Copper in the structure of Crystal Structure Analysis of the Copper-Reconstituted P19 Protein From Campylobacter Jejuni At 1.65 A At pH 10.0 (pdb 3lzo)

The binding sites of Copper atom in the structure of Crystal Structure Analysis of the Copper-Reconstituted P19 Protein From Campylobacter Jejuni At 1.65 A At pH 10.0 (pdb code 3lzo). This binding sites where shown with 5.0 Angstroms radius around Copper atom. The 3lzo structure was solved by T.I.DOUKOV, A.C.K.CHAN, M.SCOFIELD, A.B.RAMIN, S.A.L.TOM-YEW, M.E.P.MURPHY, with X-Ray Crystallography technique, brief refinement statistics is given in the table below: Resolution (A) 19.3-1.6 Space group P21212 a (A) 55.831 b (A) 72.581 c (A) 78.817 alpha (°) 90.00 beta (°) 90.00 gamma (°) 90.00 Rfactor (%) 13.9 Rfree (%) 18.7 Copper Binding Sites: Copper binding site 1 out of 2 in 3lzo Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 1 of Copper in the PDB 3lzo. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Copper atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: A: Met27, A: His42, A: Glu44, A: Met88, A: His95, A: Gly97, B: His132, conact list: Atom Atom Distance (A) Cu SD A:Met27 4.95 Cu NE2 A:His42 1.96 Cu ND1 A:His42 4.06 Cu CD2 A:His42 2.94 Cu CE1 A:His42 2.96 Cu CG A:His42 4.09 Cu OE1 A:Glu44 2.34 Cu OE1 A:Glu44 4.71 Cu CB A:Glu44 4.91 Cu OE2 A:Glu44 2.81 Cu OE2 A:Glu44 3.09 Cu CD A:Glu44 2.92 Cu CD A:Glu44 3.67 Cu CG A:Glu44 4.40 Cu CG A:Glu44 3.70 Cu CE A:Met88 4.15 Cu CG A:Met88 3.78 Cu SD A:Met88 2.74 Cu NE2 A:His95 2.00 Cu ND1 A:His95 4.11 Cu CD2 A:His95 2.97 Cu CE1 A:His95 3.01 Cu CG A:His95 4.12 Cu N A:Gly97 4.84 Cu CA A:Gly97 4.30 Cu NE2 B:His132 2.12 Cu ND1 B:His132 4.13 Cu CD2 B:His132 3.24 Cu CE1 B:His132 2.95 Cu CG B:His132 4.30 interactive model: Copper binding site 2 out of 2 in 3lzo Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 2 of Copper in the PDB 3lzo. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Copper atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: A: His132, B: His42, B: Glu44, B: Met88, B: His95, B: Gly97, B: Hoh1135, conact list: Atom Atom Distance (A) Cu NE2 A:His132 2.09 Cu ND1 A:His132 4.16 Cu CD2 A:His132 3.14 Cu CE1 A:His132 3.01 Cu CG A:His132 4.25 Cu NE2 B:His42 2.00 Cu ND1 B:His42 4.13 Cu CD2 B:His42 2.95 Cu CE1 B:His42 3.04 Cu CG B:His42 4.11 Cu OE1 B:Glu44 2.18 Cu OE2 B:Glu44 2.90 Cu CD B:Glu44 2.87 Cu CG B:Glu44 4.32 Cu CE B:Met88 4.13 Cu CG B:Met88 3.75 Cu SD B:Met88 2.77 Cu NE2 B:His95 2.03 Cu ND1 B:His95 4.12 Cu CD2 B:His95 3.00 Cu CE1 B:His95 3.02 Cu CG B:His95 4.14 Cu N B:Gly97 4.99 Cu CA B:Gly97 4.48 Cu O B:Hoh1135 4.87 interactive model: