Copper in PDB 5ldu: Recombinant High-Redox Potential Laccase From Basidiomycete Trametes Hirsuta

The structure of Recombinant High-Redox Potential Laccase From Basidiomycete Trametes Hirsuta, PDB code: 5ldu was solved by K.M.Polyakov, T.V.Fedorova, D.S.Loginov, O.S.Savinova, V.S.Lamzin, O.V.Koroleva, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	10.00 / 2.30
Space group	P 65
Cell size a, b, c (Å), α, β, γ (°)	160.650, 160.650, 53.460, 90.00, 90.00, 120.00
R / Rfree (%)	16.4 / 21

The structure of Recombinant High-Redox Potential Laccase From Basidiomycete Trametes Hirsuta also contains other interesting chemical elements:

Sodium

(Na)

2 atoms

The binding sites of Copper atom in the Recombinant High-Redox Potential Laccase From Basidiomycete Trametes Hirsuta (pdb code 5ldu). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 4 binding sites of Copper where determined in the Recombinant High-Redox Potential Laccase From Basidiomycete Trametes Hirsuta, PDB code: 5ldu:
Jump to Copper binding site number: 1; 2; 3; 4;

Copper binding site 1 out of 4 in the Recombinant High-Redox Potential Laccase From Basidiomycete Trametes Hirsuta


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of Recombinant High-Redox Potential Laccase From Basidiomycete Trametes Hirsuta within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu501 b:17.8 occ:0.80 O A:HOH689 2.0 18.0 1.0 NE2 A:HIS400 2.0 16.6 1.0 NE2 A:HIS111 2.0 17.5 1.0 NE2 A:HIS452 2.0 22.0 1.0 CE1 A:HIS400 2.7 14.9 1.0 CE1 A:HIS111 3.0 18.8 1.0 CE1 A:HIS452 3.0 23.1 1.0 CD2 A:HIS111 3.0 17.9 1.0 CD2 A:HIS452 3.0 21.6 1.0 ND1 A:HIS398 3.1 15.4 0.5 CD2 A:HIS400 3.2 14.5 1.0 CE1 A:HIS398 3.2 15.2 0.5 O A:HOH846 3.5 31.6 1.0 ND1 A:HIS400 4.0 15.4 1.0 CD2 A:HIS398 4.0 14.2 0.5 ND1 A:HIS111 4.1 18.1 1.0 CU A:CU503 4.1 14.8 0.5 ND1 A:HIS452 4.1 21.7 1.0 CG A:HIS452 4.2 20.6 1.0 CG A:HIS111 4.2 19.3 1.0 CG A:HIS400 4.2 14.8 1.0 CD2 A:HIS64 4.4 20.1 1.0 CU A:CU502 4.4 20.4 0.8 CG A:HIS398 4.4 14.8 0.5 NE2 A:HIS398 4.5 15.5 0.5 NE2 A:HIS454 4.5 21.3 1.0 CD2 A:HIS454 4.6 19.6 1.0 NE2 A:HIS64 4.6 18.6 1.0 CD2 A:PHE450 4.6 13.8 1.0 CE1 A:HIS109 4.7 16.9 1.0 CB A:PHE450 4.9 13.8 1.0 NE2 A:HIS398 4.9 14.2 0.5 CG A:PRO79 4.9 16.8 1.0 CG A:HIS398 4.9 14.3 0.5

Copper binding site 2 out of 4 in the Recombinant High-Redox Potential Laccase From Basidiomycete Trametes Hirsuta


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of Recombinant High-Redox Potential Laccase From Basidiomycete Trametes Hirsuta within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu502 b:20.4 occ:0.80 ND1 A:HIS66 2.0 18.8 1.0 NE2 A:HIS454 2.0 21.3 1.0 NE2 A:HIS109 2.1 18.5 1.0 O A:HOH689 2.5 18.0 1.0 CE1 A:HIS66 2.8 20.1 1.0 CE1 A:HIS454 2.9 18.3 1.0 CE1 A:HIS109 3.0 16.9 1.0 CD2 A:HIS109 3.1 19.1 1.0 CD2 A:HIS454 3.1 19.6 1.0 CG A:HIS66 3.1 18.6 1.0 CB A:HIS66 3.6 18.5 1.0 CE1 A:HIS398 3.7 15.2 0.5 CZ2 A:TRP107 3.8 15.9 1.0 CD2 A:HIS64 3.8 20.1 1.0 ND1 A:HIS398 3.9 15.4 0.5 NE2 A:HIS66 4.0 19.0 1.0 CU A:CU503 4.0 14.8 0.5 O A:HOH846 4.1 31.6 1.0 ND1 A:HIS454 4.1 20.1 1.0 CE2 A:TRP107 4.1 15.8 1.0 ND1 A:HIS109 4.2 17.8 1.0 CD2 A:HIS66 4.2 19.0 1.0 CG A:HIS454 4.2 20.2 1.0 CG A:HIS109 4.2 17.6 1.0 NE1 A:TRP107 4.3 15.9 1.0 NE2 A:HIS64 4.3 18.6 1.0 CD2 A:HIS398 4.3 14.2 0.5 CU A:CU501 4.4 17.8 0.8 CB A:ALA241 4.5 18.2 1.0 NE2 A:HIS398 4.5 15.5 0.5 CH2 A:TRP107 4.5 15.5 1.0 NE2 A:HIS398 4.7 14.2 0.5 CA A:HIS66 4.7 17.6 1.0 CG A:HIS398 4.9 14.8 0.5

Copper binding site 3 out of 4 in the Recombinant High-Redox Potential Laccase From Basidiomycete Trametes Hirsuta


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 3 of Recombinant High-Redox Potential Laccase From Basidiomycete Trametes Hirsuta within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu503 b:14.8 occ:0.50 CE1 A:HIS398 1.8 15.2 0.5 NE2 A:HIS398 1.9 15.5 0.5 NE2 A:HIS64 1.9 18.6 1.0 NE2 A:HIS398 2.2 14.2 0.5 O A:HOH780 2.3 18.8 1.0 CD2 A:HIS398 2.7 14.2 0.5 CE1 A:HIS64 2.8 20.9 1.0 CD2 A:HIS64 2.9 20.1 1.0 ND1 A:HIS398 3.1 15.4 0.5 CD2 A:HIS398 3.3 15.2 0.5 CE1 A:HIS398 3.4 13.8 0.5 NE2 A:HIS400 3.4 16.6 1.0 CE1 A:HIS400 3.4 14.9 1.0 ND1 A:HIS66 3.5 18.8 1.0 CD2 A:HIS400 3.6 14.5 1.0 ND1 A:HIS400 3.6 15.4 1.0 CG A:HIS400 3.7 14.8 1.0 CG A:HIS66 3.7 18.6 1.0 CE1 A:HIS66 3.8 20.1 1.0 CG A:HIS398 3.8 14.8 0.5 CA A:HIS66 3.9 17.6 1.0 O A:HOH689 3.9 18.0 1.0 ND1 A:HIS64 3.9 20.9 1.0 CG A:HIS398 4.0 14.3 0.5 CU A:CU502 4.0 20.4 0.8 N A:GLY67 4.0 17.4 1.0 CG A:HIS64 4.0 21.3 1.0 CD2 A:HIS66 4.1 19.0 1.0 CU A:CU501 4.1 17.8 0.8 NE2 A:HIS66 4.2 19.0 1.0 CB A:HIS66 4.2 18.5 1.0 ND1 A:HIS398 4.3 14.6 0.5 O A:HOH697 4.4 18.2 1.0 C A:HIS66 4.5 17.6 1.0 CA A:HIS400 4.5 16.4 1.0 O A:HOH756 4.5 13.1 1.0 CB A:HIS400 4.6 16.0 1.0 N A:HIS66 5.0 18.5 1.0

Copper binding site 4 out of 4 in the Recombinant High-Redox Potential Laccase From Basidiomycete Trametes Hirsuta


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 4 of Recombinant High-Redox Potential Laccase From Basidiomycete Trametes Hirsuta within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu504 b:21.4 occ:1.00 ND1 A:HIS458 2.0 20.2 1.0 ND1 A:HIS395 2.0 18.9 1.0 SG A:CYS453 2.3 21.6 1.0 CG A:HIS458 3.0 20.5 1.0 CE1 A:HIS458 3.0 20.6 1.0 CE1 A:HIS395 3.0 17.6 1.0 CG A:HIS395 3.0 19.1 1.0 CB A:HIS458 3.3 19.8 1.0 CB A:CYS453 3.3 19.5 1.0 CB A:HIS395 3.4 19.9 1.0 CD1 A:ILE455 3.7 17.1 1.0 CD2 A:PHE463 3.8 19.3 1.0 CB A:ILE455 3.9 18.3 1.0 CA A:HIS395 3.9 20.6 1.0 CE2 A:PHE463 4.1 19.1 1.0 NE2 A:HIS458 4.1 20.8 1.0 NE2 A:HIS395 4.1 19.1 1.0 CD2 A:HIS458 4.1 18.7 1.0 CG1 A:ILE455 4.1 18.3 1.0 CD2 A:HIS395 4.2 18.1 1.0 O A:GLY392 4.6 23.3 1.0 CG2 A:ILE455 4.7 16.4 1.0 CD A:PRO396 4.7 17.9 1.0 CA A:CYS453 4.7 18.0 1.0 N A:ILE455 4.8 19.0 1.0 CA A:HIS458 4.9 22.5 1.0 CA A:ILE455 4.9 20.0 1.0 C A:HIS395 4.9 19.1 1.0 N A:HIS395 5.0 22.1 1.0

T.V.Fedorova, K.M.Polyakov, D.S.Loginov, O.S.Savinova, E.A.Vavilova, A.M.Chulkin, V.S.Lamzin, O.V.Koroleva. First Structure of Recombinant High-Redox Potential Laccase From Basidiomycete Trametes Hirsuta. To Be Published.