Copper in PDB 4hak: Multicopper Oxidase Cueo Mutant E506A

The structure of Multicopper Oxidase Cueo Mutant E506A, PDB code: 4hak was solved by H.Komori, K.Kataoka, T.Sakurai, Y.Higuchi, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.20 / 1.40
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	50.439, 90.867, 53.244, 90.00, 102.70, 90.00
R / Rfree (%)	15.9 / 18.4

The binding sites of Copper atom in the Multicopper Oxidase Cueo Mutant E506A (pdb code 4hak). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 4 binding sites of Copper where determined in the Multicopper Oxidase Cueo Mutant E506A, PDB code: 4hak:
Jump to Copper binding site number: 1; 2; 3; 4;

Copper binding site 1 out of 4 in the Multicopper Oxidase Cueo Mutant E506A


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of Multicopper Oxidase Cueo Mutant E506A within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu1001 b:10.4 occ:1.00 ND1 A:HIS505 2.0 9.8 1.0 ND1 A:HIS443 2.0 9.8 1.0 SG A:CYS500 2.2 10.3 1.0 CE1 A:HIS443 3.0 10.3 1.0 CE1 A:HIS505 3.0 9.4 1.0 CG A:HIS505 3.0 8.8 1.0 CG A:HIS443 3.1 9.7 1.0 CB A:CYS500 3.2 9.8 1.0 SD A:MET510 3.3 11.3 1.0 CB A:HIS505 3.4 9.4 1.0 CB A:HIS443 3.4 10.0 1.0 O A:LEU442 3.7 12.2 1.0 CA A:HIS443 3.7 9.8 1.0 CE A:MET510 4.0 11.9 1.0 CB A:LEU502 4.1 8.7 1.0 NE2 A:HIS443 4.1 10.6 1.0 NE2 A:HIS505 4.1 10.3 1.0 CD2 A:HIS505 4.1 9.5 1.0 CD2 A:HIS443 4.2 10.3 1.0 C A:LEU442 4.4 10.6 1.0 N A:HIS443 4.4 9.7 1.0 CA A:CYS500 4.6 9.7 1.0 CG A:MET510 4.8 12.3 1.0 CD1 A:LEU502 4.8 10.3 1.0 O A:LEU502 4.9 8.3 1.0 CA A:HIS505 4.9 8.8 1.0 C A:HIS443 4.9 9.7 1.0 CG A:LEU502 4.9 8.8 1.0 N A:LEU502 5.0 8.4 1.0

Copper binding site 2 out of 4 in the Multicopper Oxidase Cueo Mutant E506A


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of Multicopper Oxidase Cueo Mutant E506A within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu1002 b:16.3 occ:0.70 ND1 A:HIS103 2.0 12.7 1.0 NE2 A:HIS141 2.1 14.8 1.0 NE2 A:HIS501 2.1 13.4 1.0 O A:OH1005 2.5 22.8 0.7 CE1 A:HIS103 2.9 11.8 1.0 CE1 A:HIS141 3.0 14.8 1.0 CG A:HIS103 3.0 12.3 1.0 CE1 A:HIS501 3.1 13.9 1.0 CD2 A:HIS141 3.1 14.1 1.0 CD2 A:HIS501 3.1 13.8 1.0 CB A:HIS103 3.4 12.6 1.0 CZ2 A:TRP139 3.8 10.2 1.0 O A:HOH1101 4.0 23.0 0.5 CE2 A:TRP139 4.0 9.8 1.0 NE2 A:HIS103 4.1 11.9 1.0 CD2 A:HIS103 4.1 11.9 1.0 NE1 A:TRP139 4.1 10.9 1.0 ND1 A:HIS141 4.1 13.8 1.0 CU A:CU1004 4.2 19.9 0.5 ND1 A:HIS501 4.2 13.5 1.0 CG A:HIS141 4.2 10.7 1.0 CG A:HIS501 4.3 11.9 1.0 CD2 A:HIS101 4.3 17.8 1.0 CD2 A:HIS446 4.5 15.3 1.0 CU A:CU1003 4.5 17.4 0.7 CH2 A:TRP139 4.5 12.1 1.0 NE2 A:HIS446 4.5 15.7 1.0 CA A:HIS103 4.6 12.5 1.0 NE2 A:HIS101 4.7 18.8 1.0

Copper binding site 3 out of 4 in the Multicopper Oxidase Cueo Mutant E506A


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 3 of Multicopper Oxidase Cueo Mutant E506A within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu1003 b:17.4 occ:0.70 NE2 A:HIS448 2.0 17.7 1.0 NE2 A:HIS499 2.0 14.7 1.0 NE2 A:HIS143 2.0 19.4 1.0 O A:OH1005 2.1 22.8 0.7 CE1 A:HIS448 2.9 19.7 1.0 CD2 A:HIS499 2.9 14.4 1.0 CE1 A:HIS499 3.0 15.6 1.0 CD2 A:HIS143 3.0 18.6 1.0 CD2 A:HIS448 3.0 17.3 1.0 CE1 A:HIS143 3.0 19.1 1.0 O A:HOH1101 3.6 23.0 0.5 CD2 A:HIS446 3.6 15.3 1.0 CU A:CU1004 3.7 19.9 0.5 NE2 A:HIS101 3.9 18.8 1.0 CD2 A:HIS101 4.0 17.8 1.0 ND1 A:HIS448 4.0 18.0 1.0 ND1 A:HIS499 4.1 13.8 1.0 CG A:HIS499 4.1 13.1 1.0 CG A:HIS448 4.1 15.6 1.0 ND1 A:HIS143 4.1 18.5 1.0 NE2 A:HIS446 4.2 15.7 1.0 CG A:HIS143 4.2 16.4 1.0 CU A:CU1002 4.5 16.3 0.7 CE1 A:HIS101 4.7 18.6 1.0 CB A:MET497 4.7 15.6 1.0 CG A:HIS101 4.7 15.9 1.0 O A:HOH1102 4.9 26.9 0.5 CG A:HIS446 4.9 13.3 1.0 CD2 A:HIS501 4.9 13.8 1.0 CE1 A:HIS141 4.9 14.8 1.0 NE2 A:HIS501 5.0 13.4 1.0

Copper binding site 4 out of 4 in the Multicopper Oxidase Cueo Mutant E506A


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 4 of Multicopper Oxidase Cueo Mutant E506A within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu1004 b:19.9 occ:0.50 NE2 A:HIS446 1.9 15.7 1.0 NE2 A:HIS101 2.0 18.8 1.0 OXT A:ACT1006 2.2 31.4 0.5 O A:HOH1104 2.4 19.9 0.5 CD2 A:HIS446 2.9 15.3 1.0 CE1 A:HIS101 2.9 18.6 1.0 CE1 A:HIS446 3.0 15.6 1.0 CD2 A:HIS101 3.0 17.8 1.0 CD2 A:HIS448 3.2 17.3 1.0 NE2 A:HIS448 3.3 17.7 1.0 C A:ACT1006 3.4 31.2 0.5 CA A:HIS103 3.5 12.5 1.0 CG A:HIS103 3.5 12.3 1.0 O A:OH1005 3.6 22.8 0.7 CU A:CU1003 3.7 17.4 0.7 CB A:HIS103 3.7 12.6 1.0 ND1 A:HIS103 3.7 12.7 1.0 CG A:HIS448 3.8 15.6 1.0 CE1 A:HIS448 3.9 19.7 1.0 CG A:HIS446 4.0 13.3 1.0 ND1 A:HIS101 4.0 18.5 1.0 ND1 A:HIS446 4.0 15.2 1.0 O A:ACT1006 4.1 31.3 0.5 CD2 A:HIS103 4.1 11.9 1.0 CG A:HIS101 4.1 15.9 1.0 CU A:CU1002 4.2 16.3 0.7 ND1 A:HIS448 4.2 18.0 1.0 N A:GLY104 4.2 13.1 1.0 CE1 A:HIS103 4.3 11.8 1.0 N A:HIS103 4.3 12.4 1.0 C A:HIS103 4.4 12.4 1.0 NE2 A:HIS103 4.5 11.9 1.0 CH3 A:ACT1006 4.5 31.2 0.5 CA A:HIS448 4.5 13.9 1.0 CB A:HIS448 4.7 15.0 1.0 O A:TRP102 4.8 14.0 1.0 O A:HOH1106 4.8 9.9 0.5 O A:HOH1105 4.8 19.4 0.5 C A:TRP102 4.9 13.0 1.0 N A:HIS448 4.9 13.6 1.0

H.Komori, K.Kataoka, T.Sakurai, Y.Higuchi. Multicopper Oxidase Cueo Mutant E506A To Be Published.