Copper in PDB 6mf0: Crystal Structure Determination of Human/Porcine Chimera Coagulation Factor VIII

The structure of Crystal Structure Determination of Human/Porcine Chimera Coagulation Factor VIII, PDB code: 6mf0 was solved by I.W.Smith, P.C.Spiegel, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	49.03 / 3.20
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	72.005, 135.863, 196.107, 90.00, 90.15, 90.00
R / Rfree (%)	20.6 / 28.7

The structure of Crystal Structure Determination of Human/Porcine Chimera Coagulation Factor VIII also contains other interesting chemical elements:

Calcium	(Ca)	2 atoms
Zinc	(Zn)	2 atoms

The binding sites of Copper atom in the Crystal Structure Determination of Human/Porcine Chimera Coagulation Factor VIII (pdb code 6mf0). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 2 binding sites of Copper where determined in the Crystal Structure Determination of Human/Porcine Chimera Coagulation Factor VIII, PDB code: 6mf0:
Jump to Copper binding site number: 1; 2;

Copper binding site 1 out of 2 in the Crystal Structure Determination of Human/Porcine Chimera Coagulation Factor VIII


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of Crystal Structure Determination of Human/Porcine Chimera Coagulation Factor VIII within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu2416 b:40.5 occ:1.00 ND1 A:HIS1954 2.2 39.1 1.0 ND1 A:HIS2005 2.3 40.2 1.0 SG A:CYS2000 2.5 37.8 1.0 CG A:HIS1954 2.9 42.8 1.0 CB A:HIS1954 2.9 35.7 1.0 CG A:HIS2005 3.1 37.3 1.0 CA A:HIS1954 3.2 38.7 1.0 CE1 A:HIS2005 3.3 47.5 1.0 CB A:HIS2005 3.3 35.8 1.0 CE1 A:HIS1954 3.3 44.0 1.0 CB A:CYS2000 3.4 32.3 1.0 SD A:MET2010 3.4 39.9 1.0 CD2 A:HIS1954 4.1 43.7 1.0 N A:HIS1954 4.2 35.7 1.0 CB A:ILE2002 4.2 40.0 1.0 CD2 A:HIS2005 4.2 46.7 1.0 N A:SER1955 4.3 31.7 1.0 NE2 A:HIS2005 4.3 50.7 1.0 NE2 A:HIS1954 4.3 45.3 1.0 C A:HIS1954 4.3 38.0 1.0 CD1 A:ILE2002 4.3 40.1 1.0 CG1 A:ILE2002 4.4 39.0 1.0 CG A:MET2010 4.7 34.4 1.0 CA A:CYS2000 4.8 33.5 1.0 CE A:MET2010 4.8 37.4 1.0 CA A:HIS2005 4.8 38.6 1.0 C A:ILE1953 4.8 35.9 1.0 CG2 A:ILE2002 4.8 40.9 1.0 O A:ILE1953 5.0 41.0 1.0

Copper binding site 2 out of 2 in the Crystal Structure Determination of Human/Porcine Chimera Coagulation Factor VIII


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of Crystal Structure Determination of Human/Porcine Chimera Coagulation Factor VIII within 5.0Å range: probe atom residue distance (Å) B Occ B:Cu2421 b:48.4 occ:1.00 ND1 B:HIS1954 2.2 41.5 1.0 ND1 B:HIS2005 2.2 33.4 1.0 SG B:CYS2000 2.5 47.6 1.0 CG B:HIS2005 3.0 37.7 1.0 CE1 B:HIS1954 3.0 43.6 1.0 CB B:HIS2005 3.2 42.4 1.0 CE1 B:HIS2005 3.2 39.8 1.0 CG B:HIS1954 3.3 46.2 1.0 CB B:CYS2000 3.4 42.4 1.0 SD B:MET2010 3.5 43.2 1.0 O B:ILE1953 3.7 52.4 1.0 CB B:HIS1954 3.7 39.5 1.0 CA B:HIS1954 3.9 40.4 1.0 CB B:ILE2002 4.1 41.9 1.0 CD2 B:HIS2005 4.1 41.0 1.0 NE2 B:HIS1954 4.2 43.4 1.0 NE2 B:HIS2005 4.2 45.4 1.0 CD2 B:HIS1954 4.3 46.2 1.0 CG1 B:ILE2002 4.4 38.7 1.0 CD1 B:ILE2002 4.4 43.8 1.0 C B:ILE1953 4.5 49.1 1.0 CG2 B:ILE2002 4.6 42.7 1.0 N B:HIS1954 4.6 43.6 1.0 CE B:MET2010 4.7 46.4 1.0 CA B:HIS2005 4.7 41.2 1.0 CG B:MET2010 4.8 45.1 1.0 CA B:CYS2000 4.8 40.3 1.0 N B:SER1955 4.9 51.8 1.0 C B:HIS1954 5.0 46.0 1.0

I.W.Smith, A.E.D'aquino, C.W.Coyle, A.Fedanov, E.T.Parker, G.Denning, H.T.Spencer, P.Lollar, C.B.Doering, P.C.Spiegel Jr.. The 3.2 Angstrom Structure of A Bioengineered Variant of Blood Coagulation Factor VIII Indicates Two Conformations of the C2 Domain. J.Thromb.Haemost. 2019.
ISSN: ESSN 1538-7836
PubMed: 31454152
DOI: 10.1111/JTH.14621