Copper in PDB 5nq7: Crystal Structure of Laccases From Pycnoporus Sanguineus, Izoform I

All present enzymatic activity of Crystal Structure of Laccases From Pycnoporus Sanguineus, Izoform I:
1.10.3.2;

The structure of Crystal Structure of Laccases From Pycnoporus Sanguineus, Izoform I, PDB code: 5nq7 was solved by M.Orlikowska, M.De J.Rostro-Alanis, A.Bujacz, C.Hernandez-Luna, R.Rubio, R.Parra, G.Bujacz, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	46.39 / 2.75
Space group	P 43 3 2
Cell size a, b, c (Å), α, β, γ (°)	185.550, 185.550, 185.550, 90.00, 90.00, 90.00
R / Rfree (%)	16.1 / 20.7

The binding sites of Copper atom in the Crystal Structure of Laccases From Pycnoporus Sanguineus, Izoform I (pdb code 5nq7). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 4 binding sites of Copper where determined in the Crystal Structure of Laccases From Pycnoporus Sanguineus, Izoform I, PDB code: 5nq7:
Jump to Copper binding site number: 1; 2; 3; 4;

Copper binding site 1 out of 4 in the Crystal Structure of Laccases From Pycnoporus Sanguineus, Izoform I


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of Crystal Structure of Laccases From Pycnoporus Sanguineus, Izoform I within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu617 b:47.2 occ:1.00 NE2 A:HIS421 2.1 43.0 1.0 NE2 A:HIS132 2.1 41.2 1.0 O1 A:PER621 2.1 57.2 1.0 O2 A:PER621 2.2 45.9 1.0 NE2 A:HIS471 2.2 48.9 1.0 CE1 A:HIS421 2.9 43.4 1.0 CD2 A:HIS132 3.1 43.7 1.0 CE1 A:HIS132 3.1 42.9 1.0 CE1 A:HIS471 3.1 50.3 1.0 CD2 A:HIS421 3.2 43.1 1.0 CD2 A:HIS471 3.2 50.6 1.0 CD2 A:HIS419 3.9 45.3 1.0 CU A:CU619 4.1 45.9 1.0 ND1 A:HIS421 4.1 43.5 1.0 ND1 A:HIS132 4.2 45.8 1.0 CG A:HIS132 4.2 43.8 1.0 CG A:HIS421 4.2 42.9 1.0 ND1 A:HIS471 4.2 50.5 1.0 CG A:HIS471 4.3 48.8 1.0 CD2 A:PHE469 4.3 39.4 1.0 CD2 A:HIS85 4.4 42.9 1.0 CB A:PHE469 4.5 44.7 1.0 NE2 A:HIS85 4.6 42.5 1.0 CG2 A:VAL100 4.6 46.1 1.0 NE2 A:HIS419 4.7 44.8 1.0 CU A:CU618 4.7 45.0 1.0 NE2 A:HIS473 4.9 46.1 1.0 CG A:PHE469 4.9 40.6 1.0 CE1 A:HIS130 4.9 38.7 1.0 CD2 A:HIS473 5.0 46.6 1.0

Copper binding site 2 out of 4 in the Crystal Structure of Laccases From Pycnoporus Sanguineus, Izoform I


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of Crystal Structure of Laccases From Pycnoporus Sanguineus, Izoform I within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu618 b:45.0 occ:1.00 ND1 A:HIS87 2.1 46.0 1.0 NE2 A:HIS130 2.2 36.7 1.0 NE2 A:HIS473 2.2 46.1 1.0 O2 A:PER621 2.6 45.9 1.0 CD2 A:HIS130 3.1 37.0 1.0 CE1 A:HIS87 3.1 48.7 1.0 O1 A:PER621 3.1 57.2 1.0 CE1 A:HIS473 3.1 45.6 1.0 CG A:HIS87 3.1 46.7 1.0 CE1 A:HIS130 3.2 38.7 1.0 CD2 A:HIS473 3.2 46.6 1.0 CB A:HIS87 3.5 43.0 1.0 CU A:CU619 3.7 45.9 1.0 CD2 A:HIS85 3.8 42.9 1.0 CZ2 A:TRP128 3.8 37.9 1.0 CE2 A:TRP128 4.1 36.7 1.0 NE1 A:TRP128 4.2 37.3 1.0 NE2 A:HIS87 4.2 48.8 1.0 CG A:HIS130 4.2 37.2 1.0 ND1 A:HIS473 4.2 50.0 1.0 CD2 A:HIS87 4.3 49.1 1.0 ND1 A:HIS130 4.3 39.2 1.0 NE2 A:HIS85 4.3 42.5 1.0 CG A:HIS473 4.3 46.3 1.0 CD2 A:HIS419 4.4 45.3 1.0 CB A:ALA262 4.5 44.2 1.0 NE2 A:HIS419 4.5 44.8 1.0 CA A:HIS87 4.5 39.4 1.0 CH2 A:TRP128 4.5 38.2 1.0 CU A:CU617 4.7 47.2 1.0 CG A:HIS85 5.0 45.0 1.0

Copper binding site 3 out of 4 in the Crystal Structure of Laccases From Pycnoporus Sanguineus, Izoform I


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 3 of Crystal Structure of Laccases From Pycnoporus Sanguineus, Izoform I within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu619 b:45.9 occ:1.00 NE2 A:HIS419 2.0 44.8 1.0 NE2 A:HIS85 2.1 42.5 1.0 O A:HOH750 2.2 37.8 1.0 O2 A:PER621 2.7 45.9 1.0 CD2 A:HIS419 2.7 45.3 1.0 CD2 A:HIS85 2.9 42.9 1.0 CE1 A:HIS419 3.1 45.4 1.0 CE1 A:HIS85 3.1 45.3 1.0 ND1 A:HIS87 3.3 46.0 1.0 NE2 A:HIS421 3.3 43.0 1.0 CE1 A:HIS421 3.4 43.4 1.0 CD2 A:HIS421 3.6 43.1 1.0 CG A:HIS87 3.7 46.7 1.0 CU A:CU618 3.7 45.0 1.0 ND1 A:HIS421 3.8 43.5 1.0 CE1 A:HIS87 3.8 48.7 1.0 CA A:HIS87 3.8 39.4 1.0 CG A:HIS421 3.9 42.9 1.0 CG A:HIS419 3.9 45.0 1.0 ND1 A:HIS419 4.0 44.6 1.0 CB A:HIS87 4.1 43.0 1.0 CU A:CU617 4.1 47.2 1.0 CG A:HIS85 4.1 45.0 1.0 N A:GLY88 4.1 39.4 1.0 ND1 A:HIS85 4.1 45.7 1.0 O1 A:PER621 4.2 57.2 1.0 CD2 A:HIS87 4.3 49.1 1.0 NE2 A:HIS87 4.4 48.8 1.0 C A:HIS87 4.5 40.2 1.0 CA A:HIS421 4.7 45.9 1.0 O A:HOH748 4.7 40.0 1.0 O A:HOH827 4.8 51.5 1.0 CB A:HIS421 4.8 43.7 1.0 N A:HIS87 4.9 38.6 1.0

Copper binding site 4 out of 4 in the Crystal Structure of Laccases From Pycnoporus Sanguineus, Izoform I


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 4 of Crystal Structure of Laccases From Pycnoporus Sanguineus, Izoform I within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu620 b:50.6 occ:1.00 ND1 A:HIS477 2.0 46.0 1.0 SG A:CYS472 2.1 49.4 1.0 ND1 A:HIS416 2.2 52.5 1.0 CG A:HIS477 3.0 49.3 1.0 CE1 A:HIS477 3.0 45.7 1.0 CG A:HIS416 3.1 52.5 1.0 CE1 A:HIS416 3.1 52.3 1.0 CB A:CYS472 3.2 44.1 1.0 CB A:HIS477 3.3 49.0 1.0 CB A:HIS416 3.4 54.5 1.0 CD2 A:PHE482 3.5 51.6 1.0 CD1 A:ILE474 3.5 44.9 1.0 CE2 A:PHE482 3.7 48.5 1.0 CB A:ILE474 3.9 44.8 1.0 CA A:HIS416 4.0 52.2 1.0 NE2 A:HIS477 4.1 48.0 1.0 CD2 A:HIS477 4.1 49.2 1.0 CG1 A:ILE474 4.2 44.1 1.0 NE2 A:HIS416 4.2 56.2 1.0 CD2 A:HIS416 4.2 54.7 1.0 CA A:CYS472 4.6 46.4 1.0 CG2 A:ILE474 4.8 45.3 1.0 CG A:PHE482 4.8 50.5 1.0 CD A:PRO417 4.9 46.1 1.0 CA A:HIS477 4.9 49.4 1.0 N A:ILE474 4.9 44.8 1.0 O A:GLY413 4.9 55.1 1.0 CA A:ILE474 5.0 44.0 1.0 O A:ILE474 5.0 43.0 1.0

M.Orlikowska, M.De J Rostro-Alanis, A.Bujacz, C.Hernandez-Luna, R.Rubio, R.Parra, G.Bujacz. Structural Studies of Two Thermostable Laccases From the White-Rot Fungus Pycnoporus Sanguineus. Int. J. Biol. Macromol. V. 107 1629 2018.
ISSN: ISSN 1879-0003
PubMed: 29055703
DOI: 10.1016/J.IJBIOMAC.2017.10.024