Copper in PDB 5i6o: Crystal Structure of Copper Nitrite Reductase at 100K After 20.70 Mgy

All present enzymatic activity of Crystal Structure of Copper Nitrite Reductase at 100K After 20.70 Mgy:
1.7.2.1;

The structure of Crystal Structure of Copper Nitrite Reductase at 100K After 20.70 Mgy, PDB code: 5i6o was solved by S.Horrell, M.A.Hough, R.W.Strange, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	42.60 / 1.45
Space group	P 21 3
Cell size a, b, c (Å), α, β, γ (°)	95.236, 95.236, 95.236, 90.00, 90.00, 90.00
R / Rfree (%)	17.1 / 19.3

The binding sites of Copper atom in the Crystal Structure of Copper Nitrite Reductase at 100K After 20.70 Mgy (pdb code 5i6o). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 2 binding sites of Copper where determined in the Crystal Structure of Copper Nitrite Reductase at 100K After 20.70 Mgy, PDB code: 5i6o:
Jump to Copper binding site number: 1; 2;

Copper binding site 1 out of 2 in the Crystal Structure of Copper Nitrite Reductase at 100K After 20.70 Mgy


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of Crystal Structure of Copper Nitrite Reductase at 100K After 20.70 Mgy within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu501 b:19.4 occ:1.00 ND1 A:HIS145 2.0 19.5 1.0 ND1 A:HIS95 2.0 16.9 1.0 SG A:CYS136 2.2 18.2 1.0 SD A:MET150 2.4 22.3 1.0 CE1 A:HIS145 2.9 19.5 1.0 CE1 A:HIS95 3.0 18.0 1.0 CG A:HIS95 3.1 17.2 1.0 CG A:HIS145 3.1 18.1 1.0 CB A:CYS136 3.2 17.6 1.0 CE A:MET150 3.2 23.0 1.0 CB A:HIS95 3.5 17.3 1.0 CB A:HIS145 3.5 17.9 1.0 CA A:HIS95 3.9 17.1 1.0 CG A:MET150 3.9 22.0 1.0 NE2 A:HIS145 4.1 20.3 1.0 NE2 A:HIS95 4.1 17.9 1.0 CD2 A:HIS145 4.2 20.8 1.0 CD2 A:HIS95 4.2 17.9 1.0 CG A:PRO138 4.2 23.4 1.0 O A:LEU94 4.2 20.6 1.0 CB A:MET150 4.3 18.7 1.0 SD A:MET62 4.5 25.1 1.0 CD A:PRO138 4.6 20.9 1.0 CA A:CYS136 4.6 17.1 1.0 N A:ASN96 4.7 17.0 1.0 CA A:HIS145 4.7 17.1 1.0 N A:HIS95 4.8 18.4 1.0 C A:HIS95 4.9 18.2 1.0 CB A:MET62 4.9 22.3 1.0 C A:LEU94 4.9 20.7 1.0

Copper binding site 2 out of 2 in the Crystal Structure of Copper Nitrite Reductase at 100K After 20.70 Mgy


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of Crystal Structure of Copper Nitrite Reductase at 100K After 20.70 Mgy within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu502 b:18.4 occ:1.00 O A:NO503 1.8 36.8 0.6 NE2 A:HIS100 2.0 16.4 1.0 NE2 A:HIS135 2.0 16.3 1.0 N A:NO503 2.2 29.8 0.6 CE1 A:HIS100 2.9 16.6 1.0 CD2 A:HIS135 3.0 16.1 1.0 CE1 A:HIS135 3.1 16.4 1.0 CD2 A:HIS100 3.1 16.3 1.0 OD2 A:ASP98 3.9 24.0 0.5 ND1 A:HIS100 4.1 16.7 1.0 ND1 A:HIS135 4.2 16.4 1.0 CG A:HIS135 4.2 16.6 1.0 CG A:HIS100 4.2 15.2 1.0 O A:HOH788 4.2 18.2 0.3 CG A:ASP98 4.6 20.9 0.5 OD1 A:ASP98 4.9 22.6 0.5

S.Horrell, S.V.Antonyuk, R.R.Eady, S.S.Hasnain, M.A.Hough, R.W.Strange. Serial Crystallography Captures Enzyme Catalysis in Copper Nitrite Reductase at Atomic Resolution From One Crystal. Iucrj V. 3 271 2016.
ISSN: ESSN 2052-2525
PubMed: 27437114
DOI: 10.1107/S205225251600823X