Copper in PDB 6l58: Cu(II) Loaded Tegillarca Granosa M-Ferritin Soaked with Fe(II)
Enzymatic activity of Cu(II) Loaded Tegillarca Granosa M-Ferritin Soaked with Fe(II)
All present enzymatic activity of Cu(II) Loaded Tegillarca Granosa M-Ferritin Soaked with Fe(II):
1.16.3.1;
Protein crystallography data
The structure of Cu(II) Loaded Tegillarca Granosa M-Ferritin Soaked with Fe(II), PDB code: 6l58
was solved by
Q.Q.Jiang,
X.R.Su,
T.H.Ming,
H.S.Huan,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
36.40 /
3.90
|
Space group
|
H 3 2
|
Cell size a, b, c (Å), α, β, γ (°)
|
217.288,
217.288,
134.083,
90.00,
90.00,
120.00
|
R / Rfree (%)
|
18.7 /
26.4
|
Copper Binding Sites:
The binding sites of Copper atom in the Cu(II) Loaded Tegillarca Granosa M-Ferritin Soaked with Fe(II)
(pdb code 6l58). This binding sites where shown within
5.0 Angstroms radius around Copper atom.
In total 8 binding sites of Copper where determined in the
Cu(II) Loaded Tegillarca Granosa M-Ferritin Soaked with Fe(II), PDB code: 6l58:
Jump to Copper binding site number:
1;
2;
3;
4;
5;
6;
7;
8;
Copper binding site 1 out
of 8 in 6l58
Go back to
Copper Binding Sites List in 6l58
Copper binding site 1 out
of 8 in the Cu(II) Loaded Tegillarca Granosa M-Ferritin Soaked with Fe(II)
Mono view
Stereo pair view
|
A full contact list of Copper with other atoms in the Cu binding
site number 1 of Cu(II) Loaded Tegillarca Granosa M-Ferritin Soaked with Fe(II) within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Cu201
b:0.7
occ:1.00
|
OE2
|
A:GLU25
|
2.8
|
85.3
|
1.0
|
ND1
|
A:HIS63
|
2.9
|
99.0
|
1.0
|
NE2
|
A:GLN139
|
2.9
|
89.8
|
1.0
|
OE1
|
A:GLU60
|
2.9
|
0.0
|
1.0
|
OE2
|
A:GLU60
|
3.0
|
94.3
|
1.0
|
CD
|
A:GLU60
|
3.3
|
0.4
|
1.0
|
CG
|
A:HIS63
|
3.5
|
92.2
|
1.0
|
CE1
|
A:HIS63
|
3.6
|
99.0
|
1.0
|
CD
|
A:GLU25
|
3.7
|
82.8
|
1.0
|
CB
|
A:HIS63
|
3.7
|
84.0
|
1.0
|
OE1
|
A:GLU25
|
3.8
|
80.2
|
1.0
|
CD
|
A:GLN139
|
4.2
|
79.8
|
1.0
|
CD1
|
A:TYR135
|
4.3
|
72.8
|
1.0
|
CG1
|
A:VAL108
|
4.4
|
72.3
|
1.0
|
CD2
|
A:HIS63
|
4.4
|
88.8
|
1.0
|
NE2
|
A:HIS63
|
4.5
|
93.3
|
1.0
|
CE1
|
A:TYR135
|
4.6
|
75.7
|
1.0
|
OE1
|
A:GLU105
|
4.6
|
97.9
|
1.0
|
CG
|
A:GLU60
|
4.7
|
88.8
|
1.0
|
CG
|
A:GLN139
|
4.8
|
78.4
|
1.0
|
|
Copper binding site 2 out
of 8 in 6l58
Go back to
Copper Binding Sites List in 6l58
Copper binding site 2 out
of 8 in the Cu(II) Loaded Tegillarca Granosa M-Ferritin Soaked with Fe(II)
Mono view
Stereo pair view
|
A full contact list of Copper with other atoms in the Cu binding
site number 2 of Cu(II) Loaded Tegillarca Granosa M-Ferritin Soaked with Fe(II) within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Cu202
b:0.2
occ:1.00
|
OE1
|
A:GLU132
|
3.4
|
75.6
|
1.0
|
OE1
|
C:GLU132
|
3.6
|
78.4
|
1.0
|
CE
|
C:MET128
|
3.6
|
86.5
|
1.0
|
CU
|
B:CU201
|
3.9
|
100.0
|
1.0
|
OE2
|
A:GLU132
|
4.1
|
77.2
|
1.0
|
OE2
|
B:GLU132
|
4.1
|
88.8
|
1.0
|
CD
|
A:GLU132
|
4.2
|
78.1
|
1.0
|
OE1
|
B:GLU132
|
4.2
|
75.2
|
1.0
|
OE2
|
C:GLU132
|
4.3
|
82.4
|
1.0
|
OD1
|
C:ASP129
|
4.3
|
95.5
|
1.0
|
CD
|
C:GLU132
|
4.3
|
79.4
|
1.0
|
CD
|
B:GLU132
|
4.6
|
79.0
|
1.0
|
CE
|
A:MET128
|
4.7
|
75.4
|
1.0
|
CE
|
B:MET128
|
4.7
|
78.1
|
1.0
|
|
Copper binding site 3 out
of 8 in 6l58
Go back to
Copper Binding Sites List in 6l58
Copper binding site 3 out
of 8 in the Cu(II) Loaded Tegillarca Granosa M-Ferritin Soaked with Fe(II)
Mono view
Stereo pair view
|
A full contact list of Copper with other atoms in the Cu binding
site number 3 of Cu(II) Loaded Tegillarca Granosa M-Ferritin Soaked with Fe(II) within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Cu201
b:100.0
occ:1.00
|
OE1
|
C:GLU132
|
2.4
|
78.4
|
1.0
|
OD1
|
B:ASP129
|
2.5
|
88.2
|
1.0
|
OD1
|
C:ASP129
|
2.6
|
95.5
|
1.0
|
OE2
|
B:GLU132
|
2.9
|
88.8
|
1.0
|
OE1
|
A:GLU132
|
3.0
|
75.6
|
1.0
|
OD1
|
A:ASP129
|
3.1
|
94.5
|
1.0
|
CD
|
B:GLU132
|
3.1
|
79.0
|
1.0
|
CG
|
C:ASP129
|
3.3
|
90.2
|
1.0
|
OE1
|
B:GLU132
|
3.4
|
75.2
|
1.0
|
OD2
|
C:ASP129
|
3.5
|
85.7
|
1.0
|
CD
|
C:GLU132
|
3.6
|
79.4
|
1.0
|
CG
|
B:ASP129
|
3.7
|
84.8
|
1.0
|
CU
|
A:CU202
|
3.9
|
0.2
|
1.0
|
CG
|
B:GLU132
|
4.0
|
80.9
|
1.0
|
CG
|
A:ASP129
|
4.1
|
93.6
|
1.0
|
CD
|
A:GLU132
|
4.1
|
78.1
|
1.0
|
CB
|
A:ASP129
|
4.3
|
77.7
|
1.0
|
CG
|
C:GLU132
|
4.3
|
76.1
|
1.0
|
OE2
|
C:GLU132
|
4.5
|
82.4
|
1.0
|
CA
|
A:ASP129
|
4.5
|
76.6
|
1.0
|
OD2
|
B:ASP129
|
4.5
|
95.7
|
1.0
|
CB
|
B:ASP129
|
4.5
|
72.7
|
1.0
|
CB
|
C:ASP129
|
4.6
|
80.4
|
1.0
|
CA
|
B:ASP129
|
4.6
|
77.1
|
1.0
|
CA
|
C:ASP129
|
4.8
|
81.5
|
1.0
|
OE2
|
A:GLU132
|
4.8
|
77.2
|
1.0
|
CG
|
A:GLU132
|
4.9
|
82.7
|
1.0
|
|
Copper binding site 4 out
of 8 in 6l58
Go back to
Copper Binding Sites List in 6l58
Copper binding site 4 out
of 8 in the Cu(II) Loaded Tegillarca Granosa M-Ferritin Soaked with Fe(II)
Mono view
Stereo pair view
|
A full contact list of Copper with other atoms in the Cu binding
site number 4 of Cu(II) Loaded Tegillarca Granosa M-Ferritin Soaked with Fe(II) within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Cu202
b:0.8
occ:1.00
|
OE2
|
B:GLU60
|
2.1
|
0.8
|
1.0
|
CD
|
B:GLU60
|
2.9
|
0.2
|
1.0
|
OE1
|
B:GLU60
|
3.0
|
0.6
|
1.0
|
OE2
|
B:GLU25
|
3.0
|
93.4
|
1.0
|
CD2
|
B:HIS63
|
3.1
|
78.9
|
1.0
|
NE2
|
B:GLN139
|
3.3
|
83.4
|
1.0
|
OE1
|
B:GLU25
|
3.3
|
88.3
|
1.0
|
CD
|
B:GLU25
|
3.5
|
93.2
|
1.0
|
OE1
|
B:GLN139
|
4.0
|
94.6
|
1.0
|
CG
|
B:HIS63
|
4.0
|
76.9
|
1.0
|
NE2
|
B:HIS63
|
4.0
|
89.9
|
1.0
|
CD
|
B:GLN139
|
4.1
|
82.1
|
1.0
|
OE1
|
B:GLU105
|
4.2
|
95.1
|
1.0
|
CG1
|
B:VAL108
|
4.3
|
79.4
|
1.0
|
CB
|
B:HIS63
|
4.3
|
74.1
|
1.0
|
CG
|
B:GLU60
|
4.4
|
91.1
|
1.0
|
CB
|
B:GLU60
|
4.9
|
78.8
|
1.0
|
CD1
|
B:TYR135
|
5.0
|
79.3
|
1.0
|
|
Copper binding site 5 out
of 8 in 6l58
Go back to
Copper Binding Sites List in 6l58
Copper binding site 5 out
of 8 in the Cu(II) Loaded Tegillarca Granosa M-Ferritin Soaked with Fe(II)
Mono view
Stereo pair view
|
A full contact list of Copper with other atoms in the Cu binding
site number 5 of Cu(II) Loaded Tegillarca Granosa M-Ferritin Soaked with Fe(II) within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
C:Cu201
b:0.4
occ:1.00
|
OE1
|
C:GLU60
|
2.4
|
0.1
|
1.0
|
OE2
|
C:GLU60
|
2.7
|
93.6
|
1.0
|
CD
|
C:GLU60
|
2.8
|
1.0
|
1.0
|
OE2
|
C:GLU25
|
2.9
|
88.5
|
1.0
|
ND1
|
C:HIS63
|
3.1
|
77.8
|
1.0
|
NE2
|
C:GLN139
|
3.4
|
83.6
|
1.0
|
OE1
|
C:GLU25
|
3.5
|
78.3
|
1.0
|
CD
|
C:GLU25
|
3.6
|
84.8
|
1.0
|
CE1
|
C:HIS63
|
3.8
|
85.1
|
1.0
|
CG
|
C:HIS63
|
3.8
|
80.8
|
1.0
|
CB
|
C:HIS63
|
4.1
|
79.3
|
1.0
|
OE1
|
C:GLU105
|
4.2
|
90.6
|
1.0
|
CG
|
C:GLU60
|
4.3
|
88.7
|
1.0
|
CD
|
C:GLN139
|
4.5
|
74.7
|
1.0
|
CA
|
C:GLU60
|
4.6
|
77.2
|
1.0
|
NE2
|
C:HIS63
|
4.7
|
85.7
|
1.0
|
OE2
|
C:GLU105
|
4.7
|
0.1
|
1.0
|
CD2
|
C:HIS63
|
4.7
|
84.9
|
1.0
|
CB
|
C:GLU60
|
4.7
|
78.9
|
1.0
|
CG1
|
C:VAL108
|
4.9
|
71.5
|
1.0
|
CD
|
C:GLU105
|
4.9
|
97.1
|
1.0
|
|
Copper binding site 6 out
of 8 in 6l58
Go back to
Copper Binding Sites List in 6l58
Copper binding site 6 out
of 8 in the Cu(II) Loaded Tegillarca Granosa M-Ferritin Soaked with Fe(II)
Mono view
Stereo pair view
|
A full contact list of Copper with other atoms in the Cu binding
site number 6 of Cu(II) Loaded Tegillarca Granosa M-Ferritin Soaked with Fe(II) within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
D:Cu201
b:0.3
occ:1.00
|
OE2
|
D:GLU60
|
2.1
|
0.8
|
1.0
|
OE2
|
D:GLU25
|
2.7
|
83.8
|
1.0
|
CD
|
D:GLU60
|
2.8
|
99.2
|
1.0
|
CD2
|
D:HIS63
|
2.8
|
90.1
|
1.0
|
OE1
|
D:GLU60
|
2.8
|
92.5
|
1.0
|
NE2
|
D:GLN139
|
2.9
|
0.3
|
1.0
|
OE1
|
D:GLU25
|
3.1
|
93.1
|
1.0
|
CD
|
D:GLU25
|
3.3
|
86.3
|
1.0
|
NE2
|
D:HIS63
|
3.6
|
99.1
|
1.0
|
CG
|
D:HIS63
|
3.7
|
85.2
|
1.0
|
OE1
|
D:GLU105
|
4.0
|
91.2
|
1.0
|
CG1
|
D:VAL108
|
4.0
|
69.5
|
1.0
|
CD
|
D:GLN139
|
4.1
|
89.3
|
1.0
|
CG
|
D:GLU60
|
4.3
|
91.0
|
1.0
|
CB
|
D:HIS63
|
4.3
|
88.1
|
1.0
|
CE1
|
D:HIS63
|
4.6
|
94.8
|
1.0
|
ND1
|
D:HIS63
|
4.7
|
79.5
|
1.0
|
CB
|
D:GLU60
|
4.8
|
80.3
|
1.0
|
CG
|
D:GLU25
|
4.8
|
75.7
|
1.0
|
OE1
|
D:GLN139
|
4.9
|
87.4
|
1.0
|
CD
|
D:GLU105
|
4.9
|
95.5
|
1.0
|
CA
|
D:GLU60
|
4.9
|
76.0
|
1.0
|
OE2
|
D:GLU105
|
5.0
|
87.6
|
1.0
|
|
Copper binding site 7 out
of 8 in 6l58
Go back to
Copper Binding Sites List in 6l58
Copper binding site 7 out
of 8 in the Cu(II) Loaded Tegillarca Granosa M-Ferritin Soaked with Fe(II)
Mono view
Stereo pair view
|
A full contact list of Copper with other atoms in the Cu binding
site number 7 of Cu(II) Loaded Tegillarca Granosa M-Ferritin Soaked with Fe(II) within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
D:Cu202
b:89.4
occ:0.34
|
CU
|
D:CU203
|
3.6
|
94.7
|
0.3
|
OE2
|
D:GLU132
|
3.9
|
80.0
|
1.0
|
OE1
|
D:GLU132
|
4.3
|
74.7
|
1.0
|
CD
|
D:GLU132
|
4.5
|
79.5
|
1.0
|
SD
|
D:MET128
|
4.5
|
100.0
|
1.0
|
|
Copper binding site 8 out
of 8 in 6l58
Go back to
Copper Binding Sites List in 6l58
Copper binding site 8 out
of 8 in the Cu(II) Loaded Tegillarca Granosa M-Ferritin Soaked with Fe(II)
Mono view
Stereo pair view
|
A full contact list of Copper with other atoms in the Cu binding
site number 8 of Cu(II) Loaded Tegillarca Granosa M-Ferritin Soaked with Fe(II) within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
D:Cu203
b:94.7
occ:0.33
|
OE2
|
D:GLU132
|
2.3
|
80.0
|
1.0
|
OD1
|
D:ASP129
|
2.7
|
93.6
|
1.0
|
CD
|
D:GLU132
|
3.5
|
79.5
|
1.0
|
CU
|
D:CU202
|
3.6
|
89.4
|
0.3
|
CG
|
D:ASP129
|
3.7
|
92.5
|
1.0
|
CG
|
D:GLU132
|
4.3
|
86.8
|
1.0
|
OE1
|
D:GLU132
|
4.3
|
74.7
|
1.0
|
OD2
|
D:ASP129
|
4.5
|
94.8
|
1.0
|
CB
|
D:ASP129
|
4.6
|
83.4
|
1.0
|
O
|
D:HOH301
|
4.6
|
0.9
|
0.3
|
CA
|
D:ASP129
|
4.7
|
82.8
|
1.0
|
|
Reference:
Q.Q.Jiang,
X.R.Su,
T.H.Ming,
H.S.Huan.
Crystal Structure Analysis Unravels How CU2+ in CU2+-Ferritin Nanocage Perturb the Functions of Tegillarca Granosa Ferritin To Be Published.
Page generated: Wed Jul 31 06:38:04 2024
|