Copper in PDB 4jhv: T2-Depleted Laccase From Coriolopsis Caperata

The structure of T2-Depleted Laccase From Coriolopsis Caperata, PDB code: 4jhv was solved by K.M.Polyakov, T.V.Fedorova, O.A.Glazunova, S.A.Kurzeev, L.G.Maloshenok, V.S.Lamzin, O.V.Koroleva, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.79 / 1.60
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	62.780, 84.910, 116.950, 90.00, 90.00, 90.00
R / Rfree (%)	15.6 / 17.8

The binding sites of Copper atom in the T2-Depleted Laccase From Coriolopsis Caperata (pdb code 4jhv). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 4 binding sites of Copper where determined in the T2-Depleted Laccase From Coriolopsis Caperata, PDB code: 4jhv:
Jump to Copper binding site number: 1; 2; 3; 4;

Copper binding site 1 out of 4 in the T2-Depleted Laccase From Coriolopsis Caperata


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of T2-Depleted Laccase From Coriolopsis Caperata within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu501 b:10.7 occ:0.80 NE2 A:HIS449 2.0 13.0 1.0 NE2 A:HIS399 2.0 10.6 1.0 NE2 A:HIS111 2.0 11.1 1.0 O A:HOH612 2.4 9.1 0.5 CE1 A:HIS399 2.8 10.7 1.0 CE1 A:HIS449 2.9 13.6 1.0 CE1 A:HIS111 2.9 11.5 1.0 CD2 A:HIS449 3.0 12.2 1.0 CD2 A:HIS111 3.1 11.1 1.0 CD2 A:HIS399 3.1 9.7 1.0 CD2 A:HIS397 3.9 13.3 1.0 ND1 A:HIS399 4.0 10.2 1.0 CD2 A:PHE447 4.0 12.6 1.0 ND1 A:HIS449 4.1 12.0 1.0 CG A:HIS449 4.1 11.5 1.0 ND1 A:HIS111 4.1 11.1 1.0 O A:HOH1076 4.1 18.3 0.5 CG A:HIS399 4.2 9.9 1.0 CG A:HIS111 4.2 10.7 1.0 CB A:PHE447 4.4 11.6 1.0 CG A:PRO79 4.5 11.3 1.0 CD2 A:HIS64 4.5 10.8 1.0 CU A:CU504 4.5 16.1 0.1 CG A:PHE447 4.6 11.6 1.0 NE2 A:HIS397 4.7 12.8 1.0 NE2 A:HIS64 4.7 11.8 1.0 CE2 A:PHE447 4.9 12.6 1.0 CG A:HIS397 4.9 12.3 1.0

Copper binding site 2 out of 4 in the T2-Depleted Laccase From Coriolopsis Caperata


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of T2-Depleted Laccase From Coriolopsis Caperata within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu502 b:9.6 occ:0.80 ND1 A:HIS66 2.0 10.6 1.0 NE2 A:HIS109 2.0 9.0 1.0 NE2 A:HIS451 2.1 11.5 1.0 O A:HOH612 2.8 9.1 0.5 CE1 A:HIS66 2.9 9.9 1.0 CE1 A:HIS451 3.0 11.8 1.0 CE1 A:HIS109 3.0 8.9 1.0 CD2 A:HIS109 3.0 9.9 1.0 CG A:HIS66 3.1 9.1 1.0 CD2 A:HIS451 3.2 11.3 1.0 CB A:HIS66 3.5 8.8 1.0 CZ2 A:TRP107 3.6 9.2 1.0 CD2 A:HIS64 3.6 10.8 1.0 CE2 A:TRP107 3.9 9.0 1.0 NE1 A:TRP107 4.0 9.6 1.0 CU A:CU504 4.0 16.1 0.1 NE2 A:HIS66 4.1 10.6 1.0 ND1 A:HIS109 4.1 9.0 1.0 CG A:HIS109 4.1 8.7 1.0 ND1 A:HIS451 4.1 11.4 1.0 CD2 A:HIS66 4.1 10.3 1.0 NE2 A:HIS64 4.2 11.8 1.0 CD2 A:HIS397 4.2 13.3 1.0 CB A:ALA240 4.2 9.5 1.0 CG A:HIS451 4.3 11.2 1.0 CH2 A:TRP107 4.4 10.1 1.0 NE2 A:HIS397 4.4 12.8 1.0 O A:HOH1076 4.6 18.3 0.5 CA A:HIS66 4.6 9.2 1.0 CG A:HIS64 4.8 10.4 1.0 CD2 A:TRP107 4.8 9.0 1.0 CG A:HIS397 5.0 12.3 1.0

Copper binding site 3 out of 4 in the T2-Depleted Laccase From Coriolopsis Caperata


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 3 of T2-Depleted Laccase From Coriolopsis Caperata within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu503 b:9.5 occ:0.80 ND1 A:HIS455 2.0 11.9 1.0 ND1 A:HIS394 2.0 11.9 1.0 SG A:CYS450 2.2 11.7 1.0 CE1 A:HIS394 2.9 12.3 1.0 CE1 A:HIS455 3.0 12.8 1.0 CG A:HIS455 3.0 11.7 1.0 CG A:HIS394 3.1 11.6 1.0 CB A:CYS450 3.3 10.7 1.0 CB A:HIS455 3.4 12.2 1.0 CB A:HIS394 3.5 11.2 1.0 CD1 A:ILE452 3.6 13.0 1.0 CB A:ILE452 3.8 12.6 1.0 CD2 A:PHE460 3.8 12.4 1.0 CA A:HIS394 4.0 10.6 1.0 CE2 A:PHE460 4.0 11.9 1.0 CG1 A:ILE452 4.1 12.7 1.0 NE2 A:HIS394 4.1 11.8 1.0 NE2 A:HIS455 4.1 13.6 1.0 CD2 A:HIS394 4.2 12.0 1.0 CD2 A:HIS455 4.2 12.8 1.0 CG2 A:ILE452 4.6 12.5 1.0 CD A:PRO395 4.7 10.2 1.0 CA A:CYS450 4.7 10.7 1.0 N A:ILE452 4.7 11.4 1.0 O A:GLY391 4.7 15.3 1.0 CA A:ILE452 4.8 12.1 1.0 CA A:HIS455 4.9 12.4 1.0 O A:ILE452 4.9 12.9 1.0 C A:HIS394 5.0 10.8 1.0 N A:HIS394 5.0 10.9 1.0

Copper binding site 4 out of 4 in the T2-Depleted Laccase From Coriolopsis Caperata


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 4 of T2-Depleted Laccase From Coriolopsis Caperata within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu504 b:16.1 occ:0.10 NE2 A:HIS397 1.5 12.8 1.0 NE2 A:HIS64 1.6 11.8 1.0 CD2 A:HIS397 2.5 13.3 1.0 CE1 A:HIS64 2.6 10.7 1.0 CE1 A:HIS397 2.6 12.0 1.0 CD2 A:HIS64 2.7 10.8 1.0 O A:HOH603 2.8 11.4 1.0 ND1 A:HIS66 3.4 10.6 1.0 NE2 A:HIS399 3.5 10.6 1.0 CE1 A:HIS399 3.5 10.7 1.0 CG A:HIS66 3.6 9.1 1.0 CG A:HIS397 3.6 12.3 1.0 ND1 A:HIS397 3.6 12.5 1.0 ND1 A:HIS64 3.7 10.6 1.0 O A:HOH612 3.7 9.1 0.5 ND1 A:HIS399 3.7 10.2 1.0 CE1 A:HIS66 3.8 9.9 1.0 CG A:HIS64 3.8 10.4 1.0 CD2 A:HIS399 3.8 9.7 1.0 CA A:HIS66 3.9 9.2 1.0 CG A:HIS399 3.9 9.9 1.0 CU A:CU502 4.0 9.6 0.8 CD2 A:HIS66 4.1 10.3 1.0 CB A:HIS66 4.1 8.8 1.0 NE2 A:HIS66 4.1 10.6 1.0 N A:GLY67 4.1 9.4 1.0 C A:HIS66 4.5 9.7 1.0 CU A:CU501 4.5 10.7 0.8 O A:HOH638 4.7 11.0 1.0 CA A:HIS399 4.7 10.3 1.0 CB A:HIS399 4.8 9.9 1.0 O A:HOH656 4.9 14.8 1.0 N A:HIS66 4.9 9.1 1.0

K.M.Polyakov, T.V.Fedorova, O.A.Glazunova, S.A.Kurzeev, L.G.Maloshenok, O.V.Koroleva. X-Ray Study of Copper Ions Incorporation in T2D Laccase From Coriolopsis Caperata To Be Published.