Copper in PDB 7lsj: Cu-Bound Crystal Structure of the Engineered Cyt CB562 Variant, DICYT2 - H63A, Crystallized in the Presence of Cu(II)

The structure of Cu-Bound Crystal Structure of the Engineered Cyt CB562 Variant, DICYT2 - H63A, Crystallized in the Presence of Cu(II), PDB code: 7lsj was solved by T.S.Choi, F.A.Tezcan, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	34.40 / 1.26
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	34.28, 82.72, 38.5, 90, 100.78, 90
R / Rfree (%)	17.5 / 19.2

The structure of Cu-Bound Crystal Structure of the Engineered Cyt CB562 Variant, DICYT2 - H63A, Crystallized in the Presence of Cu(II) also contains other interesting chemical elements:

Calcium	(Ca)	5 atoms
Iron	(Fe)	2 atoms

The binding sites of Copper atom in the Cu-Bound Crystal Structure of the Engineered Cyt CB562 Variant, DICYT2 - H63A, Crystallized in the Presence of Cu(II) (pdb code 7lsj). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total only one binding site of Copper was determined in the Cu-Bound Crystal Structure of the Engineered Cyt CB562 Variant, DICYT2 - H63A, Crystallized in the Presence of Cu(II), PDB code: 7lsj:

Copper binding site 1 out of 1 in the Cu-Bound Crystal Structure of the Engineered Cyt CB562 Variant, DICYT2 - H63A, Crystallized in the Presence of Cu(II)


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of Cu-Bound Crystal Structure of the Engineered Cyt CB562 Variant, DICYT2 - H63A, Crystallized in the Presence of Cu(II) within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu202 b:8.0 occ:1.00 NE2 C:HIS67 2.0 7.3 1.0 NE2 A:HIS67 2.0 9.5 1.0 NE2 C:HIS71 2.1 8.6 1.0 NE2 A:HIS71 2.1 8.4 1.0 NE2 A:HIS97 2.2 7.8 1.0 CE1 A:HIS67 2.9 12.9 1.0 CD2 C:HIS67 2.9 7.7 1.0 CE1 C:HIS71 3.0 11.3 1.0 CE1 A:HIS97 3.0 8.4 1.0 CE1 C:HIS67 3.0 8.3 1.0 CE1 A:HIS71 3.1 8.1 1.0 CD2 A:HIS71 3.1 8.0 1.0 CD2 C:HIS71 3.1 8.0 1.0 CD2 A:HIS67 3.1 7.0 1.0 CD2 A:HIS97 3.2 8.4 1.0 O C:HOH452 4.1 8.3 1.0 ND1 A:HIS67 4.1 13.0 1.0 CG C:HIS67 4.1 7.6 1.0 ND1 C:HIS71 4.1 11.3 1.0 ND1 C:HIS67 4.1 7.2 1.0 ND1 A:HIS97 4.1 8.6 1.0 ND1 A:HIS71 4.2 8.4 1.0 CG A:HIS67 4.2 9.6 1.0 CG C:HIS71 4.2 9.3 1.0 CG A:HIS71 4.2 9.6 1.0 CG A:HIS97 4.2 8.4 1.0 O A:HOH436 4.5 19.4 1.0 CE1 C:HIS97 4.6 9.0 1.0 NE2 C:HIS97 4.9 9.3 1.0

T.S.Choi, F.A.Tezcan. Structure-Guided Metal Selectivity in Malleable Protein Interface Mediated By Single Disulfide Bond To Be Published.