Copper in PDB 4uan: Structure of the SSL1 Laccase Mutant H99Q with Depleted Type-2 Copper Ion

The structure of Structure of the SSL1 Laccase Mutant H99Q with Depleted Type-2 Copper Ion, PDB code: 4uan was solved by M.Gunne, A.Hoeppner, V.D.Jaeger, V.B.Urlacher, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	52.14 / 1.80
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	51.522, 104.274, 163.086, 90.00, 90.00, 90.00
R / Rfree (%)	15.1 / 19.4

The binding sites of Copper atom in the Structure of the SSL1 Laccase Mutant H99Q with Depleted Type-2 Copper Ion (pdb code 4uan). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 9 binding sites of Copper where determined in the Structure of the SSL1 Laccase Mutant H99Q with Depleted Type-2 Copper Ion, PDB code: 4uan:
Jump to Copper binding site number: 1; 2; 3; 4; 5; 6; 7; 8; 9;

Copper binding site 1 out of 9 in the Structure of the SSL1 Laccase Mutant H99Q with Depleted Type-2 Copper Ion


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of Structure of the SSL1 Laccase Mutant H99Q with Depleted Type-2 Copper Ion within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu403 b:14.6 occ:0.70 O A:HOH687 1.8 25.8 1.0 NE2 C:HIS286 2.0 10.1 1.0 NE2 A:HIS101 2.1 9.6 1.0 NE2 A:HIS153 2.1 13.1 1.0 CE1 A:HIS153 2.8 13.7 1.0 CD2 C:HIS286 3.0 10.4 1.0 OE1 A:GLN99 3.0 42.3 1.0 CE1 A:HIS101 3.0 9.4 1.0 CE1 C:HIS286 3.0 10.4 1.0 CD2 A:HIS101 3.1 9.8 1.0 CD2 A:HIS153 3.3 10.6 1.0 CD A:GLN99 3.9 25.2 1.0 NE2 C:HIS231 4.0 9.8 1.0 ND1 A:HIS153 4.0 10.1 1.0 NE2 A:GLN99 4.1 25.7 1.0 ND1 A:HIS101 4.1 8.9 1.0 CG C:HIS286 4.1 10.1 1.0 ND1 C:HIS286 4.1 9.9 1.0 CG A:HIS101 4.2 8.9 1.0 CU A:CU407 4.3 17.4 0.7 CB A:ALA263 4.3 7.1 1.0 CG A:HIS153 4.3 9.9 1.0 CD2 C:HIS231 4.3 9.2 1.0 O A:HOH686 4.4 26.7 1.0 CE1 A:HIS151 4.5 9.0 1.0 CE1 C:HIS231 4.6 8.9 1.0 CG C:HIS231 5.0 9.6 1.0

Copper binding site 2 out of 9 in the Structure of the SSL1 Laccase Mutant H99Q with Depleted Type-2 Copper Ion


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of Structure of the SSL1 Laccase Mutant H99Q with Depleted Type-2 Copper Ion within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu404 b:12.0 occ:0.95 ND1 A:HIS290 2.0 13.2 1.0 ND1 A:HIS228 2.0 9.6 1.0 SG A:CYS285 2.1 12.1 1.0 CG A:HIS290 3.0 12.1 1.0 CG A:HIS228 3.0 9.2 1.0 CE1 A:HIS290 3.0 14.5 1.0 CE1 A:HIS228 3.0 10.2 1.0 CB A:CYS285 3.2 11.8 1.0 CB A:HIS290 3.3 12.8 1.0 CB A:HIS228 3.3 8.9 1.0 SD A:MET295 3.4 14.1 1.0 CA A:HIS228 3.7 8.9 1.0 CB A:VAL287 4.1 10.3 1.0 CG2 A:VAL287 4.1 9.1 1.0 NE2 A:HIS290 4.1 16.0 1.0 CD2 A:HIS290 4.1 14.0 1.0 O A:TYR227 4.1 11.2 1.0 NE2 A:HIS228 4.1 9.7 1.0 CD2 A:HIS228 4.1 9.4 1.0 CE A:MET295 4.2 13.5 1.0 CA A:CYS285 4.5 11.3 1.0 N A:THR229 4.6 9.5 1.0 C A:HIS228 4.7 9.7 1.0 N A:HIS228 4.8 9.7 1.0 CA A:HIS290 4.9 12.6 1.0 C A:TYR227 4.9 9.4 1.0 CD2 A:PHE192 4.9 11.6 1.0 CG1 A:VAL287 4.9 10.0 1.0 CG A:MET295 4.9 14.0 1.0 N A:VAL287 5.0 12.1 1.0 CE2 A:PHE192 5.0 11.0 1.0

Copper binding site 3 out of 9 in the Structure of the SSL1 Laccase Mutant H99Q with Depleted Type-2 Copper Ion


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 3 of Structure of the SSL1 Laccase Mutant H99Q with Depleted Type-2 Copper Ion within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu405 b:21.6 occ:0.75 NE2 A:HIS233 2.1 16.8 1.0 NE2 A:HIS284 2.3 18.4 1.0 O A:HOH643 2.3 32.4 1.0 NE2 B:HIS155 2.3 20.2 1.0 CE1 A:HIS233 2.8 15.5 1.0 CE1 A:HIS284 3.0 17.1 1.0 CD2 B:HIS155 3.1 22.1 1.0 CD2 A:HIS233 3.2 16.4 1.0 CE1 B:HIS155 3.4 20.3 1.0 CD2 A:HIS284 3.4 19.1 1.0 O B:HOH601 3.6 32.4 1.0 NE2 B:GLN99 3.8 33.0 1.0 CD2 A:HIS231 3.9 12.4 1.0 ND1 A:HIS233 4.0 16.8 1.0 CU A:CU406 4.2 17.5 0.8 NE2 A:HIS231 4.2 13.3 1.0 ND1 A:HIS284 4.2 16.2 1.0 CG A:HIS233 4.3 15.2 1.0 CG B:HIS155 4.3 20.3 1.0 ND1 B:HIS155 4.4 18.4 1.0 CG A:HIS284 4.4 16.6 1.0 NE2 A:HIS286 4.4 14.7 1.0 CD B:GLN99 4.5 30.7 1.0 SD A:MET282 4.7 36.8 1.0 CD2 A:HIS286 4.8 13.1 1.0 CD2 B:HIS161 4.9 20.0 1.0 CE A:MET282 4.9 26.0 1.0 CG B:GLN99 5.0 32.0 1.0

Copper binding site 4 out of 9 in the Structure of the SSL1 Laccase Mutant H99Q with Depleted Type-2 Copper Ion


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 4 of Structure of the SSL1 Laccase Mutant H99Q with Depleted Type-2 Copper Ion within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu406 b:17.5 occ:0.75 O A:HOH643 1.9 32.4 1.0 NE2 B:HIS101 2.1 14.4 1.0 NE2 A:HIS286 2.1 14.7 1.0 NE2 B:HIS153 2.1 15.7 1.0 CE1 B:HIS153 2.8 16.4 1.0 CE1 B:HIS101 2.9 13.1 1.0 CD2 A:HIS286 3.0 13.1 1.0 CE1 A:HIS286 3.1 13.4 1.0 CD2 B:HIS101 3.2 13.5 1.0 CD2 B:HIS153 3.2 15.5 1.0 NE2 B:GLN99 3.7 33.0 1.0 NE2 A:HIS231 4.0 13.3 1.0 ND1 B:HIS153 4.1 16.7 1.0 ND1 B:HIS101 4.1 12.6 1.0 CG B:GLN99 4.1 32.0 1.0 CG A:HIS286 4.2 12.1 1.0 CU A:CU405 4.2 21.6 0.8 CD2 A:HIS231 4.2 12.4 1.0 ND1 A:HIS286 4.2 12.5 1.0 CG B:HIS101 4.2 13.1 1.0 CG B:HIS153 4.3 14.5 1.0 O B:HOH601 4.3 32.4 1.0 CB B:ALA263 4.4 10.3 1.0 CD B:GLN99 4.4 30.7 1.0 CE1 B:HIS151 4.6 13.3 1.0 CE1 A:HIS231 4.6 13.2 1.0 CG A:HIS231 4.9 11.7 1.0

Copper binding site 5 out of 9 in the Structure of the SSL1 Laccase Mutant H99Q with Depleted Type-2 Copper Ion


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 5 of Structure of the SSL1 Laccase Mutant H99Q with Depleted Type-2 Copper Ion within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu407 b:17.4 occ:0.70 NE2 C:HIS233 2.1 12.5 1.0 NE2 C:HIS284 2.2 16.2 1.0 NE2 A:HIS155 2.2 13.3 1.0 O A:HOH687 2.5 25.8 1.0 CE1 C:HIS233 2.9 12.1 1.0 CE1 C:HIS284 3.0 16.6 1.0 CD2 A:HIS155 3.1 12.5 1.0 CD2 C:HIS233 3.2 11.9 1.0 CD2 C:HIS284 3.3 16.2 1.0 CE1 A:HIS155 3.3 14.1 1.0 O A:HOH686 3.7 26.7 1.0 CD2 C:HIS231 3.9 9.2 1.0 NE2 A:GLN99 4.0 25.7 1.0 ND1 C:HIS233 4.1 13.1 1.0 NE2 C:HIS231 4.1 9.8 1.0 ND1 C:HIS284 4.2 14.8 1.0 CU A:CU403 4.3 14.6 0.7 CG C:HIS233 4.3 10.5 1.0 CD A:GLN99 4.3 25.2 1.0 CG A:HIS155 4.3 11.9 1.0 CG C:HIS284 4.4 13.9 1.0 ND1 A:HIS155 4.4 12.3 1.0 SD C:MET282 4.4 28.2 1.0 OE1 A:GLN99 4.4 42.3 1.0 NE2 C:HIS286 4.4 10.1 1.0 CD2 C:HIS286 4.7 10.4 1.0

Copper binding site 6 out of 9 in the Structure of the SSL1 Laccase Mutant H99Q with Depleted Type-2 Copper Ion


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 6 of Structure of the SSL1 Laccase Mutant H99Q with Depleted Type-2 Copper Ion within 5.0Å range: probe atom residue distance (Å) B Occ B:Cu404 b:15.8 occ:1.00 ND1 B:HIS290 2.0 14.2 1.0 ND1 B:HIS228 2.0 13.3 1.0 SG B:CYS285 2.1 14.2 1.0 CG B:HIS290 2.9 16.0 1.0 CE1 B:HIS228 3.0 12.2 1.0 CE1 B:HIS290 3.0 15.8 1.0 CG B:HIS228 3.0 12.5 1.0 CB B:CYS285 3.2 15.2 1.0 CB B:HIS290 3.3 15.7 1.0 SD B:MET295 3.3 19.4 1.0 CB B:HIS228 3.4 12.0 1.0 CA B:HIS228 3.7 12.9 1.0 CB B:VAL287 4.1 12.3 1.0 CG2 B:VAL287 4.1 12.3 1.0 CD2 B:HIS290 4.1 15.8 1.0 NE2 B:HIS290 4.1 16.1 1.0 O B:TYR227 4.1 12.0 1.0 NE2 B:HIS228 4.1 13.0 1.0 CD2 B:HIS228 4.2 11.6 1.0 CE B:MET295 4.3 18.1 1.0 CA B:CYS285 4.5 15.6 1.0 N B:THR229 4.6 12.2 1.0 N B:HIS228 4.7 11.9 1.0 C B:HIS228 4.7 13.1 1.0 CA B:HIS290 4.8 15.7 1.0 C B:TYR227 4.8 11.1 1.0 CG B:MET295 4.8 20.4 1.0 CD2 B:PHE192 4.9 13.4 1.0 CG1 B:VAL287 4.9 12.1 1.0

Copper binding site 7 out of 9 in the Structure of the SSL1 Laccase Mutant H99Q with Depleted Type-2 Copper Ion


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 7 of Structure of the SSL1 Laccase Mutant H99Q with Depleted Type-2 Copper Ion within 5.0Å range: probe atom residue distance (Å) B Occ B:Cu405 b:29.2 occ:0.70 NE2 B:HIS233 2.1 21.1 1.0 NE2 C:HIS155 2.3 20.1 1.0 O C:HOH649 2.4 31.8 1.0 NE2 B:HIS284 2.5 29.0 1.0 CE1 B:HIS233 2.9 23.1 1.0 CD2 C:HIS155 3.2 19.7 1.0 CE1 B:HIS284 3.2 22.9 1.0 CD2 B:HIS233 3.3 21.2 1.0 CE1 C:HIS155 3.4 19.5 1.0 CD2 B:HIS284 3.6 23.1 1.0 NE2 C:GLN99 3.6 34.1 1.0 O C:HOH650 3.8 27.0 1.0 CD2 B:HIS231 3.9 15.4 1.0 ND1 B:HIS233 4.1 22.1 1.0 NE2 B:HIS231 4.2 16.7 1.0 CU B:CU406 4.2 17.6 0.7 CG B:HIS233 4.3 18.9 1.0 NE2 B:HIS286 4.4 18.4 1.0 CG C:HIS155 4.4 19.1 1.0 CD C:GLN99 4.4 33.9 1.0 ND1 B:HIS284 4.4 18.6 1.0 ND1 C:HIS155 4.4 20.6 1.0 CG B:HIS284 4.6 20.5 1.0 CD2 B:HIS286 4.7 15.5 1.0 SD B:MET282 4.7 44.1 1.0 CD2 C:HIS161 4.8 24.5 1.0 CG C:GLN99 4.9 33.2 1.0

Copper binding site 8 out of 9 in the Structure of the SSL1 Laccase Mutant H99Q with Depleted Type-2 Copper Ion


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 8 of Structure of the SSL1 Laccase Mutant H99Q with Depleted Type-2 Copper Ion within 5.0Å range: probe atom residue distance (Å) B Occ B:Cu406 b:17.6 occ:0.70 O C:HOH649 2.0 31.8 1.0 NE2 B:HIS286 2.0 18.4 1.0 NE2 C:HIS101 2.1 15.1 1.0 NE2 C:HIS153 2.1 16.7 1.0 CE1 C:HIS153 2.8 18.4 1.0 CD2 B:HIS286 3.0 15.5 1.0 CE1 C:HIS101 3.0 14.9 1.0 CE1 B:HIS286 3.1 17.0 1.0 CD2 C:HIS101 3.1 16.6 1.0 CD2 C:HIS153 3.2 16.1 1.0 NE2 C:GLN99 3.7 34.1 1.0 ND1 C:HIS153 4.0 15.2 1.0 CG B:HIS286 4.1 16.2 1.0 ND1 B:HIS286 4.1 14.6 1.0 ND1 C:HIS101 4.1 13.9 1.0 NE2 B:HIS231 4.2 16.7 1.0 CG C:HIS101 4.2 15.1 1.0 CG C:GLN99 4.2 33.2 1.0 CU B:CU405 4.2 29.2 0.7 CB C:ALA263 4.2 11.9 1.0 CG C:HIS153 4.3 16.5 1.0 O C:HOH650 4.3 27.0 1.0 CD2 B:HIS231 4.3 15.4 1.0 CE1 C:HIS151 4.4 13.9 1.0 CD C:GLN99 4.5 33.9 1.0 CE1 B:HIS231 4.7 14.6 1.0

Copper binding site 9 out of 9 in the Structure of the SSL1 Laccase Mutant H99Q with Depleted Type-2 Copper Ion


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 9 of Structure of the SSL1 Laccase Mutant H99Q with Depleted Type-2 Copper Ion within 5.0Å range: probe atom residue distance (Å) B Occ C:Cu402 b:11.4 occ:0.95 ND1 C:HIS228 2.0 8.4 1.0 ND1 C:HIS290 2.1 11.2 1.0 SG C:CYS285 2.2 10.3 1.0 CE1 C:HIS228 2.9 8.8 1.0 CG C:HIS290 3.0 12.2 1.0 CG C:HIS228 3.0 8.1 1.0 CE1 C:HIS290 3.1 12.3 1.0 CB C:CYS285 3.2 10.2 1.0 CB C:HIS290 3.3 11.0 1.0 SD C:MET295 3.3 14.7 1.0 CB C:HIS228 3.4 7.6 1.0 CA C:HIS228 3.7 7.9 1.0 CB C:VAL287 4.0 8.5 1.0 CE C:MET295 4.0 14.1 1.0 NE2 C:HIS228 4.1 8.9 1.0 CD2 C:HIS228 4.1 9.0 1.0 CD2 C:HIS290 4.1 12.9 1.0 CG2 C:VAL287 4.1 8.1 1.0 NE2 C:HIS290 4.1 11.9 1.0 O C:TYR227 4.1 9.6 1.0 CA C:CYS285 4.6 10.2 1.0 N C:THR229 4.6 8.4 1.0 N C:HIS228 4.7 8.0 1.0 C C:HIS228 4.7 9.1 1.0 CA C:HIS290 4.8 10.8 1.0 CG C:MET295 4.8 15.4 1.0 C C:TYR227 4.9 8.4 1.0 CG1 C:VAL287 4.9 8.8 1.0 CD2 C:PHE192 4.9 10.9 1.0 N C:VAL287 5.0 9.3 1.0

M.Gunne, A.Hoeppner, V.Deidre-Jaeger, V.B.Urlacher. Structure of the SSL1 Laccase Mutant H99Q with Depleted Type-2 Copper Ion To Be Published.