Copper in PDB 5a7e: Crystallographic Structural Determination of A Trigonal Laccase From Coriolopsis Gallica (Cgl) to 1.5 A Resolution

The structure of Crystallographic Structural Determination of A Trigonal Laccase From Coriolopsis Gallica (Cgl) to 1.5 A Resolution, PDB code: 5a7e was solved by R.R.Ruiz-Arellano, A.Diaz, M.Ayala, E.De La Mora, E.Rudino-Pinera, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	43.08 / 1.50
Space group	P 1
Cell size a, b, c (Å), α, β, γ (°)	45.742, 56.406, 59.173, 75.67, 73.03, 76.49
R / Rfree (%)	16.3 / 18.3

The binding sites of Copper atom in the Crystallographic Structural Determination of A Trigonal Laccase From Coriolopsis Gallica (Cgl) to 1.5 A Resolution (pdb code 5a7e). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 4 binding sites of Copper where determined in the Crystallographic Structural Determination of A Trigonal Laccase From Coriolopsis Gallica (Cgl) to 1.5 A Resolution, PDB code: 5a7e:
Jump to Copper binding site number: 1; 2; 3; 4;

Copper binding site 1 out of 4 in the Crystallographic Structural Determination of A Trigonal Laccase From Coriolopsis Gallica (Cgl) to 1.5 A Resolution


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of Crystallographic Structural Determination of A Trigonal Laccase From Coriolopsis Gallica (Cgl) to 1.5 A Resolution within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu1496 b:12.7 occ:1.00 ND1 A:HIS65 2.0 9.1 1.0 NE2 A:HIS108 2.1 8.9 1.0 NE2 A:HIS450 2.2 10.7 1.0 CE1 A:HIS65 2.8 10.4 1.0 CE1 A:HIS450 3.0 10.5 1.0 CD2 A:HIS108 3.0 8.1 1.0 CG A:HIS65 3.1 9.5 1.0 CE1 A:HIS108 3.1 10.8 1.0 CD2 A:HIS450 3.3 11.7 1.0 CB A:HIS65 3.5 8.8 1.0 CZ2 A:TRP106 3.6 10.2 1.0 CD2 A:HIS63 3.7 10.7 1.0 CE2 A:TRP106 3.9 8.8 1.0 NE2 A:HIS65 4.0 11.5 1.0 NE1 A:TRP106 4.0 9.3 1.0 CU A:CU1497 4.1 12.4 0.8 CD2 A:HIS65 4.1 10.8 1.0 ND1 A:HIS450 4.1 10.8 1.0 ND1 A:HIS108 4.2 9.0 1.0 CG A:HIS108 4.2 7.4 1.0 NE2 A:HIS63 4.2 9.3 1.0 CH2 A:TRP106 4.3 10.6 1.0 CG A:HIS450 4.3 8.9 1.0 CB A:ALA239 4.4 10.3 1.0 CD2 A:HIS396 4.4 10.9 1.0 NE2 A:HIS396 4.5 11.0 1.0 O A:HOH2195 4.6 29.3 1.0 CA A:HIS65 4.7 9.7 1.0 CD2 A:TRP106 4.8 7.5 1.0 CG A:HIS63 4.9 9.8 1.0 CD1 A:TRP106 5.0 11.1 1.0

Copper binding site 2 out of 4 in the Crystallographic Structural Determination of A Trigonal Laccase From Coriolopsis Gallica (Cgl) to 1.5 A Resolution


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of Crystallographic Structural Determination of A Trigonal Laccase From Coriolopsis Gallica (Cgl) to 1.5 A Resolution within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu1497 b:12.4 occ:0.80 NE2 A:HIS396 1.9 11.0 1.0 NE2 A:HIS63 1.9 9.3 1.0 O A:HOH2128 2.7 14.7 1.0 CE1 A:HIS63 2.8 11.0 1.0 CE1 A:HIS396 2.9 11.2 1.0 CD2 A:HIS396 2.9 10.9 1.0 CD2 A:HIS63 2.9 10.7 1.0 NE2 A:HIS398 3.4 12.7 1.0 ND1 A:HIS65 3.4 9.1 1.0 CE1 A:HIS398 3.6 11.6 1.0 CG A:HIS65 3.6 9.5 1.0 CD2 A:HIS398 3.6 12.6 1.0 CE1 A:HIS65 3.7 10.4 1.0 ND1 A:HIS398 3.7 13.2 1.0 CG A:HIS398 3.8 10.7 1.0 ND1 A:HIS63 3.9 11.8 1.0 ND1 A:HIS396 4.0 11.0 1.0 CA A:HIS65 4.0 9.7 1.0 CG A:HIS63 4.0 9.8 1.0 CG A:HIS396 4.0 11.1 1.0 CD2 A:HIS65 4.0 10.8 1.0 CU A:CU1496 4.1 12.7 1.0 NE2 A:HIS65 4.1 11.5 1.0 CB A:HIS65 4.1 8.8 1.0 N A:GLY66 4.2 11.1 1.0 CU A:CU1499 4.5 15.8 1.0 O A:HOH2135 4.6 14.9 1.0 CA A:HIS398 4.6 11.8 1.0 C A:HIS65 4.6 11.0 1.0 CB A:HIS398 4.7 12.9 1.0 O A:HOH2482 4.7 18.3 1.0 N A:HIS65 5.0 8.0 1.0

Copper binding site 3 out of 4 in the Crystallographic Structural Determination of A Trigonal Laccase From Coriolopsis Gallica (Cgl) to 1.5 A Resolution


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 3 of Crystallographic Structural Determination of A Trigonal Laccase From Coriolopsis Gallica (Cgl) to 1.5 A Resolution within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu1498 b:15.7 occ:0.85 ND1 A:HIS454 2.1 20.7 1.0 ND1 A:HIS393 2.1 14.3 1.0 SG A:CYS449 2.2 14.8 1.0 CE1 A:HIS393 3.0 23.9 1.0 CE1 A:HIS454 3.1 16.3 1.0 CG A:HIS393 3.1 13.4 1.0 CG A:HIS454 3.1 15.5 1.0 CB A:CYS449 3.3 16.0 1.0 CB A:HIS454 3.4 15.7 1.0 CB A:HIS393 3.4 17.8 1.0 CD1 A:ILE451 3.6 15.0 1.0 CD2 A:PHE459 3.8 16.4 1.0 CB A:ILE451 3.8 12.8 1.0 CA A:HIS393 4.0 16.6 1.0 CE2 A:PHE459 4.0 17.6 1.0 CG1 A:ILE451 4.0 14.2 1.0 NE2 A:HIS393 4.1 21.4 1.0 CD2 A:HIS393 4.2 21.2 1.0 NE2 A:HIS454 4.2 18.0 1.0 CD2 A:HIS454 4.2 17.3 1.0 CG2 A:ILE451 4.6 13.9 1.0 CA A:CYS449 4.7 13.4 1.0 O A:GLY390 4.7 24.8 1.0 CD A:PRO394 4.7 14.1 1.0 N A:ILE451 4.8 11.9 1.0 CA A:ILE451 4.9 13.4 1.0 CA A:HIS454 4.9 16.8 1.0 N A:HIS393 4.9 14.8 1.0 C A:HIS393 5.0 15.5 1.0 CG A:PHE459 5.0 18.2 1.0 O A:ILE451 5.0 14.4 1.0

Copper binding site 4 out of 4 in the Crystallographic Structural Determination of A Trigonal Laccase From Coriolopsis Gallica (Cgl) to 1.5 A Resolution


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 4 of Crystallographic Structural Determination of A Trigonal Laccase From Coriolopsis Gallica (Cgl) to 1.5 A Resolution within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu1499 b:15.8 occ:0.98 NE2 A:HIS448 2.0 13.6 1.0 NE2 A:HIS398 2.0 12.7 1.0 NE2 A:HIS110 2.1 12.1 1.0 CE1 A:HIS398 2.8 11.6 1.0 CE1 A:HIS110 2.9 13.5 1.0 CD2 A:HIS448 2.9 16.3 1.0 CE1 A:HIS448 3.0 15.1 1.0 CD2 A:HIS110 3.1 10.7 1.0 CD2 A:HIS398 3.2 12.6 1.0 O A:HOH2195 3.9 29.3 1.0 CD2 A:HIS396 4.0 10.9 1.0 CD2 A:PHE446 4.0 18.2 1.0 ND1 A:HIS398 4.0 13.2 1.0 ND1 A:HIS110 4.1 16.0 1.0 ND1 A:HIS448 4.1 14.1 1.0 CG A:HIS448 4.1 16.2 1.0 CG A:HIS398 4.2 10.7 1.0 CG A:HIS110 4.2 13.0 1.0 CB A:PHE446 4.4 15.6 1.0 CU A:CU1497 4.5 12.4 0.8 CG A:PRO78 4.6 14.3 1.0 CD2 A:HIS63 4.6 10.7 1.0 CG A:PHE446 4.6 15.7 1.0 NE2 A:HIS396 4.6 11.0 1.0 NE2 A:HIS63 4.8 9.3 1.0 CE2 A:PHE446 4.9 29.7 1.0 NE2 A:HIS450 4.9 10.7 1.0

E.De La Mora, N.Morales, G.Rodriguez-Almazan, R.R.Ruiz-Arellano, E.Rudino-Pinira. Metal Site Delocalization Observed in A Model Multicopper Oxidase: An Insight Into Water Release Mechanism To Be Published.