Atomistry » Copper » PDB 4tm7-4yst » 4wtq
Atomistry »
  Copper »
    PDB 4tm7-4yst »
      4wtq »

Copper in PDB 4wtq: Structure of the SSL1 Laccase Mutant M295L

Enzymatic activity of Structure of the SSL1 Laccase Mutant M295L

All present enzymatic activity of Structure of the SSL1 Laccase Mutant M295L:
1.10.3.2;

Protein crystallography data

The structure of Structure of the SSL1 Laccase Mutant M295L, PDB code: 4wtq was solved by M.Gunne, A.Hoeppner, V.B.Urlacher, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 24.58 / 1.80
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 51.528, 104.795, 164.389, 90.00, 90.00, 90.00
R / Rfree (%) 17.1 / 20.8

Copper Binding Sites:

The binding sites of Copper atom in the Structure of the SSL1 Laccase Mutant M295L (pdb code 4wtq). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 9 binding sites of Copper where determined in the Structure of the SSL1 Laccase Mutant M295L, PDB code: 4wtq:
Jump to Copper binding site number: 1; 2; 3; 4; 5; 6; 7; 8; 9;

Copper binding site 1 out of 9 in 4wtq

Go back to Copper Binding Sites List in 4wtq
Copper binding site 1 out of 9 in the Structure of the SSL1 Laccase Mutant M295L


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of Structure of the SSL1 Laccase Mutant M295L within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cu401

b:22.3
occ:1.00
 ND1 A:HIS290 2.0 20.5 1.0
ND1 A:HIS228 2.0 15.8 1.0
SG A:CYS285 2.3 17.3 1.0
CG A:HIS290 3.0 20.4 1.0
CE1 A:HIS290 3.0 21.7 1.0
CG A:HIS228 3.0 17.4 1.0
CE1 A:HIS228 3.1 18.0 1.0
CB A:CYS285 3.1 16.5 1.0
CB A:HIS228 3.3 16.8 1.0
CB A:HIS290 3.3 19.1 1.0
CD2 A:LEU295 3.7 35.8 1.0
CA A:HIS228 3.8 15.5 1.0
NE2 A:HIS290 4.1 22.2 1.0
CD2 A:HIS290 4.1 22.8 1.0
CD2 A:HIS228 4.1 17.8 1.0
CG2 A:VAL287 4.1 15.2 1.0
NE2 A:HIS228 4.1 18.4 1.0
O A:TYR227 4.2 14.9 1.0
CB A:VAL287 4.2 15.5 1.0
CA A:CYS285 4.5 15.0 1.0
N A:THR229 4.6 16.5 1.0
C A:HIS228 4.7 15.0 1.0
N A:HIS228 4.8 15.2 1.0
CA A:HIS290 4.8 19.9 1.0
C A:TYR227 4.9 14.5 1.0
CD2 A:PHE192 4.9 24.6 1.0
CG A:LEU295 5.0 35.5 1.0

Copper binding site 2 out of 9 in 4wtq

Go back to Copper Binding Sites List in 4wtq
Copper binding site 2 out of 9 in the Structure of the SSL1 Laccase Mutant M295L


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of Structure of the SSL1 Laccase Mutant M295L within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cu402

b:23.3
occ:0.90
 NE2 A:HIS101 2.0 18.9 1.0
NE2 B:HIS286 2.0 18.4 1.0
NE2 A:HIS153 2.0 19.8 1.0
CE1 A:HIS101 2.8 19.4 1.0
CE1 B:HIS286 2.8 20.2 1.0
CE1 A:HIS153 2.9 19.9 1.0
CD2 B:HIS286 3.1 19.3 1.0
CD2 A:HIS101 3.1 17.5 1.0
CD2 A:HIS153 3.1 17.9 1.0
O A:O404 3.4 32.9 1.0
CD2 A:HIS99 3.8 31.4 1.0
CB A:ALA263 4.0 20.5 1.0
ND1 B:HIS286 4.0 19.9 1.0
NE2 A:HIS99 4.0 32.8 1.0
ND1 A:HIS101 4.0 18.7 1.0
ND1 A:HIS153 4.1 17.4 1.0
CG B:HIS286 4.1 19.4 1.0
CG A:HIS101 4.1 18.0 1.0
CE1 A:HIS151 4.2 19.6 1.0
CG A:HIS153 4.2 17.7 1.0
NE2 B:HIS231 4.3 27.5 1.0
CD2 B:HIS231 4.4 24.1 1.0
O A:HOH632 4.7 35.0 1.0
CE1 B:HIS231 4.9 23.1 1.0
CE1 A:HIS223 4.9 21.9 1.0
NE2 A:HIS151 4.9 19.5 1.0
ND1 A:HIS151 5.0 19.3 1.0

Copper binding site 3 out of 9 in 4wtq

Go back to Copper Binding Sites List in 4wtq
Copper binding site 3 out of 9 in the Structure of the SSL1 Laccase Mutant M295L


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 3 of Structure of the SSL1 Laccase Mutant M295L within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cu403

b:26.7
occ:0.75
 NE2 A:HIS233 2.1 20.6 1.0
NE2 A:HIS284 2.1 21.5 1.0
NE2 C:HIS155 2.2 18.7 1.0
O C:HOH663 2.3 44.3 1.0
CE1 A:HIS233 2.8 22.9 1.0
CE1 A:HIS284 3.0 22.8 1.0
CD2 A:HIS284 3.2 22.7 1.0
CE1 C:HIS155 3.2 17.9 1.0
CD2 C:HIS155 3.2 18.4 1.0
CD2 A:HIS233 3.3 20.5 1.0
CD2 A:HIS231 3.9 18.4 1.0
SD A:MET282 4.0 43.0 1.0
ND1 A:HIS233 4.0 22.1 1.0
O C:HOH632 4.0 38.0 1.0
ND1 A:HIS284 4.1 19.2 1.0
CG A:MET282 4.2 32.2 1.0
CG A:HIS284 4.2 19.4 1.0
CG A:HIS233 4.3 19.1 1.0
NE2 C:HIS99 4.3 21.2 1.0
NE2 A:HIS231 4.3 20.2 1.0
CD2 C:HIS161 4.3 27.9 1.0
ND1 C:HIS155 4.3 18.3 1.0
CG C:HIS155 4.4 17.0 1.0
CE1 C:HIS99 4.6 22.8 1.0
CD2 C:HIS99 4.8 18.6 1.0
NE2 A:HIS286 4.9 18.5 1.0
NE2 C:HIS161 4.9 33.8 1.0
CE A:MET282 5.0 40.5 1.0

Copper binding site 4 out of 9 in 4wtq

Go back to Copper Binding Sites List in 4wtq
Copper binding site 4 out of 9 in the Structure of the SSL1 Laccase Mutant M295L


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 4 of Structure of the SSL1 Laccase Mutant M295L within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Cu401

b:25.0
occ:1.00
 ND1 B:HIS228 2.0 19.2 1.0
ND1 B:HIS290 2.0 22.7 1.0
SG B:CYS285 2.2 20.6 1.0
CG B:HIS228 2.9 18.1 1.0
CE1 B:HIS228 3.0 16.2 1.0
CG B:HIS290 3.0 23.3 1.0
CE1 B:HIS290 3.0 24.5 1.0
CB B:CYS285 3.1 21.7 1.0
CB B:HIS228 3.3 19.2 1.0
CB B:HIS290 3.3 24.1 1.0
CA B:HIS228 3.6 18.8 1.0
NE2 B:HIS228 4.1 19.7 1.0
CD2 B:HIS228 4.1 18.9 1.0
NE2 B:HIS290 4.1 25.5 1.0
O B:TYR227 4.1 16.8 1.0
CD1 B:LEU295 4.1 49.3 1.0
CD2 B:HIS290 4.1 24.4 1.0
CG2 B:VAL287 4.2 18.8 1.0
CB B:VAL287 4.3 18.3 1.0
CA B:CYS285 4.5 21.3 1.0
N B:THR229 4.5 17.7 1.0
C B:HIS228 4.7 20.1 1.0
N B:HIS228 4.7 17.7 1.0
C B:TYR227 4.8 17.5 1.0
CA B:HIS290 4.9 25.3 1.0
CD2 B:PHE192 4.9 33.5 1.0

Copper binding site 5 out of 9 in 4wtq

Go back to Copper Binding Sites List in 4wtq
Copper binding site 5 out of 9 in the Structure of the SSL1 Laccase Mutant M295L


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 5 of Structure of the SSL1 Laccase Mutant M295L within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Cu402

b:22.7
occ:0.95
 NE2 B:HIS101 2.0 18.1 1.0
NE2 B:HIS153 2.1 20.4 1.0
NE2 C:HIS286 2.1 17.9 1.0
CE1 B:HIS101 2.8 18.7 1.0
CE1 C:HIS286 3.0 19.0 1.0
CE1 B:HIS153 3.0 21.9 1.0
CD2 B:HIS153 3.0 20.1 1.0
CD2 B:HIS101 3.1 19.9 1.0
CD2 C:HIS286 3.1 19.2 1.0
O B:O404 3.3 31.2 1.0
CD2 B:HIS99 3.7 27.6 1.0
CB B:ALA263 4.0 20.6 1.0
ND1 B:HIS101 4.0 19.4 1.0
NE2 B:HIS99 4.1 30.2 1.0
ND1 C:HIS286 4.1 19.2 1.0
CE1 B:HIS151 4.1 19.6 1.0
ND1 B:HIS153 4.1 21.0 1.0
CG B:HIS101 4.1 18.4 1.0
CG B:HIS153 4.2 19.4 1.0
CG C:HIS286 4.2 17.4 1.0
NE2 C:HIS231 4.3 20.7 1.0
CD2 C:HIS231 4.4 19.0 1.0
O B:HOH591 4.6 42.5 1.0
NE2 B:HIS151 4.8 19.8 1.0
CE1 C:HIS231 4.9 18.2 1.0
ND1 B:HIS151 4.9 17.0 1.0
CE1 B:HIS223 5.0 20.5 1.0

Copper binding site 6 out of 9 in 4wtq

Go back to Copper Binding Sites List in 4wtq
Copper binding site 6 out of 9 in the Structure of the SSL1 Laccase Mutant M295L


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 6 of Structure of the SSL1 Laccase Mutant M295L within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Cu403

b:29.3
occ:0.65
 O A:O404 2.0 32.9 1.0
NE2 B:HIS233 2.0 28.6 1.0
NE2 B:HIS284 2.2 27.3 1.0
NE2 A:HIS155 2.2 23.4 1.0
CE1 B:HIS233 2.7 30.6 1.0
CE1 B:HIS284 3.0 27.6 1.0
CE1 A:HIS155 3.0 27.0 1.0
CD2 B:HIS233 3.2 29.8 1.0
CD2 B:HIS284 3.2 25.3 1.0
CD2 A:HIS155 3.3 25.8 1.0
O A:HOH632 3.9 35.0 1.0
ND1 B:HIS233 3.9 29.6 1.0
CD2 B:HIS231 4.0 24.1 1.0
ND1 B:HIS284 4.1 23.8 1.0
CG B:HIS233 4.2 27.8 1.0
ND1 A:HIS155 4.2 27.6 1.0
NE2 A:HIS99 4.2 32.8 1.0
CG B:HIS284 4.3 22.2 1.0
NE2 B:HIS231 4.3 27.5 1.0
CG A:HIS155 4.4 23.9 1.0
CD2 A:HIS161 4.4 36.4 1.0
CE B:MET282 4.5 44.5 1.0
SD B:MET282 4.5 54.5 1.0
CE1 A:HIS99 4.6 30.9 1.0
CD2 A:HIS99 4.6 31.4 1.0
CG B:MET282 4.8 43.6 1.0
NE2 B:HIS286 5.0 18.4 1.0

Copper binding site 7 out of 9 in 4wtq

Go back to Copper Binding Sites List in 4wtq
Copper binding site 7 out of 9 in the Structure of the SSL1 Laccase Mutant M295L


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 7 of Structure of the SSL1 Laccase Mutant M295L within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Cu401

b:21.2
occ:0.95
 NE2 C:HIS101 1.9 15.3 1.0
NE2 A:HIS286 2.1 18.5 1.0
NE2 C:HIS153 2.1 14.4 1.0
CE1 C:HIS101 2.8 15.4 1.0
CE1 C:HIS153 3.0 15.0 1.0
CD2 C:HIS101 3.0 16.0 1.0
CE1 A:HIS286 3.0 17.2 1.0
O C:HOH663 3.0 44.3 1.0
CD2 A:HIS286 3.0 17.2 1.0
CD2 C:HIS153 3.2 15.0 1.0
CD2 C:HIS99 3.8 18.6 1.0
CB C:ALA263 3.9 18.2 1.0
ND1 C:HIS101 4.0 14.2 1.0
CG C:HIS101 4.1 13.8 1.0
ND1 A:HIS286 4.1 18.7 1.0
NE2 C:HIS99 4.1 21.2 1.0
ND1 C:HIS153 4.1 13.7 1.0
CG A:HIS286 4.2 18.5 1.0
CE1 C:HIS151 4.2 16.2 1.0
CG C:HIS153 4.3 13.0 1.0
NE2 A:HIS231 4.3 20.2 1.0
CD2 A:HIS231 4.4 18.4 1.0
O C:HOH632 4.7 38.0 1.0
CE1 A:HIS231 4.9 20.3 1.0
NE2 C:HIS151 4.9 16.2 1.0
ND1 C:HIS151 5.0 17.2 1.0

Copper binding site 8 out of 9 in 4wtq

Go back to Copper Binding Sites List in 4wtq
Copper binding site 8 out of 9 in the Structure of the SSL1 Laccase Mutant M295L


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 8 of Structure of the SSL1 Laccase Mutant M295L within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Cu402

b:21.3
occ:1.00
 ND1 C:HIS290 2.0 20.6 1.0
ND1 C:HIS228 2.2 16.6 1.0
SG C:CYS285 2.2 18.8 1.0
CG C:HIS290 2.9 18.5 1.0
CE1 C:HIS290 3.0 20.6 1.0
CG C:HIS228 3.1 15.5 1.0
CE1 C:HIS228 3.1 17.9 1.0
CB C:CYS285 3.2 14.9 1.0
CB C:HIS290 3.3 19.6 1.0
CB C:HIS228 3.3 16.1 1.0
CA C:HIS228 3.7 15.2 1.0
CD1 C:LEU295 3.9 38.2 1.0
NE2 C:HIS290 4.0 23.5 1.0
CD2 C:HIS290 4.1 23.6 1.0
CG2 C:VAL287 4.2 17.5 1.0
O C:TYR227 4.2 17.3 1.0
CB C:VAL287 4.2 16.7 1.0
NE2 C:HIS228 4.2 18.2 1.0
CD2 C:HIS228 4.2 15.9 1.0
CA C:CYS285 4.5 15.6 1.0
N C:THR229 4.5 15.5 1.0
C C:HIS228 4.7 16.2 1.0
N C:HIS228 4.8 15.9 1.0
CA C:HIS290 4.8 19.6 1.0
C C:TYR227 4.9 15.0 1.0
CD2 C:PHE192 5.0 26.8 1.0

Copper binding site 9 out of 9 in 4wtq

Go back to Copper Binding Sites List in 4wtq
Copper binding site 9 out of 9 in the Structure of the SSL1 Laccase Mutant M295L


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 9 of Structure of the SSL1 Laccase Mutant M295L within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Cu403

b:22.9
occ:0.65
 NE2 C:HIS233 2.0 22.2 1.0
O B:O404 2.0 31.2 1.0
NE2 C:HIS284 2.1 21.7 1.0
NE2 B:HIS155 2.3 23.0 1.0
CE1 C:HIS233 2.7 24.8 1.0
CD2 C:HIS284 3.0 22.4 1.0
CE1 C:HIS284 3.1 22.5 1.0
CD2 C:HIS233 3.2 21.3 1.0
CE1 B:HIS155 3.2 25.7 1.0
CD2 B:HIS155 3.3 26.2 1.0
O B:HOH591 3.9 42.5 1.0
ND1 C:HIS233 3.9 24.0 1.0
CD2 C:HIS231 4.0 19.0 1.0
CE C:MET282 4.1 31.1 1.0
ND1 C:HIS284 4.1 21.1 1.0
CG C:HIS233 4.2 19.6 1.0
CG C:HIS284 4.2 20.5 1.0
NE2 B:HIS99 4.2 30.2 1.0
NE2 C:HIS231 4.3 20.7 1.0
ND1 B:HIS155 4.3 22.3 1.0
CD2 B:HIS161 4.4 27.0 1.0
CG B:HIS155 4.4 23.9 1.0
CE1 B:HIS99 4.6 27.7 1.0
CD2 B:HIS99 4.6 27.6 1.0
SD C:MET282 4.8 50.1 1.0
NE2 B:HIS161 5.0 30.6 1.0

Reference:

M.Gunne, A.Hoeppner, V.B.Urlacher. Structure of the SSL1 Laccase Mutant M295L To Be Published.
Page generated: Wed Jul 31 03:32:10 2024

Last articles

Zn in 9JYW
Zn in 9IR4
Zn in 9IR3
Zn in 9GMX
Zn in 9GMW
Zn in 9JEJ
Zn in 9ERF
Zn in 9ERE
Zn in 9EGV
Zn in 9EGW
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy