Copper in PDB 3azu: X-Ray Crystal Structure of the Two Site-Specific Mutants HIS35GLN and HIS35LEU of Azurin From Pseudomonas Aeruginosa

The structure of X-Ray Crystal Structure of the Two Site-Specific Mutants HIS35GLN and HIS35LEU of Azurin From Pseudomonas Aeruginosa, PDB code: 3azu was solved by A.Messerschmidt, H.Nar, R.Huber, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	N/A / 2.10
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	109.740, 99.150, 47.820, 90.00, 90.00, 90.00
R / Rfree (%)	16.3 / n/a

The binding sites of Copper atom in the X-Ray Crystal Structure of the Two Site-Specific Mutants HIS35GLN and HIS35LEU of Azurin From Pseudomonas Aeruginosa (pdb code 3azu). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 4 binding sites of Copper where determined in the X-Ray Crystal Structure of the Two Site-Specific Mutants HIS35GLN and HIS35LEU of Azurin From Pseudomonas Aeruginosa, PDB code: 3azu:
Jump to Copper binding site number: 1; 2; 3; 4;

Copper binding site 1 out of 4 in the X-Ray Crystal Structure of the Two Site-Specific Mutants HIS35GLN and HIS35LEU of Azurin From Pseudomonas Aeruginosa


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of X-Ray Crystal Structure of the Two Site-Specific Mutants HIS35GLN and HIS35LEU of Azurin From Pseudomonas Aeruginosa within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu129 b:21.9 occ:1.00 ND1 A:HIS46 2.0 29.8 1.0 SG A:CYS112 2.1 8.9 1.0 ND1 A:HIS117 2.1 17.5 1.0 CE1 A:HIS46 2.9 16.8 1.0 SD A:MET121 3.0 18.0 1.0 CG A:HIS46 3.0 27.7 1.0 CG A:HIS117 3.0 35.6 1.0 O A:GLY45 3.0 7.3 1.0 CE1 A:HIS117 3.1 9.9 1.0 CB A:CYS112 3.3 2.0 1.0 CB A:HIS117 3.3 5.5 1.0 CB A:HIS46 3.4 11.4 1.0 CA A:HIS46 3.4 10.6 1.0 C A:GLY45 3.9 4.1 1.0 CE A:MET121 4.0 2.0 1.0 NE2 A:HIS46 4.0 15.6 1.0 CB A:PHE114 4.1 2.0 1.0 CD2 A:HIS46 4.1 10.2 1.0 N A:HIS46 4.1 8.8 1.0 NE2 A:HIS117 4.2 30.4 1.0 CD2 A:HIS117 4.2 30.0 1.0 CG A:MET121 4.5 5.5 1.0 CA A:CYS112 4.6 33.8 1.0 C A:HIS46 4.7 5.5 1.0 N A:ASN47 4.8 16.3 1.0 CA A:HIS117 4.8 2.0 1.0 O A:MET44 4.9 9.6 1.0 CG A:PHE114 4.9 18.8 1.0 CB A:MET121 5.0 11.6 1.0 N A:PHE114 5.0 12.7 1.0

Copper binding site 2 out of 4 in the X-Ray Crystal Structure of the Two Site-Specific Mutants HIS35GLN and HIS35LEU of Azurin From Pseudomonas Aeruginosa


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of X-Ray Crystal Structure of the Two Site-Specific Mutants HIS35GLN and HIS35LEU of Azurin From Pseudomonas Aeruginosa within 5.0Å range: probe atom residue distance (Å) B Occ B:Cu129 b:11.3 occ:1.00 SG B:CYS112 2.0 4.7 1.0 ND1 B:HIS46 2.0 2.0 1.0 ND1 B:HIS117 2.0 21.0 1.0 CE1 B:HIS117 3.0 8.6 1.0 CE1 B:HIS46 3.0 34.4 1.0 CG B:HIS117 3.0 2.0 1.0 CG B:HIS46 3.0 2.0 1.0 O B:GLY45 3.1 19.4 1.0 SD B:MET121 3.2 14.8 1.0 CB B:CYS112 3.2 2.0 1.0 CA B:HIS46 3.2 34.5 1.0 CB B:HIS117 3.4 12.8 1.0 CB B:HIS46 3.4 3.5 1.0 C B:GLY45 3.9 29.6 1.0 N B:HIS46 4.0 8.0 1.0 CB B:PHE114 4.0 2.0 1.0 CE B:MET121 4.1 2.0 1.0 NE2 B:HIS117 4.1 28.1 1.0 NE2 B:HIS46 4.2 17.9 1.0 CD2 B:HIS117 4.2 16.1 1.0 CD2 B:HIS46 4.2 22.0 1.0 C B:HIS46 4.4 2.0 1.0 N B:ASN47 4.5 23.2 1.0 CA B:CYS112 4.6 18.0 1.0 CG B:MET121 4.7 2.0 1.0 CG B:PHE114 4.7 11.3 1.0 O B:MET44 4.9 15.7 1.0 CA B:HIS117 4.9 7.6 1.0 C B:CYS112 5.0 5.5 1.0 N B:PHE114 5.0 14.0 1.0

Copper binding site 3 out of 4 in the X-Ray Crystal Structure of the Two Site-Specific Mutants HIS35GLN and HIS35LEU of Azurin From Pseudomonas Aeruginosa


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 3 of X-Ray Crystal Structure of the Two Site-Specific Mutants HIS35GLN and HIS35LEU of Azurin From Pseudomonas Aeruginosa within 5.0Å range: probe atom residue distance (Å) B Occ C:Cu129 b:17.2 occ:1.00 ND1 C:HIS117 2.0 22.4 1.0 ND1 C:HIS46 2.0 6.5 1.0 SG C:CYS112 2.1 7.2 1.0 CG C:HIS117 3.0 2.9 1.0 CE1 C:HIS117 3.0 2.0 1.0 CE1 C:HIS46 3.0 5.8 1.0 CG C:HIS46 3.0 7.1 1.0 O C:GLY45 3.0 7.7 1.0 SD C:MET121 3.1 12.5 1.0 CB C:CYS112 3.2 3.4 1.0 CA C:HIS46 3.4 2.0 1.0 CB C:HIS117 3.4 13.6 1.0 CB C:HIS46 3.4 2.1 1.0 CE C:MET121 3.8 2.0 1.0 C C:GLY45 3.9 6.9 1.0 CB C:PHE114 4.0 12.5 1.0 N C:HIS46 4.0 5.2 1.0 NE2 C:HIS117 4.1 9.0 1.0 NE2 C:HIS46 4.1 2.1 1.0 CD2 C:HIS117 4.2 12.5 1.0 CD2 C:HIS46 4.2 7.3 1.0 C C:HIS46 4.5 23.1 1.0 CG C:MET121 4.5 12.0 1.0 CA C:CYS112 4.5 2.0 1.0 N C:ASN47 4.6 14.2 1.0 CG C:PHE114 4.7 8.6 1.0 CB C:MET121 4.9 11.8 1.0 CA C:HIS117 4.9 2.0 1.0 C C:CYS112 4.9 10.1 1.0 N C:PHE114 4.9 5.1 1.0

Copper binding site 4 out of 4 in the X-Ray Crystal Structure of the Two Site-Specific Mutants HIS35GLN and HIS35LEU of Azurin From Pseudomonas Aeruginosa


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 4 of X-Ray Crystal Structure of the Two Site-Specific Mutants HIS35GLN and HIS35LEU of Azurin From Pseudomonas Aeruginosa within 5.0Å range: probe atom residue distance (Å) B Occ D:Cu129 b:17.6 occ:1.00 ND1 D:HIS46 2.0 5.0 1.0 SG D:CYS112 2.0 9.3 1.0 ND1 D:HIS117 2.0 25.3 1.0 SD D:MET121 2.9 16.8 1.0 CE1 D:HIS117 3.0 10.5 1.0 CE1 D:HIS46 3.0 69.5 1.0 CG D:HIS46 3.0 41.2 1.0 CG D:HIS117 3.0 10.5 1.0 O D:GLY45 3.2 20.3 1.0 CB D:CYS112 3.3 12.1 1.0 CA D:HIS46 3.4 10.5 1.0 CB D:HIS117 3.4 4.7 1.0 CB D:HIS46 3.4 2.0 1.0 CE D:MET121 3.9 2.4 1.0 CB D:PHE114 3.9 2.7 1.0 C D:GLY45 4.0 13.8 1.0 NE2 D:HIS117 4.1 14.7 1.0 NE2 D:HIS46 4.1 30.8 1.0 N D:HIS46 4.1 5.1 1.0 CD2 D:HIS46 4.2 27.1 1.0 CD2 D:HIS117 4.2 18.2 1.0 CG D:MET121 4.5 6.5 1.0 C D:HIS46 4.5 6.8 1.0 N D:ASN47 4.6 9.4 1.0 CA D:CYS112 4.6 20.1 1.0 CG D:PHE114 4.7 14.3 1.0 N D:PHE114 4.8 13.6 1.0 O D:MET44 4.9 20.2 1.0 CA D:HIS117 4.9 29.0 1.0 CB D:MET121 4.9 22.8 1.0 CA D:PHE114 5.0 14.2 1.0

H.Nar, A.Messerschmidt, R.Huber, M.Van De Kamp, G.W.Canters. X-Ray Crystal Structure of the Two Site-Specific Mutants HIS35GLN and HIS35LEU of Azurin From Pseudomonas Aeruginosa. J.Mol.Biol. V. 218 427 1991.
ISSN: ISSN 0022-2836
PubMed: 1901363
DOI: 10.1016/0022-2836(91)90723-J