Copper in PDB 6quh: Ghk Tagged Gfp Variant Crystal Form II at 1.34A Wavelength

The structure of Ghk Tagged Gfp Variant Crystal Form II at 1.34A Wavelength, PDB code: 6quh was solved by T.Huyton, D.Gorlich, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	71.16 / 1.50
Space group	P 2 21 21
Cell size a, b, c (Å), α, β, γ (°)	53.476, 99.307, 102.011, 90.00, 90.00, 90.00
R / Rfree (%)	15.9 / 21.9

The structure of Ghk Tagged Gfp Variant Crystal Form II at 1.34A Wavelength also contains other interesting chemical elements:

Calcium	(Ca)	1 atom
Chlorine	(Cl)	18 atoms
Sodium	(Na)	11 atoms

The binding sites of Copper atom in the Ghk Tagged Gfp Variant Crystal Form II at 1.34A Wavelength (pdb code 6quh). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 6 binding sites of Copper where determined in the Ghk Tagged Gfp Variant Crystal Form II at 1.34A Wavelength, PDB code: 6quh:
Jump to Copper binding site number: 1; 2; 3; 4; 5; 6;

Copper binding site 1 out of 6 in the Ghk Tagged Gfp Variant Crystal Form II at 1.34A Wavelength


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of Ghk Tagged Gfp Variant Crystal Form II at 1.34A Wavelength within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu304 b:20.5 occ:0.00 O A:HOH580 2.0 31.1 1.0 ND1 A:HIS1 2.1 23.9 1.0 N A:HIS1 2.3 23.7 1.0 N A:GLY0 2.4 85.8 1.0 CG A:HIS1 2.9 26.1 1.0 C A:GLY0 2.9 28.4 1.0 CA A:GLY0 2.9 27.7 1.0 CE1 A:HIS1 3.1 21.9 1.0 CB A:HIS1 3.2 23.2 1.0 CA A:HIS1 3.3 21.8 1.0 CD2 A:HIS1 4.1 25.2 1.0 O A:GLY0 4.1 30.3 1.0 NE2 A:HIS1 4.1 25.7 1.0 O A:HIS1 4.2 25.1 1.0 C A:HIS1 4.2 16.5 1.0

Copper binding site 2 out of 6 in the Ghk Tagged Gfp Variant Crystal Form II at 1.34A Wavelength


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of Ghk Tagged Gfp Variant Crystal Form II at 1.34A Wavelength within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu305 b:39.6 occ:0.40 NE2 A:HIS25 1.9 37.4 1.0 OE1 A:GLU132 2.3 47.9 1.0 OE2 A:GLU132 2.6 43.6 1.0 CD A:GLU132 2.8 39.7 1.0 CE1 A:HIS25 2.9 42.8 1.0 CD2 A:HIS25 3.0 30.2 1.0 O A:HOH442 4.0 40.5 1.0 ND1 A:HIS25 4.0 45.5 1.0 CG A:HIS25 4.1 34.3 1.0 O A:HOH465 4.2 45.2 1.0 CG A:GLU132 4.3 39.8 1.0 CD1 A:LEU137 4.3 29.4 1.0 O A:ASN23 4.7 27.2 1.0 CB A:ASN23 4.8 22.9 1.0 CD2 A:LEU137 5.0 29.0 1.0

Copper binding site 3 out of 6 in the Ghk Tagged Gfp Variant Crystal Form II at 1.34A Wavelength


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 3 of Ghk Tagged Gfp Variant Crystal Form II at 1.34A Wavelength within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu306 b:32.6 occ:0.30 O A:HOH425 2.3 32.3 1.0 OE2 A:GLU142 2.3 30.0 1.0 O A:HOH634 2.3 36.7 1.0 O A:HOH409 2.3 43.1 1.0 O A:HOH540 2.4 37.9 1.0 O A:HOH588 2.4 46.8 1.0 OE1 A:GLU142 2.6 30.2 1.0 CD A:GLU142 2.7 25.9 1.0 O A:VAL171 4.1 22.6 1.0 CG A:GLU142 4.1 18.8 1.0 O C:HOH630 4.2 47.4 1.0 O A:HOH505 4.5 45.2 1.0 O C:HOH645 4.5 62.2 1.0 NA A:NA322 4.6 30.5 0.7 CA A:GLY174 4.7 22.6 1.0 CB A:ASN170 4.8 17.0 1.0 ND2 A:ASN170 4.8 20.0 1.0 O A:GLU172 4.8 26.1 1.0 O A:HOH609 4.8 82.1 1.0 N A:GLY174 4.9 23.1 1.0 O A:HOH421 4.9 38.1 1.0

Copper binding site 4 out of 6 in the Ghk Tagged Gfp Variant Crystal Form II at 1.34A Wavelength


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 4 of Ghk Tagged Gfp Variant Crystal Form II at 1.34A Wavelength within 5.0Å range: probe atom residue distance (Å) B Occ C:Cu304 b:19.3 occ:0.00 N C:GLY0 1.7 89.8 1.0 N C:HIS1 2.0 0.4 1.0 ND1 C:HIS1 2.2 34.0 1.0 C C:GLY0 2.7 63.7 1.0 CA C:GLY0 2.7 38.2 1.0 O C:HOH464 2.8 27.1 1.0 CG C:HIS1 3.0 23.3 1.0 CB C:HIS1 3.0 23.5 1.0 CA C:HIS1 3.0 25.7 1.0 CE1 C:HIS1 3.4 22.5 1.0 O C:HOH508 3.5 38.8 1.0 N C:LYS2 3.8 19.8 1.0 C C:HIS1 3.9 19.2 1.0 O C:GLY0 4.0 33.1 1.0 CD2 C:HIS1 4.2 24.4 1.0 NE2 C:HIS1 4.4 28.0 1.0 O C:HOH493 4.4 47.7 1.0 CG C:LYS2 4.8 20.3 1.0

Copper binding site 5 out of 6 in the Ghk Tagged Gfp Variant Crystal Form II at 1.34A Wavelength


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 5 of Ghk Tagged Gfp Variant Crystal Form II at 1.34A Wavelength within 5.0Å range: probe atom residue distance (Å) B Occ C:Cu305 b:29.7 occ:0.91 O C:HOH405 1.8 33.5 1.0 OE1 C:GLU132 1.8 25.1 1.0 NE2 C:HIS25 2.4 25.1 1.0 CD C:GLU132 2.5 24.2 1.0 OE2 C:GLU132 2.8 28.0 1.0 CD2 C:HIS25 3.3 20.8 1.0 CE1 C:HIS25 3.3 27.6 1.0 O C:HOH590 3.9 40.4 1.0 O C:HOH456 3.9 31.0 1.0 CG C:GLU132 4.0 22.9 1.0 CD1 C:LEU137 4.3 17.5 1.0 ND1 C:HIS25 4.4 25.4 1.0 CG C:HIS25 4.4 19.3 1.0 CB C:GLU132 4.6 20.4 1.0 CB C:ASN23 4.9 17.7 1.0 O C:ASN23 5.0 24.5 1.0

Copper binding site 6 out of 6 in the Ghk Tagged Gfp Variant Crystal Form II at 1.34A Wavelength


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 6 of Ghk Tagged Gfp Variant Crystal Form II at 1.34A Wavelength within 5.0Å range: probe atom residue distance (Å) B Occ C:Cu306 b:33.8 occ:0.67 NE2 C:HIS77 2.0 25.0 1.0 CE1 C:HIS77 2.6 31.5 1.0 O C:HOH581 2.7 32.7 1.0 CD2 C:HIS77 3.2 28.1 1.0 ND1 C:HIS77 3.8 25.2 1.0 CG C:HIS77 4.1 26.4 1.0 OD2 C:ASP76 4.5 52.6 1.0 CB C:ASP76 5.0 39.6 1.0

T.Huyton, D.Goerlich. Using the Copper Binding Peptide Ghk For Phasing Structures To Be Published.