Copper in PDB 5zll: Mutation in the Trinuclear Site of Cota-Laccase: H493C Mutant, pH 8.0

The structure of Mutation in the Trinuclear Site of Cota-Laccase: H493C Mutant, pH 8.0, PDB code: 5zll was solved by T.Xie, Z.C.Liu, G.G.Wang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.90 / 2.60
Space group	P 31 2 1
Cell size a, b, c (Å), α, β, γ (°)	101.827, 101.827, 135.439, 90.00, 90.00, 120.00
R / Rfree (%)	17 / 21.5

The binding sites of Copper atom in the Mutation in the Trinuclear Site of Cota-Laccase: H493C Mutant, pH 8.0 (pdb code 5zll). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 3 binding sites of Copper where determined in the Mutation in the Trinuclear Site of Cota-Laccase: H493C Mutant, pH 8.0, PDB code: 5zll:
Jump to Copper binding site number: 1; 2; 3;

Copper binding site 1 out of 3 in the Mutation in the Trinuclear Site of Cota-Laccase: H493C Mutant, pH 8.0


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of Mutation in the Trinuclear Site of Cota-Laccase: H493C Mutant, pH 8.0 within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu601 b:27.9 occ:0.85 ND1 A:HIS419 2.0 31.3 1.0 ND1 A:HIS497 2.0 27.2 1.0 SG A:CYS492 2.3 31.1 1.0 CE1 A:HIS419 2.8 30.7 1.0 CE1 A:HIS497 3.0 26.9 1.0 CG A:HIS497 3.0 26.1 1.0 CG A:HIS419 3.1 31.1 1.0 SD A:MET502 3.2 23.5 1.0 CB A:CYS492 3.3 26.2 1.0 CB A:HIS497 3.4 26.6 1.0 CB A:HIS419 3.6 29.3 1.0 CE A:MET502 3.7 25.6 1.0 CD1 A:ILE494 4.0 25.0 1.0 NE2 A:HIS419 4.0 28.7 1.0 NE2 A:HIS497 4.1 26.5 1.0 CB A:ILE494 4.1 26.3 1.0 CD2 A:HIS497 4.2 25.6 1.0 CD2 A:HIS419 4.2 30.8 1.0 CA A:HIS419 4.3 27.4 1.0 CG1 A:ILE494 4.5 25.3 1.0 CA A:CYS492 4.7 26.7 1.0 CD A:PRO420 4.7 26.4 1.0 CG A:MET502 4.7 24.8 1.0 CG2 A:ILE494 4.8 25.6 1.0 O A:HOH712 4.9 30.1 1.0 N A:ILE494 4.9 25.7 1.0 CA A:HIS497 4.9 27.3 1.0 O A:THR418 4.9 23.6 1.0 O A:ILE494 5.0 29.1 1.0

Copper binding site 2 out of 3 in the Mutation in the Trinuclear Site of Cota-Laccase: H493C Mutant, pH 8.0


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of Mutation in the Trinuclear Site of Cota-Laccase: H493C Mutant, pH 8.0 within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu602 b:34.6 occ:0.29 NE2 A:HIS424 1.8 21.4 1.0 NE2 A:HIS491 1.9 33.1 1.0 NE2 A:HIS155 2.5 30.5 1.0 CE1 A:HIS424 2.6 22.4 1.0 CD2 A:HIS491 2.7 31.5 1.0 CD2 A:HIS424 2.9 22.6 1.0 CE1 A:HIS491 3.0 32.4 1.0 CD2 A:HIS155 3.3 30.8 1.0 CD2 A:HIS422 3.4 23.9 1.0 CE1 A:HIS155 3.6 31.2 1.0 O A:HOH838 3.7 42.9 1.0 CU A:CU603 3.8 27.8 0.3 ND1 A:HIS424 3.8 23.1 1.0 CD2 A:HIS105 3.9 19.9 1.0 CG A:HIS491 3.9 28.8 1.0 CG A:HIS424 3.9 22.9 1.0 NE2 A:HIS422 3.9 25.1 1.0 NE2 A:HIS105 3.9 20.0 1.0 CG2 A:VAL489 3.9 27.4 1.0 ND1 A:HIS491 4.0 31.7 1.0 CG A:HIS155 4.5 26.9 1.0 CG A:HIS105 4.6 19.7 1.0 CG A:HIS422 4.6 24.4 1.0 ND1 A:HIS155 4.6 31.1 1.0 CE1 A:HIS105 4.7 20.7 1.0 O A:HOH836 4.8 41.1 1.0 OE2 A:GLU498 5.0 38.4 1.0 CE1 A:HIS107 5.0 21.0 1.0

Copper binding site 3 out of 3 in the Mutation in the Trinuclear Site of Cota-Laccase: H493C Mutant, pH 8.0


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 3 of Mutation in the Trinuclear Site of Cota-Laccase: H493C Mutant, pH 8.0 within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu603 b:27.8 occ:0.26 NE2 A:HIS422 1.8 25.1 1.0 NE2 A:HIS105 1.9 20.0 1.0 CE1 A:HIS422 2.7 25.5 1.0 CD2 A:HIS422 2.8 23.9 1.0 CD2 A:HIS105 2.8 19.9 1.0 O A:HOH752 2.9 20.8 1.0 CE1 A:HIS105 3.0 20.7 1.0 CG A:HIS107 3.2 19.6 1.0 ND1 A:HIS107 3.4 20.2 1.0 CD2 A:HIS107 3.4 19.3 1.0 CA A:HIS107 3.4 19.1 1.0 NE2 A:HIS424 3.6 21.4 1.0 CD2 A:HIS424 3.6 22.6 1.0 CE1 A:HIS107 3.7 21.0 1.0 NE2 A:HIS107 3.7 20.2 1.0 CB A:HIS107 3.8 18.6 1.0 CU A:CU602 3.8 34.6 0.3 ND1 A:HIS422 3.8 25.8 1.0 N A:GLY108 3.8 19.3 1.0 CG A:HIS422 3.8 24.4 1.0 CE1 A:HIS424 3.9 22.4 1.0 CG A:HIS105 4.0 19.7 1.0 CG A:HIS424 4.0 22.9 1.0 ND1 A:HIS105 4.0 21.1 1.0 ND1 A:HIS424 4.1 23.1 1.0 C A:HIS107 4.1 19.5 1.0 N A:HIS107 4.5 18.4 1.0 CA A:HIS424 4.6 22.6 1.0 O A:LEU106 4.6 19.0 1.0 CB A:HIS424 4.8 23.1 1.0 O A:HOH798 4.9 19.8 1.0 C A:LEU106 4.9 19.3 1.0 O A:HOH750 4.9 17.3 1.0

T.Xie, Z.Liu, G.Wang. Structural Insight Into the Allosteric Coupling of CU1 Site and Trinuclear Cu Cluster in Cota Laccase Chembiochem 2018.
ISSN: ESSN 1439-7633
PubMed: 29722464
DOI: 10.1002/CBIC.201800236