Copper in PDB 6p16: Cu-Bound Pcuac Domain From PMOF1

The structure of Cu-Bound Pcuac Domain From PMOF1, PDB code: 6p16 was solved by M.R.Sendzik, O.S.Fisher, A.C.Rosenzweig, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	33.54 / 1.91
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	54.285, 65.818, 77.952, 90.00, 90.00, 90.00
R / Rfree (%)	19.5 / 23.7

The binding sites of Copper atom in the Cu-Bound Pcuac Domain From PMOF1 (pdb code 6p16). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 2 binding sites of Copper where determined in the Cu-Bound Pcuac Domain From PMOF1, PDB code: 6p16:
Jump to Copper binding site number: 1; 2;

Copper binding site 1 out of 2 in the Cu-Bound Pcuac Domain From PMOF1


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of Cu-Bound Pcuac Domain From PMOF1 within 5.0Å range: probe atom residue distance (Å) B Occ B:Cu201 b:39.7 occ:1.00 ND1 B:HIS33 2.0 40.9 1.0 N B:HIS33 2.1 40.3 1.0 ND1 B:HIS44 2.1 47.8 1.0 OE2 A:GLU43 2.3 95.1 1.0 CE1 B:HIS33 2.9 36.1 1.0 O B:HOH350 3.0 54.1 1.0 CG B:HIS44 3.0 44.7 1.0 CG B:HIS33 3.1 38.0 1.0 O B:HIS33 3.1 38.3 1.0 CA B:HIS33 3.1 41.1 1.0 CE1 B:HIS44 3.1 46.2 1.0 CB B:HIS44 3.3 35.0 1.0 CA B:HIS44 3.3 35.1 1.0 CD A:GLU43 3.3 83.2 1.0 CB B:HIS33 3.5 34.3 1.0 C B:HIS33 3.5 40.5 1.0 OE1 A:GLU43 3.9 85.3 1.0 NE2 B:HIS33 4.0 39.9 1.0 N B:HIS44 4.1 30.4 1.0 CD2 B:HIS33 4.1 36.5 1.0 CD2 B:HIS44 4.2 44.0 1.0 NE2 B:HIS44 4.2 43.8 1.0 CG A:GLU43 4.5 54.5 1.0 C B:HIS44 4.5 26.1 1.0 N B:GLU34 4.8 39.5 1.0 O A:HOH362 4.8 49.9 1.0

Copper binding site 2 out of 2 in the Cu-Bound Pcuac Domain From PMOF1


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of Cu-Bound Pcuac Domain From PMOF1 within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu201 b:35.5 occ:1.00 ND1 A:HIS33 2.0 40.5 1.0 N A:HIS33 2.1 37.2 1.0 ND1 A:HIS44 2.1 45.3 1.0 OE2 B:GLU43 2.2 94.7 1.0 O A:HOH352 2.6 45.3 1.0 CE1 A:HIS33 2.9 41.9 1.0 CG A:HIS44 3.1 37.9 1.0 CG A:HIS33 3.1 36.0 1.0 O A:HIS33 3.1 33.9 1.0 CA A:HIS33 3.1 38.4 1.0 CE1 A:HIS44 3.1 36.0 1.0 CD B:GLU43 3.3 79.1 1.0 CB A:HIS44 3.3 30.6 1.0 CA A:HIS44 3.4 26.8 1.0 CB A:HIS33 3.5 32.0 1.0 C A:HIS33 3.5 36.6 1.0 OE1 B:GLU43 3.9 76.4 1.0 N A:HIS44 4.1 31.1 1.0 NE2 A:HIS33 4.1 43.0 1.0 CD2 A:HIS33 4.2 41.3 1.0 CD2 A:HIS44 4.2 38.6 1.0 NE2 A:HIS44 4.2 42.1 1.0 CG B:GLU43 4.4 57.4 1.0 C A:HIS44 4.5 29.0 1.0 N A:GLU34 4.8 32.2 1.0 O B:HOH354 5.0 48.6 1.0

O.S.Fisher, M.R.Sendzik, M.O.Ross, T.J.Lawton, B.M.Hoffman, A.C.Rosenzweig. Pcuac Domains From Methane-Oxidizing Bacteria Use A Histidine Brace to Bind Copper. J.Biol.Chem. V. 294 16351 2019.
ISSN: ESSN 1083-351X
PubMed: 31527086
DOI: 10.1074/JBC.RA119.010093