The binding sites of Copper atom in the structure of Oxidized (CU2+) Peptidylglycine Alpha-Hydroxylating Monooxygenase (Phm) With Bound Azide Obtained By Co-Crystallization (pdb code 3mic). This binding sites where shown with 5.0 Angstroms radius around Copper atom. The 3mic structure was solved by E.E.CHUFAN, B.A.EIPPER, R.E.MAINS, L.M.AMZEL, with X-Ray Crystallography technique, brief refinement statistics is given in the table below:
Resolution (A) | 52.0-2.4 | Space group | P212121 | a (A) | 68.454 | b (A) | 68.579 | c (A) | 81.409 | alpha (°) | 90.00 | beta (°) | 90.00 | gamma (°) | 90.00 | Rfactor (%) | 19.6 | Rfree (%) | 26.4 |
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Copper binding site 1 out of 2 in 3mic
|  Click to enlarge |  Click to enlarge | Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 1 of Copper in the PDB 3mic. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Copper atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: A: Tyr79, A: His107, A: His108, A: His172, A: Hoh421, A: Hoh459, | conact list:
Atom | Atom | Distance (A) | Cu | OH A:Tyr79 | 4.82 | Cu | O A:His107 | 4.18 | Cu | NE2 A:His107 | 4.20 | Cu | CB A:His107 | 3.20 | Cu | ND1 A:His107 | 2.08 | Cu | CD2 A:His107 | 4.15 | Cu | C A:His107 | 3.63 | Cu | CE1 A:His107 | 3.13 | Cu | CG A:His107 | 2.97 | Cu | CA A:His107 | 4.04 | Cu | O A:His108 | 4.91 | Cu | NE2 A:His108 | 4.19 | Cu | N A:His108 | 3.35 | Cu | CB A:His108 | 3.34 | Cu | ND1 A:His108 | 2.11 | Cu | CD2 A:His108 | 4.19 | Cu | C A:His108 | 4.84 | Cu | CE1 A:His108 | 3.10 | Cu | CG A:His108 | 3.05 | Cu | CA A:His108 | 3.92 | Cu | O A:His172 | 4.73 | Cu | NE2 A:His172 | 4.08 | Cu | CB A:His172 | 3.62 | Cu | ND1 A:His172 | 2.07 | Cu | CD2 A:His172 | 4.23 | Cu | CE1 A:His172 | 2.91 | Cu | CG A:His172 | 3.18 | Cu | CA A:His172 | 4.93 | Cu | O A:Hoh421 | 4.21 | Cu | O A:Hoh459 | 3.82 |
| interactive model:
| Copper binding site 2 out of 2 in 3mic
|  Click to enlarge |  Click to enlarge | Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 2 of Copper in the PDB 3mic. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Copper atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: A: Glu128, A: His242, A: His244, A: Met314, A: Na360, A: Azi1, A: Hoh465, | conact list:
Atom | Atom | Distance (A) | Cu | OE2 A:Glu128 | 4.85 | Cu | NE2 A:His242 | 2.08 | Cu | ND1 A:His242 | 4.17 | Cu | CD2 A:His242 | 3.08 | Cu | CE1 A:His242 | 3.04 | Cu | CG A:His242 | 4.22 | Cu | NE2 A:His244 | 2.14 | Cu | ND1 A:His244 | 4.24 | Cu | CD2 A:His244 | 2.99 | Cu | CE1 A:His244 | 3.20 | Cu | CG A:His244 | 4.18 | Cu | CB A:Met314 | 3.81 | Cu | CE A:Met314 | 3.78 | Cu | CG A:Met314 | 3.57 | Cu | SD A:Met314 | 2.49 | Cu | NA A:Na360 | 3.84 | Cu | N1 A:Azi1 | 1.96 | Cu | N3 A:Azi1 | 3.65 | Cu | N2 A:Azi1 | 2.70 | Cu | O A:Hoh465 | 4.43 |
| interactive model:
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