Copper in PDB 5zlm: Mutation in the Trinuclear Site of Cota-Laccase: H491C Mutant, pH 8.0

The structure of Mutation in the Trinuclear Site of Cota-Laccase: H491C Mutant, pH 8.0, PDB code: 5zlm was solved by T.Xie, Z.C.Liu, G.G.Wang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	24.40 / 1.70
Space group	P 31 2 1
Cell size a, b, c (Å), α, β, γ (°)	101.709, 101.709, 135.769, 90.00, 90.00, 120.00
R / Rfree (%)	16.1 / 18.2

The binding sites of Copper atom in the Mutation in the Trinuclear Site of Cota-Laccase: H491C Mutant, pH 8.0 (pdb code 5zlm). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 3 binding sites of Copper where determined in the Mutation in the Trinuclear Site of Cota-Laccase: H491C Mutant, pH 8.0, PDB code: 5zlm:
Jump to Copper binding site number: 1; 2; 3;

Copper binding site 1 out of 3 in the Mutation in the Trinuclear Site of Cota-Laccase: H491C Mutant, pH 8.0


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of Mutation in the Trinuclear Site of Cota-Laccase: H491C Mutant, pH 8.0 within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu601 b:11.7 occ:0.90 ND1 A:HIS419 2.1 12.2 1.0 ND1 A:HIS497 2.1 12.1 1.0 SG A:CYS492 2.2 11.0 1.0 CE1 A:HIS419 2.9 13.2 1.0 CG A:HIS497 3.1 11.4 1.0 SD A:MET502 3.1 12.8 1.0 CE1 A:HIS497 3.1 12.6 1.0 CG A:HIS419 3.1 11.7 1.0 CB A:CYS492 3.2 10.2 1.0 CB A:HIS497 3.4 11.0 1.0 CB A:HIS419 3.6 11.3 1.0 CE A:MET502 3.7 15.6 1.0 CD1 A:ILE494 4.0 9.9 1.0 NE2 A:HIS419 4.1 13.3 1.0 CA A:HIS419 4.1 11.5 1.0 CB A:ILE494 4.2 8.8 1.0 CD2 A:HIS419 4.2 12.5 1.0 CD2 A:HIS497 4.2 11.6 1.0 NE2 A:HIS497 4.2 11.7 1.0 CG1 A:ILE494 4.4 8.6 1.0 CG A:MET502 4.5 12.0 1.0 CA A:CYS492 4.6 10.7 1.0 CD A:PRO420 4.7 9.1 1.0 O A:HOH968 4.8 18.4 1.0 CA A:HIS497 4.9 10.7 1.0 CD1 A:LEU386 4.9 27.6 1.0 N A:ILE494 4.9 9.2 1.0

Copper binding site 2 out of 3 in the Mutation in the Trinuclear Site of Cota-Laccase: H491C Mutant, pH 8.0


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of Mutation in the Trinuclear Site of Cota-Laccase: H491C Mutant, pH 8.0 within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu602 b:9.4 occ:0.17 NE2 A:HIS422 1.7 9.2 1.0 NE2 A:HIS105 1.7 8.6 1.0 CD2 A:HIS422 2.6 8.8 1.0 CE1 A:HIS105 2.6 8.2 1.0 CE1 A:HIS422 2.7 8.3 1.0 O A:HOH932 2.8 10.3 1.0 CD2 A:HIS105 2.8 8.5 1.0 NE2 A:HIS424 3.4 9.2 1.0 CD2 A:HIS424 3.5 9.0 1.0 O A:HOH1168 3.5 25.6 1.0 ND1 A:HIS107 3.6 9.3 1.0 CE1 A:HIS424 3.6 8.6 1.0 CG A:HIS107 3.7 7.9 1.0 CG A:HIS424 3.7 8.7 1.0 ND1 A:HIS105 3.8 8.4 1.0 CA A:HIS107 3.8 6.9 1.0 CG A:HIS422 3.8 8.4 1.0 ND1 A:HIS422 3.8 8.5 1.0 ND1 A:HIS424 3.8 8.6 1.0 CG A:HIS105 3.9 7.7 1.0 CE1 A:HIS107 3.9 8.8 1.0 CD2 A:HIS107 4.0 8.8 1.0 N A:GLY108 4.1 7.6 1.0 CB A:HIS107 4.1 7.4 1.0 NE2 A:HIS107 4.1 9.3 1.0 CU A:CU603 4.2 8.3 0.5 O A:HOH707 4.3 28.1 1.0 C A:HIS107 4.5 7.2 1.0 CA A:HIS424 4.5 8.8 1.0 CB A:HIS424 4.6 8.9 1.0 O A:HOH860 4.6 9.4 1.0 N A:HIS107 4.7 6.5 1.0 O A:LEU106 4.8 6.7 1.0 N A:HIS424 4.9 8.9 1.0 O A:LEU423 4.9 9.0 1.0 O A:HOH989 4.9 10.0 1.0

Copper binding site 3 out of 3 in the Mutation in the Trinuclear Site of Cota-Laccase: H491C Mutant, pH 8.0


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 3 of Mutation in the Trinuclear Site of Cota-Laccase: H491C Mutant, pH 8.0 within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu603 b:8.3 occ:0.46 ND1 A:HIS107 1.8 9.3 1.0 NE2 A:HIS153 2.0 8.0 1.0 NE2 A:HIS493 2.1 16.4 1.0 CE1 A:HIS107 2.6 8.8 1.0 O A:HOH1168 2.6 25.6 1.0 CE1 A:HIS153 2.9 8.1 1.0 CG A:HIS107 2.9 7.9 1.0 CD2 A:HIS493 3.1 15.5 1.0 CE1 A:HIS493 3.1 14.3 1.0 CD2 A:HIS153 3.1 7.9 1.0 CB A:HIS107 3.5 7.4 1.0 O A:HOH781 3.6 29.0 1.0 CD2 A:HIS105 3.7 8.5 1.0 NE2 A:HIS107 3.7 9.3 1.0 CD2 A:HIS107 3.9 8.8 1.0 ND1 A:HIS153 4.1 7.9 1.0 ND1 A:HIS493 4.1 13.5 1.0 CZ2 A:TRP151 4.1 6.7 1.0 CG A:HIS493 4.1 12.8 1.0 CU A:CU602 4.2 9.4 0.2 CG A:HIS153 4.2 6.9 1.0 NE2 A:HIS105 4.3 8.6 1.0 CD2 A:HIS422 4.4 8.8 1.0 CE2 A:TRP151 4.4 6.6 1.0 CB A:ALA297 4.5 7.1 1.0 NE2 A:HIS422 4.5 9.2 1.0 NE1 A:TRP151 4.6 6.5 1.0 CA A:HIS107 4.6 6.9 1.0 O A:HOH707 4.6 28.1 1.0 CH2 A:TRP151 4.8 7.4 1.0 CG A:HIS105 4.9 7.7 1.0

T.Xie, Z.Liu, G.Wang. Structural Insight Into the Allosteric Coupling of CU1 Site and Trinuclear Cu Cluster in Cota Laccase Chembiochem 2018.
ISSN: ESSN 1439-7633
PubMed: 29722464
DOI: 10.1002/CBIC.201800236