Atomistry » Copper » PDB 6pvy-6rhx » 6qpz
Atomistry »
  Copper »
    PDB 6pvy-6rhx »
      6qpz »

Copper in PDB 6qpz: Crystal Structure of As Isolated Y323E Mutant of Haem-Cu Containing Nitrite Reductase From Ralstonia Pickettii

Enzymatic activity of Crystal Structure of As Isolated Y323E Mutant of Haem-Cu Containing Nitrite Reductase From Ralstonia Pickettii

All present enzymatic activity of Crystal Structure of As Isolated Y323E Mutant of Haem-Cu Containing Nitrite Reductase From Ralstonia Pickettii:
1.7.2.1;

Protein crystallography data

The structure of Crystal Structure of As Isolated Y323E Mutant of Haem-Cu Containing Nitrite Reductase From Ralstonia Pickettii, PDB code: 6qpz was solved by S.V.Antonyuk, R.T.Shenoy, T.M.Hedison, R.R.Eady, S.S.Hasnain, N.S.Scrutton, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 42.73 / 1.65
Space group H 3
Cell size a, b, c (Å), α, β, γ (°) 127.550, 127.550, 172.680, 90.00, 90.00, 120.00
R / Rfree (%) 15.8 / 17.8

Other elements in 6qpz:

The structure of Crystal Structure of As Isolated Y323E Mutant of Haem-Cu Containing Nitrite Reductase From Ralstonia Pickettii also contains other interesting chemical elements:

Iron (Fe) 2 atoms

Copper Binding Sites:

The binding sites of Copper atom in the Crystal Structure of As Isolated Y323E Mutant of Haem-Cu Containing Nitrite Reductase From Ralstonia Pickettii (pdb code 6qpz). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 4 binding sites of Copper where determined in the Crystal Structure of As Isolated Y323E Mutant of Haem-Cu Containing Nitrite Reductase From Ralstonia Pickettii, PDB code: 6qpz:
Jump to Copper binding site number: 1; 2; 3; 4;

Copper binding site 1 out of 4 in 6qpz

Go back to Copper Binding Sites List in 6qpz
Copper binding site 1 out of 4 in the Crystal Structure of As Isolated Y323E Mutant of Haem-Cu Containing Nitrite Reductase From Ralstonia Pickettii


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of Crystal Structure of As Isolated Y323E Mutant of Haem-Cu Containing Nitrite Reductase From Ralstonia Pickettii within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cu501

b:18.9
occ:1.00
ND1 A:HIS143 2.0 19.5 1.0
ND1 A:HIS94 2.1 18.3 1.0
SG A:CYS135 2.2 19.0 1.0
SD A:MET148 2.6 18.5 1.0
CE1 A:HIS143 3.0 19.5 1.0
CE1 A:HIS94 3.0 18.4 1.0
CG A:HIS143 3.1 19.2 1.0
CB A:CYS135 3.1 18.3 1.0
CG A:HIS94 3.1 19.1 1.0
CE A:MET148 3.4 18.4 1.0
CB A:HIS143 3.5 18.8 1.0
CB A:HIS94 3.5 19.0 1.0
CA A:HIS94 3.7 19.1 1.0
O A:PRO93 4.1 17.8 1.0
NE2 A:HIS143 4.1 19.7 1.0
CD2 A:HIS143 4.1 19.5 1.0
CG A:MET148 4.2 18.0 1.0
NE2 A:HIS94 4.2 19.2 1.0
CD2 A:HIS94 4.2 19.1 1.0
CB A:THR137 4.3 20.3 1.0
OG1 A:THR137 4.3 19.9 1.0
CE3 A:TRP61 4.5 19.7 1.0
CA A:CYS135 4.5 18.2 1.0
N A:ASN95 4.6 19.1 1.0
CA A:HIS143 4.6 18.5 1.0
CB A:MET148 4.7 17.7 1.0
C A:HIS94 4.7 19.5 1.0
N A:HIS94 4.7 19.2 1.0
C A:PRO93 4.8 19.1 1.0
CZ3 A:TRP61 5.0 19.4 1.0

Copper binding site 2 out of 4 in 6qpz

Go back to Copper Binding Sites List in 6qpz
Copper binding site 2 out of 4 in the Crystal Structure of As Isolated Y323E Mutant of Haem-Cu Containing Nitrite Reductase From Ralstonia Pickettii


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of Crystal Structure of As Isolated Y323E Mutant of Haem-Cu Containing Nitrite Reductase From Ralstonia Pickettii within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cu502

b:20.4
occ:1.00
O A:HOH626 2.0 18.1 0.8
NE2 A:HIS134 2.0 18.5 1.0
NE2 A:HIS99 2.0 18.8 1.0
O A:HOH626 2.2 17.5 0.2
CD2 A:HIS134 2.9 18.6 1.0
CE1 A:HIS99 3.0 18.5 1.0
CD2 A:HIS99 3.1 18.5 1.0
CE1 A:HIS134 3.1 18.1 1.0
OD2 A:ASP97 3.8 25.5 1.0
CG A:HIS134 4.1 18.0 1.0
ND1 A:HIS99 4.1 18.6 1.0
ND1 A:HIS134 4.2 18.6 1.0
CG A:HIS99 4.2 18.2 1.0
CG A:ASP97 4.3 24.0 1.0
OD1 A:ASP97 4.6 26.6 1.0
O A:HOH773 4.7 51.2 1.0
O A:HOH807 5.0 19.9 0.5

Copper binding site 3 out of 4 in 6qpz

Go back to Copper Binding Sites List in 6qpz
Copper binding site 3 out of 4 in the Crystal Structure of As Isolated Y323E Mutant of Haem-Cu Containing Nitrite Reductase From Ralstonia Pickettii


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 3 of Crystal Structure of As Isolated Y323E Mutant of Haem-Cu Containing Nitrite Reductase From Ralstonia Pickettii within 5.0Å range:
probe atom residue distance (Å) B Occ
I:Cu501

b:12.9
occ:1.00
ND1 I:HIS94 2.0 12.0 1.0
ND1 I:HIS143 2.1 11.5 1.0
SG I:CYS135 2.2 13.4 1.0
SD I:MET148 2.6 12.4 1.0
CE1 I:HIS94 2.9 12.1 1.0
CE1 I:HIS143 3.0 11.2 1.0
CG I:HIS143 3.1 11.2 1.0
CG I:HIS94 3.1 12.4 1.0
CB I:CYS135 3.2 12.2 1.0
CE I:MET148 3.5 12.5 1.0
CB I:HIS143 3.5 11.4 1.0
CB I:HIS94 3.5 12.3 1.0
CA I:HIS94 3.7 12.1 1.0
O I:PRO93 4.0 12.2 1.0
NE2 I:HIS94 4.1 12.5 1.0
NE2 I:HIS143 4.1 11.4 1.0
CD2 I:HIS143 4.1 11.3 1.0
CG I:MET148 4.2 11.6 1.0
CD2 I:HIS94 4.2 12.4 1.0
CB I:THR137 4.3 13.6 1.0
OG1 I:THR137 4.3 14.0 1.0
CE3 I:TRP61 4.5 12.7 1.0
CA I:HIS143 4.6 11.4 1.0
N I:ASN95 4.6 11.7 1.0
CA I:CYS135 4.6 12.0 1.0
CB I:MET148 4.7 11.3 1.0
C I:HIS94 4.7 12.2 1.0
N I:HIS94 4.8 12.5 1.0
C I:PRO93 4.8 12.6 1.0

Copper binding site 4 out of 4 in 6qpz

Go back to Copper Binding Sites List in 6qpz
Copper binding site 4 out of 4 in the Crystal Structure of As Isolated Y323E Mutant of Haem-Cu Containing Nitrite Reductase From Ralstonia Pickettii


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 4 of Crystal Structure of As Isolated Y323E Mutant of Haem-Cu Containing Nitrite Reductase From Ralstonia Pickettii within 5.0Å range:
probe atom residue distance (Å) B Occ
I:Cu502

b:13.7
occ:1.00
O I:HOH675 1.7 8.6 0.5
NE2 I:HIS99 2.0 12.6 1.0
NE2 I:HIS134 2.0 12.0 1.0
O I:HOH675 2.2 14.5 0.5
CD2 I:HIS134 2.9 11.8 1.0
CE1 I:HIS99 3.0 12.4 1.0
CD2 I:HIS99 3.1 12.4 1.0
CE1 I:HIS134 3.1 12.0 1.0
OD2 I:ASP97 3.8 20.0 1.0
CG I:HIS134 4.1 11.6 1.0
ND1 I:HIS99 4.1 12.3 1.0
ND1 I:HIS134 4.2 11.9 1.0
CG I:HIS99 4.2 12.3 1.0
CG I:ASP97 4.4 17.5 1.0
OD1 I:ASP97 4.6 20.2 1.0
O I:HOH718 4.6 37.9 1.0

Reference:

T.M.Hedison, R.T.Shenoy, A.I.Iorgu, D.J.Heyes, K.Fisher, G.Wright, S.Hay, R.R.Eady, S.S.Hasnain, N.S.Scrutton. Unexpected Roles of A Tether Harboring A Tyrosine Gatekeeper Residue in Modular Nitrite Reductase Catalysis Acs Catalysis 2019.
ISSN: ESSN 2155-5435
DOI: 10.1021/ACSCATAL.9B01266
Page generated: Sun Dec 13 11:25:00 2020

Last articles

Zn in 8WB0
Zn in 8WAX
Zn in 8WAU
Zn in 8WAZ
Zn in 8WAY
Zn in 8WAV
Zn in 8WAW
Zn in 8WAT
Zn in 8W7M
Zn in 8WD3
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy