Atomistry » Copper » PDB 3awt-3erx » 3azu
Atomistry »
  Copper »
    PDB 3awt-3erx »
      3azu »

Copper in PDB 3azu: X-Ray Crystal Structure of the Two Site-Specific Mutants HIS35GLN and HIS35LEU of Azurin From Pseudomonas Aeruginosa

Protein crystallography data

The structure of X-Ray Crystal Structure of the Two Site-Specific Mutants HIS35GLN and HIS35LEU of Azurin From Pseudomonas Aeruginosa, PDB code: 3azu was solved by A.Messerschmidt, H.Nar, R.Huber, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) N/A / 2.10
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 109.740, 99.150, 47.820, 90.00, 90.00, 90.00
R / Rfree (%) 16.3 / n/a

Copper Binding Sites:

The binding sites of Copper atom in the X-Ray Crystal Structure of the Two Site-Specific Mutants HIS35GLN and HIS35LEU of Azurin From Pseudomonas Aeruginosa (pdb code 3azu). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 4 binding sites of Copper where determined in the X-Ray Crystal Structure of the Two Site-Specific Mutants HIS35GLN and HIS35LEU of Azurin From Pseudomonas Aeruginosa, PDB code: 3azu:
Jump to Copper binding site number: 1; 2; 3; 4;

Copper binding site 1 out of 4 in 3azu

Go back to Copper Binding Sites List in 3azu
Copper binding site 1 out of 4 in the X-Ray Crystal Structure of the Two Site-Specific Mutants HIS35GLN and HIS35LEU of Azurin From Pseudomonas Aeruginosa


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of X-Ray Crystal Structure of the Two Site-Specific Mutants HIS35GLN and HIS35LEU of Azurin From Pseudomonas Aeruginosa within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cu129

b:21.9
occ:1.00
ND1 A:HIS46 2.0 29.8 1.0
SG A:CYS112 2.1 8.9 1.0
ND1 A:HIS117 2.1 17.5 1.0
CE1 A:HIS46 2.9 16.8 1.0
SD A:MET121 3.0 18.0 1.0
CG A:HIS46 3.0 27.7 1.0
CG A:HIS117 3.0 35.6 1.0
O A:GLY45 3.0 7.3 1.0
CE1 A:HIS117 3.1 9.9 1.0
CB A:CYS112 3.3 2.0 1.0
CB A:HIS117 3.3 5.5 1.0
CB A:HIS46 3.4 11.4 1.0
CA A:HIS46 3.4 10.6 1.0
C A:GLY45 3.9 4.1 1.0
CE A:MET121 4.0 2.0 1.0
NE2 A:HIS46 4.0 15.6 1.0
CB A:PHE114 4.1 2.0 1.0
CD2 A:HIS46 4.1 10.2 1.0
N A:HIS46 4.1 8.8 1.0
NE2 A:HIS117 4.2 30.4 1.0
CD2 A:HIS117 4.2 30.0 1.0
CG A:MET121 4.5 5.5 1.0
CA A:CYS112 4.6 33.8 1.0
C A:HIS46 4.7 5.5 1.0
N A:ASN47 4.8 16.3 1.0
CA A:HIS117 4.8 2.0 1.0
O A:MET44 4.9 9.6 1.0
CG A:PHE114 4.9 18.8 1.0
CB A:MET121 5.0 11.6 1.0
N A:PHE114 5.0 12.7 1.0

Copper binding site 2 out of 4 in 3azu

Go back to Copper Binding Sites List in 3azu
Copper binding site 2 out of 4 in the X-Ray Crystal Structure of the Two Site-Specific Mutants HIS35GLN and HIS35LEU of Azurin From Pseudomonas Aeruginosa


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of X-Ray Crystal Structure of the Two Site-Specific Mutants HIS35GLN and HIS35LEU of Azurin From Pseudomonas Aeruginosa within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Cu129

b:11.3
occ:1.00
SG B:CYS112 2.0 4.7 1.0
ND1 B:HIS46 2.0 2.0 1.0
ND1 B:HIS117 2.0 21.0 1.0
CE1 B:HIS117 3.0 8.6 1.0
CE1 B:HIS46 3.0 34.4 1.0
CG B:HIS117 3.0 2.0 1.0
CG B:HIS46 3.0 2.0 1.0
O B:GLY45 3.1 19.4 1.0
SD B:MET121 3.2 14.8 1.0
CB B:CYS112 3.2 2.0 1.0
CA B:HIS46 3.2 34.5 1.0
CB B:HIS117 3.4 12.8 1.0
CB B:HIS46 3.4 3.5 1.0
C B:GLY45 3.9 29.6 1.0
N B:HIS46 4.0 8.0 1.0
CB B:PHE114 4.0 2.0 1.0
CE B:MET121 4.1 2.0 1.0
NE2 B:HIS117 4.1 28.1 1.0
NE2 B:HIS46 4.2 17.9 1.0
CD2 B:HIS117 4.2 16.1 1.0
CD2 B:HIS46 4.2 22.0 1.0
C B:HIS46 4.4 2.0 1.0
N B:ASN47 4.5 23.2 1.0
CA B:CYS112 4.6 18.0 1.0
CG B:MET121 4.7 2.0 1.0
CG B:PHE114 4.7 11.3 1.0
O B:MET44 4.9 15.7 1.0
CA B:HIS117 4.9 7.6 1.0
C B:CYS112 5.0 5.5 1.0
N B:PHE114 5.0 14.0 1.0

Copper binding site 3 out of 4 in 3azu

Go back to Copper Binding Sites List in 3azu
Copper binding site 3 out of 4 in the X-Ray Crystal Structure of the Two Site-Specific Mutants HIS35GLN and HIS35LEU of Azurin From Pseudomonas Aeruginosa


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 3 of X-Ray Crystal Structure of the Two Site-Specific Mutants HIS35GLN and HIS35LEU of Azurin From Pseudomonas Aeruginosa within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Cu129

b:17.2
occ:1.00
ND1 C:HIS117 2.0 22.4 1.0
ND1 C:HIS46 2.0 6.5 1.0
SG C:CYS112 2.1 7.2 1.0
CG C:HIS117 3.0 2.9 1.0
CE1 C:HIS117 3.0 2.0 1.0
CE1 C:HIS46 3.0 5.8 1.0
CG C:HIS46 3.0 7.1 1.0
O C:GLY45 3.0 7.7 1.0
SD C:MET121 3.1 12.5 1.0
CB C:CYS112 3.2 3.4 1.0
CA C:HIS46 3.4 2.0 1.0
CB C:HIS117 3.4 13.6 1.0
CB C:HIS46 3.4 2.1 1.0
CE C:MET121 3.8 2.0 1.0
C C:GLY45 3.9 6.9 1.0
CB C:PHE114 4.0 12.5 1.0
N C:HIS46 4.0 5.2 1.0
NE2 C:HIS117 4.1 9.0 1.0
NE2 C:HIS46 4.1 2.1 1.0
CD2 C:HIS117 4.2 12.5 1.0
CD2 C:HIS46 4.2 7.3 1.0
C C:HIS46 4.5 23.1 1.0
CG C:MET121 4.5 12.0 1.0
CA C:CYS112 4.5 2.0 1.0
N C:ASN47 4.6 14.2 1.0
CG C:PHE114 4.7 8.6 1.0
CB C:MET121 4.9 11.8 1.0
CA C:HIS117 4.9 2.0 1.0
C C:CYS112 4.9 10.1 1.0
N C:PHE114 4.9 5.1 1.0

Copper binding site 4 out of 4 in 3azu

Go back to Copper Binding Sites List in 3azu
Copper binding site 4 out of 4 in the X-Ray Crystal Structure of the Two Site-Specific Mutants HIS35GLN and HIS35LEU of Azurin From Pseudomonas Aeruginosa


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 4 of X-Ray Crystal Structure of the Two Site-Specific Mutants HIS35GLN and HIS35LEU of Azurin From Pseudomonas Aeruginosa within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Cu129

b:17.6
occ:1.00
ND1 D:HIS46 2.0 5.0 1.0
SG D:CYS112 2.0 9.3 1.0
ND1 D:HIS117 2.0 25.3 1.0
SD D:MET121 2.9 16.8 1.0
CE1 D:HIS117 3.0 10.5 1.0
CE1 D:HIS46 3.0 69.5 1.0
CG D:HIS46 3.0 41.2 1.0
CG D:HIS117 3.0 10.5 1.0
O D:GLY45 3.2 20.3 1.0
CB D:CYS112 3.3 12.1 1.0
CA D:HIS46 3.4 10.5 1.0
CB D:HIS117 3.4 4.7 1.0
CB D:HIS46 3.4 2.0 1.0
CE D:MET121 3.9 2.4 1.0
CB D:PHE114 3.9 2.7 1.0
C D:GLY45 4.0 13.8 1.0
NE2 D:HIS117 4.1 14.7 1.0
NE2 D:HIS46 4.1 30.8 1.0
N D:HIS46 4.1 5.1 1.0
CD2 D:HIS46 4.2 27.1 1.0
CD2 D:HIS117 4.2 18.2 1.0
CG D:MET121 4.5 6.5 1.0
C D:HIS46 4.5 6.8 1.0
N D:ASN47 4.6 9.4 1.0
CA D:CYS112 4.6 20.1 1.0
CG D:PHE114 4.7 14.3 1.0
N D:PHE114 4.8 13.6 1.0
O D:MET44 4.9 20.2 1.0
CA D:HIS117 4.9 29.0 1.0
CB D:MET121 4.9 22.8 1.0
CA D:PHE114 5.0 14.2 1.0

Reference:

H.Nar, A.Messerschmidt, R.Huber, M.Van De Kamp, G.W.Canters. X-Ray Crystal Structure of the Two Site-Specific Mutants HIS35GLN and HIS35LEU of Azurin From Pseudomonas Aeruginosa. J.Mol.Biol. V. 218 427 1991.
ISSN: ISSN 0022-2836
PubMed: 1901363
DOI: 10.1016/0022-2836(91)90723-J
Page generated: Sun Dec 13 11:09:11 2020

Last articles

Zn in 7O75
Zn in 7O73
Zn in 7O4I
Zn in 7O72
Zn in 7O4J
Zn in 7NVR
Zn in 7NVY
Zn in 7NVZ
Zn in 7NW0
Zn in 7O4K
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy