Copper in PDB 5zlk: Mutation in the Trinuclear Site of Cota-Laccase: H493A Mutant, pH 8.0

The structure of Mutation in the Trinuclear Site of Cota-Laccase: H493A Mutant, pH 8.0, PDB code: 5zlk was solved by T.Xie, Z.C.Liu, G.G.Wang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.00 / 2.60
Space group	P 31 2 1
Cell size a, b, c (Å), α, β, γ (°)	102.123, 102.123, 135.691, 90.00, 90.00, 120.00
R / Rfree (%)	17.2 / 22.9

The binding sites of Copper atom in the Mutation in the Trinuclear Site of Cota-Laccase: H493A Mutant, pH 8.0 (pdb code 5zlk). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 4 binding sites of Copper where determined in the Mutation in the Trinuclear Site of Cota-Laccase: H493A Mutant, pH 8.0, PDB code: 5zlk:
Jump to Copper binding site number: 1; 2; 3; 4;

Copper binding site 1 out of 4 in the Mutation in the Trinuclear Site of Cota-Laccase: H493A Mutant, pH 8.0


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of Mutation in the Trinuclear Site of Cota-Laccase: H493A Mutant, pH 8.0 within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu601 b:32.5 occ:1.00 ND1 A:HIS419 2.0 35.8 1.0 ND1 A:HIS497 2.1 34.1 1.0 SG A:CYS492 2.2 33.9 1.0 CE1 A:HIS419 2.7 36.7 1.0 CE1 A:HIS497 3.0 35.7 1.0 CG A:HIS497 3.1 34.4 1.0 CB A:CYS492 3.1 29.7 1.0 CG A:HIS419 3.2 36.1 1.0 SD A:MET502 3.4 33.3 1.0 CB A:HIS497 3.4 33.5 1.0 CE A:MET502 3.7 31.2 1.0 CB A:HIS419 3.7 34.7 1.0 CD1 A:ILE494 3.9 26.4 1.0 NE2 A:HIS419 3.9 34.0 1.0 CB A:ILE494 4.2 27.6 1.0 CD2 A:HIS419 4.2 37.2 1.0 NE2 A:HIS497 4.2 35.2 1.0 CD2 A:HIS497 4.2 33.7 1.0 CA A:HIS419 4.2 33.1 1.0 CG1 A:ILE494 4.5 27.1 1.0 CA A:CYS492 4.5 29.4 1.0 CD A:PRO420 4.6 33.8 1.0 CG A:MET502 4.7 33.4 1.0 O A:HOH762 4.7 37.9 1.0 O A:THR418 4.8 30.8 1.0 CG2 A:ILE494 4.9 27.6 1.0 CA A:HIS497 4.9 35.0 1.0 CD1 A:LEU386 5.0 54.0 1.0

Copper binding site 2 out of 4 in the Mutation in the Trinuclear Site of Cota-Laccase: H493A Mutant, pH 8.0


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of Mutation in the Trinuclear Site of Cota-Laccase: H493A Mutant, pH 8.0 within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu602 b:23.5 occ:0.84 NE2 A:HIS105 1.9 19.3 1.0 NE2 A:HIS422 2.0 26.9 1.0 O A:HOH847 2.5 22.9 1.0 CE1 A:HIS422 2.9 25.6 1.0 CE1 A:HIS105 2.9 20.8 1.0 CD2 A:HIS105 2.9 19.2 1.0 O A:HOH871 2.9 23.3 1.0 CD2 A:HIS422 3.0 24.7 1.0 ND1 A:HIS107 3.2 31.4 1.0 NE2 A:HIS424 3.3 22.7 1.0 CD2 A:HIS424 3.3 24.5 1.0 CG A:HIS107 3.4 28.9 1.0 CE1 A:HIS107 3.5 30.8 1.0 CE1 A:HIS424 3.7 24.1 1.0 CA A:HIS107 3.7 24.9 1.0 CU A:CU603 3.7 26.7 0.8 CG A:HIS424 3.8 25.6 1.0 CD2 A:HIS107 3.9 30.9 1.0 NE2 A:HIS107 3.9 31.3 1.0 ND1 A:HIS424 3.9 25.2 1.0 ND1 A:HIS105 4.0 21.7 1.0 CB A:HIS107 4.0 26.1 1.0 ND1 A:HIS422 4.0 24.6 1.0 CG A:HIS105 4.0 20.5 1.0 N A:GLY108 4.1 24.4 1.0 CG A:HIS422 4.1 23.4 1.0 CU A:CU604 4.3 28.6 0.2 CA A:HIS424 4.4 24.4 1.0 C A:HIS107 4.4 25.0 1.0 CB A:HIS424 4.6 26.7 1.0 N A:HIS107 4.7 23.7 1.0 O A:HOH850 4.7 20.0 1.0 O A:HOH750 4.7 23.8 1.0 O A:LEU106 4.8 24.1 1.0 N A:HIS424 4.9 25.5 1.0 O A:LEU423 4.9 30.4 1.0 NE2 A:HIS491 4.9 33.7 1.0

Copper binding site 3 out of 4 in the Mutation in the Trinuclear Site of Cota-Laccase: H493A Mutant, pH 8.0


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 3 of Mutation in the Trinuclear Site of Cota-Laccase: H493A Mutant, pH 8.0 within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu603 b:26.7 occ:0.76 O A:HOH871 2.0 23.3 1.0 NE2 A:HIS424 2.1 22.7 1.0 NE2 A:HIS155 2.1 26.3 1.0 NE2 A:HIS491 2.1 33.7 1.0 O A:HOH841 2.7 35.9 1.0 CE1 A:HIS424 2.9 24.1 1.0 CD2 A:HIS491 3.0 32.5 1.0 CD2 A:HIS155 3.0 26.6 1.0 CE1 A:HIS155 3.1 28.4 1.0 CD2 A:HIS424 3.1 24.5 1.0 CE1 A:HIS491 3.2 33.6 1.0 CD2 A:HIS422 3.7 24.7 1.0 CU A:CU602 3.7 23.5 0.8 CD2 A:HIS105 3.9 19.2 1.0 NE2 A:HIS105 4.0 19.3 1.0 ND1 A:HIS424 4.1 25.2 1.0 NE2 A:HIS422 4.1 26.9 1.0 CG A:HIS491 4.1 31.7 1.0 CU A:CU604 4.2 28.6 0.2 CG2 A:VAL489 4.2 31.4 1.0 ND1 A:HIS491 4.2 33.1 1.0 ND1 A:HIS155 4.2 27.7 1.0 CG A:HIS155 4.2 24.7 1.0 CG A:HIS424 4.2 25.6 1.0 OE2 A:GLU498 4.5 31.7 1.0 CG A:HIS105 4.6 20.5 1.0 O A:HOH915 4.7 42.9 1.0 O A:HOH825 4.7 34.0 1.0 CE1 A:HIS105 4.8 20.8 1.0 OE1 A:GLU498 4.8 35.9 1.0 ND1 A:HIS107 4.8 31.4 1.0 CD A:GLU498 4.9 33.6 1.0 CG A:HIS422 4.9 23.4 1.0 CE1 A:HIS107 5.0 30.8 1.0

Copper binding site 4 out of 4 in the Mutation in the Trinuclear Site of Cota-Laccase: H493A Mutant, pH 8.0


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 4 of Mutation in the Trinuclear Site of Cota-Laccase: H493A Mutant, pH 8.0 within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu604 b:28.6 occ:0.20 ND1 A:HIS107 2.1 31.4 1.0 NE2 A:HIS153 2.1 26.5 1.0 O A:HOH871 2.3 23.3 1.0 O A:HOH915 2.5 42.9 1.0 CE1 A:HIS107 2.7 30.8 1.0 CE1 A:HIS153 2.8 24.9 1.0 O A:HOH841 2.8 35.9 1.0 CD2 A:HIS153 3.3 26.4 1.0 CG A:HIS107 3.3 28.9 1.0 CB A:HIS107 3.9 26.1 1.0 NE2 A:HIS107 4.0 31.3 1.0 CZ2 A:TRP151 4.0 28.2 1.0 CD2 A:HIS105 4.0 19.2 1.0 ND1 A:HIS153 4.0 23.8 1.0 CU A:CU603 4.2 26.7 0.8 CD2 A:HIS107 4.3 30.9 1.0 CG A:HIS153 4.3 23.1 1.0 CU A:CU602 4.3 23.5 0.8 O A:HOH824 4.4 27.0 1.0 CE2 A:TRP151 4.5 27.2 1.0 CH2 A:TRP151 4.5 28.7 1.0 NE2 A:HIS105 4.6 19.3 1.0 CE1 A:HIS491 4.7 33.6 1.0 CD2 A:HIS422 4.7 24.7 1.0 NE1 A:TRP151 4.8 29.9 1.0 CB A:ALA297 4.8 26.3 1.0 NE2 A:HIS422 4.8 26.9 1.0 NE2 A:HIS491 4.8 33.7 1.0 CD2 A:HIS155 4.8 26.6 1.0 NE2 A:HIS155 4.9 26.3 1.0 CA A:HIS107 5.0 24.9 1.0

T.Xie, Z.Liu, G.Wang. Structural Insight Into the Allosteric Coupling of CU1 Site and Trinuclear Cu Cluster in Cota Laccase Chembiochem 2018.
ISSN: ESSN 1439-7633
PubMed: 29722464
DOI: 10.1002/CBIC.201800236