Copper in PDB 3ft0: Pseudomonas Aeruginosa Azurin with Mutated Metal-Binding Loop Sequence (Caaaahaaaam), Chemically Reduced

The structure of Pseudomonas Aeruginosa Azurin with Mutated Metal-Binding Loop Sequence (Caaaahaaaam), Chemically Reduced, PDB code: 3ft0 was solved by M.J.Banfield, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	21.75 / 1.80
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	34.664, 86.989, 42.156, 90.00, 114.67, 90.00
R / Rfree (%)	18.9 / 25

The binding sites of Copper atom in the Pseudomonas Aeruginosa Azurin with Mutated Metal-Binding Loop Sequence (Caaaahaaaam), Chemically Reduced (pdb code 3ft0). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 2 binding sites of Copper where determined in the Pseudomonas Aeruginosa Azurin with Mutated Metal-Binding Loop Sequence (Caaaahaaaam), Chemically Reduced, PDB code: 3ft0:
Jump to Copper binding site number: 1; 2;

Copper binding site 1 out of 2 in the Pseudomonas Aeruginosa Azurin with Mutated Metal-Binding Loop Sequence (Caaaahaaaam), Chemically Reduced


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of Pseudomonas Aeruginosa Azurin with Mutated Metal-Binding Loop Sequence (Caaaahaaaam), Chemically Reduced within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu501 b:17.0 occ:1.00 ND1 A:HIS117 2.1 15.1 1.0 ND1 A:HIS46 2.1 11.6 1.0 SG A:CYS112 2.2 13.8 1.0 CE1 A:HIS117 3.1 12.2 1.0 CG A:HIS117 3.1 14.6 1.0 CE1 A:HIS46 3.1 8.7 1.0 CG A:HIS46 3.1 11.2 1.0 CB A:CYS112 3.2 12.5 1.0 SD A:MET122 3.3 13.5 1.0 O A:GLY45 3.3 13.3 1.0 CA A:HIS46 3.4 12.8 1.0 CB A:HIS117 3.4 16.9 1.0 CB A:HIS46 3.4 12.8 1.0 CE A:MET122 3.8 13.8 1.0 CB A:ALA114 3.9 15.3 1.0 C A:GLY45 4.1 13.3 1.0 NE2 A:HIS117 4.2 12.6 1.0 N A:HIS46 4.2 13.0 1.0 CD2 A:HIS117 4.2 15.3 1.0 NE2 A:HIS46 4.2 9.6 1.0 CD2 A:HIS46 4.2 10.7 1.0 C A:HIS46 4.5 12.8 1.0 N A:ASN47 4.6 12.5 1.0 CA A:CYS112 4.6 12.7 1.0 N A:ALA114 4.8 14.6 1.0 CG A:MET122 4.8 13.8 1.0 CA A:HIS117 4.9 16.3 1.0 CA A:ALA114 4.9 15.1 1.0 CB A:MET122 4.9 13.1 1.0

Copper binding site 2 out of 2 in the Pseudomonas Aeruginosa Azurin with Mutated Metal-Binding Loop Sequence (Caaaahaaaam), Chemically Reduced


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of Pseudomonas Aeruginosa Azurin with Mutated Metal-Binding Loop Sequence (Caaaahaaaam), Chemically Reduced within 5.0Å range: probe atom residue distance (Å) B Occ B:Cu501 b:18.8 occ:1.00 ND1 B:HIS117 2.1 11.8 1.0 ND1 B:HIS46 2.1 11.6 1.0 SG B:CYS112 2.1 12.6 1.0 CE1 B:HIS117 3.1 13.1 1.0 CG B:HIS117 3.1 14.1 1.0 CE1 B:HIS46 3.1 12.4 1.0 CG B:HIS46 3.1 13.6 1.0 CB B:CYS112 3.2 11.9 1.0 SD B:MET122 3.2 14.4 1.0 O B:GLY45 3.4 15.9 1.0 CA B:HIS46 3.4 14.4 1.0 CB B:HIS117 3.4 14.9 1.0 CB B:HIS46 3.4 14.2 1.0 CE B:MET122 3.7 10.9 1.0 CB B:ALA114 3.8 13.2 1.0 NE2 B:HIS117 4.1 14.7 1.0 C B:GLY45 4.2 16.5 1.0 CD2 B:HIS117 4.2 14.8 1.0 N B:HIS46 4.2 15.3 1.0 NE2 B:HIS46 4.2 12.4 1.0 CD2 B:HIS46 4.2 13.7 1.0 N B:ASN47 4.5 13.3 1.0 C B:HIS46 4.5 13.9 1.0 CA B:CYS112 4.6 12.1 1.0 CG B:MET122 4.7 14.2 1.0 N B:ALA114 4.8 12.6 1.0 CA B:ALA114 4.8 13.9 1.0 CB B:MET122 4.9 14.8 1.0 CA B:HIS117 4.9 14.1 1.0 C B:CYS112 5.0 11.8 1.0

K.Sato, C.Li, I.Salard, A.J.Thompson, M.J.Banfield, C.Dennison. Metal-Binding Loop Length and Not Sequence Dictates Structure. Proc.Natl.Acad.Sci.Usa V. 106 5616 2009.
ISSN: ISSN 0027-8424
PubMed: 19299503
DOI: 10.1073/PNAS.0811324106