Copper in PDB 6rl0: Recombinant Pseudomonas Stutzeri Nitrous Oxide Reductase, Form I

All present enzymatic activity of Recombinant Pseudomonas Stutzeri Nitrous Oxide Reductase, Form I:
1.7.2.4;

The structure of Recombinant Pseudomonas Stutzeri Nitrous Oxide Reductase, Form I, PDB code: 6rl0 was solved by L.Zhang, A.Wuest, B.Prasser, C.Mueller, O.Einsle, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	48.91 / 1.78
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	108.916, 73.372, 136.342, 90.00, 95.07, 90.00
R / Rfree (%)	15.7 / 19.2

The structure of Recombinant Pseudomonas Stutzeri Nitrous Oxide Reductase, Form I also contains other interesting chemical elements:

Potassium	(K)	4 atoms
Calcium	(Ca)	4 atoms
Chlorine	(Cl)	4 atoms
Sodium	(Na)	4 atoms

Pages:

>>> Page 1 <<< Page 2, Binding sites: 11 - 20; Page 3, Binding sites: 21 - 24;

Binding sites:

The binding sites of Copper atom in the Recombinant Pseudomonas Stutzeri Nitrous Oxide Reductase, Form I (pdb code 6rl0). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 24 binding sites of Copper where determined in the Recombinant Pseudomonas Stutzeri Nitrous Oxide Reductase, Form I, PDB code: 6rl0:
Jump to Copper binding site number: 1; 2; 3; 4; 5; 6; 7; 8; 9; 10;

Copper binding site 1 out of 24 in the Recombinant Pseudomonas Stutzeri Nitrous Oxide Reductase, Form I


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of Recombinant Pseudomonas Stutzeri Nitrous Oxide Reductase, Form I within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu704 b:50.6 occ:0.58 CU1 A:CUK704 0.0 50.6 0.6 NE2 A:HIS382 2.1 21.7 0.5 NE2 A:HIS326 2.2 31.4 1.0 S1 A:CUK704 2.4 44.0 0.6 S2 A:CUK704 2.6 46.7 0.3 CD2 A:HIS382 3.0 21.4 0.5 CE1 A:HIS382 3.1 21.2 0.5 CE1 A:HIS326 3.1 33.4 1.0 CU3 A:CUK704 3.1 26.7 0.5 NE2 A:HIS433 3.2 35.0 1.0 CD2 A:HIS326 3.3 27.4 1.0 CD2 A:HIS433 3.4 36.1 1.0 CU4 A:CUK704 3.4 39.6 0.6 CE1 A:HIS433 3.8 33.0 1.0 CG A:HIS433 4.1 28.2 1.0 ND1 A:HIS382 4.2 21.1 0.5 CG A:HIS382 4.2 20.6 0.5 ND1 A:HIS326 4.3 29.7 1.0 CE D:MET627 4.3 49.1 1.0 CG A:HIS326 4.4 26.3 1.0 ND2 A:ASN241 4.4 27.4 1.0 ND1 A:HIS433 4.4 29.4 1.0 CU2 A:CUK704 4.5 37.5 0.5 N A:HIS433 4.8 19.3 1.0 C A:GLY432 5.0 21.2 1.0

Copper binding site 2 out of 24 in the Recombinant Pseudomonas Stutzeri Nitrous Oxide Reductase, Form I


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of Recombinant Pseudomonas Stutzeri Nitrous Oxide Reductase, Form I within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu704 b:37.5 occ:0.52 CU2 A:CUK704 0.0 37.5 0.5 NE2 A:HIS178 2.0 30.4 1.0 S1 A:CUK704 2.2 44.0 0.6 ND1 A:HIS129 2.5 26.6 1.0 CU4 A:CUK704 2.7 39.6 0.6 CU3 A:CUK704 2.8 26.7 0.5 CD2 A:HIS178 2.9 29.9 1.0 CE1 A:HIS178 3.0 32.5 1.0 CG A:HIS129 3.2 22.1 1.0 O D:HOH1044 3.3 34.6 1.0 CE1 A:HIS129 3.3 24.1 1.0 NE2 A:HIS130 3.4 18.2 1.0 CB A:HIS129 3.5 19.6 1.0 CE1 A:HIS130 3.5 17.7 1.0 CD2 A:HIS494 3.9 49.5 1.0 CG A:HIS178 4.1 27.8 1.0 ND1 A:HIS178 4.1 30.0 1.0 CD2 A:HIS129 4.2 22.8 1.0 CE D:MET627 4.2 49.1 1.0 NE2 A:HIS129 4.3 24.1 1.0 CG A:HIS494 4.3 43.0 1.0 CU1 A:CUK704 4.5 50.6 0.6 CD2 A:HIS130 4.5 17.4 1.0 NE2 A:HIS494 4.6 47.3 1.0 ND1 A:HIS130 4.6 17.4 1.0 CB A:HIS494 4.6 32.8 1.0 NE2 A:HIS433 4.7 35.0 1.0 CA A:HIS129 4.8 17.4 1.0 S2 A:CUK704 4.9 46.7 0.3 SD D:MET627 5.0 40.7 0.6

Copper binding site 3 out of 24 in the Recombinant Pseudomonas Stutzeri Nitrous Oxide Reductase, Form I


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 3 of Recombinant Pseudomonas Stutzeri Nitrous Oxide Reductase, Form I within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu704 b:26.7 occ:0.52 CU3 A:CUK704 0.0 26.7 0.5 NE2 A:HIS433 2.0 35.0 1.0 NE2 A:HIS130 2.1 18.2 1.0 S1 A:CUK704 2.2 44.0 0.6 CU2 A:CUK704 2.8 37.5 0.5 CU4 A:CUK704 2.8 39.6 0.6 CD2 A:HIS130 2.9 17.4 1.0 CE1 A:HIS433 3.0 33.0 1.0 CD2 A:HIS433 3.0 36.1 1.0 CU1 A:CUK704 3.1 50.6 0.6 CE1 A:HIS130 3.1 17.7 1.0 NE2 A:HIS382 3.9 21.7 0.5 OD1 A:ASN241 4.0 31.5 1.0 ND1 A:HIS433 4.1 29.4 1.0 CG A:HIS130 4.1 16.7 1.0 NE2 A:HIS178 4.1 30.4 1.0 CG A:HIS433 4.1 28.2 1.0 S2 A:CUK704 4.1 46.7 0.3 ND1 A:HIS130 4.2 17.4 1.0 CB A:HIS129 4.2 19.6 1.0 CB A:HIS494 4.2 32.8 1.0 CE1 A:HIS382 4.2 21.2 0.5 O A:HOH1075 4.3 34.6 1.0 CE1 A:HIS178 4.4 32.5 1.0 ND1 A:HIS129 4.6 26.6 1.0 NE2 A:HIS326 4.6 31.4 1.0 CG A:ASN241 4.7 25.7 1.0 ND2 A:ASN241 4.7 27.4 1.0 CG A:HIS129 4.8 22.1 1.0 O A:HIS129 4.8 19.7 1.0 CG A:HIS494 4.8 43.0 1.0 CE1 A:HIS326 4.9 33.4 1.0 CD2 A:HIS494 4.9 49.5 1.0

Copper binding site 4 out of 24 in the Recombinant Pseudomonas Stutzeri Nitrous Oxide Reductase, Form I


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 4 of Recombinant Pseudomonas Stutzeri Nitrous Oxide Reductase, Form I within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu704 b:39.6 occ:0.59 CU4 A:CUK704 0.0 39.6 0.6 S1 A:CUK704 2.4 44.0 0.6 CD2 A:HIS494 2.5 49.5 1.0 S2 A:CUK704 2.5 46.7 0.3 CU2 A:CUK704 2.7 37.5 0.5 CU3 A:CUK704 2.8 26.7 0.5 CG A:HIS494 2.9 43.0 1.0 CB A:HIS494 2.9 32.8 1.0 CU1 A:CUK704 3.4 50.6 0.6 O D:HOH1044 3.6 34.6 1.0 ND1 A:HIS129 3.6 26.6 1.0 CD2 A:HIS433 3.7 36.1 1.0 NE2 A:HIS494 3.7 47.3 1.0 NE2 A:HIS433 3.8 35.0 1.0 ND1 A:HIS494 4.2 48.7 1.0 CA A:HIS494 4.2 28.3 1.0 NZ A:LYS454 4.3 31.9 1.0 CE D:MET627 4.4 49.1 1.0 CE1 A:HIS129 4.5 24.1 1.0 CG A:HIS129 4.5 22.1 1.0 NE2 A:HIS130 4.6 18.2 1.0 CE1 A:HIS494 4.6 39.4 1.0 NE2 A:HIS178 4.6 30.4 1.0 CB A:HIS129 4.6 19.6 1.0 CE2 D:PHE621 4.8 37.4 1.0 CD2 D:PHE621 4.8 36.9 1.0 C A:HIS494 4.9 21.4 1.0 O A:HOH987 4.9 23.5 1.0

Copper binding site 5 out of 24 in the Recombinant Pseudomonas Stutzeri Nitrous Oxide Reductase, Form I


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 5 of Recombinant Pseudomonas Stutzeri Nitrous Oxide Reductase, Form I within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu705 b:29.1 occ:1.00 CU1 A:CUA705 0.0 29.1 1.0 ND1 A:HIS626 2.0 23.6 1.0 SG A:CYS622 2.4 22.7 0.9 CU2 A:CUA705 2.4 25.4 1.0 O A:TRP620 2.5 19.6 1.0 SG A:CYS618 2.6 18.0 0.8 CE1 A:HIS626 3.0 23.3 1.0 CG A:HIS626 3.1 23.7 1.0 CB A:CYS622 3.3 23.6 1.0 N A:CYS622 3.4 22.3 1.0 C A:TRP620 3.5 22.2 1.0 CB A:HIS626 3.5 24.8 1.0 CB A:CYS618 3.6 19.7 1.0 CA A:PHE621 3.9 23.9 1.0 CA A:CYS622 3.9 24.3 1.0 CA A:HIS626 4.0 24.6 1.0 C A:PHE621 4.1 24.3 1.0 NE2 A:HIS626 4.1 23.9 1.0 O A:CYS618 4.1 21.7 1.0 N A:PHE621 4.1 20.4 1.0 CD2 A:HIS626 4.2 23.8 1.0 SD A:MET629 4.3 22.6 0.9 O A:HIS626 4.3 21.5 1.0 N A:TRP620 4.3 20.1 1.0 C A:CYS618 4.4 19.4 1.0 CA A:TRP620 4.5 19.9 1.0 C A:HIS626 4.6 24.6 1.0 CA A:CYS618 4.6 19.1 1.0 CB A:MET629 4.6 21.8 1.0 C A:CYS622 4.9 25.4 1.0 O A:HOH924 4.9 26.7 1.0 CG A:MET629 4.9 21.5 1.0 ND1 A:HIS583 5.0 40.1 1.0

Copper binding site 6 out of 24 in the Recombinant Pseudomonas Stutzeri Nitrous Oxide Reductase, Form I


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 6 of Recombinant Pseudomonas Stutzeri Nitrous Oxide Reductase, Form I within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu705 b:25.4 occ:1.00 CU2 A:CUA705 0.0 25.4 1.0 SG A:CYS618 2.3 18.0 0.8 SG A:CYS622 2.4 22.7 0.9 SD A:MET629 2.4 22.6 0.9 CU1 A:CUA705 2.4 29.1 1.0 ND1 A:HIS583 3.0 40.1 1.0 CB A:CYS618 3.2 19.7 1.0 CE A:MET629 3.2 21.9 1.0 CB A:CYS622 3.3 23.6 1.0 CG A:MET629 3.7 21.5 1.0 CE1 A:HIS583 3.8 33.3 1.0 O A:TRP620 3.8 19.6 1.0 CG A:HIS583 3.9 30.0 1.0 CB A:HIS583 4.1 23.6 1.0 CB A:MET629 4.1 21.8 1.0 CA A:HIS583 4.3 20.9 1.0 ND1 A:HIS626 4.4 23.6 1.0 CA A:CYS618 4.6 19.1 1.0 CA A:CYS622 4.7 24.3 1.0 O A:SER582 4.7 20.4 1.0 N A:CYS622 4.9 22.3 1.0 NE2 A:HIS583 4.9 30.7 1.0 N A:GLY584 4.9 19.2 1.0 CD2 A:HIS583 5.0 31.5 1.0

Copper binding site 7 out of 24 in the Recombinant Pseudomonas Stutzeri Nitrous Oxide Reductase, Form I


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 7 of Recombinant Pseudomonas Stutzeri Nitrous Oxide Reductase, Form I within 5.0Å range: probe atom residue distance (Å) B Occ B:Cu704 b:48.1 occ:0.53 CU1 B:CUK704 0.0 48.1 0.5 NE2 B:HIS382 2.1 22.8 0.5 NE2 B:HIS326 2.1 29.1 1.0 S1 B:CUK704 2.4 43.1 0.6 S2 B:CUK704 2.6 45.9 0.3 CD2 B:HIS382 3.0 22.1 0.5 CE1 B:HIS382 3.0 21.9 0.5 CE1 B:HIS326 3.1 32.6 1.0 CU3 B:CUK704 3.1 28.7 0.5 NE2 B:HIS433 3.1 42.7 1.0 CD2 B:HIS326 3.2 25.5 1.0 CU4 B:CUK704 3.4 46.0 0.7 CD2 B:HIS433 3.4 37.8 1.0 CE1 B:HIS433 3.8 36.6 1.0 CG B:HIS433 4.1 33.3 1.0 ND1 B:HIS382 4.2 21.1 0.5 CG B:HIS382 4.2 20.6 0.5 ND1 B:HIS326 4.2 27.4 1.0 CG B:HIS326 4.3 25.0 1.0 ND1 B:HIS433 4.4 34.7 1.0 ND2 B:ASN241 4.4 28.1 1.0 CU2 B:CUK704 4.6 36.3 0.5 CE2 C:PHE621 4.9 36.1 1.0 N B:HIS433 4.9 21.4 1.0 C B:GLY432 5.0 26.4 1.0

Copper binding site 8 out of 24 in the Recombinant Pseudomonas Stutzeri Nitrous Oxide Reductase, Form I


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 8 of Recombinant Pseudomonas Stutzeri Nitrous Oxide Reductase, Form I within 5.0Å range: probe atom residue distance (Å) B Occ B:Cu704 b:36.3 occ:0.52 CU2 B:CUK704 0.0 36.3 0.5 NE2 B:HIS178 2.1 30.0 1.0 S1 B:CUK704 2.2 43.1 0.6 CD2 B:HIS129 2.5 26.6 1.0 CU4 B:CUK704 2.8 46.0 0.7 CU3 B:CUK704 2.8 28.7 0.5 CD2 B:HIS178 2.9 31.3 1.0 CG B:HIS129 3.0 23.1 1.0 CE1 B:HIS178 3.1 33.7 1.0 O C:HOH1099 3.3 39.9 1.0 NE2 B:HIS130 3.3 19.4 1.0 CB B:HIS129 3.3 20.3 1.0 CE1 B:HIS130 3.5 18.6 1.0 NE2 B:HIS129 3.6 26.6 1.0 CD2 B:HIS494 3.9 45.7 1.0 CG B:HIS178 4.1 27.9 1.0 ND1 B:HIS129 4.1 25.6 1.0 ND1 B:HIS178 4.1 32.1 1.0 CG B:HIS494 4.3 42.3 1.0 CE1 B:HIS129 4.4 24.2 1.0 CD2 B:HIS130 4.4 18.9 1.0 ND1 B:HIS130 4.5 18.3 1.0 CU1 B:CUK704 4.6 48.1 0.5 CB B:HIS494 4.6 34.7 1.0 NE2 B:HIS433 4.6 42.7 1.0 NE2 B:HIS494 4.6 43.8 1.0 CA B:HIS129 4.7 19.6 1.0

Copper binding site 9 out of 24 in the Recombinant Pseudomonas Stutzeri Nitrous Oxide Reductase, Form I


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 9 of Recombinant Pseudomonas Stutzeri Nitrous Oxide Reductase, Form I within 5.0Å range: probe atom residue distance (Å) B Occ B:Cu704 b:28.7 occ:0.52 CU3 B:CUK704 0.0 28.7 0.5 NE2 B:HIS433 2.0 42.7 1.0 NE2 B:HIS130 2.0 19.4 1.0 S1 B:CUK704 2.2 43.1 0.6 CU2 B:CUK704 2.8 36.3 0.5 CD2 B:HIS130 2.9 18.9 1.0 CU4 B:CUK704 2.9 46.0 0.7 CE1 B:HIS433 2.9 36.6 1.0 CD2 B:HIS433 3.0 37.8 1.0 CE1 B:HIS130 3.1 18.6 1.0 CU1 B:CUK704 3.1 48.1 0.5 NE2 B:HIS382 3.9 22.8 0.5 OD1 B:ASN241 4.0 38.2 1.0 ND1 B:HIS433 4.0 34.7 1.0 CG B:HIS130 4.1 19.2 1.0 NE2 B:HIS178 4.1 30.0 1.0 CG B:HIS433 4.1 33.3 1.0 O B:HOH1026 4.1 39.2 1.0 ND1 B:HIS130 4.2 18.3 1.0 CB B:HIS129 4.2 20.3 1.0 CE1 B:HIS382 4.2 21.9 0.5 S2 B:CUK704 4.2 45.9 0.3 CB B:HIS494 4.3 34.7 1.0 CE1 B:HIS178 4.4 33.7 1.0 NE2 B:HIS326 4.6 29.1 1.0 CG B:ASN241 4.7 26.4 1.0 ND2 B:ASN241 4.7 28.1 1.0 CD2 B:HIS129 4.7 26.6 1.0 CG B:HIS129 4.8 23.1 1.0 O B:HIS129 4.8 20.5 1.0 CG B:HIS494 4.9 42.3 1.0 CE1 B:HIS326 5.0 32.6 1.0

Copper binding site 10 out of 24 in the Recombinant Pseudomonas Stutzeri Nitrous Oxide Reductase, Form I


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 10 of Recombinant Pseudomonas Stutzeri Nitrous Oxide Reductase, Form I within 5.0Å range: probe atom residue distance (Å) B Occ B:Cu704 b:46.0 occ:0.71 CU4 B:CUK704 0.0 46.0 0.7 S1 B:CUK704 2.3 43.1 0.6 S2 B:CUK704 2.5 45.9 0.3 CD2 B:HIS494 2.5 45.7 1.0 CU2 B:CUK704 2.8 36.3 0.5 CU3 B:CUK704 2.9 28.7 0.5 CG B:HIS494 3.0 42.3 1.0 CB B:HIS494 3.0 34.7 1.0 CU1 B:CUK704 3.4 48.1 0.5 O C:HOH1099 3.5 39.9 1.0 CD2 B:HIS433 3.6 37.8 1.0 NE2 B:HIS433 3.7 42.7 1.0 CD2 B:HIS129 3.8 26.6 1.0 NE2 B:HIS494 3.8 43.8 1.0 CA B:HIS494 4.2 30.7 1.0 ND1 B:HIS494 4.3 47.2 1.0 NZ B:LYS454 4.3 30.0 1.0 CG B:HIS129 4.6 23.1 1.0 NE2 B:HIS130 4.6 19.4 1.0 CE2 C:PHE621 4.6 36.1 1.0 CE1 B:HIS494 4.6 35.5 1.0 CB B:HIS129 4.6 20.3 1.0 NE2 B:HIS178 4.7 30.0 1.0 CD2 C:PHE621 4.7 34.2 1.0 NE2 B:HIS129 4.9 26.6 1.0 C B:HIS494 4.9 26.0 1.0 CG B:HIS433 4.9 33.3 1.0 CE1 B:HIS433 5.0 36.6 1.0 O B:HOH964 5.0 26.3 1.0

L.Zhang, A.Wust, B.Prasser, C.Muller, O.Einsle. Functional Assembly of Nitrous Oxide Reductase Provides Insights Into Copper Site Maturation. Proc.Natl.Acad.Sci.Usa V. 116 12822 2019.
ISSN: ESSN 1091-6490
PubMed: 31189605
DOI: 10.1073/PNAS.1903819116