flow Liquid Jet System, PDB code: 7ynh was solved by T.Murakawa, T.Okajima, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 29.76 / 1.94
Space group C 1 2 1
Cell size a, b, c (Å), α, β, γ (°) 192.51, 64.61, 158.24, 90, 116.86, 90
R / Rfree (%) 17.8 / 21

Other elements in 7ynh:

The structure of Catalytic Intermediate of Copper Amine Oxidase Determined By Serial Femtosecond X-Ray Crystallography Using A Single-Flow Liquid Jet System also contains other interesting chemical elements:

Sodium (Na) 2 atoms

Copper Binding Sites:

The binding sites of Copper atom in the Catalytic Intermediate of Copper Amine Oxidase Determined By Serial Femtosecond X-Ray Crystallography Using A Single-Flow Liquid Jet System (pdb code 7ynh). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 2 binding sites of Copper where determined in the Catalytic Intermediate of Copper Amine Oxidase Determined By Serial Femtosecond X-Ray Crystallography Using A Single-Flow Liquid Jet System, PDB code: 7ynh:
Jump to Copper binding site number: 1; 2;

Copper binding site 1 out of 2 in 7ynh

Go back to Copper Binding Sites List in 7ynh
Copper binding site 1 out of 2 in the Catalytic Intermediate of Copper Amine Oxidase Determined By Serial Femtosecond X-Ray Crystallography Using A Single-Flow Liquid Jet System


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of Catalytic Intermediate of Copper Amine Oxidase Determined By Serial Femtosecond X-Ray Crystallography Using A Single-Flow Liquid Jet System within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cu703

b:24.4
occ:1.00
O A:HOH873 2.0 32.8 1.0
NE2 A:HIS431 2.1 19.7 1.0
ND1 A:HIS592 2.1 22.2 1.0
NE2 A:HIS433 2.1 23.2 1.0
O A:HOH1100 2.4 26.2 0.8
CD2 A:HIS433 2.9 19.8 1.0
CG A:HIS592 3.0 28.2 1.0
CD2 A:HIS431 3.0 22.5 1.0
CE1 A:HIS431 3.0 22.3 1.0
OH A:TYQ382 3.1 23.8 0.4
CE1 A:HIS592 3.1 26.2 1.0
CE1 A:HIS433 3.2 21.0 1.0
CB A:HIS592 3.3 18.6 1.0
O A:HOH1186 3.6 43.8 1.0
CZ A:TYQ382 3.7 23.6 0.4
CG A:HIS433 4.1 20.2 1.0
ND1 A:HIS431 4.1 21.7 1.0
CG A:HIS431 4.2 20.8 1.0
CD2 A:HIS592 4.2 24.2 1.0
NE2 A:HIS592 4.2 27.4 1.0
ND1 A:HIS433 4.2 20.4 1.0
CE1 A:TYQ382 4.3 24.7 0.4
CE2 A:TYQ382 4.4 23.8 0.4
O A:HOH829 4.5 22.9 1.0
N5 A:TYQ382 4.5 28.0 0.4
SD A:MET602 4.8 35.3 1.0
CE A:MET602 4.8 33.7 1.0
OZ A:TYQ382 4.8 26.7 0.6
CA A:HIS592 4.8 22.5 1.0

Copper binding site 2 out of 2 in 7ynh

Go back to Copper Binding Sites List in 7ynh
Copper binding site 2 out of 2 in the Catalytic Intermediate of Copper Amine Oxidase Determined By Serial Femtosecond X-Ray Crystallography Using A Single-Flow Liquid Jet System


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of Catalytic Intermediate of Copper Amine Oxidase Determined By Serial Femtosecond X-Ray Crystallography Using A Single-Flow Liquid Jet System within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Cu703

b:23.7
occ:1.00
O B:HOH1010 1.9 32.0 1.0
NE2 B:HIS431 2.0 18.4 1.0
NE2 B:HIS433 2.1 21.2 1.0
ND1 B:HIS592 2.1 21.0 1.0
O B:HOH1124 2.4 24.6 0.6
CD2 B:HIS433 3.0 17.2 1.0
CE1 B:HIS431 3.0 20.9 1.0
CD2 B:HIS431 3.0 17.4 1.0
CG B:HIS592 3.0 26.5 1.0
CE1 B:HIS592 3.1 25.8 1.0
OH B:TYQ382 3.1 24.8 0.3
CE1 B:HIS433 3.1 19.3 1.0
CB B:HIS592 3.3 18.9 1.0
O B:HOH1217 3.6 42.1 1.0
CZ B:TYQ382 3.8 23.2 0.3
ND1 B:HIS431 4.1 20.6 1.0
CG B:HIS431 4.1 17.8 1.0
CG B:HIS433 4.2 20.2 1.0
NE2 B:HIS592 4.2 23.7 1.0
CD2 B:HIS592 4.2 21.0 1.0
ND1 B:HIS433 4.2 19.8 1.0
CE1 B:TYQ382 4.3 26.7 0.3
CE2 B:TYQ382 4.4 25.0 0.3
O B:HOH805 4.4 24.8 1.0
N5 B:TYQ382 4.5 24.1 0.3
CE B:MET602 4.7 32.5 1.0
SD B:MET602 4.8 32.1 1.0
CA B:HIS592 4.8 16.6 1.0
OZ B:TYQ382 4.9 29.4 0.7

Reference:

T.Murakawa, M.Suzuki, K.Fukui, T.Masuda, M.Sugahara, K.Tono, T.Tanaka, S.Iwata, E.Nango, T.Yano, K.Tanizawa, T.Okajima. Serial Femtosecond X-Ray Crystallography of An Anaerobically Formed Catalytic Intermediate of Copper Amine Oxidase. Acta Crystallogr D Struct V. 78 1428 2022BIOL.
ISSN: ISSN 2059-7983
PubMed: 36458614
DOI: 10.1107/S2059798322010385
Page generated: Wed Jul 31 09:23:35 2024

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