Atomistry » Copper » PDB 5wbd-5z85 » 5x19
Atomistry »
  Copper »
    PDB 5wbd-5z85 »
      5x19 »

Copper in PDB 5x19: Co Bound Cytochrome C Oxidase at 100 Micro Sec After Pump Laser Irradiation to Release Co From O2 Reduction Center

Enzymatic activity of Co Bound Cytochrome C Oxidase at 100 Micro Sec After Pump Laser Irradiation to Release Co From O2 Reduction Center

All present enzymatic activity of Co Bound Cytochrome C Oxidase at 100 Micro Sec After Pump Laser Irradiation to Release Co From O2 Reduction Center:
1.9.3.1;

Protein crystallography data

The structure of Co Bound Cytochrome C Oxidase at 100 Micro Sec After Pump Laser Irradiation to Release Co From O2 Reduction Center, PDB code: 5x19 was solved by A.Shimada, M.Kubo, S.Baba, K.Yamashita, K.Hirata, G.Ueno, T.Nomura, T.Kimura, K.Shinzawa-Itoh, J.Baba, K.Hatano, Y.Eto, A.Miyamoto, H.Murakami, T.Kumasaka, S.Owada, K.Tono, M.Yabashi, Y.Yamaguchi, S.Yanagisawa, M.Sakaguchi, T.Ogura, R.Komiya, J.Yan, E.Yamashita, M.Yamamoto, H.Ago, S.Yoshikawa, T.Tsukihara, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 25.00 / 2.20
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 185.034, 208.846, 178.046, 90.00, 90.00, 90.00
R / Rfree (%) 17.3 / 21

Other elements in 5x19:

The structure of Co Bound Cytochrome C Oxidase at 100 Micro Sec After Pump Laser Irradiation to Release Co From O2 Reduction Center also contains other interesting chemical elements:

Magnesium (Mg) 2 atoms
Zinc (Zn) 2 atoms
Iron (Fe) 4 atoms
Sodium (Na) 2 atoms

Copper Binding Sites:

The binding sites of Copper atom in the Co Bound Cytochrome C Oxidase at 100 Micro Sec After Pump Laser Irradiation to Release Co From O2 Reduction Center (pdb code 5x19). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 6 binding sites of Copper where determined in the Co Bound Cytochrome C Oxidase at 100 Micro Sec After Pump Laser Irradiation to Release Co From O2 Reduction Center, PDB code: 5x19:
Jump to Copper binding site number: 1; 2; 3; 4; 5; 6;

Copper binding site 1 out of 6 in 5x19

Go back to Copper Binding Sites List in 5x19
Copper binding site 1 out of 6 in the Co Bound Cytochrome C Oxidase at 100 Micro Sec After Pump Laser Irradiation to Release Co From O2 Reduction Center


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of Co Bound Cytochrome C Oxidase at 100 Micro Sec After Pump Laser Irradiation to Release Co From O2 Reduction Center within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cu603

b:45.3
occ:1.00
 ND1 A:HIS240 2.0 41.5 1.0
NE2 A:HIS291 2.0 38.2 1.0
NE2 A:HIS290 2.1 41.5 1.0
O A:CMO606 2.3 35.1 0.2
CD2 A:HIS291 2.8 37.6 1.0
CE1 A:HIS240 2.9 38.6 1.0
CE1 A:HIS290 2.9 41.5 1.0
CG A:HIS240 3.0 42.1 1.0
C A:CMO606 3.0 33.9 0.2
CE1 A:HIS291 3.1 39.7 1.0
CD2 A:HIS290 3.2 42.8 1.0
CB A:HIS240 3.3 41.5 1.0
CA A:HIS240 4.0 41.7 1.0
CG A:HIS291 4.0 40.6 1.0
NE2 A:HIS240 4.0 38.7 1.0
ND1 A:HIS290 4.1 39.8 1.0
CD2 A:HIS240 4.1 36.6 1.0
ND1 A:HIS291 4.1 39.2 1.0
CG A:HIS290 4.2 44.9 1.0
C1A A:HEA602 4.6 41.6 1.0
C4A A:HEA602 4.8 42.3 1.0
NA A:HEA602 4.8 43.7 1.0
CG2 A:VAL243 4.8 39.9 1.0
N A:HIS240 4.9 45.4 1.0
C2A A:HEA602 4.9 41.6 1.0
C3A A:HEA602 5.0 39.5 1.0

Copper binding site 2 out of 6 in 5x19

Go back to Copper Binding Sites List in 5x19
Copper binding site 2 out of 6 in the Co Bound Cytochrome C Oxidase at 100 Micro Sec After Pump Laser Irradiation to Release Co From O2 Reduction Center


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of Co Bound Cytochrome C Oxidase at 100 Micro Sec After Pump Laser Irradiation to Release Co From O2 Reduction Center within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Cu301

b:48.1
occ:1.00
 CU1 B:CUA301 0.0 48.1 1.0
ND1 B:HIS161 2.0 44.4 1.0
SG B:CYS200 2.3 45.7 1.0
SG B:CYS196 2.4 46.3 1.0
SD B:MET207 2.4 51.1 1.0
CU2 B:CUA301 2.6 50.1 1.0
CE1 B:HIS161 2.8 39.2 1.0
CG B:HIS161 3.1 46.7 1.0
CB B:CYS200 3.2 42.6 1.0
CE B:MET207 3.4 44.7 1.0
CB B:CYS196 3.4 42.2 1.0
CG B:MET207 3.5 42.5 1.0
CB B:HIS161 3.6 44.1 1.0
NE2 B:HIS161 4.1 43.7 1.0
CD2 B:HIS161 4.2 40.5 1.0
CA B:HIS161 4.3 42.2 1.0
O B:GLU198 4.3 42.2 1.0
ND1 B:HIS204 4.4 41.8 1.0
CD1 B:TRP104 4.6 53.1 1.0
CA B:CYS200 4.6 49.8 1.0
O B:HIS102 4.7 49.9 1.0
O B:LEU160 4.7 44.1 1.0
CA B:CYS196 4.8 41.3 1.0
N B:CYS200 4.8 51.1 1.0
CA B:HIS204 4.8 44.0 1.0
CB B:MET207 4.9 42.2 1.0

Copper binding site 3 out of 6 in 5x19

Go back to Copper Binding Sites List in 5x19
Copper binding site 3 out of 6 in the Co Bound Cytochrome C Oxidase at 100 Micro Sec After Pump Laser Irradiation to Release Co From O2 Reduction Center


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 3 of Co Bound Cytochrome C Oxidase at 100 Micro Sec After Pump Laser Irradiation to Release Co From O2 Reduction Center within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Cu301

b:50.1
occ:1.00
 CU2 B:CUA301 0.0 50.1 1.0
ND1 B:HIS204 1.9 41.8 1.0
SG B:CYS200 2.3 45.7 1.0
SG B:CYS196 2.3 46.3 1.0
CU1 B:CUA301 2.6 48.1 1.0
CE1 B:HIS204 2.7 46.4 1.0
O B:GLU198 2.8 42.2 1.0
CG B:HIS204 3.1 45.9 1.0
CB B:CYS196 3.2 42.2 1.0
CB B:CYS200 3.4 42.6 1.0
CA B:HIS204 3.6 44.0 1.0
N B:CYS200 3.6 51.1 1.0
CB B:HIS204 3.6 42.4 1.0
C B:GLU198 3.8 43.5 1.0
O B:HIS204 3.8 49.9 1.0
NE2 B:HIS204 3.9 51.3 1.0
CD2 B:HIS204 4.1 42.3 1.0
CA B:CYS200 4.1 49.8 1.0
C B:HIS204 4.1 46.9 1.0
C B:CYS196 4.2 40.7 1.0
ND1 B:HIS161 4.2 44.4 1.0
C B:ILE199 4.2 47.2 1.0
O B:CYS196 4.2 43.2 1.0
CA B:ILE199 4.3 46.1 1.0
CA B:CYS196 4.3 41.3 1.0
N B:GLU198 4.3 41.1 1.0
SD B:MET207 4.4 51.1 1.0
N B:ILE199 4.5 43.0 1.0
N B:SER197 4.6 44.3 1.0
CG B:MET207 4.7 42.5 1.0
CA B:GLU198 4.7 45.5 1.0
N B:HIS204 4.8 48.4 1.0

Copper binding site 4 out of 6 in 5x19

Go back to Copper Binding Sites List in 5x19
Copper binding site 4 out of 6 in the Co Bound Cytochrome C Oxidase at 100 Micro Sec After Pump Laser Irradiation to Release Co From O2 Reduction Center


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 4 of Co Bound Cytochrome C Oxidase at 100 Micro Sec After Pump Laser Irradiation to Release Co From O2 Reduction Center within 5.0Å range:
probe atom residue distance (Å) B Occ
N:Cu603

b:51.3
occ:1.00
 ND1 N:HIS240 1.8 45.1 1.0
NE2 N:HIS291 2.0 46.1 1.0
NE2 N:HIS290 2.1 49.1 1.0
C N:CMO606 2.7 50.4 0.2
CE1 N:HIS240 2.8 40.6 1.0
CG N:HIS240 2.9 49.2 1.0
CE1 N:HIS291 2.9 48.3 1.0
CE1 N:HIS290 2.9 47.8 1.0
CD2 N:HIS291 3.0 42.2 1.0
CD2 N:HIS290 3.1 49.0 1.0
CB N:HIS240 3.3 44.4 1.0
O N:CMO606 3.5 47.0 0.2
NE2 N:HIS240 3.9 47.8 1.0
ND1 N:HIS291 4.0 46.3 1.0
CD2 N:HIS240 4.0 41.5 1.0
CA N:HIS240 4.0 51.4 1.0
CG N:HIS291 4.0 46.6 1.0
ND1 N:HIS290 4.1 45.5 1.0
CG N:HIS290 4.2 45.0 1.0
C1A N:HEA602 4.6 48.8 1.0
NA N:HEA602 4.8 50.1 1.0
C4A N:HEA602 4.8 45.7 1.0
CG2 N:VAL243 4.8 44.6 1.0
C2A N:HEA602 4.9 48.1 1.0
N N:HIS240 4.9 50.3 1.0
C3A N:HEA602 4.9 46.7 1.0

Copper binding site 5 out of 6 in 5x19

Go back to Copper Binding Sites List in 5x19
Copper binding site 5 out of 6 in the Co Bound Cytochrome C Oxidase at 100 Micro Sec After Pump Laser Irradiation to Release Co From O2 Reduction Center


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 5 of Co Bound Cytochrome C Oxidase at 100 Micro Sec After Pump Laser Irradiation to Release Co From O2 Reduction Center within 5.0Å range:
probe atom residue distance (Å) B Occ
O:Cu301

b:59.9
occ:1.00
 CU1 O:CUA301 0.0 59.9 1.0
ND1 O:HIS161 2.1 55.5 1.0
O O:HOH404 2.4 0.7 1.0
SG O:CYS196 2.4 51.3 1.0
SD O:MET207 2.5 56.7 1.0
SG O:CYS200 2.5 52.8 1.0
CU2 O:CUA301 2.6 56.9 1.0
CE1 O:HIS161 3.0 57.2 1.0
CG O:HIS161 3.1 57.8 1.0
CE O:MET207 3.2 47.5 1.0
CB O:CYS200 3.3 43.8 1.0
CB O:CYS196 3.3 53.2 1.0
CB O:HIS161 3.5 50.0 1.0
CG O:MET207 3.5 53.4 1.0
O O:HOH478 3.7 0.1 1.0
NE2 O:HIS161 4.2 55.4 1.0
CD2 O:HIS161 4.2 52.1 1.0
CA O:HIS161 4.3 55.4 1.0
O O:GLU198 4.4 55.6 1.0
ND1 O:HIS204 4.6 56.2 1.0
CD1 O:TRP104 4.6 59.5 1.0
CA O:CYS200 4.7 51.7 1.0
CA O:CYS196 4.8 55.1 1.0
O O:LEU160 4.8 57.2 1.0
N O:CYS200 4.8 57.4 1.0
O O:HIS102 4.8 57.6 1.0
CZ2 O:TRP106 4.9 57.6 1.0
CB O:MET207 4.9 51.1 1.0
CA O:HIS204 5.0 61.4 1.0
O O:HIS204 5.0 54.2 1.0

Copper binding site 6 out of 6 in 5x19

Go back to Copper Binding Sites List in 5x19
Copper binding site 6 out of 6 in the Co Bound Cytochrome C Oxidase at 100 Micro Sec After Pump Laser Irradiation to Release Co From O2 Reduction Center


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 6 of Co Bound Cytochrome C Oxidase at 100 Micro Sec After Pump Laser Irradiation to Release Co From O2 Reduction Center within 5.0Å range:
probe atom residue distance (Å) B Occ
O:Cu301

b:56.9
occ:1.00
 CU2 O:CUA301 0.0 56.9 1.0
ND1 O:HIS204 2.1 56.2 1.0
SG O:CYS196 2.3 51.3 1.0
SG O:CYS200 2.4 52.8 1.0
CU1 O:CUA301 2.6 59.9 1.0
O O:HOH404 2.6 0.7 1.0
O O:GLU198 2.7 55.6 1.0
CE1 O:HIS204 2.9 53.3 1.0
CG O:HIS204 3.2 56.5 1.0
CB O:CYS196 3.2 53.2 1.0
N O:CYS200 3.4 57.4 1.0
CB O:CYS200 3.5 43.8 1.0
CA O:HIS204 3.6 61.4 1.0
CB O:HIS204 3.7 57.0 1.0
C O:GLU198 3.7 46.7 1.0
O O:HIS204 3.8 54.2 1.0
NE2 O:HIS204 4.1 52.9 1.0
CA O:CYS200 4.1 51.7 1.0
C O:HIS204 4.1 56.5 1.0
C O:CYS196 4.2 54.2 1.0
C O:ILE199 4.2 55.4 1.0
CA O:ILE199 4.2 55.2 1.0
O O:CYS196 4.2 55.3 1.0
N O:GLU198 4.2 60.8 1.0
CD2 O:HIS204 4.3 50.3 1.0
ND1 O:HIS161 4.3 55.5 1.0
CA O:CYS196 4.3 55.1 1.0
N O:ILE199 4.4 48.5 1.0
SD O:MET207 4.5 56.7 1.0
N O:SER197 4.5 55.9 1.0
CA O:GLU198 4.7 55.0 1.0
CG O:MET207 4.7 53.4 1.0
N O:HIS204 4.8 50.6 1.0
C O:CYS200 5.0 56.2 1.0

Reference:

A.Shimada, M.Kubo, S.Baba, K.Yamashita, K.Hirata, G.Ueno, T.Nomura, T.Kimura, K.Shinzawa-Itoh, J.Baba, K.Hatano, Y.Eto, A.Miyamoto, H.Murakami, T.Kumasaka, S.Owada, K.Tono, M.Yabashi, Y.Yamaguchi, S.Yanagisawa, M.Sakaguchi, T.Ogura, R.Komiya, J.Yan, E.Yamashita, M.Yamamoto, H.Ago, S.Yoshikawa, T.Tsukihara. A Nanosecond Time-Resolved Xfel Analysis of Structural Changes Associated with Co Release From Cytochrome C Oxidase. Sci Adv V. 3 03042 2017.
ISSN: ESSN 2375-2548
PubMed: 28740863
DOI: 10.1126/SCIADV.1603042
Page generated: Wed Jul 31 05:21:17 2024

Last articles

Zn in 9JPJ
Zn in 9JP7
Zn in 9JPK
Zn in 9JPL
Zn in 9GN6
Zn in 9GN7
Zn in 9GKU
Zn in 9GKW
Zn in 9GKX
Zn in 9GL0
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy