Copper in PDB 6ek9: Cytosolic Copper Storage Protein Csp From Streptomyces Lividans: Cu Loaded Form

The structure of Cytosolic Copper Storage Protein Csp From Streptomyces Lividans: Cu Loaded Form, PDB code: 6ek9 was solved by M.L.Straw, A.K.Chaplin, M.A.Hough, J.A.R.Worrall, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	45.22 / 1.50
Space group	I 2 2 2
Cell size a, b, c (Å), α, β, γ (°)	62.081, 64.138, 66.003, 90.00, 90.00, 90.00
R / Rfree (%)	20.6 / 23.1

Pages:

>>> Page 1 <<< Page 2, Binding sites: 11 - 20;

Binding sites:

The binding sites of Copper atom in the Cytosolic Copper Storage Protein Csp From Streptomyces Lividans: Cu Loaded Form (pdb code 6ek9). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 20 binding sites of Copper where determined in the Cytosolic Copper Storage Protein Csp From Streptomyces Lividans: Cu Loaded Form, PDB code: 6ek9:
Jump to Copper binding site number: 1; 2; 3; 4; 5; 6; 7; 8; 9; 10;

Copper binding site 1 out of 20 in the Cytosolic Copper Storage Protein Csp From Streptomyces Lividans: Cu Loaded Form


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of Cytosolic Copper Storage Protein Csp From Streptomyces Lividans: Cu Loaded Form within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu200 b:23.3 occ:0.90 SG A:CYS100 2.2 25.3 1.0 SG A:CYS38 2.2 25.5 1.0 OD1 A:ASP61 2.4 27.3 1.0 CU A:CU1201 2.9 22.3 0.9 CU A:CU1207 3.0 28.8 0.9 CU A:CU1218 3.0 29.7 0.9 SG A:CYS121 3.2 26.9 1.0 CU A:CU1206 3.2 26.3 0.9 CB A:CYS100 3.2 25.9 1.0 CB A:CYS38 3.3 20.4 1.0 CG A:ASP61 3.5 25.7 1.0 CA A:ASP61 3.5 21.6 1.0 CB A:CYS64 3.5 19.1 1.0 CU A:CU1202 3.8 20.1 0.9 SG A:CYS64 4.0 20.6 1.0 SG A:CYS117 4.0 29.5 1.0 CB A:ASP61 4.1 23.6 1.0 CA A:CYS38 4.2 21.4 1.0 N A:ASP61 4.2 23.1 1.0 CA A:CYS100 4.5 29.5 1.0 SG A:CYS104 4.5 33.6 1.0 O A:ASP61 4.5 18.0 1.0 C A:ASP61 4.5 19.9 1.0 O A:THR60 4.5 21.4 1.0 OD2 A:ASP61 4.5 28.5 1.0 O A:CYS100 4.6 32.2 1.0 C A:THR60 4.6 22.2 1.0 C A:CYS100 4.7 30.9 1.0 CA A:CYS64 4.9 17.4 1.0 N A:CYS38 5.0 23.0 1.0 CB A:CYS121 5.0 28.1 1.0

Copper binding site 2 out of 20 in the Cytosolic Copper Storage Protein Csp From Streptomyces Lividans: Cu Loaded Form


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of Cytosolic Copper Storage Protein Csp From Streptomyces Lividans: Cu Loaded Form within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu201 b:22.3 occ:0.90 SG A:CYS121 2.2 26.9 1.0 SG A:CYS64 2.2 20.6 1.0 CU A:CU1202 2.8 20.1 0.9 CU A:CU1215 2.9 22.1 0.9 CU A:CU1200 2.9 23.3 0.9 SG A:CYS38 3.0 25.5 1.0 CB A:CYS64 3.2 19.1 1.0 CB A:CYS121 3.4 28.1 1.0 SG A:CYS34 3.4 23.8 1.0 CB A:CYS100 3.5 25.9 1.0 CU A:CU1216 3.5 17.8 0.9 CU A:CU1206 3.6 26.3 0.9 SG A:CYS100 3.9 25.3 1.0 CA A:CYS121 4.0 28.9 1.0 SG A:CYS97 4.1 26.0 1.0 CA A:CYS97 4.1 25.1 1.0 CB A:CYS97 4.5 26.3 1.0 CA A:CYS64 4.5 17.4 1.0 CB A:CYS38 4.6 20.4 1.0 O A:CYS97 4.6 30.0 1.0 N A:CYS121 4.7 30.2 1.0 CA A:CYS100 4.7 29.5 1.0 O A:VAL96 4.8 24.0 1.0 CU A:CU1218 4.9 29.7 0.9 C A:CYS97 4.9 27.8 1.0 N A:CYS97 4.9 23.7 1.0 C A:CYS100 4.9 30.9 1.0 C A:CYS64 4.9 16.1 1.0 N A:GLY101 5.0 32.5 1.0

Copper binding site 3 out of 20 in the Cytosolic Copper Storage Protein Csp From Streptomyces Lividans: Cu Loaded Form


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 3 of Cytosolic Copper Storage Protein Csp From Streptomyces Lividans: Cu Loaded Form within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu202 b:20.1 occ:0.90 SG A:CYS38 2.0 25.5 1.0 SG A:CYS34 2.1 23.8 1.0 O A:CYS34 2.3 19.6 1.0 CU A:CU1201 2.8 22.3 0.9 C A:CYS34 2.9 18.9 1.0 CB A:CYS34 3.2 23.4 1.0 CB A:CYS38 3.2 20.4 1.0 CU A:CU1215 3.3 22.1 0.9 CA A:CYS34 3.4 20.7 1.0 CU A:CU1206 3.7 26.3 0.9 N A:CYS38 3.8 23.0 1.0 CU A:CU1216 3.8 17.8 0.9 CU A:CU1200 3.8 23.3 0.9 SG A:CYS121 3.9 26.9 1.0 N A:ALA35 4.0 17.8 1.0 SG A:CYS64 4.1 20.6 1.0 CA A:CYS38 4.1 21.4 1.0 CA A:ALA35 4.5 16.6 1.0 SG A:CYS117 4.6 29.5 1.0 CB A:CYS64 4.6 19.1 1.0 CB A:ALA37 4.7 26.3 1.0 SG A:CYS68 4.7 17.9 1.0 N A:CYS34 4.8 20.7 1.0 N A:CYS121 4.8 30.2 1.0 C A:ALA37 4.8 25.4 1.0 CA A:CYS121 4.9 28.9 1.0 CB A:CYS121 4.9 28.1 1.0 C A:ALA35 4.9 16.8 1.0

Copper binding site 4 out of 20 in the Cytosolic Copper Storage Protein Csp From Streptomyces Lividans: Cu Loaded Form


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 4 of Cytosolic Copper Storage Protein Csp From Streptomyces Lividans: Cu Loaded Form within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu203 b:51.9 occ:0.90 ND1 A:HIS111 2.1 56.6 1.0 SG A:CYS114 2.2 44.6 1.0 CE1 A:HIS111 3.1 61.1 1.0 CG A:HIS111 3.1 58.9 1.0 CB A:CYS114 3.1 45.8 1.0 CE1 A:HIS113 3.2 46.5 1.0 CU A:CU1219 3.3 46.5 0.9 CU A:CU1204 3.4 42.6 0.9 CB A:HIS107 3.4 55.4 1.0 CB A:HIS111 3.4 56.4 1.0 SG A:CYS45 3.5 44.1 1.0 ND1 A:HIS113 3.5 45.2 1.0 CU A:CU1217 4.0 40.4 0.9 CG A:HIS107 4.0 54.7 1.0 ND1 A:HIS107 4.0 52.1 1.0 NE2 A:HIS111 4.2 63.7 1.0 CD2 A:HIS111 4.2 62.6 1.0 O A:HIS107 4.3 60.0 1.0 CA A:CYS114 4.3 44.4 1.0 NE2 A:HIS113 4.3 48.1 1.0 N A:CYS114 4.3 44.5 1.0 CU A:CU1205 4.4 36.1 0.9 CA A:HIS111 4.4 60.2 1.0 N A:HIS111 4.5 62.7 1.0 O A:HIS111 4.5 55.1 1.0 CA A:HIS107 4.7 57.8 1.0 CG A:HIS113 4.7 44.1 1.0 CD1 A:LEU54 4.8 54.5 1.0 C A:HIS107 4.8 58.5 1.0 CD2 A:LEU54 4.8 55.1 1.0 C A:HIS111 4.9 57.5 1.0

Copper binding site 5 out of 20 in the Cytosolic Copper Storage Protein Csp From Streptomyces Lividans: Cu Loaded Form


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 5 of Cytosolic Copper Storage Protein Csp From Streptomyces Lividans: Cu Loaded Form within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu204 b:42.6 occ:0.90 ND1 A:HIS113 1.9 45.2 1.0 SG A:CYS41 2.2 34.9 1.0 SG A:CYS114 2.4 44.6 1.0 CU A:CU1205 2.4 36.1 0.9 CE1 A:HIS113 2.9 46.5 1.0 CG A:HIS113 2.9 44.1 1.0 N A:CYS114 3.0 44.5 1.0 C A:HIS113 3.2 42.1 1.0 CA A:CYS114 3.3 44.4 1.0 CB A:HIS113 3.3 43.7 1.0 CU A:CU1203 3.4 51.9 0.9 CB A:CYS114 3.4 45.8 1.0 SG A:CYS45 3.4 44.1 1.0 CB A:CYS41 3.6 31.2 1.0 O A:HIS113 3.7 40.1 1.0 CA A:HIS113 3.8 43.9 1.0 CA A:CYS41 4.0 30.7 1.0 NE2 A:HIS113 4.0 48.1 1.0 O A:CYS41 4.0 33.9 1.0 CD2 A:HIS113 4.0 47.9 1.0 CU A:CU1217 4.1 40.4 0.9 CU A:CU1208 4.2 36.1 0.9 C A:CYS41 4.4 32.9 1.0 CU A:CU1219 4.5 46.5 0.9 N A:HIS113 4.6 47.8 1.0 CB A:ALA44 4.7 39.1 1.0 C A:CYS114 4.8 44.3 1.0 CB A:HIS111 4.9 56.4 1.0 N A:CYS45 5.0 40.1 1.0 ND1 A:HIS111 5.0 56.6 1.0 CB A:CYS45 5.0 41.4 1.0

Copper binding site 6 out of 20 in the Cytosolic Copper Storage Protein Csp From Streptomyces Lividans: Cu Loaded Form


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 6 of Cytosolic Copper Storage Protein Csp From Streptomyces Lividans: Cu Loaded Form within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu205 b:36.1 occ:0.90 SG A:CYS45 2.1 44.1 1.0 SG A:CYS41 2.1 34.9 1.0 O A:CYS41 2.3 33.9 1.0 CU A:CU1204 2.4 42.6 0.9 CU A:CU1217 2.7 40.4 0.9 C A:CYS41 2.8 32.9 1.0 CB A:CYS41 3.2 31.2 1.0 CB A:CYS45 3.2 41.4 1.0 CA A:CYS41 3.3 30.7 1.0 CU A:CU1208 3.3 36.1 0.9 SG A:CYS114 3.5 44.6 1.0 ND1 A:HIS113 3.6 45.2 1.0 N A:CYS45 3.7 40.1 1.0 N A:ALA42 3.7 28.6 1.0 OD2 A:ASP61 3.9 28.5 1.0 CE1 A:HIS113 4.0 46.5 1.0 CA A:CYS45 4.0 41.9 1.0 SG A:CYS57 4.1 45.1 1.0 CA A:ALA42 4.3 28.4 1.0 CU A:CU1203 4.4 51.9 0.9 CU A:CU1219 4.5 46.5 0.9 CG A:HIS113 4.6 44.1 1.0 CB A:ALA44 4.7 39.1 1.0 C A:ALA42 4.7 30.0 1.0 C A:ALA44 4.7 41.6 1.0 N A:CYS41 4.8 27.7 1.0 O A:ALA42 4.8 32.1 1.0

Copper binding site 7 out of 20 in the Cytosolic Copper Storage Protein Csp From Streptomyces Lividans: Cu Loaded Form


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 7 of Cytosolic Copper Storage Protein Csp From Streptomyces Lividans: Cu Loaded Form within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu206 b:26.3 occ:0.90 SG A:CYS121 2.3 26.9 1.0 SG A:CYS117 2.3 29.5 1.0 O A:CYS117 2.4 34.4 1.0 CU A:CU1207 2.8 28.8 0.9 C A:CYS117 2.9 34.7 1.0 CU A:CU1200 3.2 23.3 0.9 SG A:CYS38 3.3 25.5 1.0 CB A:CYS117 3.4 34.1 1.0 CB A:CYS121 3.5 28.1 1.0 CA A:CYS117 3.5 34.7 1.0 CU A:CU1201 3.6 22.3 0.9 CU A:CU1202 3.7 20.1 0.9 N A:CYS121 3.8 30.2 1.0 N A:ALA118 3.9 37.3 1.0 CU A:CU1218 3.9 29.7 0.9 OD1 A:ASP61 4.1 27.3 1.0 CA A:CYS121 4.2 28.9 1.0 CA A:ALA118 4.3 39.4 1.0 SG A:CYS104 4.4 33.6 1.0 SG A:CYS34 4.5 23.8 1.0 SG A:CYS100 4.6 25.3 1.0 CB A:ALA120 4.7 31.1 1.0 O A:CYS100 4.7 32.2 1.0 CB A:CYS38 4.8 20.4 1.0 C A:ALA120 4.8 30.3 1.0 C A:ALA118 4.9 39.4 1.0 N A:CYS38 4.9 23.0 1.0 CA A:CYS38 4.9 21.4 1.0 N A:CYS117 4.9 38.3 1.0 CB A:CYS104 4.9 37.6 1.0 O A:ALA118 5.0 41.3 1.0 CB A:CYS100 5.0 25.9 1.0

Copper binding site 8 out of 20 in the Cytosolic Copper Storage Protein Csp From Streptomyces Lividans: Cu Loaded Form


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 8 of Cytosolic Copper Storage Protein Csp From Streptomyces Lividans: Cu Loaded Form within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu207 b:28.8 occ:0.90 OD1 A:ASP61 2.1 27.3 1.0 SG A:CYS117 2.3 29.5 1.0 SG A:CYS104 2.3 33.6 1.0 CU A:CU1218 2.7 29.7 0.9 CU A:CU1206 2.8 26.3 0.9 CU A:CU1208 2.8 36.1 0.9 CU A:CU1200 3.0 23.3 0.9 CG A:ASP61 3.1 25.7 1.0 CB A:CYS117 3.2 34.1 1.0 OD2 A:ASP61 3.4 28.5 1.0 CB A:CYS104 3.6 37.6 1.0 SG A:CYS100 3.9 25.3 1.0 SG A:CYS38 3.9 25.5 1.0 CB A:CYS41 3.9 31.2 1.0 SG A:CYS41 4.0 34.9 1.0 SG A:CYS121 4.2 26.9 1.0 C A:CYS117 4.3 34.7 1.0 CA A:CYS117 4.3 34.7 1.0 O A:CYS100 4.4 32.2 1.0 CA A:CYS38 4.5 21.4 1.0 CB A:ASP61 4.5 23.6 1.0 O A:CYS117 4.5 34.4 1.0 CB A:CYS38 4.7 20.4 1.0 N A:ALA118 4.8 37.3 1.0 CA A:CYS104 4.9 40.4 1.0 CA A:ASP61 5.0 21.6 1.0

Copper binding site 9 out of 20 in the Cytosolic Copper Storage Protein Csp From Streptomyces Lividans: Cu Loaded Form


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 9 of Cytosolic Copper Storage Protein Csp From Streptomyces Lividans: Cu Loaded Form within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu208 b:36.1 occ:0.90 OD2 A:ASP61 2.1 28.5 1.0 SG A:CYS104 2.3 33.6 1.0 SG A:CYS41 2.4 34.9 1.0 SG A:CYS57 2.6 45.1 1.0 CU A:CU1207 2.8 28.8 0.9 CG A:ASP61 2.9 25.7 1.0 OD1 A:ASP61 3.1 27.3 1.0 CU A:CU1217 3.3 40.4 0.9 CU A:CU1205 3.3 36.1 0.9 CB A:CYS41 3.4 31.2 1.0 CB A:CYS104 3.7 37.6 1.0 SG A:CYS114 3.9 44.6 1.0 CU A:CU1219 3.9 46.5 0.9 CU A:CU1218 3.9 29.7 0.9 O A:CYS57 4.0 30.4 1.0 CU A:CU1204 4.2 42.6 0.9 CB A:CYS57 4.2 38.3 1.0 CB A:ASP61 4.3 23.6 1.0 CA A:CYS104 4.4 40.4 1.0 SG A:CYS117 4.5 29.5 1.0 C A:CYS57 4.5 32.7 1.0 C A:CYS41 4.5 32.9 1.0 CA A:CYS41 4.6 30.7 1.0 SG A:CYS45 4.7 44.1 1.0 CB A:CYS117 4.7 34.1 1.0 N A:ALA42 4.7 28.6 1.0 CA A:CYS57 4.8 36.2 1.0 O A:CYS41 4.9 33.9 1.0

Copper binding site 10 out of 20 in the Cytosolic Copper Storage Protein Csp From Streptomyces Lividans: Cu Loaded Form


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 10 of Cytosolic Copper Storage Protein Csp From Streptomyces Lividans: Cu Loaded Form within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu209 b:20.6 occ:0.90 SG A:CYS68 2.1 17.9 1.0 SG A:CYS124 2.2 23.1 1.0 CU A:CU1214 2.7 19.6 0.9 CU A:CU1213 3.0 20.5 0.9 CU A:CU1215 3.1 22.1 0.9 SG A:CYS97 3.2 26.0 1.0 CB A:CYS124 3.4 25.7 1.0 CU A:CU1212 3.4 23.9 0.9 CB A:CYS68 3.5 14.9 1.0 SG A:CYS93 3.5 18.7 1.0 CA A:CYS31 3.6 18.7 1.0 CU A:CU1216 3.7 17.8 0.9 CB A:CYS34 3.7 23.4 1.0 SG A:CYS31 3.7 19.3 1.0 CB A:CYS31 4.1 18.2 1.0 SG A:CYS34 4.1 23.8 1.0 O A:GLU30 4.2 22.3 1.0 CA A:CYS68 4.3 14.1 1.0 N A:CYS31 4.3 21.2 1.0 O A:CYS31 4.3 17.5 1.0 C A:CYS31 4.5 17.8 1.0 C A:GLU30 4.5 23.4 1.0 CA A:CYS124 4.6 29.5 1.0 C A:CYS124 4.9 29.1 1.0 CA A:CYS34 5.0 20.7 1.0 CB A:CYS97 5.0 26.3 1.0

M.L.Straw, A.K.Chaplin, M.A.Hough, J.Paps, V.N.Bavro, M.T.Wilson, E.Vijgenboom, J.A.R.Worrall. A Cytosolic Copper Storage Protein Provides A Second Level of Copper Tolerance in Streptomyces Lividans. Metallomics V. 10 180 2018.
ISSN: ESSN 1756-591X
PubMed: 29292456
DOI: 10.1039/C7MT00299H