Atomistry » Copper » PDB 7a8v-7ev7 » 7aq2
Atomistry »
  Copper »
    PDB 7a8v-7ev7 »
      7aq2 »

Copper in PDB 7aq2: Pseudomonas Stutzeri Nitrous Oxide Reductase Mutant, H583A

Enzymatic activity of Pseudomonas Stutzeri Nitrous Oxide Reductase Mutant, H583A

All present enzymatic activity of Pseudomonas Stutzeri Nitrous Oxide Reductase Mutant, H583A:
1.7.2.4;

Protein crystallography data

The structure of Pseudomonas Stutzeri Nitrous Oxide Reductase Mutant, H583A, PDB code: 7aq2 was solved by L.Zhang, E.Bill, P.M.H.Kroneck, O.Einsle, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 69.54 / 1.68
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 69.643, 76.501, 108.949, 90, 93.12, 90
R / Rfree (%) 16.3 / 20.1

Other elements in 7aq2:

The structure of Pseudomonas Stutzeri Nitrous Oxide Reductase Mutant, H583A also contains other interesting chemical elements:

Calcium (Ca) 1 atom
Potassium (K) 1 atom
Sodium (Na) 2 atoms
Chlorine (Cl) 3 atoms
Zinc (Zn) 2 atoms

Copper Binding Sites:

The binding sites of Copper atom in the Pseudomonas Stutzeri Nitrous Oxide Reductase Mutant, H583A (pdb code 7aq2). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 6 binding sites of Copper where determined in the Pseudomonas Stutzeri Nitrous Oxide Reductase Mutant, H583A, PDB code: 7aq2:
Jump to Copper binding site number: 1; 2; 3; 4; 5; 6;

Copper binding site 1 out of 6 in 7aq2

Go back to Copper Binding Sites List in 7aq2
Copper binding site 1 out of 6 in the Pseudomonas Stutzeri Nitrous Oxide Reductase Mutant, H583A


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of Pseudomonas Stutzeri Nitrous Oxide Reductase Mutant, H583A within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cu708

b:90.9
occ:0.67
CU1 A:CUA708 0.0 90.9 0.7
SG A:CYS618 2.4 29.6 0.9
SD A:MET629 2.4 34.2 0.7
SG A:CYS622 2.7 52.4 1.0
CU2 A:CUA708 2.7 44.3 0.6
CB A:CYS618 3.1 25.6 1.0
CE A:MET629 3.4 33.3 1.0
CG A:MET629 3.7 34.5 1.0
CB A:CYS622 3.9 51.3 1.0
O A:TRP620 3.9 33.4 1.0
O A:HOH1166 4.0 47.8 1.0
CB A:MET629 4.1 34.9 1.0
ND1 A:HIS626 4.4 48.5 1.0
CA A:CYS618 4.5 18.8 1.0
CB A:ALA583 4.7 19.4 1.0
CA A:ALA583 4.8 20.5 1.0
N A:CYS622 4.9 33.9 1.0
C A:CYS618 5.0 23.4 1.0

Copper binding site 2 out of 6 in 7aq2

Go back to Copper Binding Sites List in 7aq2
Copper binding site 2 out of 6 in the Pseudomonas Stutzeri Nitrous Oxide Reductase Mutant, H583A


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of Pseudomonas Stutzeri Nitrous Oxide Reductase Mutant, H583A within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cu708

b:44.3
occ:0.63
CU2 A:CUA708 0.0 44.3 0.6
ND1 A:HIS626 1.8 48.5 1.0
O A:TRP620 2.6 33.4 1.0
CE1 A:HIS626 2.6 51.0 1.0
SG A:CYS618 2.7 29.6 0.9
CU1 A:CUA708 2.7 90.9 0.7
CG A:HIS626 3.0 45.0 1.0
C A:TRP620 3.3 38.4 1.0
O A:CYS618 3.4 24.1 1.0
CB A:CYS618 3.4 25.6 1.0
CB A:HIS626 3.5 43.0 1.0
SG A:CYS622 3.6 52.4 1.0
NE2 A:HIS626 3.8 48.1 1.0
C A:CYS618 3.8 23.4 1.0
N A:CYS622 3.9 33.9 1.0
CD2 A:HIS626 4.0 49.2 1.0
N A:TRP620 4.0 28.2 1.0
N A:PHE621 4.0 40.9 1.0
CA A:PHE621 4.0 38.2 1.0
CA A:HIS626 4.1 42.1 1.0
O A:HIS626 4.2 36.4 1.0
CA A:CYS618 4.3 18.8 1.0
CA A:TRP620 4.3 33.6 1.0
CB A:CYS622 4.3 51.3 1.0
C A:PHE621 4.3 39.0 1.0
C A:SER619 4.5 33.0 1.0
O A:HOH1075 4.5 46.3 1.0
C A:HIS626 4.5 41.5 1.0
N A:SER619 4.6 25.1 1.0
SD A:MET629 4.7 34.2 0.7
CA A:CYS622 4.7 50.9 1.0
CB A:MET629 4.9 34.9 1.0
CA A:SER619 5.0 23.9 1.0

Copper binding site 3 out of 6 in 7aq2

Go back to Copper Binding Sites List in 7aq2
Copper binding site 3 out of 6 in the Pseudomonas Stutzeri Nitrous Oxide Reductase Mutant, H583A


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 3 of Pseudomonas Stutzeri Nitrous Oxide Reductase Mutant, H583A within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Cu706

b:37.7
occ:0.53
CU1 B:CUZ706 0.0 37.7 0.5
NE2 B:HIS326 2.1 31.0 1.0
NE2 B:HIS382 2.3 43.2 1.0
S1 B:CUZ706 2.5 45.5 0.6
O B:HOH1127 2.6 44.6 1.0
CD2 B:HIS326 3.0 31.6 1.0
CE1 B:HIS326 3.2 31.0 1.0
CU3 B:CUZ706 3.3 27.3 0.6
NE2 B:HIS433 3.3 40.4 1.0
CE1 B:HIS382 3.3 45.1 1.0
CD2 B:HIS382 3.3 40.0 1.0
CU4 B:CUZ706 3.4 39.0 0.5
CD2 B:HIS433 3.5 40.0 1.0
CG B:HIS326 4.2 26.2 1.0
CE1 B:HIS433 4.2 38.8 1.0
ND1 B:HIS326 4.2 33.4 1.0
OD1 B:ASN241 4.3 39.9 1.0
ND1 B:HIS382 4.4 38.0 1.0
CG B:HIS382 4.5 41.0 1.0
CG B:HIS433 4.5 34.5 1.0
CE2 A:PHE621 4.6 41.1 1.0
CU2 B:CUZ706 4.6 32.8 0.3
ND1 B:HIS433 4.9 36.4 1.0

Copper binding site 4 out of 6 in 7aq2

Go back to Copper Binding Sites List in 7aq2
Copper binding site 4 out of 6 in the Pseudomonas Stutzeri Nitrous Oxide Reductase Mutant, H583A


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 4 of Pseudomonas Stutzeri Nitrous Oxide Reductase Mutant, H583A within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Cu706

b:32.8
occ:0.33
CU2 B:CUZ706 0.0 32.8 0.3
S1 B:CUZ706 2.2 45.5 0.6
NE2 B:HIS178 2.3 35.6 1.0
ND1 B:HIS129 2.6 32.2 1.0
CU3 B:CUZ706 2.7 27.3 0.6
CU4 B:CUZ706 2.8 39.0 0.5
CD2 B:HIS178 3.0 34.7 1.0
CG B:HIS129 3.2 26.2 1.0
CE1 B:HIS178 3.3 35.7 1.0
O A:HOH1137 3.4 42.3 1.0
CB B:HIS129 3.4 21.4 1.0
CE1 B:HIS129 3.5 28.3 1.0
NE2 B:HIS130 3.5 25.1 1.0
CE1 B:HIS130 3.6 23.5 1.0
ND1 B:HIS494 3.8 56.8 1.0
CG B:HIS178 4.2 31.7 1.0
CD2 B:HIS129 4.2 27.6 1.0
ND1 B:HIS178 4.3 36.3 1.0
CG B:HIS494 4.3 51.4 1.0
NE2 B:HIS129 4.3 29.8 1.0
CE1 B:HIS494 4.3 54.5 1.0
CD2 B:HIS130 4.6 28.4 1.0
CB B:HIS494 4.6 37.9 1.0
CU1 B:CUZ706 4.6 37.7 0.5
ND1 B:HIS130 4.7 24.1 1.0
NE2 B:HIS433 4.8 40.4 1.0
CA B:HIS129 4.8 19.7 1.0
SD A:MET627 5.0 31.8 0.6

Copper binding site 5 out of 6 in 7aq2

Go back to Copper Binding Sites List in 7aq2
Copper binding site 5 out of 6 in the Pseudomonas Stutzeri Nitrous Oxide Reductase Mutant, H583A


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 5 of Pseudomonas Stutzeri Nitrous Oxide Reductase Mutant, H583A within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Cu706

b:27.3
occ:0.56
CU3 B:CUZ706 0.0 27.3 0.6
NE2 B:HIS433 2.2 40.4 1.0
S1 B:CUZ706 2.2 45.5 0.6
NE2 B:HIS130 2.2 25.1 1.0
CU2 B:CUZ706 2.7 32.8 0.3
CU4 B:CUZ706 2.8 39.0 0.5
CE1 B:HIS433 3.1 38.8 1.0
CD2 B:HIS130 3.1 28.4 1.0
CD2 B:HIS433 3.2 40.0 1.0
CE1 B:HIS130 3.3 23.5 1.0
CU1 B:CUZ706 3.3 37.7 0.5
ND2 B:ASN241 3.8 31.1 1.0
O B:HOH844 4.0 43.9 1.0
NE2 B:HIS382 4.1 43.2 1.0
NE2 B:HIS178 4.2 35.6 1.0
ND1 B:HIS433 4.2 36.4 1.0
CG B:HIS130 4.3 28.1 1.0
CB B:HIS129 4.3 21.4 1.0
CE1 B:HIS382 4.3 45.1 1.0
CG B:HIS433 4.3 34.5 1.0
ND1 B:HIS130 4.3 24.1 1.0
CB B:HIS494 4.4 37.9 1.0
O B:HOH1127 4.4 44.6 1.0
CE1 B:HIS178 4.4 35.7 1.0
NE2 B:HIS326 4.7 31.0 1.0
CG B:ASN241 4.7 33.4 1.0
OD1 B:ASN241 4.7 39.9 1.0
ND1 B:HIS129 4.8 32.2 1.0
ND1 B:HIS494 4.9 56.8 1.0
CG B:HIS129 4.9 26.2 1.0
O B:HIS129 5.0 23.6 1.0
CG B:HIS494 5.0 51.4 1.0

Copper binding site 6 out of 6 in 7aq2

Go back to Copper Binding Sites List in 7aq2
Copper binding site 6 out of 6 in the Pseudomonas Stutzeri Nitrous Oxide Reductase Mutant, H583A


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 6 of Pseudomonas Stutzeri Nitrous Oxide Reductase Mutant, H583A within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Cu706

b:39.0
occ:0.54
CU4 B:CUZ706 0.0 39.0 0.5
S1 B:CUZ706 2.4 45.5 0.6
ND1 B:HIS494 2.5 56.8 1.0
O B:HOH1127 2.7 44.6 1.0
CU3 B:CUZ706 2.8 27.3 0.6
CU2 B:CUZ706 2.8 32.8 0.3
CB B:HIS494 2.9 37.9 1.0
CG B:HIS494 3.1 51.4 1.0
CU1 B:CUZ706 3.4 37.7 0.5
CD2 B:HIS433 3.5 40.0 1.0
NE2 B:HIS433 3.6 40.4 1.0
CE1 B:HIS494 3.7 54.5 1.0
O A:HOH1137 3.8 42.3 1.0
ND1 B:HIS129 4.0 32.2 1.0
CA B:HIS494 4.1 30.2 1.0
NZ B:LYS454 4.3 37.4 1.0
CD2 B:HIS494 4.3 54.7 1.0
CE2 A:PHE621 4.4 41.1 1.0
CD2 A:PHE621 4.6 40.9 1.0
NE2 B:HIS494 4.6 52.1 1.0
NE2 B:HIS130 4.7 25.1 1.0
CB B:HIS129 4.7 21.4 1.0
C B:HIS494 4.7 24.0 1.0
CG B:HIS129 4.8 26.2 1.0
O B:HOH839 4.8 24.3 1.0
CG B:HIS433 4.8 34.5 1.0
CE1 B:HIS433 4.9 38.8 1.0
NE2 B:HIS178 4.9 35.6 1.0
O B:HIS494 4.9 25.9 1.0
CE1 B:HIS129 4.9 28.3 1.0
CE B:LYS454 4.9 41.5 1.0

Reference:

L.Zhang, E.Bill, P.M.H.Kroneck, O.Einsle. Histidine-Gated Proton-Coupled Electron Transfer to the Cu A Site of Nitrous Oxide Reductase. J.Am.Chem.Soc. 2020.
ISSN: ESSN 1520-5126
PubMed: 33377777
DOI: 10.1021/JACS.0C10057
Page generated: Mon Jul 14 07:47:01 2025

Last articles

Fe in 2YXO
Fe in 2YRS
Fe in 2YXC
Fe in 2YNM
Fe in 2YVJ
Fe in 2YP1
Fe in 2YU2
Fe in 2YU1
Fe in 2YQB
Fe in 2YOO
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy