Copper in PDB 4a2e: Crystal Structure of A Coriolopsis Gallica Laccase at 1.7 A Resolution pH 5.5

All present enzymatic activity of Crystal Structure of A Coriolopsis Gallica Laccase at 1.7 A Resolution pH 5.5:
1.10.3.2;

The structure of Crystal Structure of A Coriolopsis Gallica Laccase at 1.7 A Resolution pH 5.5, PDB code: 4a2e was solved by E.De La Mora, B.Valderrama, E.Horjales, E.Rudino-Pinera, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	25.63 / 1.80
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	56.385, 86.127, 152.637, 90.00, 90.00, 90.00
R / Rfree (%)	15.4 / 18.7

The binding sites of Copper atom in the Crystal Structure of A Coriolopsis Gallica Laccase at 1.7 A Resolution pH 5.5 (pdb code 4a2e). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 4 binding sites of Copper where determined in the Crystal Structure of A Coriolopsis Gallica Laccase at 1.7 A Resolution pH 5.5, PDB code: 4a2e:
Jump to Copper binding site number: 1; 2; 3; 4;

Copper binding site 1 out of 4 in the Crystal Structure of A Coriolopsis Gallica Laccase at 1.7 A Resolution pH 5.5


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of Crystal Structure of A Coriolopsis Gallica Laccase at 1.7 A Resolution pH 5.5 within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu1518 b:14.4 occ:0.80 ND1 A:HIS87 2.0 10.7 1.0 NE2 A:HIS472 2.1 15.2 1.0 NE2 A:HIS130 2.1 11.3 1.0 O A:HOH2200 2.7 24.7 1.0 CE1 A:HIS87 2.9 11.3 1.0 CG A:HIS87 3.1 9.6 1.0 CE1 A:HIS472 3.1 17.4 1.0 CE1 A:HIS130 3.1 11.3 1.0 CD2 A:HIS130 3.1 12.2 1.0 CD2 A:HIS472 3.2 14.7 1.0 CB A:HIS87 3.5 8.4 1.0 CZ2 A:TRP128 3.6 11.4 1.0 CD2 A:HIS85 3.6 10.2 1.0 CE2 A:TRP128 3.9 10.9 1.0 CU A:CU1520 4.0 17.3 0.5 NE2 A:HIS87 4.1 11.0 1.0 NE1 A:TRP128 4.1 11.1 1.0 CD2 A:HIS87 4.2 10.3 1.0 NE2 A:HIS85 4.2 12.1 1.0 ND1 A:HIS472 4.2 13.9 1.0 ND1 A:HIS130 4.2 10.8 1.0 CB A:ALA261 4.3 10.0 1.0 O A:HOH2205 4.3 32.1 1.0 CG A:HIS130 4.3 10.7 1.0 CG A:HIS472 4.3 17.6 1.0 CD2 A:HIS418 4.3 9.9 0.8 CH2 A:TRP128 4.3 10.7 1.0 NE2 A:HIS418 4.4 6.7 0.2 NE2 A:HIS418 4.5 12.1 0.8 CA A:HIS87 4.6 7.4 1.0 CE1 A:HIS418 4.7 7.4 0.2 CD2 A:HIS418 4.7 7.0 0.2 CG A:HIS85 4.8 11.6 1.0 CD2 A:TRP128 4.9 10.4 1.0

Copper binding site 2 out of 4 in the Crystal Structure of A Coriolopsis Gallica Laccase at 1.7 A Resolution pH 5.5


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of Crystal Structure of A Coriolopsis Gallica Laccase at 1.7 A Resolution pH 5.5 within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu1519 b:12.8 occ:0.81 NE2 A:HIS420 2.0 11.6 1.0 NE2 A:HIS470 2.0 18.8 1.0 NE2 A:HIS132 2.0 9.9 1.0 O A:HOH2200 2.3 24.7 1.0 NE2 A:HIS418 2.4 6.7 0.2 CE1 A:HIS420 2.8 10.2 1.0 CE1 A:HIS470 2.9 20.6 1.0 CD2 A:HIS132 3.0 9.3 1.0 CE1 A:HIS418 3.0 7.4 0.2 CE1 A:HIS132 3.0 13.2 1.0 CD2 A:HIS470 3.0 15.1 1.0 CD2 A:HIS420 3.2 11.3 1.0 CD2 A:HIS418 3.6 7.0 0.2 O A:HOH2205 3.7 32.1 1.0 CD2 A:HIS418 4.0 9.9 0.8 ND1 A:HIS420 4.0 9.2 1.0 ND1 A:HIS470 4.1 16.1 1.0 ND1 A:HIS132 4.1 9.5 1.0 CG A:HIS132 4.1 10.8 1.0 CG A:HIS470 4.1 14.7 1.0 CG A:HIS420 4.2 8.6 1.0 CD2 A:PHE468 4.2 11.0 1.0 ND1 A:HIS418 4.3 9.2 0.2 CB A:PHE468 4.4 8.5 1.0 CU A:CU1520 4.4 17.3 0.5 CD2 A:HIS85 4.5 10.2 1.0 CG A:HIS418 4.6 6.6 0.2 CG A:PRO100 4.6 11.4 1.0 NE2 A:HIS85 4.7 12.1 1.0 CG A:PHE468 4.7 8.7 1.0 NE2 A:HIS418 4.7 12.1 0.8 CD2 A:HIS472 5.0 14.7 1.0 NE2 A:HIS472 5.0 15.2 1.0

Copper binding site 3 out of 4 in the Crystal Structure of A Coriolopsis Gallica Laccase at 1.7 A Resolution pH 5.5


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 3 of Crystal Structure of A Coriolopsis Gallica Laccase at 1.7 A Resolution pH 5.5 within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu1520 b:17.3 occ:0.46 NE2 A:HIS85 1.7 12.1 1.0 NE2 A:HIS418 1.8 12.1 0.8 CD2 A:HIS418 2.7 9.9 0.8 CE1 A:HIS85 2.7 9.4 1.0 CD2 A:HIS85 2.8 10.2 1.0 O A:HOH2138 2.8 11.7 1.0 CE1 A:HIS418 2.9 7.7 0.8 ND1 A:HIS87 3.4 10.7 1.0 CE1 A:HIS420 3.4 10.2 1.0 CD2 A:HIS418 3.4 7.0 0.2 NE2 A:HIS420 3.4 11.6 1.0 O A:HOH2200 3.5 24.7 1.0 ND1 A:HIS420 3.6 9.2 1.0 CD2 A:HIS420 3.6 11.3 1.0 CG A:HIS87 3.7 9.6 1.0 CE1 A:HIS87 3.7 11.3 1.0 NE2 A:HIS418 3.7 6.7 0.2 CG A:HIS420 3.8 8.6 1.0 ND1 A:HIS85 3.8 10.4 1.0 CG A:HIS418 3.8 7.8 0.8 CG A:HIS85 3.9 11.6 1.0 ND1 A:HIS418 3.9 10.1 0.8 CU A:CU1518 4.0 14.4 0.8 CA A:HIS87 4.0 7.4 1.0 NE2 A:HIS87 4.1 11.0 1.0 CD2 A:HIS87 4.1 10.3 1.0 CB A:HIS87 4.2 8.4 1.0 N A:GLY88 4.3 8.1 1.0 CU A:CU1519 4.4 12.8 0.8 CA A:HIS420 4.6 8.7 1.0 CG A:HIS418 4.6 6.6 0.2 C A:HIS87 4.6 9.7 1.0 O A:HOH2141 4.7 12.6 1.0 CB A:HIS420 4.7 9.5 1.0 O A:HOH2516 4.9 14.2 1.0 CE1 A:HIS418 4.9 7.4 0.2

Copper binding site 4 out of 4 in the Crystal Structure of A Coriolopsis Gallica Laccase at 1.7 A Resolution pH 5.5


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 4 of Crystal Structure of A Coriolopsis Gallica Laccase at 1.7 A Resolution pH 5.5 within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu1521 b:12.7 occ:0.70 ND1 A:HIS415 2.0 14.3 1.0 ND1 A:HIS476 2.1 18.4 1.0 SG A:CYS471 2.2 14.2 1.0 CE1 A:HIS415 2.9 19.4 1.0 CG A:HIS415 3.0 14.4 1.0 CE1 A:HIS476 3.1 20.5 1.0 CG A:HIS476 3.1 19.2 1.0 CB A:CYS471 3.3 11.2 1.0 CD1 A:ILE473 3.4 15.4 1.0 CB A:HIS476 3.4 16.0 1.0 CB A:HIS415 3.4 12.5 1.0 CD2 A:PHE481 3.7 10.9 1.0 CB A:ILE473 3.8 15.8 1.0 CE2 A:PHE481 3.9 12.8 1.0 CA A:HIS415 4.0 12.0 1.0 CG1 A:ILE473 4.0 15.4 1.0 NE2 A:HIS415 4.1 14.4 1.0 CD2 A:HIS415 4.1 11.9 1.0 NE2 A:HIS476 4.2 18.9 1.0 CD2 A:HIS476 4.2 15.6 1.0 CG2 A:ILE473 4.6 16.6 1.0 CA A:CYS471 4.6 12.0 1.0 CD A:PRO416 4.7 9.7 1.0 O A:GLY412 4.8 16.4 1.0 N A:ILE473 4.8 17.3 1.0 CA A:ILE473 4.9 16.3 1.0 O A:ILE473 4.9 16.3 1.0 CA A:HIS476 4.9 16.1 1.0 C A:HIS415 4.9 10.5 1.0 CG A:PHE481 5.0 12.8 1.0

E.De La Mora, J.E.Lovett, C.F.Blanford, E.F.Garman, B.Valderrama, E.Rudino-Pinera. Structural Changes Caused By Radiation-Induced Reduction and Radiolysis: the Effect of X-Ray Absorbed Dose in A Fungal Multicopper Oxidase Acta Crystallogr.,Sect.D V. 68 564 2012.
ISSN: ISSN 0907-4449
PubMed: 22525754
DOI: 10.1107/S0907444912005343