Copper in PDB 3iud: CU2+-Bound Form of Pseudomonas Stutzeri L-Rhamnose Isomerase
Enzymatic activity of CU2+-Bound Form of Pseudomonas Stutzeri L-Rhamnose Isomerase
All present enzymatic activity of CU2+-Bound Form of Pseudomonas Stutzeri L-Rhamnose Isomerase:
5.3.1.14;
Protein crystallography data
The structure of CU2+-Bound Form of Pseudomonas Stutzeri L-Rhamnose Isomerase, PDB code: 3iud
was solved by
H.Yoshida,
M.Yamaji,
T.Ishii,
K.Izumori,
S.Kamitori,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
41.99 /
2.44
|
Space group
|
P 1 21 1
|
Cell size a, b, c (Å), α, β, γ (°)
|
74.687,
104.629,
114.979,
90.00,
108.18,
90.00
|
R / Rfree (%)
|
17.7 /
23.8
|
Copper Binding Sites:
The binding sites of Copper atom in the CU2+-Bound Form of Pseudomonas Stutzeri L-Rhamnose Isomerase
(pdb code 3iud). This binding sites where shown within
5.0 Angstroms radius around Copper atom.
In total 8 binding sites of Copper where determined in the
CU2+-Bound Form of Pseudomonas Stutzeri L-Rhamnose Isomerase, PDB code: 3iud:
Jump to Copper binding site number:
1;
2;
3;
4;
5;
6;
7;
8;
Copper binding site 1 out
of 8 in 3iud
Go back to
Copper Binding Sites List in 3iud
Copper binding site 1 out
of 8 in the CU2+-Bound Form of Pseudomonas Stutzeri L-Rhamnose Isomerase
Mono view
Stereo pair view
|
A full contact list of Copper with other atoms in the Cu binding
site number 1 of CU2+-Bound Form of Pseudomonas Stutzeri L-Rhamnose Isomerase within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Cu501
b:42.0
occ:0.50
|
ND1
|
A:HIS281
|
2.3
|
24.6
|
1.0
|
OE2
|
A:GLU219
|
2.4
|
24.5
|
1.0
|
OD2
|
A:ASP254
|
2.5
|
17.2
|
1.0
|
OD2
|
A:ASP327
|
2.7
|
19.9
|
1.0
|
CD
|
A:GLU219
|
3.2
|
22.8
|
1.0
|
CE1
|
A:HIS281
|
3.2
|
25.6
|
1.0
|
CG
|
A:ASP327
|
3.3
|
19.4
|
1.0
|
OE1
|
A:GLU219
|
3.3
|
25.9
|
1.0
|
CG
|
A:HIS281
|
3.3
|
24.6
|
1.0
|
O
|
A:HOH549
|
3.4
|
19.0
|
1.0
|
CG
|
A:ASP254
|
3.5
|
13.6
|
1.0
|
CB
|
A:ASP327
|
3.5
|
16.3
|
1.0
|
CB
|
A:HIS281
|
3.7
|
20.2
|
1.0
|
CB
|
A:ASP254
|
3.8
|
13.2
|
1.0
|
CE1
|
A:HIS257
|
4.0
|
10.4
|
1.0
|
CU
|
A:CU502
|
4.2
|
34.8
|
0.5
|
OD1
|
A:ASP327
|
4.3
|
22.6
|
1.0
|
NE2
|
A:HIS257
|
4.4
|
9.0
|
1.0
|
NE2
|
A:HIS281
|
4.4
|
26.3
|
1.0
|
CD2
|
A:HIS281
|
4.4
|
25.1
|
1.0
|
CG
|
A:GLU219
|
4.6
|
20.2
|
1.0
|
OD1
|
A:ASP254
|
4.6
|
15.6
|
1.0
|
ND1
|
A:HIS257
|
4.9
|
11.3
|
1.0
|
|
Copper binding site 2 out
of 8 in 3iud
Go back to
Copper Binding Sites List in 3iud
Copper binding site 2 out
of 8 in the CU2+-Bound Form of Pseudomonas Stutzeri L-Rhamnose Isomerase
Mono view
Stereo pair view
|
A full contact list of Copper with other atoms in the Cu binding
site number 2 of CU2+-Bound Form of Pseudomonas Stutzeri L-Rhamnose Isomerase within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Cu502
b:34.8
occ:0.50
|
NE2
|
A:HIS257
|
2.3
|
9.0
|
1.0
|
OD2
|
A:ASP289
|
2.4
|
22.3
|
1.0
|
O
|
A:HOH524
|
2.5
|
25.0
|
1.0
|
OD1
|
A:ASP289
|
2.8
|
23.7
|
1.0
|
O
|
A:HOH454
|
2.9
|
14.5
|
1.0
|
CG
|
A:ASP289
|
2.9
|
22.1
|
1.0
|
OD1
|
A:ASP291
|
3.0
|
19.2
|
1.0
|
CD2
|
A:HIS257
|
3.3
|
10.1
|
1.0
|
CE1
|
A:HIS257
|
3.3
|
10.4
|
1.0
|
OD2
|
A:ASP254
|
3.5
|
17.2
|
1.0
|
CG
|
A:ASP291
|
3.8
|
17.6
|
1.0
|
OD2
|
A:ASP291
|
3.9
|
19.4
|
1.0
|
CG
|
A:ASP254
|
4.0
|
13.6
|
1.0
|
OD1
|
A:ASP254
|
4.1
|
15.6
|
1.0
|
O
|
A:HOH549
|
4.1
|
19.0
|
1.0
|
CU
|
A:CU501
|
4.2
|
42.0
|
0.5
|
CB
|
A:ASP289
|
4.4
|
17.5
|
1.0
|
ND1
|
A:HIS257
|
4.4
|
11.3
|
1.0
|
CG
|
A:HIS257
|
4.4
|
13.2
|
1.0
|
OD1
|
A:ASP327
|
4.6
|
22.6
|
1.0
|
CE
|
A:LYS221
|
4.7
|
13.8
|
1.0
|
ND2
|
A:ASN283
|
4.8
|
13.9
|
1.0
|
CG
|
A:ASP327
|
4.8
|
19.4
|
1.0
|
OE2
|
A:GLU219
|
4.8
|
24.5
|
1.0
|
NZ
|
A:LYS221
|
4.8
|
15.2
|
1.0
|
NH2
|
B:ARG65
|
4.9
|
14.7
|
1.0
|
O
|
A:HOH572
|
4.9
|
37.8
|
1.0
|
OD2
|
A:ASP327
|
4.9
|
19.9
|
1.0
|
|
Copper binding site 3 out
of 8 in 3iud
Go back to
Copper Binding Sites List in 3iud
Copper binding site 3 out
of 8 in the CU2+-Bound Form of Pseudomonas Stutzeri L-Rhamnose Isomerase
Mono view
Stereo pair view
|
A full contact list of Copper with other atoms in the Cu binding
site number 3 of CU2+-Bound Form of Pseudomonas Stutzeri L-Rhamnose Isomerase within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Cu503
b:37.2
occ:0.50
|
OD2
|
B:ASP327
|
2.3
|
23.7
|
1.0
|
OD2
|
B:ASP254
|
2.4
|
11.0
|
1.0
|
ND1
|
B:HIS281
|
2.5
|
18.5
|
1.0
|
OE2
|
B:GLU219
|
2.7
|
20.3
|
1.0
|
OE1
|
B:GLU219
|
3.1
|
17.5
|
1.0
|
CD
|
B:GLU219
|
3.2
|
17.6
|
1.0
|
CG
|
B:ASP327
|
3.3
|
21.8
|
1.0
|
CG
|
B:HIS281
|
3.4
|
18.6
|
1.0
|
O
|
B:HOH539
|
3.4
|
31.0
|
1.0
|
CE1
|
B:HIS281
|
3.5
|
18.9
|
1.0
|
CG
|
B:ASP254
|
3.5
|
9.8
|
1.0
|
CB
|
B:HIS281
|
3.5
|
17.3
|
1.0
|
CB
|
B:ASP327
|
3.6
|
19.1
|
1.0
|
O
|
B:HOH675
|
3.9
|
25.0
|
1.0
|
CB
|
B:ASP254
|
3.9
|
9.9
|
1.0
|
CE1
|
B:HIS257
|
4.1
|
16.5
|
1.0
|
OD1
|
B:ASP327
|
4.4
|
22.7
|
1.0
|
CG
|
B:GLU219
|
4.5
|
17.9
|
1.0
|
CD2
|
B:HIS281
|
4.6
|
19.1
|
1.0
|
NE2
|
B:HIS281
|
4.6
|
18.2
|
1.0
|
OD1
|
B:ASP254
|
4.6
|
10.7
|
1.0
|
NE2
|
B:HIS257
|
4.6
|
16.9
|
1.0
|
CU
|
B:CU504
|
4.8
|
43.7
|
0.5
|
ND1
|
B:HIS257
|
4.9
|
16.1
|
1.0
|
CA
|
B:ASP254
|
5.0
|
10.7
|
1.0
|
|
Copper binding site 4 out
of 8 in 3iud
Go back to
Copper Binding Sites List in 3iud
Copper binding site 4 out
of 8 in the CU2+-Bound Form of Pseudomonas Stutzeri L-Rhamnose Isomerase
Mono view
Stereo pair view
|
A full contact list of Copper with other atoms in the Cu binding
site number 4 of CU2+-Bound Form of Pseudomonas Stutzeri L-Rhamnose Isomerase within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Cu504
b:43.7
occ:0.50
|
O
|
B:HOH675
|
2.1
|
25.0
|
1.0
|
NE2
|
B:HIS257
|
2.1
|
16.9
|
1.0
|
OD2
|
B:ASP289
|
2.3
|
28.1
|
1.0
|
OD1
|
B:ASP289
|
2.8
|
26.7
|
1.0
|
CG
|
B:ASP289
|
2.9
|
26.1
|
1.0
|
CE1
|
B:HIS257
|
3.1
|
16.5
|
1.0
|
OD1
|
B:ASP291
|
3.1
|
19.4
|
1.0
|
CD2
|
B:HIS257
|
3.2
|
15.2
|
1.0
|
O
|
B:HOH539
|
3.4
|
31.0
|
1.0
|
OD2
|
B:ASP291
|
3.8
|
22.6
|
1.0
|
OD2
|
B:ASP254
|
3.8
|
11.0
|
1.0
|
CG
|
B:ASP291
|
3.9
|
19.8
|
1.0
|
ND1
|
B:HIS257
|
4.2
|
16.1
|
1.0
|
CG
|
B:ASP254
|
4.2
|
9.8
|
1.0
|
CE
|
B:LYS221
|
4.3
|
12.1
|
1.0
|
CG
|
B:HIS257
|
4.3
|
15.4
|
1.0
|
OD1
|
B:ASP254
|
4.3
|
10.7
|
1.0
|
CB
|
B:ASP289
|
4.3
|
22.5
|
1.0
|
NZ
|
B:LYS221
|
4.3
|
15.3
|
1.0
|
CD
|
B:LYS221
|
4.8
|
10.2
|
1.0
|
NH2
|
A:ARG65
|
4.8
|
10.1
|
1.0
|
CU
|
B:CU503
|
4.8
|
37.2
|
0.5
|
OD2
|
B:ASP327
|
4.9
|
23.7
|
1.0
|
|
Copper binding site 5 out
of 8 in 3iud
Go back to
Copper Binding Sites List in 3iud
Copper binding site 5 out
of 8 in the CU2+-Bound Form of Pseudomonas Stutzeri L-Rhamnose Isomerase
Mono view
Stereo pair view
|
A full contact list of Copper with other atoms in the Cu binding
site number 5 of CU2+-Bound Form of Pseudomonas Stutzeri L-Rhamnose Isomerase within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
C:Cu505
b:28.9
occ:0.50
|
OD2
|
C:ASP254
|
2.5
|
9.7
|
1.0
|
ND1
|
C:HIS281
|
2.6
|
16.2
|
1.0
|
OD2
|
C:ASP327
|
2.6
|
17.4
|
1.0
|
OE2
|
C:GLU219
|
3.1
|
21.3
|
1.0
|
CG
|
C:ASP327
|
3.2
|
17.4
|
1.0
|
OE1
|
C:GLU219
|
3.2
|
22.3
|
1.0
|
CB
|
C:ASP327
|
3.4
|
14.9
|
1.0
|
CG
|
C:HIS281
|
3.4
|
18.2
|
1.0
|
CD
|
C:GLU219
|
3.5
|
21.9
|
1.0
|
CB
|
C:HIS281
|
3.5
|
18.1
|
1.0
|
CG
|
C:ASP254
|
3.6
|
13.2
|
1.0
|
CE1
|
C:HIS281
|
3.6
|
17.7
|
1.0
|
O
|
C:HOH472
|
3.9
|
16.5
|
1.0
|
CB
|
C:ASP254
|
3.9
|
11.6
|
1.0
|
CE1
|
C:HIS257
|
4.2
|
17.8
|
1.0
|
OD1
|
C:ASP327
|
4.3
|
19.6
|
1.0
|
O
|
C:HOH533
|
4.6
|
25.5
|
1.0
|
CD2
|
C:HIS281
|
4.6
|
16.9
|
1.0
|
OD1
|
C:ASP254
|
4.7
|
12.9
|
1.0
|
NE2
|
C:HIS281
|
4.7
|
15.0
|
1.0
|
CU
|
C:CU506
|
4.7
|
28.2
|
0.5
|
NE2
|
C:HIS257
|
4.7
|
19.6
|
1.0
|
CG
|
C:GLU219
|
4.9
|
19.4
|
1.0
|
CA
|
C:ASP327
|
4.9
|
14.9
|
1.0
|
|
Copper binding site 6 out
of 8 in 3iud
Go back to
Copper Binding Sites List in 3iud
Copper binding site 6 out
of 8 in the CU2+-Bound Form of Pseudomonas Stutzeri L-Rhamnose Isomerase
Mono view
Stereo pair view
|
A full contact list of Copper with other atoms in the Cu binding
site number 6 of CU2+-Bound Form of Pseudomonas Stutzeri L-Rhamnose Isomerase within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
C:Cu506
b:28.2
occ:0.50
|
O
|
C:HOH472
|
1.8
|
16.5
|
1.0
|
NE2
|
C:HIS257
|
2.1
|
19.6
|
1.0
|
OD1
|
C:ASP289
|
2.4
|
27.9
|
1.0
|
OD2
|
C:ASP289
|
2.5
|
23.5
|
1.0
|
CG
|
C:ASP289
|
2.8
|
26.0
|
1.0
|
OD1
|
C:ASP291
|
3.0
|
17.3
|
1.0
|
CE1
|
C:HIS257
|
3.0
|
17.8
|
1.0
|
CD2
|
C:HIS257
|
3.1
|
19.5
|
1.0
|
CG
|
C:ASP291
|
3.9
|
19.1
|
1.0
|
OD2
|
C:ASP254
|
3.9
|
9.7
|
1.0
|
OD2
|
C:ASP291
|
3.9
|
19.4
|
1.0
|
OD1
|
C:ASP254
|
4.1
|
12.9
|
1.0
|
ND1
|
C:HIS257
|
4.2
|
19.3
|
1.0
|
CG
|
C:ASP254
|
4.2
|
13.2
|
1.0
|
CB
|
C:ASP289
|
4.2
|
22.1
|
1.0
|
CG
|
C:HIS257
|
4.3
|
19.8
|
1.0
|
NZ
|
C:LYS221
|
4.4
|
14.1
|
1.0
|
CE
|
C:LYS221
|
4.4
|
13.7
|
1.0
|
NH1
|
D:ARG65
|
4.6
|
11.9
|
1.0
|
CU
|
C:CU505
|
4.7
|
28.9
|
0.5
|
CD
|
C:LYS221
|
4.8
|
13.2
|
1.0
|
|
Copper binding site 7 out
of 8 in 3iud
Go back to
Copper Binding Sites List in 3iud
Copper binding site 7 out
of 8 in the CU2+-Bound Form of Pseudomonas Stutzeri L-Rhamnose Isomerase
Mono view
Stereo pair view
|
A full contact list of Copper with other atoms in the Cu binding
site number 7 of CU2+-Bound Form of Pseudomonas Stutzeri L-Rhamnose Isomerase within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
D:Cu507
b:35.6
occ:0.50
|
ND1
|
D:HIS281
|
2.3
|
21.7
|
1.0
|
OD2
|
D:ASP254
|
2.5
|
23.7
|
1.0
|
OE2
|
D:GLU219
|
2.6
|
19.2
|
1.0
|
OD2
|
D:ASP327
|
2.6
|
21.8
|
1.0
|
CG
|
D:ASP327
|
3.2
|
18.0
|
1.0
|
CE1
|
D:HIS281
|
3.2
|
22.4
|
1.0
|
CG
|
D:HIS281
|
3.3
|
21.6
|
1.0
|
CD
|
D:GLU219
|
3.3
|
16.1
|
1.0
|
OE1
|
D:GLU219
|
3.4
|
18.7
|
1.0
|
CB
|
D:ASP327
|
3.5
|
16.3
|
1.0
|
CG
|
D:ASP254
|
3.5
|
20.3
|
1.0
|
CB
|
D:HIS281
|
3.6
|
18.6
|
1.0
|
CE1
|
D:HIS257
|
3.9
|
13.0
|
1.0
|
CB
|
D:ASP254
|
4.0
|
17.9
|
1.0
|
OD1
|
D:ASP327
|
4.1
|
20.0
|
1.0
|
CU
|
D:CU508
|
4.3
|
25.1
|
0.5
|
NE2
|
D:HIS281
|
4.3
|
23.6
|
1.0
|
CD2
|
D:HIS281
|
4.4
|
22.8
|
1.0
|
NE2
|
D:HIS257
|
4.5
|
14.1
|
1.0
|
OD1
|
D:ASP254
|
4.6
|
19.3
|
1.0
|
O
|
D:HOH577
|
4.7
|
19.9
|
1.0
|
ND1
|
D:HIS257
|
4.8
|
13.3
|
1.0
|
CG
|
D:GLU219
|
4.8
|
15.1
|
1.0
|
|
Copper binding site 8 out
of 8 in 3iud
Go back to
Copper Binding Sites List in 3iud
Copper binding site 8 out
of 8 in the CU2+-Bound Form of Pseudomonas Stutzeri L-Rhamnose Isomerase
Mono view
Stereo pair view
|
A full contact list of Copper with other atoms in the Cu binding
site number 8 of CU2+-Bound Form of Pseudomonas Stutzeri L-Rhamnose Isomerase within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
D:Cu508
b:25.1
occ:0.50
|
NE2
|
D:HIS257
|
2.1
|
14.1
|
1.0
|
OD2
|
D:ASP289
|
2.3
|
18.4
|
1.0
|
OD1
|
D:ASP289
|
2.5
|
17.4
|
1.0
|
CG
|
D:ASP289
|
2.7
|
17.1
|
1.0
|
OD1
|
D:ASP291
|
2.7
|
20.5
|
1.0
|
CE1
|
D:HIS257
|
3.1
|
13.0
|
1.0
|
CD2
|
D:HIS257
|
3.1
|
12.7
|
1.0
|
O
|
D:HOH487
|
3.2
|
23.1
|
1.0
|
OD2
|
D:ASP254
|
3.4
|
23.7
|
1.0
|
CG
|
D:ASP291
|
3.6
|
16.6
|
1.0
|
OD2
|
D:ASP291
|
3.8
|
19.1
|
1.0
|
OD1
|
D:ASP254
|
3.8
|
19.3
|
1.0
|
CG
|
D:ASP254
|
3.8
|
20.3
|
1.0
|
ND1
|
D:HIS257
|
4.2
|
13.3
|
1.0
|
CG
|
D:HIS257
|
4.2
|
15.7
|
1.0
|
CB
|
D:ASP289
|
4.2
|
15.5
|
1.0
|
CU
|
D:CU507
|
4.3
|
35.6
|
0.5
|
ND2
|
D:ASN283
|
4.5
|
18.7
|
1.0
|
NZ
|
D:LYS221
|
4.6
|
21.9
|
1.0
|
OD1
|
D:ASP327
|
4.7
|
20.0
|
1.0
|
O
|
D:HOH500
|
4.8
|
15.7
|
1.0
|
CE
|
D:LYS221
|
4.9
|
18.1
|
1.0
|
CG
|
D:ASP327
|
4.9
|
18.0
|
1.0
|
NH2
|
C:ARG65
|
4.9
|
6.7
|
1.0
|
CB
|
D:ASP254
|
5.0
|
17.9
|
1.0
|
|
Reference:
H.Yoshida,
M.Yamaji,
T.Ishii,
K.Izumori,
S.Kamitori.
Catalytic Reaction Mechanism of Pseudomonas Stutzeri L-Rhamnose Isomerase Deduced From X-Ray Structures Febs J. V. 277 1045 2010.
ISSN: ISSN 1742-464X
PubMed: 20088877
DOI: 10.1111/J.1742-4658.2009.07548.X
Page generated: Wed Jul 31 01:12:59 2024
|