Copper in PDB 4uah: Structure of the SSL1 Laccase Mutant H99N with Depleted Type-2 Copper Ion

The structure of Structure of the SSL1 Laccase Mutant H99N with Depleted Type-2 Copper Ion, PDB code: 4uah was solved by M.Gunne, A.Hoeppner, V.D.Jaeger, V.B.Urlacher, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	54.55 / 1.73
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	51.586, 104.328, 163.637, 90.00, 90.00, 90.00
R / Rfree (%)	17 / 20.7

The binding sites of Copper atom in the Structure of the SSL1 Laccase Mutant H99N with Depleted Type-2 Copper Ion (pdb code 4uah). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 9 binding sites of Copper where determined in the Structure of the SSL1 Laccase Mutant H99N with Depleted Type-2 Copper Ion, PDB code: 4uah:
Jump to Copper binding site number: 1; 2; 3; 4; 5; 6; 7; 8; 9;

Copper binding site 1 out of 9 in the Structure of the SSL1 Laccase Mutant H99N with Depleted Type-2 Copper Ion


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of Structure of the SSL1 Laccase Mutant H99N with Depleted Type-2 Copper Ion within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu401 b:7.8 occ:0.85 ND1 A:HIS228 2.0 8.2 1.0 ND1 A:HIS290 2.0 9.4 1.0 SG A:CYS285 2.1 7.5 1.0 CE1 A:HIS228 3.0 8.8 1.0 CG A:HIS290 3.0 8.9 1.0 CG A:HIS228 3.0 7.5 1.0 CE1 A:HIS290 3.0 8.4 1.0 CB A:CYS285 3.1 7.7 1.0 CB A:HIS290 3.3 8.8 1.0 CB A:HIS228 3.4 7.0 1.0 SD A:MET295 3.5 12.8 1.0 CA A:HIS228 3.7 7.0 1.0 CB A:VAL287 4.0 5.9 1.0 O A:TYR227 4.1 7.8 1.0 NE2 A:HIS228 4.1 8.8 1.0 NE2 A:HIS290 4.1 9.7 1.0 CD2 A:HIS290 4.1 10.1 1.0 CG2 A:VAL287 4.1 5.5 1.0 CD2 A:HIS228 4.1 8.6 1.0 CE A:MET295 4.1 9.2 1.0 CA A:CYS285 4.5 7.3 1.0 N A:THR229 4.6 7.0 1.0 N A:HIS228 4.7 6.4 1.0 C A:HIS228 4.7 7.5 1.0 C A:TYR227 4.8 7.2 1.0 CA A:HIS290 4.8 8.4 1.0 N A:VAL287 4.9 7.6 1.0 CG1 A:VAL287 4.9 5.7 1.0 CG A:MET295 5.0 10.6 1.0

Copper binding site 2 out of 9 in the Structure of the SSL1 Laccase Mutant H99N with Depleted Type-2 Copper Ion


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of Structure of the SSL1 Laccase Mutant H99N with Depleted Type-2 Copper Ion within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu402 b:20.8 occ:0.75 NE2 A:HIS233 2.0 9.0 1.0 O A:HOH640 2.1 15.2 1.0 NE2 A:HIS284 2.2 8.7 1.0 NE2 C:HIS155 2.2 7.7 1.0 CE1 A:HIS233 2.8 8.4 1.0 CE1 A:HIS284 2.9 9.9 1.0 O C:HOH635 3.1 19.8 1.0 CD2 A:HIS233 3.1 9.2 1.0 CD2 C:HIS155 3.2 6.8 1.0 CE1 C:HIS155 3.2 7.8 1.0 CD2 A:HIS284 3.3 9.6 1.0 O C:HOH583 3.7 14.8 1.0 CD2 A:HIS231 3.9 7.5 1.0 ND1 A:HIS233 4.0 8.8 1.0 CU A:CU405 4.0 16.1 0.6 ND1 A:HIS284 4.1 9.8 1.0 NE2 A:HIS231 4.1 7.7 1.0 CG A:HIS233 4.2 8.8 1.0 ND1 C:HIS155 4.3 7.7 1.0 CG C:HIS155 4.3 6.9 1.0 CG A:HIS284 4.4 9.1 1.0 NE2 A:HIS286 4.4 6.4 1.0 CD2 A:HIS286 4.7 7.2 1.0 ND1 C:HIS161 4.8 16.7 1.0 SD A:MET282 4.8 26.7 1.0 OD1 C:ASN99 4.8 13.8 1.0 CE A:MET282 4.9 15.5 1.0

Copper binding site 3 out of 9 in the Structure of the SSL1 Laccase Mutant H99N with Depleted Type-2 Copper Ion


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 3 of Structure of the SSL1 Laccase Mutant H99N with Depleted Type-2 Copper Ion within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu403 b:22.9 occ:0.70 NE2 B:HIS286 2.1 14.1 1.0 NE2 A:HIS153 2.1 17.1 1.0 NE2 A:HIS101 2.1 13.3 1.0 O A:HOH641 2.7 24.8 1.0 O A:HOH639 2.7 31.4 1.0 CD2 B:HIS286 2.8 13.2 1.0 CE1 A:HIS153 2.8 16.1 1.0 CE1 A:HIS101 3.0 12.3 1.0 CD2 A:HIS101 3.2 15.2 1.0 CE1 B:HIS286 3.2 15.3 1.0 CD2 A:HIS153 3.2 14.5 1.0 OD1 A:ASN99 3.9 20.0 1.0 NE2 B:HIS231 4.0 14.9 1.0 ND1 A:HIS153 4.1 15.1 1.0 CG B:HIS286 4.1 11.3 1.0 ND1 A:HIS101 4.1 13.1 1.0 ND1 B:HIS286 4.2 11.5 1.0 CG A:HIS101 4.2 11.9 1.0 CD2 B:HIS231 4.2 14.2 1.0 CG A:HIS153 4.3 14.5 1.0 CB A:ALA263 4.3 9.5 1.0 CU A:CU404 4.3 30.2 0.7 O A:HOH598 4.4 20.7 1.0 CE1 A:HIS151 4.6 13.2 1.0 CE1 B:HIS231 4.6 14.0 1.0 CG B:HIS231 5.0 12.2 1.0 CG A:ASN99 5.0 22.8 1.0

Copper binding site 4 out of 9 in the Structure of the SSL1 Laccase Mutant H99N with Depleted Type-2 Copper Ion


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 4 of Structure of the SSL1 Laccase Mutant H99N with Depleted Type-2 Copper Ion within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu404 b:30.2 occ:0.70 O A:HOH641 1.8 24.8 1.0 NE2 B:HIS233 2.0 16.4 1.0 NE2 B:HIS284 2.3 17.7 1.0 NE2 A:HIS155 2.3 13.1 1.0 CE1 B:HIS233 2.7 15.8 1.0 CE1 B:HIS284 3.1 17.6 1.0 CE1 A:HIS155 3.2 13.1 1.0 CD2 B:HIS233 3.3 14.8 1.0 O A:HOH639 3.3 31.4 1.0 CD2 B:HIS284 3.3 15.9 1.0 CD2 A:HIS155 3.4 13.2 1.0 O A:HOH598 3.8 20.7 1.0 ND1 B:HIS233 3.9 15.8 1.0 CD2 B:HIS231 4.0 14.2 1.0 CG B:HIS233 4.2 14.5 1.0 ND1 B:HIS284 4.2 15.1 1.0 NE2 B:HIS231 4.3 14.9 1.0 ND1 A:HIS155 4.3 13.6 1.0 CU A:CU403 4.3 22.9 0.7 CG B:HIS284 4.4 15.1 1.0 CG A:HIS155 4.5 13.6 1.0 CD2 A:HIS161 4.5 23.4 1.0 CE B:MET282 4.5 22.2 1.0 NE2 B:HIS286 4.6 14.1 1.0 CD2 B:HIS286 4.7 13.2 1.0 SD B:MET282 4.9 33.7 1.0 OD1 A:ASN99 4.9 20.0 1.0

Copper binding site 5 out of 9 in the Structure of the SSL1 Laccase Mutant H99N with Depleted Type-2 Copper Ion


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 5 of Structure of the SSL1 Laccase Mutant H99N with Depleted Type-2 Copper Ion within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu405 b:16.1 occ:0.60 NE2 A:HIS286 2.0 6.4 1.0 NE2 C:HIS101 2.1 8.2 1.0 NE2 C:HIS153 2.2 8.8 1.0 O A:HOH640 2.3 15.2 1.0 O C:HOH635 2.5 19.8 1.0 CE1 C:HIS153 2.9 9.7 1.0 CD2 A:HIS286 2.9 7.2 1.0 CE1 C:HIS101 2.9 8.3 1.0 CE1 A:HIS286 3.0 6.6 1.0 CD2 C:HIS101 3.2 7.9 1.0 CD2 C:HIS153 3.5 7.7 1.0 OD1 C:ASN99 3.8 13.8 1.0 NE2 A:HIS231 3.8 7.7 1.0 CU A:CU402 4.0 20.8 0.8 CD2 A:HIS231 4.1 7.5 1.0 CG A:HIS286 4.1 6.5 1.0 ND1 C:HIS101 4.1 7.3 1.0 ND1 A:HIS286 4.1 5.6 1.0 ND1 C:HIS153 4.1 7.4 1.0 CG C:HIS101 4.2 6.8 1.0 CG C:HIS153 4.4 7.2 1.0 CE1 A:HIS231 4.5 7.6 1.0 O C:HOH583 4.5 14.8 1.0 CB C:ALA263 4.5 6.3 1.0 CG A:HIS231 4.8 8.0 1.0 CE1 C:HIS151 4.8 7.3 1.0 CG C:ASN99 4.8 11.5 1.0 CD2 C:HIS155 4.8 6.8 1.0 CE1 A:HIS284 4.9 9.9 1.0 NE2 C:HIS155 5.0 7.7 1.0

Copper binding site 6 out of 9 in the Structure of the SSL1 Laccase Mutant H99N with Depleted Type-2 Copper Ion


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 6 of Structure of the SSL1 Laccase Mutant H99N with Depleted Type-2 Copper Ion within 5.0Å range: probe atom residue distance (Å) B Occ B:Cu403 b:12.3 occ:0.95 ND1 B:HIS290 2.0 13.0 1.0 ND1 B:HIS228 2.0 10.8 1.0 SG B:CYS285 2.2 11.8 1.0 CG B:HIS290 2.9 13.8 1.0 CE1 B:HIS290 3.0 14.2 1.0 CE1 B:HIS228 3.0 11.5 1.0 CG B:HIS228 3.1 11.1 1.0 CB B:CYS285 3.1 12.2 1.0 CB B:HIS290 3.2 14.2 1.0 CB B:HIS228 3.4 10.3 1.0 SD B:MET295 3.5 17.4 1.0 CA B:HIS228 3.7 10.7 1.0 CB B:VAL287 4.0 8.5 1.0 CG2 B:VAL287 4.0 9.8 1.0 O B:TYR227 4.1 9.4 1.0 NE2 B:HIS290 4.1 14.6 1.0 CD2 B:HIS290 4.1 14.0 1.0 NE2 B:HIS228 4.1 11.4 1.0 CD2 B:HIS228 4.2 10.1 1.0 CE B:MET295 4.3 17.9 1.0 CA B:CYS285 4.5 12.5 1.0 N B:THR229 4.6 9.8 1.0 N B:HIS228 4.7 10.2 1.0 C B:HIS228 4.7 10.7 1.0 CA B:HIS290 4.8 13.8 1.0 C B:TYR227 4.8 9.5 1.0 CG1 B:VAL287 4.8 9.7 1.0 N B:VAL287 4.9 9.0 1.0 CD2 B:PHE192 5.0 11.5 1.0

Copper binding site 7 out of 9 in the Structure of the SSL1 Laccase Mutant H99N with Depleted Type-2 Copper Ion


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 7 of Structure of the SSL1 Laccase Mutant H99N with Depleted Type-2 Copper Ion within 5.0Å range: probe atom residue distance (Å) B Occ B:Cu404 b:19.8 occ:0.70 NE2 B:HIS101 2.0 11.5 1.0 NE2 C:HIS286 2.0 13.2 1.0 NE2 B:HIS153 2.2 12.6 1.0 O B:HOH601 2.2 22.9 1.0 O C:HOH649 2.3 24.5 1.0 CE1 B:HIS101 2.9 11.2 1.0 CE1 B:HIS153 2.9 16.5 1.0 CD2 C:HIS286 3.0 12.9 1.0 CD2 B:HIS101 3.1 11.5 1.0 CE1 C:HIS286 3.1 13.0 1.0 CD2 B:HIS153 3.3 15.0 1.0 OD1 B:ASN99 3.7 13.4 1.0 NE2 C:HIS231 3.8 9.2 1.0 ND1 B:HIS101 4.0 12.1 1.0 CD2 C:HIS231 4.0 9.1 1.0 CG C:HIS286 4.1 11.1 1.0 CG B:HIS101 4.1 11.5 1.0 ND1 B:HIS153 4.1 15.2 1.0 ND1 C:HIS286 4.2 11.9 1.0 CU B:CU405 4.2 20.7 0.7 CG B:HIS153 4.3 14.6 1.0 O B:HOH553 4.4 21.7 1.0 CE1 C:HIS231 4.5 9.7 1.0 CB B:ALA263 4.5 8.2 1.0 CE1 B:HIS151 4.6 12.0 1.0 CG C:HIS231 4.8 8.9 1.0 CG B:ASN99 4.9 12.7 1.0 CE1 C:HIS284 4.9 12.9 1.0 CD2 B:HIS155 5.0 15.8 1.0

Copper binding site 8 out of 9 in the Structure of the SSL1 Laccase Mutant H99N with Depleted Type-2 Copper Ion


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 8 of Structure of the SSL1 Laccase Mutant H99N with Depleted Type-2 Copper Ion within 5.0Å range: probe atom residue distance (Å) B Occ B:Cu405 b:20.7 occ:0.65 NE2 C:HIS233 2.0 13.8 1.0 O C:HOH649 2.1 24.5 1.0 NE2 B:HIS155 2.2 13.0 1.0 NE2 C:HIS284 2.2 11.8 1.0 CE1 C:HIS233 2.8 14.8 1.0 CE1 C:HIS284 2.9 12.9 1.0 CE1 B:HIS155 3.1 14.0 1.0 CD2 C:HIS233 3.2 12.9 1.0 CD2 B:HIS155 3.2 15.8 1.0 O B:HOH601 3.3 22.9 1.0 CD2 C:HIS284 3.4 13.9 1.0 O B:HOH553 3.6 21.7 1.0 CD2 C:HIS231 4.0 9.1 1.0 ND1 C:HIS233 4.0 13.3 1.0 ND1 C:HIS284 4.1 12.9 1.0 CG C:HIS233 4.2 12.9 1.0 ND1 B:HIS155 4.2 13.9 1.0 CU B:CU404 4.2 19.8 0.7 NE2 C:HIS231 4.2 9.2 1.0 CG B:HIS155 4.3 13.6 1.0 CG C:HIS284 4.4 12.6 1.0 ND1 B:HIS161 4.5 20.5 1.0 CE C:MET282 4.5 18.5 1.0 NE2 C:HIS286 4.6 13.2 1.0 OD1 B:ASN99 4.7 13.4 1.0 CD2 C:HIS286 4.9 12.9 1.0 SD C:MET282 4.9 31.2 1.0

Copper binding site 9 out of 9 in the Structure of the SSL1 Laccase Mutant H99N with Depleted Type-2 Copper Ion


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 9 of Structure of the SSL1 Laccase Mutant H99N with Depleted Type-2 Copper Ion within 5.0Å range: probe atom residue distance (Å) B Occ C:Cu402 b:8.8 occ:0.90 ND1 C:HIS290 2.0 12.0 1.0 ND1 C:HIS228 2.0 6.6 1.0 SG C:CYS285 2.1 9.5 1.0 CG C:HIS290 2.9 9.9 1.0 CE1 C:HIS290 3.0 12.0 1.0 CE1 C:HIS228 3.0 7.2 1.0 CG C:HIS228 3.0 6.7 1.0 CB C:CYS285 3.1 8.8 1.0 CB C:HIS290 3.2 10.2 1.0 CB C:HIS228 3.4 6.3 1.0 SD C:MET295 3.5 12.4 1.0 CA C:HIS228 3.7 6.3 1.0 O C:TYR227 4.0 8.8 1.0 CD2 C:HIS290 4.1 12.3 1.0 NE2 C:HIS290 4.1 12.7 1.0 CB C:VAL287 4.1 8.2 1.0 CG2 C:VAL287 4.1 7.5 1.0 NE2 C:HIS228 4.1 7.4 1.0 CD2 C:HIS228 4.1 6.7 1.0 CE C:MET295 4.3 11.1 1.0 N C:THR229 4.5 6.7 1.0 CA C:CYS285 4.5 9.4 1.0 C C:HIS228 4.6 6.8 1.0 N C:HIS228 4.8 6.7 1.0 CA C:HIS290 4.8 9.7 1.0 C C:TYR227 4.8 7.2 1.0 CD2 C:PHE192 4.9 9.1 1.0 CG1 C:VAL287 4.9 8.7 1.0 N C:VAL287 4.9 8.8 1.0 CG C:MET295 5.0 12.8 1.0

M.Gunne, A.Hoeppner, V.Deidre-Jaeger, V.B.Urlacher. Structure of the SSL1 Laccase Mutant H99N with Depleted Type-2 Copper Ion To Be Published.