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Copper in PDB 5b1a: Bovine Heart Cytochrome C Oxidase in the Fully Oxidized State at 1.5 Angstrom Resolution

Enzymatic activity of Bovine Heart Cytochrome C Oxidase in the Fully Oxidized State at 1.5 Angstrom Resolution

All present enzymatic activity of Bovine Heart Cytochrome C Oxidase in the Fully Oxidized State at 1.5 Angstrom Resolution:
1.9.3.1;

Protein crystallography data

The structure of Bovine Heart Cytochrome C Oxidase in the Fully Oxidized State at 1.5 Angstrom Resolution, PDB code: 5b1a was solved by N.Yano, K.Muramoto, A.Shimada, S.Takemura, J.Baba, H.Fujisawa, M.Mochizuki, K.Shinzawa-Itoh, E.Yamashita, T.Tsukihara, S.Yoshikawa, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 40.00 / 1.50
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 181.938, 204.400, 177.896, 90.00, 90.00, 90.00
R / Rfree (%) 14.9 / 17.2

Other elements in 5b1a:

The structure of Bovine Heart Cytochrome C Oxidase in the Fully Oxidized State at 1.5 Angstrom Resolution also contains other interesting chemical elements:

Magnesium (Mg) 2 atoms
Zinc (Zn) 2 atoms
Iron (Fe) 4 atoms
Sodium (Na) 2 atoms

Copper Binding Sites:

The binding sites of Copper atom in the Bovine Heart Cytochrome C Oxidase in the Fully Oxidized State at 1.5 Angstrom Resolution (pdb code 5b1a). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 6 binding sites of Copper where determined in the Bovine Heart Cytochrome C Oxidase in the Fully Oxidized State at 1.5 Angstrom Resolution, PDB code: 5b1a:
Jump to Copper binding site number: 1; 2; 3; 4; 5; 6;

Copper binding site 1 out of 6 in 5b1a

Go back to Copper Binding Sites List in 5b1a
Copper binding site 1 out of 6 in the Bovine Heart Cytochrome C Oxidase in the Fully Oxidized State at 1.5 Angstrom Resolution


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of Bovine Heart Cytochrome C Oxidase in the Fully Oxidized State at 1.5 Angstrom Resolution within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cu603

b:19.0
occ:1.00
 ND1 A:HIS240 2.0 17.2 1.0
NE2 A:HIS291 2.0 17.7 1.0
NE2 A:HIS290 2.0 18.3 1.0
O2 A:PER606 2.2 22.5 1.0
O1 A:PER606 2.7 17.6 1.0
CE1 A:HIS291 2.9 18.4 1.0
CE1 A:HIS240 3.0 18.5 1.0
CD2 A:HIS291 3.0 17.6 1.0
CE1 A:HIS290 3.0 19.3 1.0
CG A:HIS240 3.0 17.7 1.0
CD2 A:HIS290 3.1 19.0 1.0
CB A:HIS240 3.4 17.3 1.0
CA A:HIS240 3.9 17.8 1.0
ND1 A:HIS291 4.1 18.4 1.0
CG A:HIS291 4.1 17.9 1.0
NE2 A:HIS240 4.1 16.8 1.0
CD2 A:HIS240 4.2 17.5 1.0
ND1 A:HIS290 4.2 18.6 1.0
CG A:HIS290 4.2 19.0 1.0
NA A:HEA602 4.5 17.7 1.0
C1A A:HEA602 4.6 17.9 1.0
C4A A:HEA602 4.7 18.6 1.0
N A:HIS240 4.7 17.5 1.0
C2A A:HEA602 4.8 18.0 1.0
FE A:HEA602 4.8 18.4 1.0
CG2 A:VAL243 4.9 18.4 1.0
C3A A:HEA602 4.9 17.5 1.0
CHA A:HEA602 5.0 18.7 1.0
C A:HIS240 5.0 18.2 1.0

Copper binding site 2 out of 6 in 5b1a

Go back to Copper Binding Sites List in 5b1a
Copper binding site 2 out of 6 in the Bovine Heart Cytochrome C Oxidase in the Fully Oxidized State at 1.5 Angstrom Resolution


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of Bovine Heart Cytochrome C Oxidase in the Fully Oxidized State at 1.5 Angstrom Resolution within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Cu302

b:21.4
occ:1.00
 CU1 B:CUA302 0.0 21.4 1.0
ND1 B:HIS161 2.1 21.0 1.0
SG B:CYS196 2.3 20.3 1.0
SG B:CYS200 2.4 20.7 1.0
SD B:MET207 2.4 21.8 1.0
CU2 B:CUA302 2.5 21.2 1.0
CE1 B:HIS161 3.0 20.0 1.0
CE B:MET207 3.2 21.9 1.0
CG B:HIS161 3.2 21.2 1.0
CB B:CYS200 3.2 20.5 1.0
CB B:CYS196 3.4 21.0 1.0
CG B:MET207 3.5 21.2 1.0
CB B:HIS161 3.6 19.2 1.0
O B:GLU198 4.1 19.5 1.0
NE2 B:HIS161 4.1 20.4 1.0
CA B:HIS161 4.2 19.5 1.0
CD2 B:HIS161 4.3 19.8 1.0
ND1 B:HIS204 4.4 19.3 1.0
O B:HIS102 4.7 22.2 1.0
CA B:CYS200 4.7 20.4 1.0
CA B:HIS204 4.7 19.3 1.0
CD1 B:TRP104 4.7 22.2 1.0
O B:LEU160 4.7 21.0 1.0
CA B:CYS196 4.8 20.0 1.0
O B:HIS204 4.9 22.6 1.0
CB B:MET207 4.9 21.4 1.0
N B:CYS200 5.0 20.4 1.0

Copper binding site 3 out of 6 in 5b1a

Go back to Copper Binding Sites List in 5b1a
Copper binding site 3 out of 6 in the Bovine Heart Cytochrome C Oxidase in the Fully Oxidized State at 1.5 Angstrom Resolution


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 3 of Bovine Heart Cytochrome C Oxidase in the Fully Oxidized State at 1.5 Angstrom Resolution within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Cu302

b:21.2
occ:1.00
 CU2 B:CUA302 0.0 21.2 1.0
ND1 B:HIS204 2.0 19.3 1.0
SG B:CYS196 2.3 20.3 1.0
SG B:CYS200 2.3 20.7 1.0
O B:GLU198 2.5 19.5 1.0
CU1 B:CUA302 2.5 21.4 1.0
CE1 B:HIS204 2.9 20.2 1.0
CG B:HIS204 3.1 21.8 1.0
CB B:CYS196 3.3 21.0 1.0
CB B:CYS200 3.3 20.5 1.0
C B:GLU198 3.5 19.2 1.0
CB B:HIS204 3.5 19.5 1.0
CA B:HIS204 3.6 19.3 1.0
N B:CYS200 3.7 20.4 1.0
O B:HIS204 3.8 22.6 1.0
N B:GLU198 4.1 19.9 1.0
NE2 B:HIS204 4.1 20.9 1.0
CA B:CYS200 4.1 20.4 1.0
C B:HIS204 4.2 20.6 1.0
CD2 B:HIS204 4.2 20.7 1.0
ND1 B:HIS161 4.2 21.0 1.0
C B:ILE199 4.2 19.6 1.0
CA B:ILE199 4.2 20.4 1.0
C B:CYS196 4.2 18.6 1.0
O B:CYS196 4.3 20.2 1.0
SD B:MET207 4.3 21.8 1.0
N B:ILE199 4.3 20.1 1.0
CA B:CYS196 4.4 20.0 1.0
CA B:GLU198 4.5 19.1 1.0
N B:SER197 4.7 19.2 1.0
CG B:MET207 4.7 21.2 1.0
N B:HIS204 4.8 20.5 1.0
CA B:HIS161 4.9 19.5 1.0

Copper binding site 4 out of 6 in 5b1a

Go back to Copper Binding Sites List in 5b1a
Copper binding site 4 out of 6 in the Bovine Heart Cytochrome C Oxidase in the Fully Oxidized State at 1.5 Angstrom Resolution


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 4 of Bovine Heart Cytochrome C Oxidase in the Fully Oxidized State at 1.5 Angstrom Resolution within 5.0Å range:
probe atom residue distance (Å) B Occ
N:Cu603

b:21.7
occ:1.00
 NE2 N:HIS291 2.0 21.8 1.0
ND1 N:HIS240 2.0 19.1 1.0
NE2 N:HIS290 2.0 21.5 1.0
O2 N:PER606 2.2 26.2 1.0
O1 N:PER606 2.7 20.7 1.0
CE1 N:HIS291 2.9 21.8 1.0
CE1 N:HIS240 3.0 20.5 1.0
CE1 N:HIS290 3.0 21.6 1.0
CD2 N:HIS291 3.0 22.9 1.0
CG N:HIS240 3.0 19.1 1.0
CD2 N:HIS290 3.1 21.3 1.0
CB N:HIS240 3.4 19.8 1.0
CA N:HIS240 3.9 20.1 1.0
ND1 N:HIS291 4.1 20.9 1.0
CG N:HIS291 4.1 21.1 1.0
NE2 N:HIS240 4.1 20.1 1.0
ND1 N:HIS290 4.1 21.1 1.0
CD2 N:HIS240 4.2 19.5 1.0
CG N:HIS290 4.2 21.4 1.0
NA N:HEA602 4.5 20.2 1.0
C1A N:HEA602 4.5 22.0 1.0
C4A N:HEA602 4.6 21.1 1.0
N N:HIS240 4.7 19.5 1.0
FE N:HEA602 4.8 21.2 1.0
C2A N:HEA602 4.9 21.0 1.0
C3A N:HEA602 4.9 21.3 1.0
CG2 N:VAL243 4.9 19.9 1.0
CHA N:HEA602 5.0 20.6 1.0

Copper binding site 5 out of 6 in 5b1a

Go back to Copper Binding Sites List in 5b1a
Copper binding site 5 out of 6 in the Bovine Heart Cytochrome C Oxidase in the Fully Oxidized State at 1.5 Angstrom Resolution


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 5 of Bovine Heart Cytochrome C Oxidase in the Fully Oxidized State at 1.5 Angstrom Resolution within 5.0Å range:
probe atom residue distance (Å) B Occ
O:Cu301

b:27.7
occ:1.00
 CU1 O:CUA301 0.0 27.7 1.0
ND1 O:HIS161 2.1 26.8 1.0
SG O:CYS196 2.3 26.8 1.0
SG O:CYS200 2.3 27.1 1.0
SD O:MET207 2.4 28.5 1.0
CU2 O:CUA301 2.5 27.4 1.0
CE1 O:HIS161 3.0 26.9 1.0
CE O:MET207 3.2 27.9 1.0
CG O:HIS161 3.2 26.6 1.0
CB O:CYS200 3.3 25.7 1.0
CB O:CYS196 3.4 26.6 1.0
CG O:MET207 3.5 28.2 1.0
CB O:HIS161 3.6 26.1 1.0
O O:GLU198 4.1 26.1 1.0
NE2 O:HIS161 4.1 27.3 1.0
CA O:HIS161 4.2 25.7 1.0
CD2 O:HIS161 4.3 27.5 1.0
ND1 O:HIS204 4.4 24.5 1.0
CD1 O:TRP104 4.6 28.4 1.0
CA O:CYS200 4.7 26.7 1.0
O O:HIS102 4.7 30.0 1.0
O O:LEU160 4.7 26.2 1.0
CA O:HIS204 4.7 27.0 1.0
CA O:CYS196 4.8 26.6 1.0
O O:HIS204 4.9 27.4 1.0
CB O:MET207 4.9 28.5 1.0

Copper binding site 6 out of 6 in 5b1a

Go back to Copper Binding Sites List in 5b1a
Copper binding site 6 out of 6 in the Bovine Heart Cytochrome C Oxidase in the Fully Oxidized State at 1.5 Angstrom Resolution


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 6 of Bovine Heart Cytochrome C Oxidase in the Fully Oxidized State at 1.5 Angstrom Resolution within 5.0Å range:
probe atom residue distance (Å) B Occ
O:Cu301

b:27.4
occ:1.00
 CU2 O:CUA301 0.0 27.4 1.0
ND1 O:HIS204 2.0 24.5 1.0
SG O:CYS200 2.3 27.1 1.0
SG O:CYS196 2.3 26.8 1.0
O O:GLU198 2.5 26.1 1.0
CU1 O:CUA301 2.5 27.7 1.0
CE1 O:HIS204 2.9 25.3 1.0
CG O:HIS204 3.1 26.7 1.0
CB O:CYS196 3.3 26.6 1.0
CB O:CYS200 3.3 25.7 1.0
C O:GLU198 3.5 24.9 1.0
CB O:HIS204 3.6 26.9 1.0
CA O:HIS204 3.6 27.0 1.0
N O:CYS200 3.7 25.6 1.0
O O:HIS204 3.8 27.4 1.0
N O:GLU198 4.1 25.5 1.0
NE2 O:HIS204 4.1 25.5 1.0
CA O:CYS200 4.1 26.7 1.0
C O:HIS204 4.2 27.3 1.0
CD2 O:HIS204 4.2 26.0 1.0
ND1 O:HIS161 4.2 26.8 1.0
CA O:ILE199 4.2 24.8 1.0
O O:CYS196 4.3 27.4 1.0
C O:CYS196 4.3 27.5 1.0
C O:ILE199 4.3 25.2 1.0
SD O:MET207 4.3 28.5 1.0
CA O:CYS196 4.3 26.6 1.0
N O:ILE199 4.4 24.1 1.0
CA O:GLU198 4.5 24.8 1.0
N O:SER197 4.7 26.2 1.0
CG O:MET207 4.7 28.2 1.0
N O:HIS204 4.8 28.1 1.0
CA O:HIS161 4.9 25.7 1.0

Reference:

N.Yano, K.Muramoto, A.Shimada, S.Takemura, J.Baba, H.Fujisawa, M.Mochizuki, K.Shinzawa-Itoh, E.Yamashita, T.Tsukihara, S.Yoshikawa. The MG2+-Containing Water Cluster of Mammalian Cytochrome C Oxidase Collects Four Pumping Proton Equivalents in Each Catalytic Cycle. J.Biol.Chem. V. 291 23882 2016.
ISSN: ESSN 1083-351X
PubMed: 27605664
DOI: 10.1074/JBC.M115.711770
Page generated: Wed Jul 31 03:51:07 2024

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