Copper in PDB 4w1t: Structure of the SSL1 Laccase Mutant H99Y with Depleted Type-2 Copper Ion

The structure of Structure of the SSL1 Laccase Mutant H99Y with Depleted Type-2 Copper Ion, PDB code: 4w1t was solved by M.Gunne, A.Hoeppner, V.D.Jaeger, V.B.Urlacher, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	52.28 / 1.55
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	51.490, 104.558, 163.408, 90.00, 90.00, 90.00
R / Rfree (%)	14.9 / 18

The binding sites of Copper atom in the Structure of the SSL1 Laccase Mutant H99Y with Depleted Type-2 Copper Ion (pdb code 4w1t). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 9 binding sites of Copper where determined in the Structure of the SSL1 Laccase Mutant H99Y with Depleted Type-2 Copper Ion, PDB code: 4w1t:
Jump to Copper binding site number: 1; 2; 3; 4; 5; 6; 7; 8; 9;

Copper binding site 1 out of 9 in the Structure of the SSL1 Laccase Mutant H99Y with Depleted Type-2 Copper Ion


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of Structure of the SSL1 Laccase Mutant H99Y with Depleted Type-2 Copper Ion within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu402 b:6.5 occ:0.90 ND1 A:HIS228 2.0 7.1 1.0 ND1 A:HIS290 2.0 8.5 1.0 SG A:CYS285 2.2 6.7 1.0 CE1 A:HIS290 3.0 7.8 1.0 CE1 A:HIS228 3.0 7.8 1.0 CG A:HIS228 3.0 6.6 1.0 CG A:HIS290 3.0 7.8 1.0 CB A:CYS285 3.1 6.8 1.0 CB A:HIS290 3.3 7.1 1.0 CB A:HIS228 3.4 6.1 1.0 SD A:MET295 3.4 11.3 1.0 CA A:HIS228 3.8 5.5 1.0 CB A:VAL287 4.1 7.1 1.0 NE2 A:HIS290 4.1 8.3 1.0 CG2 A:VAL287 4.1 7.1 1.0 NE2 A:HIS228 4.1 7.5 1.0 O A:TYR227 4.1 6.6 1.0 CD2 A:HIS290 4.1 8.1 1.0 CD2 A:HIS228 4.1 7.2 1.0 CE A:MET295 4.2 10.2 1.0 CA A:CYS285 4.5 6.3 1.0 N A:THR229 4.6 6.1 1.0 N A:HIS228 4.7 6.1 1.0 C A:HIS228 4.8 5.9 1.0 CA A:HIS290 4.8 7.1 1.0 C A:TYR227 4.8 5.7 1.0 CG A:MET295 4.9 10.2 1.0 CD2 A:PHE192 4.9 7.9 1.0 CG1 A:VAL287 4.9 7.1 1.0 N A:VAL287 5.0 5.7 1.0

Copper binding site 2 out of 9 in the Structure of the SSL1 Laccase Mutant H99Y with Depleted Type-2 Copper Ion


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of Structure of the SSL1 Laccase Mutant H99Y with Depleted Type-2 Copper Ion within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu403 b:9.2 occ:0.90 NE2 A:HIS284 2.1 7.9 1.0 NE2 C:HIS155 2.1 6.5 1.0 NE2 A:HIS233 2.1 8.8 1.0 O A:HOH630 2.2 11.1 1.0 O C:HOH772 2.4 30.2 1.0 CE1 A:HIS284 2.9 8.6 1.0 CE1 A:HIS233 2.9 8.6 1.0 CE1 C:HIS155 3.0 7.2 1.0 CD2 C:HIS155 3.1 6.7 1.0 CD2 A:HIS284 3.1 8.7 1.0 CD2 A:HIS233 3.2 8.7 1.0 O C:HOH729 3.6 22.7 1.0 ND1 A:HIS284 4.1 8.5 1.0 ND1 A:HIS233 4.1 8.2 1.0 ND1 C:HIS155 4.1 7.2 1.0 CD2 A:HIS231 4.2 8.0 1.0 CG C:HIS155 4.2 6.5 1.0 CG A:HIS284 4.2 7.7 1.0 CG A:HIS233 4.2 8.3 1.0 ND1 C:HIS161 4.4 16.1 1.0 O C:HOH770 4.4 28.5 1.0 NE2 A:HIS231 4.5 7.8 1.0 CE A:MET282 4.6 15.4 1.0 NE2 A:HIS286 4.6 7.6 1.0 CZ C:TYR99 4.7 8.3 1.0 CE2 C:TYR99 4.7 7.2 1.0 SD A:MET282 4.8 24.6 1.0 OH C:TYR99 4.9 9.2 1.0 CE1 C:HIS161 4.9 16.2 1.0

Copper binding site 3 out of 9 in the Structure of the SSL1 Laccase Mutant H99Y with Depleted Type-2 Copper Ion


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 3 of Structure of the SSL1 Laccase Mutant H99Y with Depleted Type-2 Copper Ion within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu404 b:10.2 occ:0.40 NE2 A:HIS153 1.9 12.7 1.0 NE2 A:HIS101 1.9 9.4 1.0 NE2 B:HIS286 2.2 12.5 1.0 CE1 A:HIS153 2.7 14.2 1.0 CE1 A:HIS101 2.8 10.8 1.0 CE1 B:HIS286 2.9 11.6 1.0 CD2 A:HIS153 3.0 10.9 1.0 CD2 A:HIS101 3.1 9.8 1.0 CD2 B:HIS286 3.1 9.6 1.0 O B:HOH599 3.4 16.6 1.0 CB A:ALA263 3.7 7.3 1.0 CE2 A:TYR99 3.8 13.3 1.0 ND1 A:HIS153 3.8 11.1 1.0 ND1 A:HIS101 3.9 10.1 1.0 ND1 B:HIS286 4.0 10.2 1.0 CE1 A:HIS151 4.0 9.5 1.0 CD2 A:TYR99 4.0 13.4 1.0 CG A:HIS153 4.0 11.4 1.0 CG B:HIS286 4.1 9.6 1.0 CG A:HIS101 4.1 9.8 1.0 O A:HOH709 4.6 34.1 1.0 NE2 B:HIS231 4.6 11.6 1.0 NE2 A:HIS151 4.7 9.7 1.0 CE1 A:HIS223 4.8 9.1 1.0 ND1 A:HIS151 4.9 10.1 1.0 CD2 B:HIS231 4.9 11.2 1.0 CA A:ALA263 4.9 7.9 1.0

Copper binding site 4 out of 9 in the Structure of the SSL1 Laccase Mutant H99Y with Depleted Type-2 Copper Ion


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 4 of Structure of the SSL1 Laccase Mutant H99Y with Depleted Type-2 Copper Ion within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu405 b:15.9 occ:0.85 NE2 B:HIS233 2.1 15.2 1.0 O B:HOH599 2.1 16.6 1.0 NE2 B:HIS284 2.1 15.7 1.0 NE2 A:HIS155 2.1 12.9 1.0 O A:HOH710 2.8 33.3 1.0 CE1 B:HIS233 2.9 17.5 1.0 CE1 B:HIS284 3.0 14.8 1.0 CE1 A:HIS155 3.1 13.2 1.0 CD2 A:HIS155 3.1 13.3 1.0 CD2 B:HIS284 3.2 12.8 1.0 CD2 B:HIS233 3.2 17.2 1.0 ND1 B:HIS233 4.1 16.1 1.0 ND1 B:HIS284 4.1 12.8 1.0 O A:HOH709 4.2 34.1 1.0 ND1 A:HIS155 4.2 13.1 1.0 CD2 B:HIS231 4.2 11.2 1.0 ND1 A:HIS161 4.2 18.7 1.0 CG B:HIS233 4.2 15.5 1.0 CG A:HIS155 4.3 13.2 1.0 CG B:HIS284 4.3 11.4 1.0 CE B:MET282 4.4 25.2 1.0 NE2 B:HIS231 4.6 11.6 1.0 NE2 B:HIS286 4.6 12.5 1.0 CE2 A:TYR99 4.6 13.3 1.0 CE1 A:HIS161 4.6 19.8 1.0 CZ A:TYR99 4.7 15.4 1.0 OH A:TYR99 4.8 15.1 1.0 CD2 B:HIS286 5.0 9.6 1.0

Copper binding site 5 out of 9 in the Structure of the SSL1 Laccase Mutant H99Y with Depleted Type-2 Copper Ion


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 5 of Structure of the SSL1 Laccase Mutant H99Y with Depleted Type-2 Copper Ion within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu406 b:3.9 occ:0.30 NE2 C:HIS101 1.9 6.1 1.0 NE2 C:HIS153 1.9 6.8 1.0 NE2 A:HIS286 2.2 7.6 1.0 CE1 C:HIS153 2.7 6.8 1.0 CE1 C:HIS101 2.7 6.2 1.0 CE1 A:HIS286 2.9 7.3 1.0 CD2 C:HIS101 3.0 6.1 1.0 CD2 C:HIS153 3.1 6.1 1.0 CD2 A:HIS286 3.1 6.8 1.0 O A:HOH630 3.3 11.1 1.0 CB C:ALA263 3.7 5.8 1.0 CE2 C:TYR99 3.8 7.2 1.0 ND1 C:HIS153 3.9 6.5 1.0 ND1 C:HIS101 3.9 6.3 1.0 CE1 C:HIS151 4.0 5.9 1.0 ND1 A:HIS286 4.0 6.0 1.0 CD2 C:TYR99 4.0 6.9 1.0 CG C:HIS153 4.1 5.5 1.0 CG C:HIS101 4.1 5.7 1.0 CG A:HIS286 4.1 5.8 1.0 O C:HOH770 4.6 28.5 1.0 NE2 A:HIS231 4.6 7.8 1.0 NE2 C:HIS151 4.7 5.3 1.0 CE1 C:HIS223 4.8 7.2 1.0 ND1 C:HIS151 4.8 6.2 1.0 O C:HOH729 4.9 22.7 1.0 CD2 A:HIS231 4.9 8.0 1.0 CA C:ALA263 4.9 5.3 1.0 CE1 A:HIS231 5.0 7.9 1.0

Copper binding site 6 out of 9 in the Structure of the SSL1 Laccase Mutant H99Y with Depleted Type-2 Copper Ion


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 6 of Structure of the SSL1 Laccase Mutant H99Y with Depleted Type-2 Copper Ion within 5.0Å range: probe atom residue distance (Å) B Occ B:Cu404 b:10.3 occ:0.95 ND1 B:HIS290 2.0 9.8 1.0 ND1 B:HIS228 2.0 9.9 1.0 SG B:CYS285 2.2 10.4 1.0 CE1 B:HIS290 2.9 11.0 1.0 CE1 B:HIS228 3.0 7.7 1.0 CG B:HIS290 3.0 11.4 1.0 CG B:HIS228 3.1 9.0 1.0 CB B:CYS285 3.2 9.2 1.0 CB B:HIS290 3.3 11.3 1.0 CB B:HIS228 3.4 8.2 1.0 SD B:MET295 3.4 14.6 1.0 CA B:HIS228 3.7 8.3 1.0 NE2 B:HIS290 4.1 12.3 1.0 CB B:VAL287 4.1 8.8 1.0 CG2 B:VAL287 4.1 9.3 1.0 CD2 B:HIS290 4.1 11.6 1.0 O B:TYR227 4.1 8.0 1.0 NE2 B:HIS228 4.1 10.1 1.0 CE B:MET295 4.1 15.1 1.0 CD2 B:HIS228 4.2 9.0 1.0 CA B:CYS285 4.5 9.6 1.0 N B:THR229 4.6 8.0 1.0 C B:HIS228 4.7 9.1 1.0 N B:HIS228 4.7 8.6 1.0 CA B:HIS290 4.8 11.4 1.0 C B:TYR227 4.8 7.6 1.0 CD2 B:PHE192 4.9 9.7 1.0 CG1 B:VAL287 4.9 9.6 1.0 CG B:MET295 5.0 15.2 1.0 CE2 B:PHE192 5.0 10.9 1.0 N B:VAL287 5.0 8.2 1.0

Copper binding site 7 out of 9 in the Structure of the SSL1 Laccase Mutant H99Y with Depleted Type-2 Copper Ion


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 7 of Structure of the SSL1 Laccase Mutant H99Y with Depleted Type-2 Copper Ion within 5.0Å range: probe atom residue distance (Å) B Occ B:Cu405 b:6.3 occ:0.30 NE2 B:HIS153 1.9 10.4 1.0 NE2 B:HIS101 2.0 10.5 1.0 NE2 C:HIS286 2.1 8.9 1.0 CE1 B:HIS153 2.6 11.9 1.0 CE1 B:HIS101 2.8 9.5 1.0 CE1 C:HIS286 2.9 9.0 1.0 CD2 B:HIS153 3.1 10.6 1.0 CD2 B:HIS101 3.1 8.9 1.0 CD2 C:HIS286 3.1 8.8 1.0 O C:HOH621 3.2 14.2 1.0 CB B:ALA263 3.8 7.1 1.0 CE2 B:TYR99 3.8 11.2 1.0 ND1 B:HIS153 3.9 11.7 1.0 ND1 C:HIS286 4.0 9.2 1.0 CD2 B:TYR99 4.0 10.8 1.0 ND1 B:HIS101 4.0 9.0 1.0 CG B:HIS153 4.1 10.6 1.0 CG C:HIS286 4.1 7.0 1.0 CE1 B:HIS151 4.1 10.6 1.0 CG B:HIS101 4.1 8.9 1.0 NE2 C:HIS231 4.6 9.4 1.0 O B:HOH662 4.7 28.9 1.0 NE2 B:HIS151 4.7 9.8 1.0 CD2 C:HIS231 4.8 8.8 1.0 CE1 B:HIS223 4.8 10.9 1.0 ND1 B:HIS151 4.9 10.1 1.0 CE1 C:HIS231 5.0 9.6 1.0 CA B:ALA263 5.0 6.2 1.0

Copper binding site 8 out of 9 in the Structure of the SSL1 Laccase Mutant H99Y with Depleted Type-2 Copper Ion


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 8 of Structure of the SSL1 Laccase Mutant H99Y with Depleted Type-2 Copper Ion within 5.0Å range: probe atom residue distance (Å) B Occ B:Cu406 b:11.4 occ:0.85 NE2 C:HIS233 2.0 13.9 1.0 NE2 C:HIS284 2.0 8.8 1.0 NE2 B:HIS155 2.1 9.1 1.0 O C:HOH621 2.2 14.2 1.0 O B:HOH606 2.9 27.2 1.0 CE1 C:HIS284 2.9 11.1 1.0 CE1 C:HIS233 2.9 11.1 1.0 CE1 B:HIS155 2.9 10.8 1.0 CD2 C:HIS284 3.1 10.5 1.0 CD2 C:HIS233 3.1 10.9 1.0 CD2 B:HIS155 3.1 10.9 1.0 ND1 C:HIS284 4.1 10.1 1.0 ND1 C:HIS233 4.1 11.0 1.0 ND1 B:HIS155 4.1 12.4 1.0 CG C:HIS284 4.2 8.9 1.0 CG B:HIS155 4.2 11.6 1.0 CD2 C:HIS231 4.2 8.8 1.0 CG C:HIS233 4.2 10.6 1.0 ND1 B:HIS161 4.3 14.8 1.0 O B:HOH662 4.4 28.9 1.0 CE C:MET282 4.4 19.9 1.0 NE2 C:HIS231 4.6 9.4 1.0 NE2 C:HIS286 4.6 8.9 1.0 CZ B:TYR99 4.7 10.7 1.0 CE2 B:TYR99 4.7 11.2 1.0 CE1 B:HIS161 4.8 14.2 1.0 SD C:MET282 4.8 26.1 1.0 OH B:TYR99 4.9 11.4 1.0

Copper binding site 9 out of 9 in the Structure of the SSL1 Laccase Mutant H99Y with Depleted Type-2 Copper Ion


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 9 of Structure of the SSL1 Laccase Mutant H99Y with Depleted Type-2 Copper Ion within 5.0Å range: probe atom residue distance (Å) B Occ C:Cu402 b:7.0 occ:0.90 ND1 C:HIS290 2.0 9.4 1.0 ND1 C:HIS228 2.0 5.7 1.0 SG C:CYS285 2.2 8.1 1.0 CE1 C:HIS228 3.0 6.8 1.0 CE1 C:HIS290 3.0 9.9 1.0 CG C:HIS290 3.0 8.3 1.0 CG C:HIS228 3.0 5.5 1.0 CB C:CYS285 3.2 7.2 1.0 CB C:HIS290 3.3 8.4 1.0 CB C:HIS228 3.4 5.3 1.0 SD C:MET295 3.4 10.7 1.0 CA C:HIS228 3.7 5.6 1.0 CG2 C:VAL287 4.0 7.7 1.0 CB C:VAL287 4.0 7.5 1.0 NE2 C:HIS228 4.1 6.7 1.0 NE2 C:HIS290 4.1 11.5 1.0 CD2 C:HIS228 4.1 7.0 1.0 CD2 C:HIS290 4.1 9.2 1.0 O C:TYR227 4.1 6.9 1.0 CE C:MET295 4.2 11.4 1.0 CA C:CYS285 4.5 6.8 1.0 N C:THR229 4.6 6.0 1.0 N C:HIS228 4.7 5.9 1.0 C C:HIS228 4.8 6.4 1.0 CA C:HIS290 4.8 8.6 1.0 C C:TYR227 4.9 6.8 1.0 CG C:MET295 4.9 10.6 1.0 CD2 C:PHE192 4.9 7.8 1.0 CG1 C:VAL287 4.9 8.8 1.0 CE2 C:PHE192 4.9 7.4 1.0 N C:VAL287 5.0 7.1 1.0

M.Gunne, A.Hoeppner, V.Deidre-Jaeger, V.B.Urlacher. Structure of the SSL1 Laccase Mutant H99Y with Depleted Type-2 Copper Ion To Be Published.