Copper in PDB 3fsz: Pseudomonas Aeruginosa Azurin with Mutated Metal-Binding Loop Sequence (Caaaahaaaam)

The structure of Pseudomonas Aeruginosa Azurin with Mutated Metal-Binding Loop Sequence (Caaaahaaaam), PDB code: 3fsz was solved by M.J.Banfield, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	21.44 / 2.00
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	34.824, 86.959, 41.848, 90.00, 113.82, 90.00
R / Rfree (%)	18.4 / 25.5

The binding sites of Copper atom in the Pseudomonas Aeruginosa Azurin with Mutated Metal-Binding Loop Sequence (Caaaahaaaam) (pdb code 3fsz). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 2 binding sites of Copper where determined in the Pseudomonas Aeruginosa Azurin with Mutated Metal-Binding Loop Sequence (Caaaahaaaam), PDB code: 3fsz:
Jump to Copper binding site number: 1; 2;

Copper binding site 1 out of 2 in the Pseudomonas Aeruginosa Azurin with Mutated Metal-Binding Loop Sequence (Caaaahaaaam)


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of Pseudomonas Aeruginosa Azurin with Mutated Metal-Binding Loop Sequence (Caaaahaaaam) within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu501 b:14.2 occ:1.00 SG A:CYS112 2.1 10.8 1.0 ND1 A:HIS117 2.1 9.4 1.0 ND1 A:HIS46 2.2 8.4 1.0 CG A:HIS117 3.1 9.9 1.0 CE1 A:HIS117 3.1 9.8 1.0 CE1 A:HIS46 3.1 8.2 1.0 CB A:CYS112 3.2 10.5 1.0 CG A:HIS46 3.2 9.5 1.0 SD A:MET122 3.3 13.5 1.0 O A:GLY45 3.3 10.8 1.0 CB A:HIS117 3.4 11.6 1.0 CA A:HIS46 3.4 10.5 1.0 CB A:HIS46 3.6 10.2 1.0 CE A:MET122 3.8 10.4 1.0 CB A:ALA114 3.9 12.2 1.0 C A:GLY45 4.1 11.7 1.0 N A:HIS46 4.2 10.7 1.0 NE2 A:HIS117 4.2 8.2 1.0 CD2 A:HIS117 4.2 10.4 1.0 NE2 A:HIS46 4.2 7.8 1.0 CD2 A:HIS46 4.3 8.5 1.0 N A:ASN47 4.5 10.2 1.0 C A:HIS46 4.5 10.1 1.0 CA A:CYS112 4.6 10.6 1.0 CG A:MET122 4.7 11.8 1.0 N A:ALA114 4.9 11.8 1.0 CB A:MET122 4.9 12.2 1.0 CA A:HIS117 4.9 11.7 1.0 CA A:ALA114 4.9 12.8 1.0

Copper binding site 2 out of 2 in the Pseudomonas Aeruginosa Azurin with Mutated Metal-Binding Loop Sequence (Caaaahaaaam)


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of Pseudomonas Aeruginosa Azurin with Mutated Metal-Binding Loop Sequence (Caaaahaaaam) within 5.0Å range: probe atom residue distance (Å) B Occ B:Cu501 b:14.2 occ:1.00 ND1 B:HIS46 2.0 8.2 1.0 ND1 B:HIS117 2.1 10.7 1.0 SG B:CYS112 2.2 9.5 1.0 CE1 B:HIS46 3.0 5.6 1.0 CG B:HIS46 3.0 9.4 1.0 CG B:HIS117 3.1 13.4 1.0 CE1 B:HIS117 3.1 12.6 1.0 SD B:MET122 3.2 12.2 1.0 O B:GLY45 3.2 10.6 1.0 CB B:CYS112 3.2 10.3 1.0 CA B:HIS46 3.4 10.5 1.0 CB B:HIS46 3.4 10.2 1.0 CB B:HIS117 3.4 13.9 1.0 CE B:MET122 3.7 9.6 1.0 CB B:ALA114 3.9 12.3 1.0 C B:GLY45 4.0 11.5 1.0 NE2 B:HIS46 4.1 5.2 1.0 N B:HIS46 4.1 10.4 1.0 CD2 B:HIS46 4.1 7.3 1.0 NE2 B:HIS117 4.2 11.0 1.0 CD2 B:HIS117 4.2 12.6 1.0 C B:HIS46 4.6 9.8 1.0 CA B:CYS112 4.6 10.0 1.0 CG B:MET122 4.6 11.3 1.0 N B:ASN47 4.6 9.6 1.0 CB B:MET122 4.9 11.1 1.0 CA B:HIS117 4.9 14.4 1.0 N B:ALA114 4.9 12.2 1.0 CA B:ALA114 5.0 13.2 1.0

K.Sato, C.Li, I.Salard, A.J.Thompson, M.J.Banfield, C.Dennison. Metal-Binding Loop Length and Not Sequence Dictates Structure. Proc.Natl.Acad.Sci.Usa V. 106 5616 2009.
ISSN: ISSN 0027-8424
PubMed: 19299503
DOI: 10.1073/PNAS.0811324106